The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage

Reza Shiri,Fatemeh Yari,Minoo Ahmadinejad,Shahram Vaeli,Mohammad Reza Tabatabaei 대한혈액학회 2014 Blood Research Vol.49 No.1

Background Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of platelets decreased, storage lesions were observed, and cells underwent apoptosis. We investigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage. Methods Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22℃ for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were analyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay. Background Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of platelets decreased, storage lesions were observed, and cells underwent apoptosis. We investigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage. Methods Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22℃ for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were analyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay. Results At day 4 of storage, ristocetin-induced platelet aggregation was significantly higher in the inhibitor-treated (test) than in control samples; the difference was not significant at day 7. There was no significant difference in arachidonic acid-induced platelet aggregation between test and control samples. However, at day 7 of storage, the binding of platelets to vWF was significantly higher in test than in control samples. The MTT assay revealed significantly higher viability in test than in control samples at both days of study. Conclusion Treatment of platelets with caspase-3 inhibitor could increase their functionality and survival.

The caspase-3 inhibitor (peptide Z-DEVD-FMK) affects the survival and function of platelets in platelet concentrate during storage

Reza Shiri,Fatemeh Yari,Minoo Ahmadinejad,Shahram Vaeli,Mohammad Reza Tabatabaei 대한혈액학회 2014 Blood Research Vol.49 No.1

Background Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of platelets decreased, storage lesions were observed, and cells underwent apoptosis. We investigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage. Methods Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22℃ for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were analyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay. Background Although apoptosis occurs in nucleated cells, studies show that this event also occurs in some anucleated cells such as platelets. During storage of platelets, the viability of platelets decreased, storage lesions were observed, and cells underwent apoptosis. We investigated the effects of caspase-3 inhibitor on the survival and function of platelets after different periods of storage. Methods Platelet concentrates were obtained from the Iranian Blood Transfusion Organization in plastic blood bags. Caspase-3 inhibitor (Z-DEVD-FMK) was added to the bags. These bags along with control bags to which no inhibitor was added were stored in a shaking incubator at 22℃ for 7 days. The effects of Z-DEVD-FMK on the functionality of platelets were analyzed by assessing their ability to bind to von Willebrand factor (vWF) and to aggregate in the presence of arachidonic acid and ristocetin. Cell survival was surveyed by MTT assay. Results At day 4 of storage, ristocetin-induced platelet aggregation was significantly higher in the inhibitor-treated (test) than in control samples; the difference was not significant at day 7. There was no significant difference in arachidonic acid-induced platelet aggregation between test and control samples. However, at day 7 of storage, the binding of platelets to vWF was significantly higher in test than in control samples. The MTT assay revealed significantly higher viability in test than in control samples at both days of study. Conclusion Treatment of platelets with caspase-3 inhibitor could increase their functionality and survival.

A Teleoperation System for Micro Positioning with Haptic Feedback

Reza Seifabadi,Seyed Mehdi Rezaei,Saeed Shiry Ghidary,Mohammad Zareinejad 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.4

This paper presents the research work on a 1 Degree of Freedom (DOF) macro-micro teleoperation system which enables human operator to perform complex task in micro environment such as cell insertion with the capability of haptic feedback. To reach submicron resolution, a nano-motion piezo actuator was used as the slave robot and a servo DC motor was used as the master robot. Force sensors were implemented at both ends for haptic feedback and a microscope equipped with camera was employed for real-time visual feedback. The hysteresis nonlinearity of the piezo motor was modeled using LuGre friction model and compensated for. A Sliding Mode Based Impedance Controller (SMBIC) was designed at the slave side to ensure position tracking while an impedance force controller was designed at the master side to ascertain tracking of the force. Control parameters were chosen based on Llewellyn stability criteria such that the entire system stays stable against parameter uncertainties and constant time delay. The experimental results demonstrated capability of the proposed control frameworks in desirable tracking of the position and force signals while the entire system remained stable. The results of this study can be used for complex tasks in micron environment such as cell insertion.

Silica nanoparticles modified with a Schiff base ligand: An efficient adsorbent for Th(IV), U(VI) and Eu(III) ions

Mohammad Reza Yaftian,Zahra Shiri-Yekta,Abdolreza Nilchi 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.8

Modification of SiO2 nanoparticles by salicylaldiminepropyl results in efficient adsorbents for removal of Th4+, UO22+ and Eu3+ ions from aqueous solutions. The effect of parameters influencing the adsorption efficiency such as aqueous phase pH, contact time, initial metal ions concentration, adsorbent dosage and temperature dependency of the process was verified and discussed. Under optimal conditions (pH 5.5, adsorbent dosage 0.05 g, contact time 30 min. and 25 oC), thorium and uranyl ions (initial concentration 20 mg/l) were quantitatively removed from 20 ml of sample solution. Under such conditions 85% of europium ions was removed. Comparison of the adsorption efficiency of the studied modified nano-particles with those unmodified ones shows a shift for uptake of the metal ions vs. pH curves towards lower pH values by applying the modified adsorbents. In addition, a significant improvement of europium ions adsorption was observed by using the modified nanoparticles. Kinetics of the process was studied by considering a pseudo second-order model. This model predicts chemisorption for the adsorption mechanism. Freundlich, Langmuir and Temkin models were suitable for describing the equilibrium data of Th4+, UO22+ and Eu3+ adsorption process, respectively. Thermodynamic investigation reveals the adsorption process of the studied ions is entropy driven.

Synthesis, Characterization and Structure of DBU-hydrobromide-perbromide: A Novel Oxidizing Agent for Selective Oxidation of Alcohols to Carbonyl Compounds

Mehdi Bakavoli,Mohammad Rahimizadeh,Hossein Eshghi,Ali Shiri,Zahra Ebrahimpour,Reza Takjoo 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.4

A new and efficient reagent for the conversion of primary and secondary alcohols into their corresponding aldehydes and ketones is introduced. The reagent was easily prepared from the reaction of DBU with molecular bromine in CHCl3. The structure of the reagent as DBUH+Br3– was determined by single crystal X-ray diffraction analysis.

Synthesis, Characterization and Structure of DBU-hydrobromide-perbromide: A Novel Oxidizing Agent for Selective Oxidation of Alcohols to Carbonyl Compounds

Bakavoli, Mehdi,Rahimizadeh, Mohammad,Eshghi, Hossein,Shiri, Ali,Ebrahimpour, Zahra,Takjoo, Reza Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.4

A new and efficient reagent for the conversion of primary and secondary alcohols into their corresponding aldehydes and ketones is introduced. The reagent was easily prepared from the reaction of DBU with molecular bromine in $CHCl_3$. The structure of the reagent as $DBUH^+{Br_3}^-$ was determined by single crystal X-ray diffraction analysis.

An experimental study on the mechanisms of enhancing oil recovery by nanoparticles-assisted surfactant flood

Sina Mobaraki,Mahdi Zakavi,Omid Mahmoodi,Milad Omidvar Sorkhabadi,Seyyed Shahram Khalilinezhad,Reza Shiri Torkmani 한국자원공학회 2020 Geosystem engineering Vol.23 No.6

The central concern of this study is to examine the synergic effects of an anionic surfactant and hydrophilic silica nanoparticles on the improvement of surfactant properties. The assessment of the effect of silica nanoparticles on the emulsification ability of the surfactant solution was performed by the use of both electrical conductivity measurements and phase behavior tests. Accordingly, an optimal chemical formulation for the utilized crude oil was suggested. Optical absorption technique was also employed to evaluate the stability of the optimal solution. The oil recovery experiments were carried out in a transparent glass micromodel saturated with crude oil to monitor some pore-scale displacement events during the simultaneous flow of the optimal solution and the crude oil through porous media. Phase behavior tests indicated that the silica nanoparticles are effective in terms of Interfacial tension (IFT) reduction since they can achieve ultra-low IFT level. The results of conductivity experiments proved the ability of silica nanoparticles to reduce the Critical Micelle Concentration (CMC) of the surfactant. The stability tests proved that the optimal solution is stable for a reasonable time. The multiphase flow experiments revealed that oil recovery increased by about 5% during nanoparticles-assisted surfactant flooding compared with surfactant flood.