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Longbin Qi,Yunxia Hu,Qingzhi Chai,Qun Wang 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.72 No.-
The anti-biofouling performance of silver nanoparticles (Ag NPs) modified polyethersulfone (PES)membrane was evaluated during the concentration of fermentation broth. The Ag NPs containingmembrane did not exhibit biofouling mitigation performance during thefirstfiltration cycle, but couldhelp to recover waterflux upon physical cleaning. After threefiltration-clean cycles, the Ag NPscontaining membranes presented higher waterflux and slowerflux decline than the control membraneswithout Ag NPs. Ag NPs on the membrane surface facilitated the effective removal of cake layer. Moreover, the Ag NPs-containing membrane had no negative effects on the activities of bacteria infermentation broth.
Yao Zhiming,Fan Jingyan,Dai Jun,Yu Chen,Zeng Han,Li Qingzhi,Hu Wei,Yan Chaoyue,Hao Meilin,Li Haotian,Li Shuo,Liu Jie,Huang Qi,Li Lu,Zhou Rui 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.6
Tylosin is a potent veterinary macrolide antibiotic produced by the fermentation of Streptomyces fradiae; however, it is necessary to modify S. fradiae strains to improve tylosin production. In this study, we established a high-throughput, 24-well plate screening method for identifying S. fradiae strains that produce increased yields of tylosin. Additionally, we constructed mutant libraries of S. fradiae via ultraviolet (UV) irradiation and/or sodium nitrite mutagenesis. A primary screening of the libraries in 24-well plates and UV spectrophotometry identified S. fradiae mutants producing increased yields of tylosin. Mutants with tylosin yield 10% higher than the wild-type strain were inoculated into shake flasks, and the tylosin concentrations produced were determined by highperformance liquid chromatography (HPLC). Joint (UV irradiation and sodium nitrite) mutagenesis resulted in higher yields of mutants with enhanced tylosin production. Finally, 10 mutants showing higher tylosin yield were re-screened in shake flasks. The yield of tylosin A by strains UN-C183 (6767.64 ± 82.43 μg/ml) and UN-C137 (6889.72 ± 70.25 μg/ml) was significantly higher than that of the wild-type strain (6617.99 ± 22.67 μg/ml). These mutant strains will form the basis for further strain breeding in tylosin production.