http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Siddikee M. A. ),( P. S. Chauhan ),( R. Anandham ),( Gwang Hyun Han ),( Tong Min Sa ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
In total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the Yellow Sea, near the city of Incheon in the Republic of Korea. All of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (IAA), nitrogen fixation, phosphorus (P) and zinc (Zn) solubilization, thiosulfate (S2O3) oxidation, the production of ammonia (NH3), and the production of extracellular hydrolytic enzymes such as protease, chitinase, pectinase, cellulase, and lipase under in vitro conditions. From the original 140 strains tested, on the basis of the latter tests for plant growth promotional activity, 36 were selected for further examination. These 36 halotolerant bacterial strains were then tested for 1- aminocyclopropane-1-carboxylic acid (ACC) deaminase activity. Twenty-five of these were found to be positive, and to be exhibiting significantly varying levels of activity. 16S rRNA gene sequencing analyses of the 36 halotolerant strains showed that they belong to 10 different bacterial genera: Bacillus, Brevibacterium, Planococcus, Zhihengliuella, Halomonas, Exiguobacterium, Oceanimonas, Corynebacterium, Arthrobacter, and Micrococcus. Inoculation of the 14 halotolerant bacterial strains to ameliorate salt stress (150 mM NaCl) in canola plants produced an increase in root length of between 5.2% and 47.8%, and dry weight of between 16.2% and 43%, in comparison with the uninoculated positive controls. In particular, three of the bacteria, Brevibacterium epidermidis RS15, Micrococcus yunnanensis RS222, and Bacillus aryabhattai RS341, all showed more than 40% increase in root elongation and dry weight when compared with uninoculated saltstressed canola seedlings. These results indicate that certain halotolerant bacteria, isolated from coastal soils, have a real potential to enhance plant growth under saline stress, through the reduction of ethylene production via ACC deaminase activity.
Abhishek S. Mahajan,Young Gun Kim,Jae Hwa Kim,Prashanth D’sa,Azhar Lakhani,Hyun Soo Ok 대한정형외과학회 2016 Clinics in Orthopedic Surgery Vol.8 No.4
Background: To assess the functional and radiological outcomes of minimally invasive anterior bridge plating (ABP) for mid-shaft humerus fractures in patients predominantly involved in overhead activities (athletes and manual laborers). Methods: Forty-eight patients fulfilling inclusion criteria were treated with ABP at a level-I trauma center using a 4.5-mm dynamic/ locking compression plate and followed for a period of 1 year. Functional outcome was assessed using the Constant, Mayo elbow, and Disabilities of the Arm, Shoulder and Hand (DASH) scores. Range of motion (ROM), subjective strength, and radiographic union were assessed. A general satisfaction questionnaire was also administered. Results: Most patients achieved excellent functional and radiological outcomes. ROM and strength of the shoulder girdle exhibited clinically nonsignificant loss as compared to the opposite side. The mean time for return to the original activities was 64 days (range, 36 to 182 days) and the mean time for confirmed radiographic union was 45 days (range, 34 to 180 days). The mean Constant, Mayo elbow, DASH scores were 95.73 ± 5.76 (range, 79 to 100), 95.94 ± 6.74 (range, 85 to 100), and 1.56 ± 3.15 (range, 0.0 to 14.0), respectively. The majority of patients (43 patients, 89.6%) who fell in the excellent or very good category according to our questionnaire were extremely satisfied. There were 2 cases (4.17%) of nonunion and 3 patients (6.25%) had to change/modify their original occupation. Conclusions: ABP is fundamentally different from traditional open posterior plating or conventional intramedullary nailing. It gives relative stability with union taking place by callus formation, and a longer plate on the tensile surface ensures that the humerus can withstand greater amount of rotational and bending stresses. The minimally invasive nature causes minimal soft tissue damage and, if done correctly, causes no damage to the vital structures in proximity. ABP for mid-shaft humerus fractures in patients predominantly engaged in overhead activities is a safe and effective treatment modality yielding high rates of union, excellent functional recovery, minimal biological disruption, better cosmesis, and superior satisfaction rates.
Hari P. Deka Boruah,Puneet S. Chauhan,Woo-Jong Yim(임우종),Gwang-Hyun Han(한광현),Tong-Min Sa(사동민) 한국토양비료학회 2010 한국토양비료학회지 Vol.43 No.1
무균 및 온실조건에서 indole-3-acetic acid (IAA)의 처리와 1-aminocyclopropane-1-carboxylate deaminase (ACCD) 및 IAA 활성을 갖는 Methylobacterium 균주 접종 시 토마토와 고추의 생장을 비교 평가하였다. 무균조건에서 1.0 ㎍ mL?¹의 IAA는 고추와 토마토의 뿌리생장을 촉진시키는데 비해 10.0 ㎍ mL?¹ 이상의 높은 농도에서는 뿌리생장이 억제되었다. 그러나 높은 ACCD 활성을 갖고, IAA 활성은 낮거나 가지고 있지 않은 Methylobacterium 균주들을 접종하였을 때에는 고추와 토마토 모두 IAA 처리구 보다 뿌리생장이 증진되는 것을 확인하였다. 마찬가지로 온실조건에서 Methylobacterium 균주들을 접종했을 때, 마디길이와 잎의 크기 그리고 단위 면적당 잎의 무게(SLW)에서 유의성 있는 증진효과를 보였다. 전반적인 식물 생장에서 저농도의 IAA 처리 효과는 Methylobacterium의 효과와 비슷한 경향을 나타냈다. 유묘의 지상부 길이는 ACCD 활성과 IAA 생산능을 갖는 Methylobacterium 균주 처리구에서 유의성 있는 증가를 확인할 수 있었으며, 전체 건물중 또한 Methylobacterium 처리 시 유의성 있는 증진 효과를 확인 할 수 있었다. 하지만 고농도의 IAA는 고추와 토마토의 생물량을 억제시켰다. 이러한 결과는 접종 균주의 IAA와 ACCD가 고추와 토마토 유묘의 생장 증진에 영향을 끼친다는 것을 증명한다. A comparative study was performed in gnotobiotic and greenhouse conditions to evaluate the effect of exogenous application of indole-3-acetic acid (IAA) and inoculation of Methylobacterium spp. possessing 1-aminocyclopropane-1-carboxylate deaminase (ACCD) and IAA activity on red pepper and tomato seedling growth and development. Application of 1.0 ㎍ mL?¹ IAA positively influenced root growth while high concentrations (>10.0 ㎍ mL?¹) suppressed root growth of red pepper and tomato under gnotobiotic condition. On the other hand, inoculation of Methylobacterium strains with ACCD activity and IAA or without IAA enhanced root growth in both plants. Similarly, under greenhouse condition the inoculation of Methylobacterium sp. with ACCD activity and IAA enhanced plant fitness recorded as average nodal length and specific leaf weight (SLW) but the effect is comparable with the application of low concentrations of IAA. Seedling length was significantly increased by Methylobacterium strains while total biomass was enhanced by Methylobacterium spp. and exogenous applications of < 10.0 ㎍ mL?¹ IAA. High concentrations of IAA retard biomass accumulation in red pepper and tomato. These results confirm that bacterial strains with plant growth promoting characters such as IAA and ACCD have characteristic effects on different aspects of growth of red pepper and tomato seedlings which is comparable or better than exogenous applications of synthetic IAA.
Pharmacogenomic landscape of patient-derived tumor cells informs precision oncology therapy
Lee, Jin-Ku,Liu, Zhaoqi,Sa, Jason K.,Shin, Sang,Wang, Jiguang,Bordyuh, Mykola,Cho, Hee Jin,Elliott, Oliver,Chu, Timothy,Choi, Seung Won,Rosenbloom, Daniel I. S.,Lee, In-Hee,Shin, Yong Jae,Kang, Hyun J Nature Pub. Co 2018 Nature genetics Vol.50 No.10
Spatiotemporal genomic architecture informs precision oncology in glioblastoma
Lee, Jin-Ku,Wang, Jiguang,Sa, Jason K,Ladewig, Erik,Lee, Hae-Ock,Lee, In-Hee,Kang, Hyun Ju,Rosenbloom, Daniel S,Camara, Pablo G,Liu, Zhaoqi,van Nieuwenhuizen, Patrick,Jung, Sang Won,Choi, Seung Won,Ki Nature Pub. Co 2017 Nature genetics Vol.49 No.4
<P>Precision medicine in cancer proposes that genomic characterization of tumors can inform personalized targeted therapies1-5. However, this proposition is complicated by spatial and temporal heterogeneity6-14. Here we study genomic and expression profiles across 127 multisector or longitudinal specimens from 52 individuals with glioblastoma (GBM). Using bulk and single-cell data, we find that samples from the same tumor mass share genomic and expression signatures, whereas geographically separated, multifocal tumors and/or long-term recurrent tumors are seeded from different clones. Chemical screening of patient-derived glioma cells (PDCs) shows that therapeutic response is associated with genetic similarity, and multifocal tumors that are enriched with PIK3CA mutations have a heterogeneous drug-response pattern. We show that targeting truncal events is more efficacious than targeting private events in reducing the tumor burden. In summary, this work demonstrates that evolutionary inference from integrated genomic analysis in multisector biopsies can inform targeted therapeutic interventions for patients with GBM.</P>