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- 저자 : ( Buddhadeb Das ) (검색결과 2 건)
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Genetic Relationship in Tea Germplasms with Drought Contrasting Traits
( Sagarika Das ),( Afruza Zaman ),( Sangeeta Borchetia ),( Madhurjya Gogoi ),( Pritom Chowdhury ),( Jayanta Saikia ),( Hemanta Saikia ),( Buddhadeb Das ),( Tarun Sen Barman ),( Tanoy Bandyopadhyay ) 한국육종학회 2016 Plant Breeding and Biotechnology Vol.4 No.4
The objective of this study is to correlate the genetic diversity among tea cultivars with drought tolerance/susceptibility on the basis of morphological, physiological and molecular markers. Twelve highly polymorphic inter simple sequence repeat primers targeting the tea dinucleotide repeats predominantly present in drought stressed transcripts were used to screen out the germplasms into two groups showing drought tolerance and susceptible characteristic with polymorphic information content of 0.3. A similarity index of 0.58 was observed among the cultivars. Morpho-physiological diversity among the cultivars was studied using eighteen morphological and two physiological markers. Correlation studies showed significant association between the ranks of the morpho-physiological distances and molecular genetic distances at P=0.05%. The dinucleotide repeats targeted along with morpho-physiological correlation can be used in tea breeding for drought tolerance as the markers are in sync with the long term field trials based on which the plants were classified for drought trait.
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Das, Subhasish,Teja, K. Charan,Mukherjee, Sandip,Seal, Soma,Sah, Rajesh Kumar,Duary, Buddhadeb,Kim, Ki-Hyun,Bhattacharya, Satya Sundar Elsevier 2018 Environmental research Vol.161 No.-
<P><B>Abstract</B></P> <P>Carlinoside is a unique compound well-known for its excellent curative potential in hepatitis. There is a substantial research gap regarding the medicinal use of carlinoside, as its concentrations are greatly variable (depending on locality). We cultivated <I>Cajanus cajan</I> using vermicompost as a major organic amendment at two locations (Sonitpur and Birbhum) with different soil types, but identical climate conditions. Sonitpur soils were richer in soil organic C (SOC), enzyme activation, and N/P content than Birbhum. However, vermi-treatment improved many soil properties (bulk density, water retention, pH, N/P/K, and enzyme activity) to narrow the locational gap in soil quality by 15–28%. We also recorded a many-fold increment in SOC storage capacities in both locations, which was significantly correlated with carlinoside, total phenol, and flavonoid contents in <I>Cajanus</I> leaves. This significantly up-regulated the carlinoside induced expression of the bilirubin-solubilizing UGT1A1enzyme in HepG2 cell and rat liver. Leaf extracts of vermicompost-aided plants could cure hepatitis in affected rat livers and in the HepG2 cell line. Accordingly, vermi-treatment is an effective route for the growth of <I>Cajanus</I> as a cash crop for biomedical applications and can produce a concurrent improvement in soil quality.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Soil organic C primarily induces carlinoside biosynthesis in <I>Cajanus cajan</I>. </LI> <LI> Vermicompost elevates the medicinal value of <I>Cajanus cajan</I> against hepatitis. </LI> <LI> Vermi-treated <I>Cajanus</I> yield carlinoside stably irrespective of growing location. </LI> <LI> Vermi-treated leaf extracts expedited bilirubin solubilization in rat liver. </LI> </UL> </P>
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