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Park, Yoo-Hoi,Kim, Jung-Ae,Baek, Jin-Hyen,Jung, Eun-Jin,Kim, Tae-Hyong,Suh, Hongsuk,Park, Myung-Hwan,Kim, Kyu-Won The Pharmaceutical Society of Korea 1997 Archives of Pharmacal Research Vol.20 No.1
The effects of ursodeoxycholic acid (UDCA) and its novel derivative, named as HS-1030, on the proliferation of HepG2, human hepatocellular carcinoma cells were investigated. Whereas UDCA had no significant effect in a concentration range we have tested, HS-1030 inhibited the proliferation of HepG2 cells in a concentration dependent manner. Surprisingly, HS-1030 had no effect on the proliferation of Human Chang liver cell which is a normal liver cell line. We also found that proliferation-inhibitory effect of HS-1030 was due to the induction of apoptosis of HepG2 cells, which was confirmed by observing the internucleosomal DNA fragmentation and morphological changes (ie., cell shrinkage, nuclear condensation and the formation of apoptotic bodies). These results suggest that HS-1030 may be a good candidate as a drug for the treatment of liver cancer.
Park, Yoo-Hoi,Kim, Jung-Ae,Baek, Jin-Hyen,Jung, Eun-Jin,Kim, Tae-Hyong,Suh, Hongsuk,Park, Mying-Hwan,Kim, Kyu-Won 영남대학교 약품개발연구소 1997 영남대학교 약품개발연구소 연구업적집 Vol.7 No.-
The effects of ursodeoxycholic acid (UDCA) and its novel derivative, named as HS-1030, on the proliferation of HepG2, human hepatocellular carcinoma cells were investigated. Whereas UDCA had no significant effect in a concentration range we have tested, HS-1030 inhibited the proliferation of HepG2 cells in a concentration dependent manner. Surprisingly, HS-1030 had no effect on the proliferation of Human Chang liver cell which is a normal liver cell line. We also found that proliferation-inhibitory effect of HS-1030 was due to the induction of apoptosis of HepG2 cells, which was confirmed by observing the internucleosomal DNA fragmentation and morphological changes (i.e, cell shrinkage, nuclear condensation and the formation of apoptotic bodies). These results suggest that HS-1030 may be a good candidate as a drug for the treatment of liver cancer.
朴文香,金泰辰,朴錫濟,玄九源,金聖旭,趙炳鎬,柳熙倬,金亨會,姜熙涉,金榮哲 中央醫學社 1973 中央醫學 Vol.24 No.3
Malignant melanoma is known to metastasize to all organs of the human body and' its manifestations are protean. The gall bladder is said to be involved in 15 per cent of fatal cases of malignant melanoma implanting on serosal surface is majority. However, rarely the metastatic lesions were observed in the mucosa of the gall bladder without showing any ante-mortem history of suggestive acute or subacute cholecystitis. This report is concerned with a case of malignant melanoma metastatic to the mucosa of the gall bladder showing multiple tumor ulceration and gall stones in 40 years old male. Clinical history revealed recurrent colicky pain for 8 months and weight loss of 15 kg. during the period. A green bean sized nevus is noted in the back and no metastatic lesion is observed in the abdominal cavity. The patient was discharged on the 8th day after cholesystectomy.
YOO, Jae-Gyu,HUR, Chang-Gi,PARK, Mi-Rung,PARK, Jong-Yi,HWANG, Kyu-Chan,KIM, Jae-Hwan,KIM, Jin-Hoi,CHO, Seong-Keun Japanese Society of Veterinary Science 2012 The Journal of veterinary medical science Vol.74 No.4
<P>The objective of this study was to evaluate the effect of electrical stimulation (EST) on pronuclear formation, chromosomal constitution, and developmental capability among <I>in vitro</I> matured pig oocytes following intracytoplasmic sperm injection (ICSI). After ICSI, the oocytes were randomly distributed and cultured into 3 groups: the EST activated ICSI group, non-activation ICSI group, and <I>in vitro</I> fertilization (IVF) group. The proportion of oocytes in which 2 pronuclei were formed in ICSI groups was significantly higher in the former groups than in the IVF group (96.2 and 93.5 vs. 64.5%, respectively, <I>P</I><0.05). The cleavage rate was significantly higher in EST activated ICSI group (78.6%) than in the IVF and non-activated ICSI groups (51.8 and 46.0%, respectively, <I>P</I><0.05), as was the proportion of oocytes that developed to the blastocyst stage at day 7 (18.9 vs. 11.6 and 9.1%, respectively, <I>P</I><0.05). Diploid blastocysts were observed in 52.4, 63.0, and 65.2% of oocytes in the IVF, activated, and non-activated ICSI groups, respectively. Eight out of 23 gilts (34.8%) were confirmed to be pregnant in activated ICSI groups, but none of these pregnancies were carried to term. These results show that oocyte activation after ICSI is effective in elevating the cleavage rate and blastocyst development, while ensuring normal chromosome composition. Further research is needed to determine the pregnancy maintenance requirements for ICSI-embryos in pigs.</P>
PDK4 Deficiency Suppresses Hepatic Glucagon Signaling by Decreasing cAMP Levels
Park, Bo-Yoon,Jeon, Jae-Han,Go, Younghoon,Ham, Hye Jin,Kim, Jeong-Eun,Yoo, Eun Kyung,Kwon, Woong Hee,Jeoung, Nam-Ho,Jeon, Yong Hyun,Koo, Seung-Hoi,Kim, Byung-Gyu,He, Ling,Park, Keun-Gyu,Harris, Robert American Diabetes Association 2018 Diabetes Vol.67 No.10
<P>In fasting or diabetes, gluconeogenic genes are transcriptionally activated by glucagon stimulation of the cAMP-protein kinase A (PKA)-CREB signaling pathway. Previous work showed pyruvate dehydrogenase kinase (PDK) inhibition in skeletal muscle increases pyruvate oxidation, which limits the availability of gluconeogenic substrates in the liver. However, this study found upregulation of hepatic PDK4 promoted glucagon-mediated expression of gluconeogenic genes, whereas knockdown or inhibition of hepatic PDK4 caused the opposite effect on gluconeogenic gene expression and decreased hepatic glucose production. Mechanistically, PDK4 deficiency decreased ATP levels, thus increasing phosphorylated AMPK (p-AMPK), which increased p-AMPK-sensitive phosphorylation of cyclic nucleotide phosphodiesterase 4B (p-PDE4B). This reduced cAMP levels and consequently p-CREB. Metabolic flux analysis showed that the reduction in ATP was a consequence of a diminished rate of fatty acid oxidation (FAO). However, overexpression of PDK4 increased FAO and increased ATP levels, which decreased p-AMPK and p-PDE4B and allowed greater accumulation of cAMP and p-CREB. The latter were abrogated by the FAO inhibitor etomoxir, suggesting a critical role for PDK4 in FAO stimulation and the regulation of cAMP levels. This finding strengthens the possibility of PDK4 as a target against diabetes.</P>
Ganoderma lucidum Pharmacopuncture for the Treatment of Acute Gastric Ulcers in Rats
Park, Jae-Heung,Jang, Kyung-Jun,Kim, Cheol-Hong,Lee, Yoo-Hwan,Lee, Soo-Jung,Kim, Bum-Hoi,Yoon, Hyun-Min KOREAN PHARMACOPUNCTURE INSTITUTE 2014 Journal of pharmacopuncture Vol.17 No.3
Objectives: The gastric ulcer is a common disorder of the stomach and duodenum. The basic physiopathology of a gastric ulcer results from an imbalance between some endogenous aggressive and cytoprotective factors. This study examined whether Ganoderma lucidum pharmacopuncture (GLP) would provide protection against acute gastric ulcers in rats. Methods: Sprague-Dawley rats were divided randomly into 4 groups of 8 rats each: normal, control, normal saline (NP) and GLP groups. The experimental acute gastric ulcer was induced by using an EtOH/HCl solution and the normal group received the same amount of normal saline instead of ethanol. The NP and the GLP groups were treated once with injections of saline and GLP, respectively. Two local acupoints were used: CV12 (中脘) which is the alarm point of the Stomach Meridian, and ST36 (足三里), which is the sea point of the Stomach Meridian. The stomachs from the rats in each group were collected and analyzed for gross appearance and histology. Also, immunohistochemistry staining for BAX, Bcl-2 and TGF-${\beta}1$ was performed. Results: Histological observations of the gastric lesions in the control group showed comparatively extensive damage of the gastric mucosa and necrotic lesions had penetrated deeply into the mucosa. The lesions were long, hemorrhagic, and confined to the glandular portions. The lesions were measured microscopically by using the clear depth of penetration into the gastric mucosal surface. The length and the width of the ulcer were measured and the inhibition percentage was calculated. Wound healing of the acute gastric ulcer was promoted by using GLP, and significant alterations of indices in gastric mucosa were observed. Such protection was shown by gross appearance, histology and immunohistochemistry staining for BAX, Bcl-2 and TGF-${\beta}1$. Conclusion: These results suggest that GLP administered at CV12 and ST36 can provide significant protection to the gastric mucosa against an ethanol-induced acute gastric ulcer.