Effect of Dietary Feeding Regimens on Urea and Protein Concentration of Milk in Murrah Buffaloes

B. Roy,R. K. Mehla,S. K. Sirohi 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

The present study was planned to examine the effect of different feeding regimens on milk urea concentration and milk protein concentration. The objectives are to describe the diurnal variations of milk urea (MU) concentration and to predict plasma urea (PU) concentration from MU concentration. Six lactating Murrah buffaloes were distributed in two groups and were fed two different diets in a crossover design. The diets consisted of leguminous crops as diet 1 (berseem (Trifolium alexandrinum)+concentrate mixture 1+wheat straw)) and non-leguminous crops as diet 2 (oats (Avana sativa)+concentrate mixture 2+wheat straw). All the diets were isocaloric and isonitrogenous. Each diet was fed to the animals for a period of 28 days, followed by a 10 day gap to obviate the carry over effect of the previous diet and then a switch over to the other diet. Digestibility trials were conducted on the last 7 days of each feeding period. Milk samples were collected on day 3, 7, 10, 14, 17, 21, 24 and 28 of the feeding period and blood samples were collected on the same day at morning within 30 minutes after morning milking. The average milk urea (MU) values (mg/dl) differed significantly (p<0.01) and were 44.83짹0.62 and 42.53짹0.73, respectively, for diets 1 and 2. Milk urea concentrations (mg/dl) also varied (p 0.01) among the days of feeding period, but were stabilized after 10th day of feeding period. In contrast, diets and days of feeding period had no significant effect on percent milk protein. Plasma urea concentration showed a significant (p<0.01) positive correlation (r = 0.93) with MU concentration. To predict the PU from MU the following equation was developed `PU = 10.67짹0.76횞MU (mg/dl) with R2 = 0.87`. A clear diurnal variation of MU was found with lowered morning value (42.04짹0.68 mg/dl) than the evening value (45.32짹0.66 mg/dl). Present findings suggested that MU or PU concentration could be used as an indicator to monitor the feeding strategy. Plasma urea can be predicted from MU, whenever interpretation of milk urea data required consideration of diurnal variation.

EFFECTS OF VARYING LEVELS OF DIETARY PROTEIN ON THE PERFORMANCE AND PRODUCTION COST OF WHITE PEKIN DUCKLINGS

Roy, D.R.,Ali, M.A.,Chowdhury, S.D. Asian Australasian Association of Animal Productio 1994 Animal Bioscience Vol.7 No.2

An experiment was conducted to investigate the performance and production cost of Pekin ducklings fed varying levels of dietary protein. One hundred and twenty ducklings in four separate groups received either 16, 18, 20 or 22% CP in the starter period (day-old to 3rd week of age). These four groups ate either 12, 14, 16 or 18% CP respectively during the grower-finisher period (4th week to 12th week of age). The results of the study indicated that in a tropical country like Bangladesh, the protein needs of Pekin ducklings should be satisfied depending on the age at which the birds are to be marketed. If the ducklings are to be marketed at 6 weeks, 22/18% CP would be sufficient. When the marketing is aimed at 9 weeks of age, 20/16% would be adequate. For ducklings which are to be marketed at 12 weeks of age, a protein level of 18/14% would be required. All these results should help the producers to keep production cost to a minimum. The exceptional capacity of ducklings for compensatory growth would help keep birds on low protein levels if they are reared upto 9 or 12 weeks before marketing.

Effect of Dietary Feeding Regimens on Urea and Protein Concentration of Milk in Murrah Buffaloes

Roy, B.,Mehla, R.K.,Sirohi, S.K. Asian Australasian Association of Animal Productio 2005 Animal Bioscience Vol.18 No.7

The present study was planned to examine the effect of different feeding regimens on milk urea concentration and milk protein concentration. The objectives are to describe the diurnal variations of milk urea (MU) concentration and to predict plasma urea (PU) concentration from MU concentration. Six lactating Murrah buffaloes were distributed in two groups and were fed two different diets in a crossover design. The diets consisted of leguminous crops as diet 1 (berseem (Trifolium alexandrinum)+concentrate mixture 1+wheat straw)) and non-leguminous crops as diet 2 (oats (Avana sativa)+concentrate mixture 2+wheat straw). All the diets were isocaloric and isonitrogenous. Each diet was fed to the animals for a period of 28 days, followed by a 10 day gap to obviate the carry over effect of the previous diet and then a switch over to the other diet. Digestibility trials were conducted on the last 7 days of each feeding period. Milk samples were collected on day 3, 7, 10, 14, 17, 21, 24 and 28 of the feeding period and blood samples were collected on the same day at morning within 30 minutes after morning milking. The average milk urea (MU) values (mg/dl) differed significantly (p<0.01) and were 44.83${\pm}$0.62 and 42.53${\pm}$0.73, respectively, for diets 1 and 2. Milk urea concentrations (mg/dl) also varied (p 0.01) among the days of feeding period, but were stabilized after 10th day of feeding period. In contrast, diets and days of feeding period had no significant effect on percent milk protein. Plasma urea concentration showed a significant (p<0.01) positive correlation (r = 0.93) with MU concentration. To predict the PU from MU the following equation was developed 'PU = 10.67${\pm}$0.76${\times}$MU (mg/dl) with $R^2$ = 0.87'. A clear diurnal variation of MU was found with lowered morning value (42.04${\pm}$0.68 mg/dl) than the evening value (45.32${\pm}$0.66 mg/dl). Present findings suggested that MU or PU concentration could be used as an indicator to monitor the feeding strategy. Plasma urea can be predicted from MU, whenever interpretation of milk urea data required consideration of diurnal variation.

Effect of hemocompatibility on the surface properties of Si incorporated diamond like carbon films

R.K. Roy,S.J. Park,K.R. Lee,D.K. Han,J.J. Shin 한국표면공학회 2006 한국표면공학회 학술발표회 초록집 Vol.2006 No.-

Compositional homogeneity and X-ray topographic analyses of CdTe_<i>x</i>Se_1−<i>x</i> grown by the vertical Bridgman technique

Roy, U.N.,Bolotnikov, A.E.,Camarda, G.S.,Cui, Y.,Hossain, A.,Lee, K.,Lee, W.,Tappero, R.,Yang, Ge,Cui, Y.,Burger, A.,James, R.B. Elsevier 2015 Journal of crystal growth Vol.411 No.-

<P><B>Abstract</B></P> <P>We grew CdTe<SUB> <I>x</I> </SUB>Se<SUB>1−<I>x</I> </SUB> crystals with nominal Se concentrations of 5%, 7%, and 10% by the vertical Bridgman technique, and evaluated their compositional homogeneity and structural quality at the NSLS’ X-ray fluorescence and white beam X-ray topography beam lines. Both X-ray fluorescence and photoluminescence mapping revealed very high compositional homogeneity of the CdTe<SUB> <I>x</I> </SUB>Se<SUB>1−<I>x</I> </SUB> crystals. We noted that those crystals with higher concentrations of Se were more prone to twinning than those with a lower content. The crystals were fairly free from strains and contained low concentrations of sub-grain boundaries and their networks.</P> <P><B>Highlights</B></P> <P> <UL> <LI> CdTeSe crystals were grown by the vertical Bridgman technique. </LI> <LI> Very high compositional homogeneity. </LI> <LI> Low concentration of sub-grain boundaries. </LI> <LI> Almost free from sub-grain boundary network. </LI> </UL> </P>

Absence of P53 Gene Mutations in Exons 5 - 7 Among Breast Cancer Patients of Bengalee Hindu Caste Females, West Bengal, India

Roy, Abhishikta Ghosh,Sarkar, B.N.,Roy, Rakesh,Rao, V.R.,Bandyopadhyay, A.R. Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Background: The high incidence and relatively good prognosis of breast cancer has made it the most prevalent cancer in the world today. A large number of distinct mutations and polymorphisms in the p53 gene have been reported worldwide, but there is no report regarding the role of this inherited susceptibility gene in breast cancer risk among the Bengalee Hindu Caste females of West Bengal, India. Aim of the Study: We investigated the distribution and the nature of p53 gene mutations and polymorphisms in exons 5-7 in a cohort of 110 Bengalee Hindu breast cancer patients and 127 age, sex and caste matched controls by direct sequencing. Results: We did not observe any mutations and polymorphisms in our studied individuals. Conclusion: We therefore conclude that mutations in exons 5-7 of p53 gene are rare causes of breast cancer among Bengalee Hindu caste females, and therefore of little help for genetic counseling and diagnostic purposes.

Crystal Growth, Spectral, Magnetic, Antibacterial and Antifungal Studies of Co(II) and Ni(II) Complexes of 4-Nitrobenzoic Acid

Roy, Sunalya M.,Sudarsanakumar, M.R.,Dhanya, V.S.,Suma, S.,Kurup, M.R. Prathapachandra Korean Chemical Society 2014 대한화학회지 Vol.58 No.3

Single crystals of cobalt(II) and nickel(II) complexes of 4-nitrobenzoic acid have been successfully grown by gel diffusion technique. The grown crystals were characterized by elemental analysis, FT-IR and UV-Visible spectroscopy. The structure of the grown crystals was elucidated using single crystal X-ray diffraction studies. The title compounds $[Co(Pnba)_2(H_2O)_4]{\cdot}2H_2O$ 1 and $[Ni(Pnba)_2(H_2O)_4]{\cdot}2H_2O$ 2 where PnbaH=4-nitrobenzoic acid, crystallize in centrosymmetric triclinic space group P-1. Magnetic susceptibility measurements reveal that the compounds are paramagnetic in nature. The mechanical strength of the grown crystals was determined by Vicker's microhardness studies. The ligand (4-nitrobenzoic acid) and the complexes have been screened for their biological activity against various bacteria and fungi. The activity data show that the biological activity of the complexes is higher than that of the ligand.

Sequence Analysis and Potential Action of Eukaryotic Type Protein Kinase from Streptomyces coelicolor A3(2)

Roy, Daisy R.,Chandra, Sathees B.C. Korea Genome Organization 2008 Genomics & informatics Vol.6 No.1

Protein kinase C (PKC) is a family of kinases involved in the transduction of cellular signals that promote lipid hydrolysis. PKC plays a pivotal role in mediating cellular responses to extracellular stimuli involved in proliferation, differentiation and apoptosis. Comparative analysis of the PKC-${\alpha},{\beta},{\varepsilon}$ isozymes of 200 recently sequenced microbial genomes was carried out using variety of bioinformatics tools. Diversity and evolution of PKC was determined by sequence alignment. The ser/thr protein kinases of Streptomyces coelicolor A3 (2), is the only bacteria to show sequence alignment score greater than 30% with all the three PKC isotypes in the sequence alignment. S.coelicolor is the subject of our interest because it is notable for the production of pharmaceutically useful compounds including anti-tumor agents, immunosupressants and over two-thirds of all natural antibiotics currently available. The comparative analysis of three human isotypes of PKC and Serine/threonine protein kinase of S.coelicolor was carried out and possible mechanism of action of PKC was derived. Our analysis indicates that Serine/ threonine protein kinase from S. coelicolor can be a good candidate for potent anti-tumor agent. The presence of three representative isotypes of the PKC super family in this organism helps us to understand the mechanism of PKC from evolutionary perspective.

Seed and Root Rots of Ginseng (Panax quinquefolius L) Caused by Cylindrocarpon destructans and Fusarium spp.

Reeleder, R.D.,Roy, R.,Capell, B. The Korean Society of Ginseng 2002 Journal of Ginseng Research Vol.26 No.3

Ginseng (Panax quinquefolius L.) has become one of the most valuable herb crops grown in North America. However, traditional cropping practices are favourable to disease and significant losses due to root disease are common, despite frequent use of fungicides. Seedlots are often contaminated with pathogens, however, little is known about the causes of seed decay and the role of seed pathogens as incitants of root rots. It was shown that both Fusarium spp. and Cylindrocarpon destructans were able to rot seeds and that C. destructans was more virulent than Fusarium spp. on seedling roots. A modified rose bengal agar MRBA) medium (1 g KH$_2$PO$_4$; 0.5 g MgSO$_4$; 50 mg rose bengal; 10 g dextrose; 5 g Bacto peptone; 15 g Bacto agar; 30 mg streptomycin sulfate; 250 mg ampicillin; 10 mg rifampicin; 500mg pentachloronitrobenzene; 500 mg dicloran; and 1 L distilled water) was superior to potato dextrose agar in detecting C. destuctans in diseased roots. Isolation of C. destructans from diseased seedlings arising from seeds sown in replant soil supported the hypothesis that this pathogen is a cause of ginseng replant failure in North America.

Sequence Analysis and Potential Action of Eukaryotic Type Protein Kinase from Streptomyces Coelicolor A3(2)

Daisy R. Roy,Sathees B.C.Chandra 한국유전체학회 2008 Genomics & informatics Vol.6 No.1

Protein kinase C (PKC) is a family of kinases involved in the transduction of cellular signals that promote lipid hydrolysis. PKC plays a pivotal role in mediating cellular responses to extracellular stimuli involved in proliferation, differentiation and apoptosis. Comparative analysis of the PKC-α,β,ε isozymes of 200 recently sequenced microbial genomes was carried out using variety of bioinformatics tools. Diversity and evolution of PKC was determined by sequence alignment. The ser/thr protein kinases of Streptomyces coelicolor A3 (2) , is the only bacteria to show sequence alignment score greater than 30% with all the three PKC isotypes in the sequence alignment. S.coelicolor is the subject of our interest because it is notable for the production of pharmaceutically useful compounds including anti-tumor agents, immunosupressants and over two-thirds of all natural antibiotics currently available. The comparative analysis of three human isotypes of PKC and Serine/ threonine protein kinase of S.coelicolor was carried out and possible mechanism of action of PKC was derived. Our analysis indicates that Serine/ threonine protein kinase from S. coelicolor can be a good candidate for potent anti-tumor agent. The presence of three representative isotypes of the PKC super family in this organism helps us to understand the mechanism of PKC from evolutionary perspective.