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Qing-Lei Zeng,Bin Yang,Hong-Qi Sun,Guo-Hua Feng,Lei Jin,Zheng-Sheng Zou,Zheng Zhang,Ji-Yuan Zhang,Fu-Sheng Wang 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.1
Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-α/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatmentnaive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ζ expression on CD8+ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-1+ cells were closely associated with the histological activity index in CHC. The TCR ζ chain was significantly downregulated on hepatic CD8+ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ζ chain expression in CD8+ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ζ chain expression.
Zeng, Qing-Lei,Yang, Bin,Sun, Hong-Qi,Feng, Guo-Hua,Jin, Lei,Zou, Zheng-Sheng,Zhang, Zheng,Zhang, Ji-Yuan,Wang, Fu-Sheng Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1
Myeloid-derived suppressor cells (MDSCs) play an important role in impairing the function of T cells. We characterized MDSCs in two chronic hepatitis C (CHC) cohorts: a cross-sectional group that included 61 treatment-naive patients with CHC, 14 rapid virologic response (RVR) cases and 22 early virologic response (EVR) cases; and a longitudinal group of 13 cases of RVR and 10 cases of EVR after pegylated-interferon-${\alpha}$/ribavirin treatment for genotype 1b HCV infection. Liver samples from 32 CHC patients and six healthy controls were subjected to immunohistochemical analysis. MDSCs frequency in treatment-naive CHC was significantly higher than in RVR, EVR, or healthy subjects and was positively correlated with HCV RNA. Patients infected with HCV genotype 2a had a significantly higher frequency of MDSCs than those infected with genotype 1b. Decreased T cell receptor (TCR) ${\zeta}$ expression on $CD8^+$ T cells was significantly associated with an increased frequency of MDSCs in treatment-naive CHC patients and was restored by L-arginine treatment in vitro. Increased numbers of liver arginase-$1^+$ cells were closely associated with the histological activity index in CHC. The TCR ${\zeta}$ chain was significantly downregulated on hepatic $CD8^+$ T cells in CHC. During antiviral follow up, MDSCs frequency in peripheral blood mononuclear cells was directly correlated with the HCV RNA load in the plasma and inversely correlated with TCR ${\zeta}$ chain expression in $CD8^+$ T cells in both RVR and EVR cases. Notably, the RVR group had a higher frequency of MDSCs at baseline than the EVR group. Collectively, this study provides evidence that MDSCs might be associated with HCV persistence and downregulation of CD8 ${\zeta}$ chain expression.
Two new neolignans from Patrinia scabra with potent cytotoxic activity against HeLa and MNK-45 cells
Lei Di,Guo-Qing Yan,Ling-Yu Wang,Wei Ma,Kai-Jin Wang,Ning Li 대한약학회 2013 Archives of Pharmacal Research Vol.36 No.10
Two new neolignans, patrineolignan A (1) andpatrineolignan B (2), together with seven known lignans,were isolated from the 90 % aqueous EtOH extract of theroots of Patrinia scabra. Their structures were elucidatedon the basis of spectroscopic data (HRESIMS, IR, 1D and2D NMR) and comparison with literature data. The twonew neolignans were evaluated in vitro for cytotoxicproperties against human cervical carcinoma HeLa cell lineand gastric carcinoma MNK-45 cell line using the microculturetetrazolium assay, and both 1 and 2 exhibitedstrongly cytotoxic activity against the two tumor cell lines.
Lei Wang,Shangjun Zhu,Chuang Jiang,Jingyu Li,Kegui Jiang,Chaoqun Teng,Tao Wei,Qing-biao Guo 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.11
When 3D laser scanning technology is used to monitor the surface deformation of mining subsidence in mining area, the surface of the working face is covered with a large number of vegetation, and the surface water accumulates above the working face, which makes the point cloud data obtained by the 3D laser scanning difficult to denoise, or even missing. At this time, the conventional 3D laser scanning technology can not obtain the surface deformation field of mining subsidence. Aiming at the above problems, the 3D laser scanning monitoring method for mining subsidence based on the auxiliary for PIM proposed (3DLS-PIM). Firstly, this paper introduces the PIM prediction model. Secondly, the mining subsidence observation equation based on 3DLS-PIM is constructed, and then the prediction parameters of PIM are solved based on quantum particle swarm optimization (QPSO). Finally, according to the PIM and its parameters, the mining subsidence surface deformation basin is predicted and obtain the surface deformation field of mining subsidence. Robust experiments show that QPSO has a certain ability to resist random errors and gross errors. The results of engineering application show that the mining area 3D deformation monitoring method proposed in this paper has certain engineering application value.
Lei Lyu,Ya Xiong Yao,Er Peng Liu,Yan Ping Zhang,Hui Jie Hu,Feng Ping Ji,Qing Song Pu,Xing Huan Yang,Qing Wei Wang,Yan Wang,Jian Guo Wen 대한배뇨장애요실금학회 2022 International Neurourology Journal Vol.26 No.1
Purpose: To identify more accurate predictors of upper urinary tract dilatation (UUTD) in neurogenic bladder (NB) children, we studied the relationship among urodynamic parameters at different bladder filling stages, detrusor leak point pressure (DLPP) and UUTD. Methods: A total of 158 children (3–16 years) with NB were included and then divided into 2 groups according to whether their NB diagnosis was complicated with UUTD: the UUTD group (39 patients) and those without UUTD group (control group, 119 patients). The bladder filling phase was divided into 3 equal parts: the early, middle, and end filling stages. The bladder compliance (BC) and detrusor pressure (△Pdet) at each phase and DLPP at the end filling stage were recorded. Results: A BC<8 mL/cm H2O both in the middle and end stages is more specific than a BC<9 mL/cm H2O in the end stage (72%, 73%, vs. 66%), and △Pdet >8 cm H2O in the early stage, 20 cm H2O in the middle stage and 25 cm H2O in the end stage are more sensitive than △Pdet >40 cm H2O in the end stage (82%, 85%, 85%, vs. 49%). A DLPP cutoff value of 20 cm H2O showed higher sensitivity for predicting UUTD than 40 cm H2O. Conclusions: Low BC and a high △Pdet in the middle and end filling stages are more accurate factors than classic indicators for predicting UUTD. In addition, a DLPP value of >20 cm H2O in the end bladder filling stage shows high sensitivity.
Guo-Hua Feng,Fu-Sheng Wang,Ji-Yuan Zhang,Qing-Lei Zeng,Lei Jin,Junliang Fu,Bin Yang,Ying Sun,Tianjun Jiang,Xiangsheng Xu,Zheng Zhang,Jinhong Yuan,Liyuan Wu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4
Interleukin-21 (IL-21)+CD4+ T cells are involved in the immune response against hepatitis B virus (HBV) by secreting IL-21. However, the role of IL-21+CD4+ T cells in the immune response against chronic hepatitis C (CHC) virus infection is poorly understood. This study aimed to investigate the role of IL-21+CD4+ T cells in CHC patients and the potential mechanisms. The study subjects in-cluded nineteen CHC patients who were grouped by viral load (low, < 106 RNA copies/ml, n = 8; high, > 106 RNA copies/ml, n = 11). The peripheral frequency of HCV-specific IL-21+CD4+ T cells was higher in the low viral load group and was negatively correlated with the serum HCV RNA viral load in all CHC patients. Meanwhile, IL-21+ cells accumulated in the liver in the low viral load group. In vitro, IL-21 treatment increased the expression of proliferation markers and cytolytic molecules on HCV-specific CD8+ T cells. In summary, these findings suggest that HCV-specific IL-21+CD4+ T cells might contribute to HCV control by rescuing HCV-specific CD8+ T cells in CHC patients.
Wenming Guo,Hanning Xiao,Haibo Lei,Pengzhao Gao,Wen Xie,Qing Li 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.6
In this paper, the influence of SiO2 on the microstructure and consolidation mechanism of recrystallized silicon carbide (RSiC)was studied by comparing the relationship of the weight losses and microstructural evolution with the SiO2 contents at different firing temperatures. The results showed that the presence of SiO2 resulted in a basic weight loss proportional to the SiO2 content and an additional weight loss independent of the SiO2 content. The consolidation mechanism of SiC was not altered by the introduction of SiO2, involving surface diffusion at low temperatures and an evaporation-condensation process at the high temperature, while the residual ambient atmosphere primarily including SiO(g), Si2C(g) and Si(g) inhibited the recrystallization of SiC by altering the mass transport from SiC2(g), Si2C(g) and Si(g) for pure SiC to that combined with the gaseous transport of SiO(g), Si2C(g), Si(g) and SiC(g), and the surface diffusion of C(s) at the high temperature.
Wu, Guo-Qiu,Liu, Nan-Nan,Xue, Xiu-Lei,Cai, Li-Ting,Zhang, Chen,Qu, Qing-Rong,Yan, Xue-Jiao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10
Background: This study was aimed to establish a novel method to simultaneously detect expression of four genes, ribonucleotide reductase subunit M1(RRM1), X-ray repair cross-complementing gene 1 (XRCC1), thymidylate synthase (TS) and class III ${\beta}$-tubulin (TUBB3), and to assess their application in the clinic for prediction of response of non-small cell lung cancer (NSCLC) to chemoradiotherapy. Materials and Methods: We have designed four gene molecular beacon (MB) probes for multiplex quantitative real-time polymerase chain reactions to examine RRM1, XRCC1, TUBB3 and TS mRNA expression in paraffin-embedded specimens from 50 patients with advanced or metastatic carcinomas. Twenty one NSCLC patients receiving cisplatin-based first-line treatment were analyzed. Results: These molecular beacon probes could specially bind to their target genes in homogeneous solutions. Patients with low RRM1 and XRCC1 mRNA levels were found to have apparently higher response rates to chemoradiotherapy compared with those with high levels of RRM1 and XRCC1 expression (p<0.05). The TS gene expression level was not significantly associated with chemotherapy response (p>0.05). Conclusions: A method of simultaneously detecting four molecular markers was successfully established and applied for evaluation of chemoradiotherapy response. It may be a useful tool in personalized cancer therapy.