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Ruey Shin Juang,Chun-Lan Yeh 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
The use of macroporous polymeric adsorptionresins for the recovery and purification of prodigiosin fromfermentation broth of Serratia marcescens SMDR wassystematically studied, in which the broth was pretreatedby coagulation with alum and the resulting precipitate wasleached by methanol/water solution. Of the seven resinstested, Diaion HP-20 resin was selected considering theadsorption and desorption abilities for prodigiosin at thesame time. The optimal compositions of liquid phases foradsorption and desorption were also examined. Batchexperiments were performed at 15 ~ 35oC by varying initialprodigiosin concentration in solution (0.05 ~ 0.5 mmol/L),in which molar fraction of each component in the solutionwas kept constant. The Freundlich and Langmuir equationswere used to describe adsorption isotherms and the relatedthermodynamic functions were also determined. Fixed-bedexperiments were finally conducted to obtain the breakthroughcharacteristics for the adsorption and desorption ofprodigiosin. Under optimized conditions, a purificationfactor of prodigiosin of 11.4 could be obtained from thepretreated broth after one adsorption-desorption run in fixedbed. The present results had demonstrated the promisingpotential of HP-20 resins for the recovery and purificationof prodigiosin from methanol/water solution of Serratiamarcescens fermentation broth.