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      • KCI등재

        Improved furanocoumarin production in Ruta graveolens L. regenerated via in vitro stem internode cultures

        Sagarika Bohidar,Suchismita Pattanaik,Manikkannan Thiruvanoukkarasu 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        A rapid and efficient in vitro propagation protocolby enhanced multiple shoot proliferation from internodecultures of Ruta graveolens was established. Meanshoot number was maximum (55.83) in Murashige andSkoog (MS) basal medium fortified with 1.0 mg L-1benzyl amino purine and 0.25 mg L-1 indole-3-acetic acid. The elongated shoots rooted within 10–12 days in 1/2-strength MS medium supplemented with 2.0 mg L-1indole 3-butyric acid. About 80 % of the rooted plantletssurvived acclimatization and transfer to the field. Phytochemicalanalysis revealed that micropropagated plantsproduced linear furanocoumarins, characteristic of thespecies, in greater quantities as compared to the in vivogrownplants. The results will facilitate the conservation ofthis valuable medicinal plant and to obtain plants withimproved phytochemical constituents.

      • KCI등재

        Genetic Relationship in Tea Germplasms with Drought Contrasting Traits

        ( Sagarika Das ),( Afruza Zaman ),( Sangeeta Borchetia ),( Madhurjya Gogoi ),( Pritom Chowdhury ),( Jayanta Saikia ),( Hemanta Saikia ),( Buddhadeb Das ),( Tarun Sen Barman ),( Tanoy Bandyopadhyay ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.4

        The objective of this study is to correlate the genetic diversity among tea cultivars with drought tolerance/susceptibility on the basis of morphological, physiological and molecular markers. Twelve highly polymorphic inter simple sequence repeat primers targeting the tea dinucleotide repeats predominantly present in drought stressed transcripts were used to screen out the germplasms into two groups showing drought tolerance and susceptible characteristic with polymorphic information content of 0.3. A similarity index of 0.58 was observed among the cultivars. Morpho-physiological diversity among the cultivars was studied using eighteen morphological and two physiological markers. Correlation studies showed significant association between the ranks of the morpho-physiological distances and molecular genetic distances at P=0.05%. The dinucleotide repeats targeted along with morpho-physiological correlation can be used in tea breeding for drought tolerance as the markers are in sync with the long term field trials based on which the plants were classified for drought trait.

      • SCOPUS
      • SCOPUSKCI등재

        Improved furanocoumarin production in Ruta graveolens L. regenerated via in vitro stem internode cultures

        Bohidar, Sagarika,Pattanaik, Suchismita,Thiruvanoukkarasu, Manikkannan 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

        A rapid and efficient in vitro propagation protocol by enhanced multiple shoot proliferation from internode cultures of Ruta graveolens was established. Mean shoot number was maximum (55.83) in Murashige and Skoog (MS) basal medium fortified with $1.0mgL^{-1}$ benzyl amino purine and $0.25mgL^{-1}$ indole-3-acetic acid. The elongated shoots rooted within 10-12 days in 1/2-strength MS medium supplemented with $2.0mgL^{-1}$ indole 3-butyric acid. About 80 % of the rooted plantlets survived acclimatization and transfer to the field. Phytochemical analysis revealed that micropropagated plants produced linear furanocoumarins, characteristic of the species, in greater quantities as compared to the in vivogrown plants. The results will facilitate the conservation of this valuable medicinal plant and to obtain plants with improved phytochemical constituents.

      • SCOPUSKCI등재

        A Semi-supervised Learning of HMM to Build a POS Tagger for a Low Resourced Language

        Pattnaik, Sagarika,Nayak, Ajit Kumar,Patnaik, Srikanta The Korea Institute of Information and Commucation 2020 Journal of information and communication convergen Vol.18 No.4

        Part of speech (POS) tagging is an indispensable part of major NLP models. Its progress can be perceived on number of languages around the globe especially with respect to European languages. But considering Indian Languages, it has not got a major breakthrough due lack of supporting tools and resources. Particularly for Odia language it has not marked its dominancy yet. With a motive to make the language Odia fit into different NLP operations, this paper makes an attempt to develop a POS tagger for the said language on a HMM (Hidden Markov Model) platform. The tagger judiciously considers bigram HMM with dynamic Viterbi algorithm to give an output annotated text with maximum accuracy. The model is experimented on a corpus belonging to tourism domain accounting to a size of approximately 0.2 million tokens. With the proportion of training and testing as 3:1, the proposed model exhibits satisfactory result irrespective of limited training size.

      • SCIESCOPUS

        Uplift capacity of horizontal anchor plate embedded near to the cohesionless slope by limit analysis

        Bhattacharya, Paramita,Sahoo, Sagarika Techno-Press 2017 Geomechanics & engineering Vol.13 No.4

        The effect of nearby cohesionless sloping ground on the uplift capacity of horizontal strip plate anchor embedded in sand deposit with horizontal ground surface has been studied numerically. The numerical analysis has been carried out by using the lower bound theorem of limit analysis with finite elements and linear optimization. The results have been presented in the form of non-dimensional uplift capacity factor of anchor plate by changing its distance from the slope crest for different slope angles, embedment ratios and angles of soil internal friction. It has been found that the decrease in horizontal distance between the edge of the anchor plate and the slope crest causes a continuous decrease in uplift capacity of anchor plate. The optimum distance is that distance between slope crest and anchor plate below which uplift capacity of an anchor plate has been found to decrease with a decrease in normalized crest distance from the anchor plate in presence of nearby sloping ground. The normalized optimum distance between the slope crest and the anchor plate has been found to increase with an increase in slope angle, embedment ratio and soil internal friction angle.

      • KCI등재

        Effectiveness of online versus in-person structured training program on arterial blood gas, electrolytes, and ventilatory management of critically ill patients

        Gaurav Jain,Bhavna Gupta,Priyanka Gupta,Sagarika Panda,Sameer Sharma,Shalinee Rao 대한중환자의학회 2021 Acute and Critical Care Vol.36 No.1

        Background: Due to the risk of viral transmission during in-person training, a shift towardonline platforms is imperative in the current pandemic. Therefore, we compared the effectivenessof an in-person interactive course with a structurally similar online course designedto improve cognitive skills among clinical health professionals in arterial blood gas analysis,management of electrolyte imbalances, and approaches to mechanical ventilation in criticallyill patients. Methods: In an observational, outcome assessor-blinded, cohort trial, group A included participantsenrolled prospectively in an online course, while group B included those who tookpart in an in-person course (retrospective arm). The primary objective was comparison ofcognitive skills through a pre and post-test questionnaire. Statistical analysis was performedusing Student t-test. Results: In total, 435 participants were analyzed in group A, while 99 participants were evaluatedin group B. The mean pre-test score was 9.48±2.75 and 10.76±2.42, while the meanpost-test score was 11.94±1.90 (passing rate, 64.6%) and 12.53±1.63 (passing rate, 73.3%)in groups A and B, respectively. Group B scored significantly higher in both pre-test (P=0.001)and post-test evaluations (P=0.004). The improvement in post-test score was significantlygreater (P=0.001) in group A (2.46±2.22) compared to group B (1.77±1.76). The medicalspecialties fared better in group B, while surgical specialties scored higher in group A. Thepre-test vs. post-test scores exhibited a moderate correlation in both groups (P<0.001). Thefeedback survey showed a Likert score >3.5 for most points in both groups. Conclusions: The online teaching module exhibited a significant benefit in terms of participantsensitization and knowledge sharing.

      • Hardcore Smoking in Three South-East Asian Countries: Results from the Global Adult Tobacco Survey

        Kishore, Jugal,Jena, Pratap Kumar,Bandyopadhyay, Chandan,Swain, Monali,Das, Sagarika,Banerjee, Indrani Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

        Background: Hardcore smoking is represented by a subset of daily smokers with high nicotine dependence, inability to quit and unwillingness to quit. Estimating the related burden could help us in identifying a high risk population prone to tobacco induced diseases and improve cessation planning for them. This study assessed the prevalence and associated factors of hardcore smoking in three South-East Asian countries and discussed its implication for smoking cessation intervention in this region. Materials and Methods: Global Adult Tobacco Survey (GATS) data of India, Bangladesh and Thailand were analyzed to quantify the hardcore smoking prevalence in the region. On the basis of review, an operational definition of hardcore smoking was adopted that includes (1) current daily smoker, (2) no quit attempt in the past 12 months of survey or last quit attempt of less than 24 hours duration, (3) no intention to quit in next 12 months or not interested in quitting, (4) time to first smoke within 30 minutes of waking up, and (5) knowledge of smoking hazards. Logistic regression analysis was carried out using hardcore smoking status as response variable and gender, type of residence, occupation, education, wealth index and age-group as possible predictors. Results: There were 31.3 million hardcore smokers in the three Asian countries. The adult prevalence of hardcore smoking in these countries ranges between 3.1% in India to 6% in Thailand. These hardcore smokers constitute 18.3-29.7% of daily smokers. The logistic regression model indicated that age, gender, occupation and wealth index are the major predictors of hardcore smoking with varied influence across countries. Conclusions: Presence of a higher number of hardcore smoking populations in Asia is a major public health challenge for tobacco control and cancer prevention. There is need of intensive cessation interventions with due consideration of contextual predictors.

      • Tobacco Use and Quit Behaviour Assessment in the Global Adult Tobacco Survey (GATS): Invalid Responses and Implications

        Jena, Pratap Kumar,Kishore, Jugal,Pati, Sanghamitra,Sarkar, Bidyut Kanti,Das, Sagarika Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.11

        Background: Tobacco use and quit attempts are two key indicators of the Global Adult Tobacco Survey (GATS) that assess quit attempts among current as well as former tobacco users. The relevant data have inherent policy implications for tobacco cessation programme evaluation. This study aimed to review the concepts of quit attempt assessment and quantifying invalid responses considering GATS-India data. Materials and Methods: GATS assessment of tobacco use and quit attempts were examined in the current literature. Two categories of invalid responses were identified by stratified analysis of the duration of last quit attempt among current users and duration of abstinence among former users. Category A included absolute invalid responses when time-frame of assessment of current tobacco use and less than former tobacco use were violated. Category B included responses that violated the unit of measurement of time. Results: Current daily use, current less than daily use and former use in GATS were imprecisely defined with overlapping of time-frame of assessment. Overall responses of 3,102 current smokers, 4,036 current smokeless users, 1,904 former smokers and 1,343 former smokeless users were analyzed to quantify invalid responses. Analysis indicated overall 21.2% (category A: 7.32%; category B: 17.7%) and 22.7% (category A: 8.05%; category B: 18.1%) invalid responses among current smokers and smokeless users respectively regarding their duration of last quit attempt. Similarly overall 6.62% (category A: 4.7%; category B: 2.3%) and 10.6% (category A: 8.6%; category B: 3.5%) invalid responses were identified among former smokers and smokeless users respectively regarding their duration of abstinence. Conclusions: High invalid responses for a single assessment are due to the imprecise definition of current use, former use and quit attempt; and failure to utilize opportunity of direct data entry interface use during the survey to validate responses instantly. Redefining tobacco use and quit attempts considering an appropriate timeframe would reduce invalid responses.

      • Extending Application of the 'Hardcore' Definition to Smokeless Tobacco Use: Estimates from a Nationally Representative Population in India and its Implications

        Jena, Pratap Kumar,Bandyopadhyay, Chandan,Mathur, Manu Raj,Das, Sagarika Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

        Background: The term 'hardcore' has been applied to use of smoking tobacco and generally referred to as the inability or unwillingness of regular smokers to quit. The component constructs of hardcore except nicotine dependence are product neutral. With the use of 'time to first chew' as a measure of nicotine dependence, hardcore definition can be extended to characterize smokeless tobacco users. Hardcore users respond less to tobacco cessation interventions, and are prone to tobacco induced diseases including cancer. Thus identifying hardcore users would help in estimate the burden of high risk population for tobacco induced diseases. Smokeless tobacco use is predominant and accounts for more than 50% of oral cancer in India. Hence, hardcore chewing information could be used for planning of tobacco and cancer control interventions. The objective of this study was to assess the prevalence and associated factors of hardcore smokeless tobacco use in India. Materials and Methods: Global Adult Tobacco Survey (GATS)-India 2010 data were analyzed to quantify hardcore smokeless tobacco use in India with following five criteria: (1) current daily smokeless tobacco use; (2) no quit attempt in the past 12 months of survey or last quit attempt of less than 24 hours duration; (3) no intention to quit in next 12 months or not interested in quitting; (4) time to first use of smokeless tobacco product within 30 minutes of waking up; and (5) knowledge of smokeless tobacco hazards. Results: The number of hardcore smokeless tobacco users among adult Indians is estimated to be 5% (39.5 million). This group comprises 23.2% of daily smokeless tobacco users. The population prevalence varied from 1.4-9.1% across different national regions of India. Logistic regression modeling indicated age, education and employment status to be the major predictors of hardcore smokeless tobacco use in India. Conclusions: The presence of a huge number (39.5 million) of hardcore smokeless tobacco users is a challenge to tobacco control and cancer prevention in India. There is an unmet need for a universal tobacco cessation programme and intensification of anti-tobacco education in communities.

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