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        Apoptosis and autophagy of muscle cell during pork postmortem aging

        Li Chunmei,Yin Xialian,Xue Panpan,Wang Feng,Song Ruilong,Song Qi,Su Jiamin,Zhang Haifeng 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.2

        Objective: Pork is an important source of animal protein in many countries. Subtle physiochemical changes occur during pork postmortem aging. The changes of apoptosis and autophagy in pork at 6 h to 72 h after slaughter were studied to provide evidence for pork quality. Methods: In this article, morphological changes of postmortem pork was observed through Hematoxylin-eosin staining, apoptotic nuclei were observed by TdT-mediated dUTP nick end labeling assay, protein related to apoptosis and autophagy expressions were tested by western blot and LC3 level were expressed according to immunofluorescence assay. Results: In this study, we found the occurrence of apoptosis in postmortem pork, and the process was characterized by nucleus condensation and fragmentation, formation of apoptotic bodies, increase in apoptosis-related Bax/Bcl-2 levels, and activation of caspases. Autophagy reached its peak between 24 and 48 h after slaughter, accompanied by the formation of autophagosomes on the cell membrane and expression of autophagy-related proteins beclin-1, P62, LC3-I, LC3-II, and ATG5. Conclusion: Obvious apoptosis was observed at 12 h and autophagy reached its peak at 48 h. The present work provides the evidence for the occurrence of apoptosis and autophagy during postmortem aging of pork. In conclusion, the apoptosis and autophagy of muscle cells discovered in this study have important implications for pork in the meat industry.

      • KCI등재

        Enhanced remote-sensing scale for wind damage assessment

        Jianjun Luo,Daan Liang,Cagdas Kafali,Ruilong Li,Tanya M. Brown 한국풍공학회 2014 Wind and Structures, An International Journal (WAS Vol.19 No.3

        This study has developed an Enhanced Remote-Sensing (ERS) scale to improve the accuracy and efficiency of using remote-sensing images of residential building to predict their damage conditions. The new scale, by incorporating multiple damage states observable on remote-sensing imagery, substantially reduces measurement errors and increases the amount of information retained. A ground damage survey was conducted six days after the Joplin EF 5 tornado in 2011. A total of 1,400 one- and two-family residences (FR12) were selected and their damage states were evaluated based on Degree of Damage (DOD) in the Enhanced Fujita (EF) scale. A subsequent remote-sensing survey was performed to rate damages with the ERS scale using high-resolution aerial imagery. Results from Ordinary Least Square regression indicate that ERS-derived damage states could reliably predict the ground level damage with 94% of variance in DOD explained by ERS. The superior performance is mainly because ERS extracts more information. The regression model developed can be used for future rapid assessment of tornado damages. In addition, this study provides strong empirical evidence for the effectiveness of the ERS scale and remote-sensing technology for assessment of damages from tornadoes and other wind events.

      • SCIESCOPUS

        Enhanced remote-sensing scale for wind damage assessment

        Luo, Jianjun,Liang, Daan,Kafali, Cagdas,Li, Ruilong,Brown, Tanya M. Techno-Press 2014 Wind and Structures, An International Journal (WAS Vol.19 No.3

        This study has developed an Enhanced Remote-Sensing (ERS) scale to improve the accuracy and efficiency of using remote-sensing images of residential building to predict their damage conditions. The new scale, by incorporating multiple damage states observable on remote-sensing imagery, substantially reduces measurement errors and increases the amount of information retained. A ground damage survey was conducted six days after the Joplin EF 5 tornado in 2011. A total of 1,400 one- and two-family residences (FR12) were selected and their damage states were evaluated based on Degree of Damage (DOD) in the Enhanced Fujita (EF) scale. A subsequent remote-sensing survey was performed to rate damages with the ERS scale using high-resolution aerial imagery. Results from Ordinary Least Square regression indicate that ERS-derived damage states could reliably predict the ground level damage with 94% of variance in DOD explained by ERS. The superior performance is mainly because ERS extracts more information. The regression model developed can be used for future rapid assessment of tornado damages. In addition, this study provides strong empirical evidence for the effectiveness of the ERS scale and remote-sensing technology for assessment of damages from tornadoes and other wind events.

      • KCI등재

        Endothelial Progenitor Cells Correlated with Oxidative Stress after Mild Traumatic Brain Injury

        Xintao Huang,Dahai Wan,Yunpeng Lin,Naizhao Xue,Jiehe Hao,Ning Ma,Xile Pei,Ruilong Li,Wenju Zhang 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.5

        Purpose: Endothelial progenitor cells (EPCs) play a key role in tissue repair and regeneration. Previous studies have shown that infusion of human umbilical cord blood-derived endothelial colony-forming cells improves outcomes in mice subjected to experimentaltraumatic brain injury (TBI). However, the efficiency of cell transplantation is not satisfactory. Oxidative stress plays a significant role in the survival of transplanted cells following ischemic reperfusion injury. This observational clinical study investigatedthe correlation between the number of circulating EPCs and plasma levels of superoxide dismutase (SOD) and malonyldialdehyde(MDA). Materials and Methods: Peripheral blood samples were collected from 20 patients with mild TBI at day-1, day-2, day-3, day-4, and day-7 post TBI. The number of circulating EPCs and the plasma levels of SOD and MDA were measured. Results: The average of circulating EPCs in TBI patients decreased initially, but increased thereafter, compared with healthy controls. Plasma levels of SOD in TBI patients were significantly lower than those in healthy controls at day-4 post-TBI. MDA levels showed no difference between the two groups. Furthermore, when assessed on day-7 post-TBI, the circulating EPC number were correlated with the plasma levels of SOD and MDA. Conclusion: These results suggest that the number of circulating EPCs is weakly to moderately correlated with plasma levels of SOD and MDA at day-7 post-TBI, which may offer a novel antioxidant strategy for EPCs transplantation after TBI.

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