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Guan Yongchao,Zhang Meng,Wang Yingda,Liu Zhongzhuo,Zhao Zelin,Wang Hong,An Dingjie,Qian Aidong,Kang Yuanhuan,Sun Wuwen,Shan Xiaofeng 한국미생물학회 2022 The journal of microbiology Vol.60 No.12
Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (ΔascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ΔascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ΔascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ΔascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in crucian carp after the infection of the ΔascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.
Mycobacterium vaccae induces a strong Th1 response that subsequently declines in C57BL/6 mice
Lijiao Zhang,Yanlong Jiang,Ziyin Cui,Wentao Yang,Limin Yue,Yingcong Ma,Shaohua Shi,Chun-fang Wang,Chun-feng Wang,Aidong Qian 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.4
Mycobacterium (M.) vaccae is a fast-growing species of saprophytic bacteria that is widely distributed. To understand the host immuneresponses induced by M. vaccae isolated from bovine submaxillary lymph nodes, C57BL/6 mice were infected with reference strain M. bovisBacillus Calmette-Guérin (BCG) and isolated M. vaccae using intraperitoneal injections. Comparison of the bacterial replication and organpathology between M. vaccae and M. bovis BCG revealed that M. vaccae was more malignant than M. bovis in mice. We also demonstratedthat serum from the M. vaccae-infected mice contained a higher expression level of gamma-interferon (IFN-), tumor necrosis factor alpha,monocyte chemoattractant protein-1, interleukin (IL)-4, IL-12, IL-10 and transforming growth factor beta than did the other groups, especiallyafter week 4. Furthermore, when the numbers of CD3+CD4+IFN-+ and CD3+CD4+IL4+ cells in the infected mice were observed by flowcytometry, we found that a powerful T helper 1 (Th1) response was induced by M. vaccae infection, which was associated with the emergenceof CD3+CD4+IFN-+ cells. However, the Th1 response declined over time, which was associated with appearance of the CD4+CD25+FoxP3+and CD4+CD25+CD152+Treg cell reaction. In addition, a strong Th2 response was found. Finally, we found that M. vaccae infection increasedthe production of type I IFNs, which was associated with a reduced Th1 response.