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      • Inhibition of Eimeria tenella Development in vitro Mediated by Chicken Macrophages and Fibroblasts Treated with Chicken Cell Supernatants with IFN-g Activity

        Dimier, Isabelle H.,Quere, Pascale,Naciri, Muriel,Bout, Daniel T. JSTOR 1998 Avian diseases Vol.42 No.2

        <P>Pretreatment of chicken macrophages or fibroblasts with supernatants from concanavalin A-stimulated spleen cells or from the virus-transformed cell line reticuloen-dotheliovirus as source of interferon gamma (IFN-&ggr;) slows down subsequent sporozoite replication in the cells. To identify the presence of IFN-&ggr;, we combined four typical activities of IFN-&ggr;: inhibition of cytopathic effect of vesicular stomatitis virus on IFN-&ggr;-treated fibroblasts, cytostatic activity of IFN-&ggr;-activated macrophages, induction of major histocompatibility complex II antigen expression on IFN-&ggr;-activated fibroblasts and macrophages, and induction of nitrite production in macrophages. We have shown that chicken fibroblasts and macrophages possess a microbiostatic capacity once they are able to prevent the otherwise unchecked intracellular replication of Eimeria tenella following activation with culture supernatants identified as containing a strong IFN-&ggr; activity. /// El pretratamiento de macrófagos aviares o de fibroblastos con sobrenadantes de células esplénicas estimuladas con concalabalina A o de una linea celular transformada por el virus de retículoendoteliosis como una fuente de interferón gama (IFN-&ggr;), disminuye la replicación subsecuente de los esporozoitos en las células. Para identificar la presencia de IFN-&ggr;, se combinaron cuatro actividades típicas de IFN-&ggr;: inhibición del efecto citopático del virus de estomatitis vesicular sobre fibroblastos tratados con IFN-&ggr;, actividad citostática de macrófagos activados por IFN-&ggr;, inducción de la expresión del antígeno del complejo mayor de histocompatibilidad II sobre macrófagos y fibroblastos activados con IFN-&ggr;, e inducción de la producción de nitrito en los macrófagos. Se demostró que los fibroblastos de pollo y los macrófagos poseen una capacidad microbiostática una vez que son capaces de prevenir la replicación intracelular de Eimeria tenella después de la activación con sobrenadantes de cultivos identificados con fuerte actividad de IFN-&ggr;.</P><P><B>[Romanized Abstract]</B></P><P>Pretreatment of chicken macrophages or fibroblasts with supernatants from concanavalin A-stimulated spleen cells or from the virus-transformed cell line reticuloen-dotheliovirus as source of interferon gamma (IFN-g) slows down subsequent sporozoite replication in the cells. To identify the presence of IFN-g, we combined four typical activities of IFN-g: inhibition of cytopathic effect of vesicular stomatitis virus on IFN-g-treated fibroblasts, cytostatic activity of IFN-g-activated macrophages, induction of major histocompatibility complex II antigen expression on IFN-g-activated fibroblasts and macrophages, and induction of nitrite production in macrophages. We have shown that chicken fibroblasts and macrophages possess a microbiostatic capacity once they are able to prevent the otherwise unchecked intracellular replication of Eimeria tenella following activation with culture supernatants identified as containing a strong IFN-g activity. /// El pretratamiento de macrofagos aviares o de fibroblastos con sobrenadantes de celulas esplenicas estimuladas con concalabalina A o de una linea celular transformada por el virus de reticuloendoteliosis como una fuente de interferon gama (IFN-g), disminuye la replicacion subsecuente de los esporozoitos en las celulas. Para identificar la presencia de IFN-g, se combinaron cuatro actividades tipicas de IFN-g: inhibicion del efecto citopatico del virus de estomatitis vesicular sobre fibroblastos tratados con IFN-g, actividad citostatica de macrofagos activados por IFN-g, induccion de la expresion del antigeno del complejo mayor de histocompatibilidad II sobre macrofagos y fibroblastos activados con IFN-g, e induccion de la produccion de nitrito en los mac

      • Boolean Function Decomposition in Symbolic FSM Traversal

        Cabodi, Gianpiero,Camurati, Paolo,Quer, Stefano 대한전자공학회 1993 ICVC : International Conference on VLSI and CAD Vol.3 No.1

        The state space explosion due to memory elements makes symbolic traversal techniques best suited to control dominated circuits, rather than data paths. To take the latter ones into account, auxiliary variables are used to partition a Boolean function in simpler ones and to express it implicitly as a composition. Experimental results demonstrate the benefits both in terms of memory requirements and CPU time.

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