http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Salma Ahmed (검색결과 8 건)
- 무료
- 기관 내 무료
- 유료
-
El-Sawi Salma Ahmed,Shahira Mohamed Ezzat,Hanan Farouk Aly,Rana Merghany Merghany,Meselhy Ragab Meselhy 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.3
Salvia splendens is a species of the genus Salvia that is known for its neuro-therapeutic properties. The present study aimed to investigate the effect of two fractions from the methanolic extract of the aerial parts of S. splendens cultivated in Egypt, the petroleum ether-soluble (PES) and n-butanol-soluble (BS) fractions, against AlCl3- induced Alzheimer’s disease (AD) in rats. Rats treated with AlCl3 (100 mg/kg b.wt. p.o.) for 4 weeks developed behavioral, biochemical and histological changes similar to that of AD. Behavioral deficits were assessed by T-maze test and percentage changes in oxidative stress and AD markers in brain. Extent of DNA damage and histopathological changes were also evaluated. Results revealed that both fractions; PES and BS (at dose of 500 mg/kg b.wt), significantly attenuated AlCl3- induced behavioral impairment in rats. This effect was accompanied by acetylcholinesterase (AChE) activity inhibition (53.18% and 68.66%, respectively), and Aβ deposition reduction (33.3% and 34.3%, respectively). Both fractions markedly decreased oxidative stress markers level (lipid peroxide, protein carbonyl, reduced glutathione and nitric oxide), and inhibited catalase and caspase-3 activities. Also, the content of noradrenaline, adrenaline, 5-HT and dopamine were significantly increased. The fractions preserved the histo-architecture pattern of the hippocampus and cortex from the AlCl3- induced damage. Bioactivity-guided fractionation led to the isolation of two sterols; β-sitosterol and β-sitosterolpalmitate from PES fraction, and 6 phenolic compounds (acacetin, chrysoeriol, apigenin, luteolin, rosmarinic acid and caffeic acid) from BS fraction. Rosmarinic acid and caffeic acid significantly inhibited AChE in vitro ( IC50 values of 0.398 mg/mL and 0.327 mg/mL, respectively) compared to physostigmine ( IC50 0.227 mg/ mL). The BS fraction is standardized (HPLC–DAD) to contain not less than 0.0254% (w/w)of rosmarinic acid and 0.0129% (w/w) of caffeic acid. These findings suggest that S. splendens is beneficial in attenuating AlCl3- induced neurotoxicity in rats.
-
-
-
Blocking Toll-like receptor 9 attenuates bleomycin-induced pulmonary injury
Badr Alzahrani,Mohamed M. S. Gaballa,Ahmed A. Tantawy,Maha A. Moussa,Salma A. Shoulah,Said M. Elshafae 대한병리학회 2022 Journal of Pathology and Translational Medicine Vol.56 No.2
Background: Acute respiratory distress syndrome (ARDS) is one of the most common complications in coronavirus disease 2019 patients suffering from acute lung injury (ALI). In ARDS, marked distortion of pulmonary architecture has been reported. The pulmonary lesions in ARDS include hemodynamic derangements (such as alveolar edema and hemorrhage), vascular and bronchiolar damage, interstitial inflammatory cellular aggregations, and eventually fibrosis. Bleomycin induces ARDS-representative pulmonary damage in mice and rats; therefore, we used bleomycin model mice in our study. Recently, Toll-like receptor 9 (TLR9) was implicated in the development of ARDS and ALI.Methods: In this study, we evaluated the efficiency of a TLR9 blocker (ODN2088) on bleomycin-induced pulmonary damage. We measured the apoptosis rate, inflammatory reaction, and fibroplasia in bleomycin- and bleomycin + ODN2088-treated mice.Results: Our results showed a significant amelioration in bleomycin-induced damage to pulmonary architecture following ODN2088 treatment. A marked decrease in pulmonary epithelial and endothelial apoptosis rate as measured by cleaved caspase-3 expression, inflammatory reaction as indicated by tumor necrosis factor α expression, and pulmonary fibrosis as demonstrated by Van Gieson staining and α-smooth muscle actin immunohistochemistry were observed following ODN2088 treatment.Conclusions: All these findings indicate that blocking downstream TLR9 signaling could be beneficial in prevention or mitigation of ARDS through hemodynamic derangements, inflammation, apoptosis, and fibrosis.
-
Ghareeb Doaa A.,Saleh Samar R.,Seadawy Mohamed G.,Nofal Mohammed S.,Abdulmalek Shaymaa A.,Hassan Salma. F.,Khedr Shaimaa M.,AbdElwahab Miral G.,Sobhy Ahmed A.,Abdel-Hamid Ali saber Ali,Yassin Abdelrah 한국약제학회 2021 Journal of Pharmaceutical Investigation Vol.51 No.6
Purpose A novel coronavirus (COVID-19) that has not been previously identified in humans and has no specific treatment has recently spread. Treatment trials using antiviral and immune-modulating drugs such as hydroxychloroquine (HCQ) were used to control this viral outbreak however several side effects have emerged. Berberine (BER) is an alkaloid that has been reported to reveal some pharmacological properties including antioxidant and antimicrobial activities. Additionally, Zinc oxide nanoparticles (ZnO-NPs) possess potent antioxidant and anti-inflammatory properties. Therefore, this study was undertaken to estimate the efficiency of both BER and synthetic ZnO/BER complex as an anti-COVID-19 therapy. Methods First, the ZnO/BER complex was prepared by the facile mixing method. Then in vitro studies on the two compounds were conducted including VeroE6 toxicity, anti-COVID-19 activity, determination of inhibitory activity towards papain-like proteinase (PL pro) and spike protein- and receptor- binding domain (RBD) as well as assessment of drug toxicity on RBCs. Results The results showed that ZnO/BER complex acts as an anti-COVID-19 by inhibiting spike protein binding with angiotensin-converting enzyme II (ACE II), PL pro activity, spike protein and E protein levels, and expression of both E-gene and RNA dependent RNA polymerase (RdRp) at a concentration lower than that of BER or ZnO-NPs alone. Furthermore, ZnO/BER complex had antioxidant and antimicrobial properties where it prevents the auto oxidation of 2,2-Diphenyl- 1-picrylhydrazyl (DPPH) and the culture of lower respiratory system bacteria that affected Covid 19 patients. The ZnO/BER complex prevented as well the HCQ cytotoxic effect on both RBC and WBC (in vitro) and hepatotoxicity, nephrotoxicity and anemia that occurred after HCQ long administration in vivo. Conclusion The ZnO/BER complex can be accounted as promising anti-COVID 19 candidate because it inhibited the virus entry, replication, and assembly. Furthermore, it could be used to treat a second bacterial infection that took place in hospitalized COVID 19 patients. Moreover, ZnO/BER complex was found to eliminate the toxicity of long-term administration of HCQ in vivo.
-
Potential therapeutic and pharmacological strategies for SARS-CoV2
Ghareeb Doaa A.,Saleh Samar R.,Nofal Mohammed S.,Kaddah Mohamed M. Y.,Hassan Salma. F.,Seif Inas K.,El-Zahaby Sally A.,Khedr Shaimaa M.,Kenawy Marwa Y.,Masoud Aliaa A.,Soudi Salma A.,Sobhy Ahmed A.,Se 한국약제학회 2021 Journal of Pharmaceutical Investigation Vol.51 No.3
Background At the end of 2019, the new Coronavirus disease 2019 (COVID-19) strain causing severe acute respiratory syndrome swept the world. From November 2019 till February 2021, this virus infected nearly 104 million, with more than two million deaths and about 25 million active cases. This has prompted scientists to discover effective drugs to combat this pandemic. Area covered Drug repurposing is the magic bullet for treating severe acute respiratory syndrome coronavirus 2 (SARSCoV2). Therefore, several drugs have been investigated in silico, in vitro, as well as through human trials such as anti- SARS-CoV2 agents, or to prevent the complications resulting from the virus. In this review, the mechanisms of action of different therapeutic strategies are summarized. According to the WHO, different classes of drugs can be used, including anti-malarial, antiviral, anti-inflammatory, and anti-coagulant drugs, as well as angiotensin-converting enzyme inhibitors, antibiotics, vitamins, zinc, neutralizing antibodies, and convalescent plasma therapy. Recently, there are some vaccines which are approved against SARS-CoV2. Expert opinion A complete understanding of the structure and function of all viral proteins that play a fundamental role in viral infection, which contribute to the therapeutic intervention and the development of vaccine in order to reduce the mortality rate.
-
Khanam, Farhana,Sheikh, Alaullah,Sayeed, Md. Abu,Bhuiyan, Md. Saruar,Choudhury, Feroza Kaneez,Salma, Umme,Pervin, Shahnaz,Sultana, Tania,Ahmed, Dilruba,Goswami, Doli,Hossain, Md. Lokman,Mamun, K. Z.,C Public Library of Science 2013 PLoS neglected tropical diseases Vol.7 No.7
<▼1><P><B>Background</B></P><P>Rapid and reliable diagnostic assays for enteric (typhoid and paratyphoid) fever are urgently needed. We report the characterization of novel approach utilizing lymphocyte secretions, for diagnosing patients with enteric fever by the TPTest procedure.</P><P><B>Methodology</B></P><P>TPTest detects <I>Salmonella</I>-specific IgA responses in lymphocyte culture supernatant. We utilized TPTest in patients with suspected enteric fever, patients with other illnesses, and healthy controls. We also evaluated simplified modifications of TPTest for adaptation in laboratories with limited facilities and equipment.</P><P><B>Principal Findings</B></P><P>TPTest was positive in 39 (27 typhoid and 12 paratyphoid A) patients confirmed by blood culture and was negative in 74 healthy individuals. Among 32 individuals with other illnesses, 29 were negative by TPTest. Of 204 individuals with suspected enteric fever who were negative by blood culture, 44 were positive by TPTest and the patients were clinically indistinguishable from patients with confirmed bacteremia, except they were more likely to be under 5 years of age. We evaluated simplifications in TPTest, including showing that lymphocytes could be recovered using lysis buffer or buffy coat method as opposed to centrifugation, that incubation of cells at 37°C did not require supplemental CO<SUB>2</SUB>, and that results were available for majority of samples within 24 hours. Positive results by TPTest are transient and revert to negative during convalescence, supporting use of the test in endemic areas. The results can also be read using immunodot blot approach as opposed to ELISA. Since no true gold standard currently exists, we used a number of definitions of true positives and negatives. TPTest had sensitivity of 100% compared to blood culture, and specificity that ranged from 78–97% (73–100, 95% CI), depending on definition of true negative.</P><P><B>Conclusion</B></P><P>The TPTest is useful for identification of patients with enteric fever in an endemic area, and additional development of simplified TPTest is warranted.</P></▼1><▼2><P><B>Author Summary</B></P><P><I>Salmonella enterica</I> serotype Typhi and Paratyphi A are responsible for typhoid and paratyphoid fever respectively and the disease caused is known generally as enteric fever. Appropriate and early diagnosis of the disease is important for initiation of treatment of the patient with a suitable antibiotic. The performance of the available diagnostic methods take time and as well as have low sensitivity and specificity. We describe here an immunodiagnostic assay, the TPTest, which is based on the use of secretions of antibodies from peripheral blood lymphocytes. We describe simplifications of the procedure and show that the assay has as a sensitivity of 100% with a specificity that ranges from 78–97% (73–100, 95% CI) for detecting patients with typhoid and paratyphoid fever in an enteric fever endemic zone. The TPTest uses a small blood volume, and reverts to negative by convalescence, supporting its further development as a relatively low cost assay to diagnose patients with enteric fever in endemic zones.</P></▼2>
-
Tariq Asmhan,Nakhi Fatmah Bin,Salah Fatema,Eltayeb Gabass,Abdulla Ghada Jassem,Najim Noor,Khedr Salma Ahmed,Elkerdasy Sara,Al-Rawi Natheer,Al Kawas Sausan,Mohammed Marwan,Shetty Shishir Ram 대한영상치의학회 2023 Imaging Science in Dentistry Vol.53 No.3
Purpose: Artificial intelligence (AI) is poised to play a major role in medical diagnostics. Periodontal disease is one of the most common oral diseases. The early diagnosis of periodontal disease is essential for effective treatment and a favorable prognosis. This study aimed to assess the effectiveness of AI in diagnosing periodontal bone loss through radiographic analysis. Materials and Methods: A literature search involving 5 databases (PubMed, ScienceDirect, Scopus, Health and Medical Collection, Dentistry and Oral Sciences) was carried out. A specific combination of keywords was used to obtain the articles. The PRISMA guidelines were used to filter eligible articles. The study design, sample size, type of AI software, and the results of each eligible study were analyzed. The CASP diagnostic study checklist was used to evaluate the evidence strength score. Results: Seven articles were eligible for review according to the PRISMA guidelines. Out of the 7 eligible studies, 4 had strong CASP evidence strength scores (7-8/9). The remaining studies had intermediate CASP evidence strength scores (3.5-6.5/9). The highest area under the curve among the reported studies was 94%, the highest F1 score was 91%, and the highest specificity and sensitivity were 98.1% and 94%, respectively. Conclusion: AI-based detection of periodontal bone loss using radiographs is an efficient method. However, more clinical studies need to be conducted before this method is introduced into routine dental practice.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
