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Qiu, Zhen-Qin,Qiu, Zhen-Rong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8
Objective: To explore the sensitivity of gastric cancer cells to chemotherapy drugs in elderly patients and its correlation with cyclooxygenase-2 (COX-2) expression in cancer tissue. Materials and Methods: Forty-three elderly patients with gastric cancer (observation group) and 31 young patients with gastrointestinal tumors (control group) who were all diagnosed by pathology and underwent surgery in the 89th Hospital of Chinese People's Liberation Army were selected. Drug sensitivity testing of tumor cells in primary culture was carried out in both groups using a methyl thiazolyl tetrazolium (MTT) method, and the expression of COX-2 and the factors related to multi-drug resistance (MDR) in cancer tissue were assessed by immunohistochemistry. Results: The inhibition rates (IR) of vincristine (VCR), 5-fluorouracil (5-FU), oxaliplatin (L-OHP), mitomycin (MMC) and epirubicin (eADM) on tumor cells in the observation group were dramatically lower than in the control group, with statistical significance (P<0.05 or P<0.01). The positive rates of COX-2, glutathione s-transferase-${\pi}$ (GST-${\pi}$) and P glycoprotein (P-gp) expression in cancer tissue in the observation group were all higher than in control group (P<0.05), while that of DNA topoisomerase $II{\alpha}$ ($TopoII{\alpha}$) expression lower than in the control group (P<0.01). In the observation group, COX-2 expression in cancer tissue had a significantly-positive correlation with GST-${\pi}$ and P-gp (r=0.855, P=0.000; r=0.240, P=0.026), but a negative correlation with $TopoII{\alpha}$ (r=-0.328, P=0.002). In the control group, COX-2 expression in cancer tissue was only correlated with P-gp positively (r=0.320, P=0.011). Bivariate correlation analysis displayed that COX-2 expression in cancer tissue in the observation group had a significantly-negative correlation with the IRs of 5-FU, L-OHP, paclitaxel (PTX) and eADM in tumor cells (r=-0.723, P=0.000; r=-0.570, P=0.000; r=-0.919, P=0.000; r=-0.781, P=0.000), but with hydroxycamptothecine (HCPT), VCR and 5-FU in the control group (r=-0.915, P=0.000; r=-0.890, P=0.000; r=-0.949, P=0.000). Conclusions: Gastric cancer cells in elderly patients feature stronger MDR, which may be related to high COX-2 expression.
Rong Jie Zha,Zheng Lin Zhao,Xiu Feng Zhao,Guang Wen Zha,Meng Quan Li,Yi Yan Wu,Jing Qiu Li,Li Xin Guan,김상찬 대한한의학방제학회 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.
Li, Qiu-Wen,Lu, Can-Rong,Ye, Ming,Xiao, Wen-Hua,Liang, Jun Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1
We conducted a case-control study in China to clarify the association between XRCC1-Arg399Gln polymorphism and HCC risk. A total of 150 cases and 158 controls were selected from the the Affiliated Hospital of Qingdao University from May 2008 to May 2010. XRCC1-Arg399Gln polymorphism was based upon duplex polymerase-chain-reaction with the confronting-two-pairprimer (PCR-CTPP) method. All analyses were performed using the STATA statistical package. A significantly increased risk was associated with the Arg/Gln genotype (adjusted OR 1.78, 95%CI=1.13-2.79) compared with genotype Arg/Arg. In contrast, the Gln/Gln genotype had non-significant increased risk of HCC with adjusted OR (95%CI) of 1.69 (0.93-2.66). A significant association was found between positive HBsAg and Arg/Gln, with an OR of 3.43 (95% CI=1.45-8.13). Patients carrying Gln/Gln genotypes showed significantly lower median survival than Arg/Arg genotypes (HR=1.38, 95% CI=1.04-1.84). Further Kaplan-Meier analysis showed decreased median survival in Arg/Gln+Gln/Gln genotype carriers in comparison to Arg/Arg carriers (HR=1.33, 95% CI=1.02-1.76). In conclusion, we observed that XRCC1-Arg399Cln polymorphism is associated with susceptibility to HCC, and XRCC1 Gln allele genotype showed significant prognostic associations.
An Epigenetic Mechanism Underlying Doxorubicin Induced EMT in the Human BGC-823 Gastric Cancer Cell
Han, Rong-Fei,Ji, Xiang,Dong, Xing-Gao,Xiao, Rui-Jing,Liu, Yan-Ping,Xiong, Jie,Zhang, Qiu-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
The epithelial to mesenchymal transition (EMT) is a key step during embryonic morphogenesis and plays an important role in drug resistance and metastasis in diverse solid tumors. We previously reported that 48 h treatment of anti-cancer drug doxorubicin could induce EMT in human gastric cancer BGC-823 cells. However, the long term effects of this transient drug treatment were unknown. In this study we found that after 48 h treatment with $0.1{\mu}g/ml$ doxorubicin, most cells died during next week, while a minor population of cells survived and formed colonies. We propagated the surviving cells in drug free medium and found that these long term cultured drug survival cells (abbreviated as ltDSCs) retained a mesenchymal-like cell morphology, and expressed high levels of EMT-related molecules such as vimentin, twist and ${\beta}$-catenin. The expression of chromatin reprogramming factors, Oct4 and c-myc, were also higher in ltDSCs than parental cells. We further demonstrated that the protein level of p300 was upregulated in ltDSCs, and inhibition of p300 by siRNA suppressed the expression of vimentin. Moreover, the ltDSCs had higher colony forming ability and were more drug resistant when compared to parental cells. Our results suggested that an epigenetic mechanism is involved in the EMT of ltDSCs.
Xinshan Rong,Fengxian Qiu,Jiao Qin,Jie Yan,Hao Zhao,Dongya Yang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5
A novel water-soluble adsorbent (S-Me/MA) was prepared from melamine and maleic anhydride by two steps. The structure of material was characterized by FT-IR spectroscopy, SEM, and 1H NMR. The S-Me/MA was applied to deal with wastewater solution containing malachite green (MG). The influences of adsorbent dose, temperature, pH and contact time were investigated. At the same time, the adsorption kinetic and isotherm of S-Me/MA toward malachite green were investigated. The results indicated that the adsorption of malachite green was in line with pseudo-second order kinetics equation. Langmuir isotherm equation can be a fine description of the removal process.
Xinshan Rong,Fengxian Qiu,Jiao Qin,Hao Zhao,Jie Yan,Dongya Yang 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.26 No.-
A series of NiO/graphene nanosheets adsorbents (NGNS) were synthesized by a facile hydrothermal method, and characterized by UV–vis, XRD, TG, TEM, HRTEM, Raman, SAED and XPS methods. The NGNS was applied to deal with wastewater solution containing Congo Red (CR); and adsorption efficiency attained 99.56% at optimum condition. The adsorption kinetic and isotherm of adsorbent toward CR were investigated. The results indicated that pseudo second-order kinetic model and the equilibrium data were well fitted by Redlich–Peterson model. The adsorbent can be separated from system effectively and easily. The prepared adsorbent has potential application for the wastewater treatment containing CR azo-dye.
Yu-rong Deng,Qiu-li Wu,Wei Zhang,Ze-cheng Lv 보안공학연구지원센터 2015 International Journal of Grid and Distributed Comp Vol.8 No.5
Aiming at the provincial level within the grid wide-area distributed substation equipment on-line monitoring various types, processing of large amount of data and communication standard is not unified. Optimization of the structure of communication between the various levels, researched open communication format specification, established data interaction integration model. The paper studied the wide-area distributed on-line monitoring comprehensive processing unit of end stations with provincial level network of on-line monitoring system for the main platform of communication technology. Application shows that systems runs stability and meet online monitoring data collection, processing, transmission, and other functions, and has good maintainability and extensibility, adapt to various on-line monitoring in province level the development trend of the main platform for centralized management.
Zhao, Rong Jie,Zhao, Zheng Lin,Zhao, Xiu Feng,Zhao, Guang Wen,Li, Meng Quan,Wu, Yi Yan,Li, Jing Qiu,Guan, Li Xin,Kim, Sang-Chan The Korean Medicine Society for the Herbal Formula 2009 大韓韓醫學方劑學會誌 Vol.17 No.2
The effects of aqueous extract of Schizandrae Fructus (AESC) on lead (Pb)-induced changes of monoamine neurotransmitters in the hippocampus (HIP) of adult rats were investigated. Male Sprague-Dawley rats were received intraperitoneal (i.p.) administration of Pb acetate (5 mg/kg/d) for 28 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in HIP were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Pb treated rats (P < 0.05), while pretreatment with AESC (100 mg/kg/d or 300 mg/kg/d, p.o., 2 h before Pb) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC (300 mg/kg/d) significantly increased the reduction of glutathione contents and superoxide dismutase activities in HIP induced by chronic Pb. These results suggest that AESC ameliorates Pb-induced depletion of monoamine neurotransmitters in HIP through its antioxidant activity.