Establishing a submucosal endoscopy program in a gastrointestinal unit

Poornima Varma,Payal Saxena 소화기인터벤션의학회 2020 International journal of gastrointestinal interven Vol.9 No.2

Establishing a submucosal endoscopy program in a new unit is a difficult but exciting task. There are several key steps and teams involved in the success and failure of this endeavour. Careful thought, planning and implementation at the start will allow an easier transition to the development and progression of this program. An interested gastrointestinal unit with skilled proceduralists holds the key to the commencement of a submucosal program. The opportunity for ongoing learning and training through animal models and simulations, are important for proceduralists to continue to enhance their skills and train other staff. A collaborative team with surgical, nursing and technical support, hospital board approval, and credentialing, as well as the acquisition of appropriate equipment are essential components.

Nerve injury induced protein-1 (Ninjurin 1) plays a crucial role in axonal regeneration and cell trafficking post-spinal cord injury in rats

Poornima Ekanayake,Jeongtae Kim,Meejung Ahn,Yuna Choi,Taekyun Shin 한국실험동물학회 2019 한국실험동물학회 학술발표대회 논문집 Vol.2019 No.1

Immunohistochemical localization of nerve injury-induced protein-1 in mouse tissues

Poornima Ekanayake,Meejung Ahn,김정태,Taekyun Shin 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.4

Nerve injury-induced protein (Ninjurin)-1 is a cell adhesion molecule that is upregulated in neurons and Schwann cells after transection injury in rats. In this study, we investigated the localization of Ninjurin-1 in various tissues, including the cerebrum, sciatic nerve, spleen, lung, stomach, ileum, colon, liver, pancreas, kidney, testis, and skin in C57BL/6 mice, using Western blotting and immunohistochemistry. Western blot analysis showed that Ninjurin-1 was differentially expressed among organs. Ninjurin-1 was abundant in skin and ileum, weakly expressed in cerebrum, and moderately expressed in the other organs studied. Immunohistochemical analysis largely confirmed the results of the western blot analysis with often localization of Ninjurin-1 in the regions with abundant connective tissues. In addition, Ninjurin-1 was differentially expressed in various cell types in the tissues under the investigation. These findings suggest that Ninjurin-1 may play organ-specific roles in development and homeostasis of many organs.

Establishing a submucosal endoscopy program in a gastrointestinal unit

Poornima Varma,Payal Saxena 소화기인터벤션의학회 2020 Gastrointestinal Intervention Vol.9 No.2

Establishing a submucosal endoscopy program in a new unit is a difficult but exciting task. There are several key steps and teams involved in the success and failure of this endeavour. Careful thought, planning and implementation at the start will allow an easier transition to the development and progression of this program. An interested gastrointestinal unit with skilled proceduralists holds the key to the commencement of a submucosal program. The opportunity for ongoing learning and training through animal models and simulations, are important for proceduralists to continue to enhance their skills and train other staff. A collaborative team with surgical, nursing and technical support, hospital board approval, and credentialing, as well as the acquisition of appropriate equipment are essential components.

Acute MPTP treatment impairs dendritic spine density in the mouse hippocampus

Poornima D. E. Weerasinghe-Mud,Mary Jasmin Ang,Mai Wada,Sung-Ho Kim,Taekyun Shin,Miyoung Yang,Changjong Moon 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Among the animal models of Parkinson"s disease (PD), the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned mouse model has shown both dopaminergic (DA) damage and related motor control defects, as observed in patients with PD. Recent studies have suggested that the DA system interacts with the synaptic plasticity of the hippocampus in PD. However, little is known about how alterations in the hippocampal structural plasticity are affected by the DA damage in MPTP-lesioned models. In the present study, we investigated alterations in dendritic complexity and spine density in the mouse hippocampus following acute MPTP treatment (22 mg/kg, i.p., four times/day, 2-h intervals). We confirmed that acute MPTP treatment significantly decreased initial motor function and reduced persistently the number of tyrosine hydroxylase-positive DA neurons in the substantia nigra. Golgi staining showed that acute MPTP treatment significantly reduced the spine density of neuronal dendrites in the cornu ammonis 1 (CA1) apical/basal and dentate gyrus (DG) subregions of the mouse hippocampus at 8 and 16 days after treatment, although it did not affect dendritic complexity (e.g., number of crossing dendrites, total dendritic length, and branch points per neuron) in both CA1 and DG subregions at all time points after treatment. Therefore, the present study provides anatomical evidence that acute MPTP treatment affects synaptic structure in the hippocampus during the late phase after acute MPTP treatment in mice, independent of any changes in the dendritic arborization of hippocampal neurons. These findings offer data for the ability of the acute MPTP-lesioned mouse model to replicate the non-nigrostriatal lesions of clinical PD.

Cloud-based Home Energy Management (HEM) and Modeling of Consumer Decisions

Poornima Padmanabhan,Gary R. Waissi 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.8

With the increasing number of smart home devices and the widespread availability of broadband communications, intelligent products built on the computational power and scalability of cloud, have the potential to reach beyond traditional markets. Cloud-based Home Energy Management (HEM) paradigm can provide intuitive and automated services that can not only save money and energy but also improve the quality of lives of consumers. This paper provides a detailed summary of the literature review of HEM and proposes (1) a conceptual smart home model with a special integration of solar powered devices with the cloud, (2) a limited consumer survey, and (3) a Multi-Attribute Modeling (MAM) with an example application for consumer adoption of Home Energy Management System (HEMS) and allied devices. The results of literature research and an online survey point out that the surveyed consumers acknowledge the importance of energy efficiency in their daily lives and lean towards adopting cloud-based smart home devices for their needs. The bottom line for adopting HEMS is the availability of secure, robust and cost-effective solutions coupled with considerable money-saving potential.

Cytomegalovirus Infections in Solid Organ Transplantation: A Review

Poornima Ramanan,Raymund R Razonable 대한감염학회 2013 Infection and Chemotherapy Vol.45 No.3

Cytomegalovirus (CMV) continues to have a tremendous impact in solid organ transplantation despite remarkable advances in its diagnosis, prevention and treatment. It can affect allograft function and increase patient morbidity and mortality through a number of direct and indirect effects. Patients may develop asymptomatic viremia, CMV syndrome or tissue-invasive disease. Late-onset CMV disease continues to be a major problem in high-risk patients after completion of antiviral prophylaxis. Emerging data suggests that immunologic monitoring may be useful in predicting the risk of late onset CMV disease. There is now increasing interest in the development of an effective vaccine for prevention. Novel antiviral drugs with unique mechanisms of action and lesser toxicity are being developed. Viral load quantification is now undergoing standardization, and this will permit the generation of clinically relevant viral thresholds for the management of patients. This article provides a brief overview of the contemporary epidemiology, clinical presentation, diagnosis, prevention and treatment of CMV infection in solid organ transplant recipients.

Spray-freeze-drying as emerging and substantial quality enhancement technique in food industry

Poornima Singh,Vinay Kumar Pandey,Rahul Singh,Aamir Hussain Dar 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.2

Spray freeze drying is an emerging technology in the food industry with numerous applications. Its ability to preserve food quality, maintain nutritional value, and reduce bulk make it an attractive option to food manufacturers. Spray freeze drying can be used to reduce the water content of foods while preserving the shelf life and nutritional value. Spray freeze-drying of food products is a process that involves atomizing food into small droplets and then flash-freezing them. The frozen droplets are then placed in a vacuum chamber and heated, causing the liquid to evaporate and the solid particles to become a dry powder. Spray freeze drying has become a valuable tool for the food industry through its ability to process a wide range of food products. This review’s prime focus is understanding spray freeze-dried approaches and emphasizing their applicability in various products.

Role of STAT3 Phosphorylation in Ethanol-Mediated Proliferation of Breast Cancer Cells

Poornima devi Narayanan,Sangeetha Kadapakkam Nandabalan,Lakshmi Subhadra Baddireddi 한국유방암학회 2016 Journal of breast cancer Vol.19 No.2

Purpose: In this study, we investigated the molecular mechanism involved in ethanol (EtOH)-mediated proliferation of breast cancer cells. Methods: EtOH concentration was optimized by studying its effect on cell proliferation in MCF-7 and MDA MB-231 cells. We used flow cytometry and immunoblot analysis to evaluate the increased proliferation caused by the optimized concentrations of EtOH. The mechanism of EtOH-mediated proliferation was determined using reactive oxygen species (ROS) release assay, reverse transcription polymerase chain reaction, and immunoblot studies. Gene silencing followed by quantitative real-time polymerase chain reaction studies and inhibitor studies indicated the involvement of signal transducer and activator of transcription 3 (STAT3) in EtOH-mediated breast cancer proliferation. Results: Exposure to EtOH caused an increase in cell proliferation and an accumulation of cells in S-phase in MCF-7 (347 μM EtOH) and MDA MB-231 (173 μM EtOH) cells. Additionally, increased release of ROS and the expression of pro-inflammatory cytokines, such as interleukin 6 and tumor necrosis factor α, confirmed that the proliferation was induced by the ROS-linked inflammatory response in breast cancer. The proinflammatory response was followed by phosphorylation of STAT3. The importance of STAT3 activation in EtOH-mediated proliferation was confirmed through the silencing of STAT3, followed by an investigation on the expression of cyclins and matrix metalloproteinases. Finally, studies using specific inhibitors indicated that the EtOH-mediated effect on STAT3 activation could be regulated by phosphoinositide- 3-kinase and Janus kinase 2. Conclusion: The study demonstrates the involvement of STAT3 signaling in EtOH-mediated breast cancer proliferation.

ARPES study of Ultrathin Fe Grown on Cu (001) surface

L. Poornima,Y. R. Oh,Y. S. Park,W. Kim,C.G. Kim,J. Hong,Chanyong Hwang 한국진공학회 2011 한국진공학회 학술발표회초록집 Vol.40 No.0