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Ryo Matsuda,Takanori Kushibiki,Naomichi Fujiuchi,Kazuhiro Fujiwara 한국원예학회 2018 Horticulture, Environment, and Biotechnology Vol.59 No.4
Deconstructed viral vector systems for large-scale production of recombinant proteins in Nicotiana benthamiana plants require Agrobacterium tumefaciens-assisted delivery into mesophyll cells by vacuum infiltration of leaves. To clarify the importance of uniform infiltration over the leaf surface and to propose a possible method for uniform infiltration, we quantified the extent of leaf infiltration and evaluated the potential effect of uniform infiltration on recombinant protein yield. We also investigated the effects of plant characteristics (e.g., plant age, leaf dry mass per area) and leaf detachment treatment on the extent of infiltration. First, a simple method was developed to measure the extent of leaf infiltration using a red dye solution. The quantitative results showed that the extent of infiltration in young and old leaves was substantially lower than in mature leaves. However, recombinant hemagglutinin (HA), an influenza vaccine antigen, accumulated in the infiltrated area of young and old leaves, indicating that they can synthesize and accumulate HA at detectable levels. The extent of infiltration was affected by the plant age but not by leaf dry mass per area. Improving the extent of infiltration by supplemental syringe infiltration significantly increased total HA content in leaves. Thus, increasing the infiltrated leaf area represents a potential strategy for increasing the recombinant protein yield in deconstructed viral vector-based transient gene expression systems with A. tumefaciens. The extent of infiltration was also improved without the need for time-consuming syringe infiltration when detached leaves were subjected to vacuum infiltration, suggesting that this may be a potential method to increase the extent of infiltration.
Recording and Characterization of EEGs by Using Wearable EEG Device
Ryo Inoue,Takenao Sugi,Yoshitaka Matsuda,Satoru Goto,Haruhiko Nohira,Ryuzo Mase 제어로봇시스템학회 2019 제어로봇시스템학회 국제학술대회 논문집 Vol.2019 No.10
Electroencephalographic (EEG) record provides the state of the brain activations and is useful for the clinical diagnosis of the brain dysfunction. However, the preparation of EEG recording such as attachment of electrodes, measurement device setting, etc., is time-consuming and complex. In addition, interpretation of recorded EEG requires particular knowledge and experiences. Therefore, the use of EEG recording is limited. Recent years, simple and easy wearable EEG devices have been developed and are considered to be the practical level. Those devices are easy to attach the measurement electrodes to the scalp without special skills and do not constrain the subjects` movement. In contrast to conventional EEG recordings, the number of electrodes is limited, so the accurate interpretation and/or analysis of EEG characteristics would rather be difficult. Especially, discrimination of actual EEG activities from various contaminated artifacts are crucial for clinical application. In this study, the characteristics of the recorded EEG by using a wearable EEG device was analyzed. Automatic detection method for contaminated artifacts such eye blinking, lateral eye movement (LEM) and electromyographic (EMG) activity were developed. Results were compared with the visual inspection for the recorded data.
Liyao Yu,Kazuhiro Fujiwara,Ryo Matsuda 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.1
Leaves respond to the photosynthetic photon fl ux density (PPFD) and accordingly adjust their morphological and physiologicalproperties, including leaf mass per area (LMA) and the chlorophyll (chl) a/b ratio. These responses are called light acclimationresponses. Although the properties that are responsive to high or low constant PPFD levels have been well described,the extent of the responses to changing PPFD, as observed in open fi elds and greenhouses, remains insuffi ciently quantifi ed. In this study, we quantitatively investigated the eff ects of changes among three PPFD levels (100, 400, and 700 μmol m −2 s −1 )over six days (constant within each day) on leaf light acclimation responses in cucumber ( Cucumis sativus ) seedlings. Weintroduced a time-weighted average PPFD ( L w ), in which the eff ect of the PPFD in the earlier growth period was given lessweight, and demonstrated a signifi cant linear regression of parameters of the leaf light acclimation responses including theLMA, chl a / b ratio, maximum rate of carboxylation capacity of ribulose-1,5-bisphosphate carboxylase/oxygenase ( V c max ) andmaximum rate of electron transport ( J max ) on L w . The result showed that the linear regression model with L w well explainedthe light acclimation responses of leaves to the day-to-day changes in daily PPFD and it estimated these response parametersmore accurately than a model with an average PPFD.