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Kim, H M,Lee, B R,Lee, E S,Kwon, M H,Huh, J H,Kwon, B-E,Park, E-K,Chang, S-Y,Kweon, M-N,Kim, P-H,Ko, H-J,Chung, C H Nature Publishing Group 2018 International Journal of Obesity Vol.42 No.2
<P>Non-alcoholic fatty liver disease and non-alcoholic steatohepatitis are characterized by an increase in hepatic triglyceride content with infiltration of immune cells, which can cause steatohepatitis and hepatic insulin resistance. C-C chemokine receptor 7 (CCR7) is primarily expressed in immune cells, and CCR7 deficiency leads to the development of multi-organ autoimmunity, chronic renal disease and autoimmune diabetes. Here, we investigated the effect of CCR7 on hepatic steatosis in a mouse model and its underlying mechanism. Our results demonstrated that body and liver weights were higher in the CCR7<SUP>−/−</SUP> mice than in the wild-type (WT) mice when they were fed a high-fat diet. Further, glucose tolerance and insulin sensitivity were markedly diminished in CCR7<SUP>−/−</SUP> mice. The number of invariant natural killer T (iNKT) cells was reduced in the livers of the CCR7<SUP>−/−</SUP> mice. Moreover, liver inflammation was detected in obese CCR7<SUP>−/−</SUP> mice, which was ameliorated by the adoptive transfer of hepatic mononuclear cells from WT mice, but not through the transfer of hepatic mononuclear cells from CD1d<SUP>−/−</SUP> or interleukin-10-deficient (IL-10<SUP>−/−</SUP>) mice. Overall, these results suggest that CCR7<SUP>+</SUP> mononuclear cells in the liver could regulate obesity-induced hepatic steatosis via induction of IL-10-expressing iNKT cells.</P>
Nguyen, P.,Kim, K.Y.,Kim, A.Y.,Kim, N.S.,Kweon, H.,Ji, S.D.,Koh, Y.H. 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2
<P>Recent improvements in hygiene and innovations in medical technology have dramatically extended the average lifespan of humans in developed countries. However, extension of lifespan does not correlate with increased healthspan. In this study, we examined the health benefits of mature silk worm larvae enriched with diverse functional materials. We found that Drosophila reared on food containing boiled and freeze-dried mature silk worm larval powder (BMSPF) showed a significantly increased healthspan with an extended lifespan compared to normal food (NF) flies. In addition, BMSPF flies showed a significantly increased resistance to rotenone induced Parkinson's disease symptoms compared with NF flies. The results from microarray gene profiling, quantitative real-time PCR (QRT-PCR), and odorant response assays suggested that BMSPF flies may have improved olfactory responses compared with NF flies. In addition, integrity of mitochondria was significantly enhanced in BMSPF flies. Taken together, our data suggest that BMSP can be used to extend healthspan and prevent onset and progression of PD symptoms induced in Drosophila. (C) 2016 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.</P>
INFLUENCE OF THE MIXING RATIO OF DOUBLE COMPONENTIAL FUELS ON HCCI COMBUSTION
S. SATO,S. P. KWEON,D. YAMASHITA,N. IIDA 한국자동차공학회 2006 International journal of automotive technology Vol.7 No.3
For practical application on the HCCI engine, the solution of subjects, such as control of auto-ignition timing and avoidance of knocking, is indispensable. This study focused on the technique of controlling HCCI combustion appropriately, changing the mixture ratio of two kinds of fuel. Methane and DME/n-Butane were selected as fuels. The influences, which the mixing ratio of two fuels does to ignition timing, ignition temperature, rate of heat release and oxidation reaction process, were investigated by experiment with 4-stroke HCCI engine and numerical calculation with elementary reactions.
INFLUENCE OF THE MIXING RATIO OF DOUBLE COMPONENTIAL FUELS ON HCCI COMBUSTION
Sato, S.,Kweon, S.P.,Yamashita, D.,Iida, N. The Korean Society of Automotive Engineers 2006 International journal of automotive technology Vol.7 No.3
For practical application on the HCCI engine, the solution of subjects, such as control of auto-ignition timing and avoidance of knocking, is indispensable. This study focused on the technique of controlling HCCI combustion appropriately, changing the mixture ratio of two kinds of fuel. Methane and DME/n-Butane were selected as fuels. The influences, which the mixing ratio of two fuels does to ignition timing, ignition temperature, rate of heat release and oxidation reaction process, were investigated by experiment with 4-stroke HCCI engine and numerical calculation with elementary reactions.
Simultaneous integration of multiple genes into the Kluyveromyces marxianus chromosome
Heo, P.,Yang, T.J.,Chung, S.C.,Cheon, Y.,Kim, J.S.,Park, J.B.,Koo, H.M.,Cho, K.M.,Seo, J.H.,Park, J.C.,Kweon, D.H. Elsevier Science Publishers 2013 Journal of biotechnology Vol.167 No.3
While Kluyveromyces marxianus is a promising yeast strain for biotechnological applications, genetic engineering of this strain is still challenging, especially when multiple genes are to be transformed. Sequential gene integration, which takes advantage of repetitive insertion/excision of the URA3 gene as a marker, has been the best option until now, because the URA3-deletion mutant is the only precondition for this method. However, we found that the introduced gene is co-excised during the URA3 excision step for next gene introduction, resulting in a very low cumulative probability (<1.57x10<SUP>-6</SUP>% for 4 genes) of integrating all genes of interest. To overcome this extremely low probability, and to reduce labor and time, all 4 genes were simultaneously transformed. Surprisingly, the infamously high 'non-homologous end joining' activity of K. marxianus enabled simultaneous integration of all 4 genes in a single step, with a probability of 7.9%. Various K. marxianus strains could also be similarly transformed. Our finding not only reduces the labor and time required for such procedures, but also removes a number of preconditions, such as pre-made vectors, selection markers and knockout mutants, which are needed to introduce many genes into K. marxianus.
A biosynthetic pathway for hexanoic acid production in Kluyveromyces marxianus
Cheon, Y.,Kim, J.S.,Park, J.B.,Heo, P.,Lim, J.H.,Jung, G.Y.,Seo, J.H.,Park, J.H.,Koo, H.M.,Cho, K.M.,Park, J.B.,Ha, S.J.,Kweon, D.H. Elsevier Science Publishers 2014 Journal of biotechnology Vol.182 No.-
Hexanoic acid can be used for diverse industrial applications and is a precursor for fine chemistry. Although some natural microorganisms have been screened and evolved to produce hexanoic acid, the construction of an engineered biosynthetic pathway for producing hexanoic acid in yeast has not been reported. Here we constructed hexanoic acid pathways in Kluyveromyces marxianus by integrating 5 combinations of seven genes (AtoB, BktB, Crt, Hbd, MCT1, Ter, and TES1), by which random chromosomal sites of the strain are overwritten by the new genes from bacteria and yeast. One recombinant strain, H4A, which contained AtoB, BktB, Crt, Hbd, and Ter, produced 154mg/L of hexanoic acid from galactose as the sole substrate. However, the hexanoic acid produced by the H4A strain was re-assimilated during the fermentation due to the reverse activity of AtoB, which condenses two acetyl-CoAs into a single acetoacetyl-CoA. This product instability could be overcome by the replacement of AtoB with a malonyl CoA-acyl carrier protein transacylase (MCT1) from Saccharomyces cerevisiae. Our results suggest that Mct1 provides a slow but stable acetyl-CoA chain elongation pathway, whereas the AtoB-mediated route is fast but unstable. In conclusion, hexanoic acid was produced for the first time in yeast by the construction of chain elongation pathways comprising 5-7 genes in K. marxianus.