Adsorptive Recovery and Purification of Prodigiosin from Methanol/Water Solutions of Serratia marcescens Fermentation Broth

Ruey Shin Juang,Chun-Lan Yeh 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1

The use of macroporous polymeric adsorptionresins for the recovery and purification of prodigiosin fromfermentation broth of Serratia marcescens SMDR wassystematically studied, in which the broth was pretreatedby coagulation with alum and the resulting precipitate wasleached by methanol/water solution. Of the seven resinstested, Diaion HP-20 resin was selected considering theadsorption and desorption abilities for prodigiosin at thesame time. The optimal compositions of liquid phases foradsorption and desorption were also examined. Batchexperiments were performed at 15 ~ 35oC by varying initialprodigiosin concentration in solution (0.05 ~ 0.5 mmol/L),in which molar fraction of each component in the solutionwas kept constant. The Freundlich and Langmuir equationswere used to describe adsorption isotherms and the relatedthermodynamic functions were also determined. Fixed-bedexperiments were finally conducted to obtain the breakthroughcharacteristics for the adsorption and desorption ofprodigiosin. Under optimized conditions, a purificationfactor of prodigiosin of 11.4 could be obtained from thepretreated broth after one adsorption-desorption run in fixedbed. The present results had demonstrated the promisingpotential of HP-20 resins for the recovery and purificationof prodigiosin from methanol/water solution of Serratiamarcescens fermentation broth.

Improved Biosorption of Phenol Using Crosslinked Chitosan Beads after Modification with Histidine and Saccharomyces cerevisiae

My Linh Nguyen,Ruey-Shin Juang 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.3

The ability of chitosan beads modified with histidine and Saccharomyces cerevisiae using glutaraldehyde as a crosslinking agent (SC-HIS-CCBs) for the biosorption of phenol was studied. The surface of SC-HIS-CCBs was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) analysis. Batch biosorption experiments using the SC-HIS-CCBs were then performed by varying contact time, solution pH (2.0 ~ 7.0), and initial phenol concentration (25 ~ 250 mg/L). The isotherm and kinetic data were fitted by suitable equations including the Langmuir, Freundlich, pseudo-firstorder, and pseudo-second-order equations. Thermodynamic parameters of phenol biosorption on the SC-HIS-CCBs were calculated. Desorption of phenol from the loaded SCHIS- CCBs was performed with NaOH solution. The biosorption capacity of phenol of 28.4 mg/g on the SCHIS- CCBs at pH 2.0 and 313 K, determined by the Langmuir equation, indicated the promising potential of such modified chitosan biosorbents.

Efficient removal of methylene blue dye by a hybrid adsorption– photocatalysis process using reduced graphene oxide/titanate nanotube composites for water reuse

Chi Hieu Nguyen,Ruey-Shin Juang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.76 No.-

In this study, reduced graphene oxide/titanate nanotube (rGO/TNT) composites were fabricated by a one-step facile hydrothermal process. The structures and physicochemical properties of the prepared materials were comprehensively determined. The incorporation of a small amount of rGO into the composites led to the enhancement of the absorption intensity of visible light and the separation of photogenerated charged carriers. The rGO/TNT composite with the optimal amount of rGO of 3% by weight showed the highest photocatalytic activity for both methylene blue (MB) and methyl orange. Moreover, 3 wt.% rGO/TNT exhibited higher adsorption capability and photocatalytic activity for MB, a cationic dye, than TNTs and commercial TiO2 P25. The maximum amount of MB adsorbed on 3 wt.% rGO/TNT was 26.3 mg/g at 25 °C, and the adsorption rapidly reached equilibrium after 40 min of contact time. Approximately 100% decolorization and 77.4% mineralization over 3% rGO/TNT composite were achieved under 100 W UV irradiation for 1 h, whereas 95.0% decolorization and 78.8% mineralization were achieved under visible light irradiation for 3 h. The degradation pathways of MB over P25, TNTs, and 3 wt.% rGO/TNT composite were finally proposed and compared.

Hydrothermal synthesis of cobalt germanium oxide supported with carbon-based graphitic carbon nitride for electrochemical determination of ornidazole

Jaysiva Ganesamurthi,Xiang-Ren Chen,Ruey-Shin Juang,Siang-Yu Wei,Da-Yuan Lin 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-

In recent decades, nitroimidazole derivatives have played an effectual role against bacterial and protozoaninfections that can be used as anti-cancer and antibiotic drugs. Ornidazole (ORD) consists of a major5-imidazole nucleus which complements to first nitro groups, ORD invades into lipid tissue and othernitroimidazole derivatives. The periodic intake of ORD orally causes side effects of headache, nausea,vomiting, and breathing difficulties such effects need potential monitoring and develop essential electrochemicalsensing toward ORD. In this study, we developed a ternary cobalt germanium oxide (Co2GeO4)nanoparticle prepared by a facile hydrothermal method and followed by calcination. The carbon-basedgraphitic carbon nitride (GCN, g-C3N4) nanosheets were therefore supported for electrochemical sensingapplications in order to improve the features like the catalytic activity, electrical conductivity, and activesurface area of Co2GeO4 composites. The structural, chemical composition, and morphological propertiesof samples were characterized. Subsequently, the fabrication of cobalt germanium oxide with graphiticcarbon nitride (Co2GeO4/GCN) was modified over a screen-printed carbon electrode (SPCE) towards electrochemicaldetection of ORD. Electrochemical measurements were recorded by cyclic voltammetry (CV)and differential pulse voltammetry (DPV) for the determination of ORD. The Co2GeO4/GCN nanocompositeexhibits a wide linear range response of 0.049–94.9 lM in DPV with a nanomolar limit of detection of7.2 nM of ORD and a high sensitivity of 2.66 lA lM1 cm2. An interference study was carried out for theCo2GeO4/GCN nanocomposite in the existence of hazardous metals, biological compounds, and similardrugs for examining the selectivity of the sensor. The proposed Co2GeO4/GCN nanocomposite revealssuperior sensing selectivity towards ORD; hence, repeatability, reproducibility, and storage stability wererecorded in their RSD and found to be less than 0.4%, adding to this practical feasibility of the sensor analyzedby real samples of blood serum & human urine with exceptional recovery percentages.

Preparation of polyaminated Fe3O4@chitosan core-shell magnetic nanoparticles for efficient adsorption of phosphate in aqueous solutions

Chun-Chieh Fu,Hai Nguyen Tran,Xing-Han Chen,Ruey-Shin Juang 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.83 No.-

In this study, novel polyethylenimine (PEI)-grafted chitosan (CS) core-shell (called as Fe3O4/CS/PEI)magnetic nanoparticles were synthesized and applied for adsorbing phosphate in water. The magneticand physicochemical properties of as-synthesized nanoparticles werefirst analyzed by means of asuperconducting quantum interference device, X-ray diffractometer, X-ray photoelectron spectroscope,Fourier-transform infrared spectroscope, transmission electron microscope, zeta potential analyzer, andnitrogen sorptiometer. The followed experiments indicated that the amount of phosphate adsorbedincreased with increasing equilibrium pH to near 4.0 and then decreased when the pH was furtherincreased. The adsorption isotherms of phosphate on Fe3O4/CS/PEI particles were wellfit by the Langmuirequation, and its maximum adsorption was 48.2 mg g 1 at an equilibrium pH of 3.0 and 25 C. Theprimary mechanism for phosphate adsorption on Fe3O4/CS/PEI particles was electrostatic attraction. Thepreferential adsorption of phosphate in the presence of other excess anions including chloride, nitrate,carbonate, and sulfate was presented. Under the conditions studied, more than 90% of phosphate wasdesorbed from the laden Fe3O4/CS/PEI particles by 0.05 mol L 1 of NaOH solution. The remainedadsorption efficiency obtained in thefive-cycle reusability tests has demonstrated the promisingapplication potential of Fe3O4/CS/PEI particles.

Direct Purification of Burkholderia Pseudomallei Lipase from Fermentation Broth Using Aqueous Two-Phase Systems

Chien Wei Ooi,Beng Ti Tey,Siew Ling Hii,Arbakariya Ariff,Ho Shing Wu,John Chi Wei Lan,Ruey Shin Juang,Siti Mazlina Mustapa Kamal,Tau Chuan Ling 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.6

An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study An aqueous two-phase purification process was employed for the recovery of Burkholderia pseudomallei from fermentation broth. The partition behavior of B.pseudmallei lipase was investigated with various parameters such as phase composition, tie-line length (TLL), volume ratio (VR), sample loading, system pH, and addition of neutral salts. Optimum conditions for the purification of lipase were obtained in polyethylene glycol (PEG) 6000-potassium phosphate system using TLL of 42.2% (w/w), with VR of 2.70, and 1% (w/w) NaCl addition at pH 7 for 20% (w/w) crude load. Based on this system, the purification factor of lipase was enhanced to 12.42 fold, with a high yield of 93%. Hence, the simplicity and effectiveness of aqueous two-phase systems (ATPS) in the purification of lipase were proven in this study