http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Chan Hee Park,HyungWoo Lim,HyunWoo Kim,Mi Yeoun Park,Wook-Kyo Lee,Jong Yul Roh,Kyu Sik Chang,E-Hyun Shin 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
The intracellular endosymbiont bacterium Wolbachia is currently considered the most abundant in arthropods. Many Wolbachia manipulate host reproductive systems, it lead to male-killing, cytoplasmic incompatibility, parthenogenesis and feminization of genetic males with large impact on host ecology and evolution in arthropods. In this study, we investigated the distribution of Wolbachia infection in Ae. albopictus according to geographical distribution. We selected 9 areas and collected Ae. albopictus, these areas were distinguished with mountain chain and waterway. Whole genomic DNA were extracted from collected specimens and PCR analysis were accomplished in each specimen using Wolbachia specific primers such as 16S and WSP genes. As a results, we collected 730 Ae. albopictus at least 30 specimens in each area. Wolbachia infection rate showed different patterns between geographical region. Cheonbook, Cheonnam and Yeongdong showed 100% infection rate followed by Jeju (97%), Chungchung (88%), and Gyungnam (85%) with two Wolbachia marker genes. Our results indicated that Ae. albopictus are commonly infected with Wolbachia, it is possible that Wolbachia may act as endosymbiont in Ae. albopictus regardless of geographical region. Although, low infection rate of Wolbachia, we need continuous survey for the evaluation of Wolbachia strain within Ae. albopictus as a vector of dengue fever.
대퇴 골두 골절과 동반된 외상성 고관절 후방 탈구의 치료
박명식 ( Myung Sik Park ),박종혁 ( Jong Hyuk Park ),김도연 ( Do Yeoun Kim ),조홍만 ( Hong Man Cho ) 대한고관절학회 2010 Hip and Pelvis Vol.22 No.4
Purpose: This study examined the treatment of patients with posterior hip dislocation and femoral head fracture, according to the Pipkin classification, and we analyzed their clinical and radiological results. Materials and Methods: The subjects consisted of 45 patients who were treated for posterior hip dislocation and femoral head fracture. The subjects had an average age of 39, and 38 were men and 7 were women. The average follow-up period was 33 months. The cause of injury was traffic accident in 41 cases and falling for the other four cases. According to the classification, there were 26 Type I cases, 5 Type II cases, 1 Type III case and 13 Type IV cases. The treatment methods were selected according to the patients` condition and the form of fracture. The Ebstein criteria and the Merle d`Aubigne and Postel score were used to determine whether there was a combined injury and to examine the clinical and radiological results. Results: All the cases classified as Type I, II and III showed good or excellent clinical results. A primary osteosynthesis was performed on the Type III cases according to the patient`s age and condition, with particularly good results. An anatomical reduction in the early stages affected the clinical results of the Type IV cases. The observed complications were posttraumatic osteoarthritis (38.4%) and avascular necrosis (15.3%). Conclusion: The prognosis was determined by the injury`s initial severity, the time for reduction and the anatomical reduction and firm fixation of the femur and the fracture fragment of the acetabulum.
Development of molecular markers for identification of tsutsugamushi disease vector species
Eun Hee Shin,Won Il Park,Bong Gu Song,Kyu-Sik Chang,Wook-Gyo Lee,E-hyun Shin,Mi-Yeoun Park,Jong Yul Roh 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.10
Trombiculid mites are known to be the vector of tsutsugamushi disease by transmitting Orientia tsutsugamushi to human. Although the classification of trombiculid mites is necessary for vector surveillance, their classification by morphological observation is only possible at the larval stage and not easy because of similar shapes as well as tiny body sizes. Further the classification need the specimen production process, it takes much time and the accuracy of classification is changed according to the technology of the researcher. The internal transcribed spacers (ITS) regions of 8 trombiculid mite species were analyzed by amplification using tick common ITS primer sets. We designed molecular marker sets for the identification of five Leptotrombidium species, the lengths of marker L. orientale (1078 bp), L. pallidum (820 bp), L. palpale (1202 bp), L. scutellare (447 bp) and for L. zetum (621 bp) and three Neotrombicula species, the lengths of marker N. gardellai (264 bp), N. japonica (460 bp) and N. kwangneungensis (309 bp) based on alignment of ITS sequences. The markers will be helpful for exact classification of trombiculid mites. This study is the first report on molecular marker of ITS regions of trombiculid mites.
Seong Yoon Kim,Won Il Park,Bong Gu Song,Kyu-Sik Chang,Wook-Gyo Lee,E-hyun Shin,Mi-Yeoun Park,Young Ran Ju,Jong Yul Roh 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.10
Trombiculid mites are known to be the vector of scrub typhus by transmitting rickettsial pathogen, Orientia tsutsugamushi, to human. In this study, we tried to establish a monitoring system for trombiculid mites using chigger mite collecting traps instead of the conventional rodent-capture method. For selection of collecting points, 10 environmental points were chosen from three regions (Taean, Jinan and Chungju) and two field collections were performed in spring (March-May) and autumn (October-November) seasons from 2013 to 2014. Among 10 environmental points, waterway (37.9%), grass field (28.0%), rice field and field near mountain side (11.4%) and reservoir/wet field (7.3%) showed high collecting rates and they should be included for the representative collecting points for surveillance using chigger mite collecting trap. In order to test the possibility that the dried chigger mites from collecting trap can be used for detection of O. tsutsugamushi, we pooled 30, 10 and 5 chigger mites separately and performed the nested PCR. The infection of O. tsutsugamushi was successfully detected from 5 chigger mites pooling sample. This study shows that chigger mite collecting trap could be an alternative method for monitoring system of scrub typhus vectors.
박태성,이용성,이승연,이승묵,이성곤,강성현,최호식,유동현,장미윤 한국보건통계학회 2001 한국보건정보통계학회지 Vol.26 No.1
Microarrays are novel biotechnologies which are being used increasingly in medical research. By allowing the monitoring of expression levels for thousands of genes simultaneously, such a microarray technique may lead to a more complete understanding of the molecular variations and may detect differentially expressed genes. However, except for some basic classification and clustering analyses, and testing procedures, not many sophisticated statistical analysis methods have been developed. In this paper, we consider a regression model approach to detect a differentially expressed genes in a single microarray and compare it with other approaches.