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Detection by PCR of Psyllid, Diaphorina citri Kuwayama, Vector of Citrus Huanglongbing Disease
Sul kyoung Park,Gi Don Kim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae), is the most important citrus pest, because it serves as a vector of “Candidatus Liberibacter” species that cause huanglongbing disease. Thus, when exporting Rutaceae (citrus), exporting countries put them into quarantine. However, as the size of an imago is so small that only skilled experts can identify it using morphology-based species identification. A PCR-based assay was developed for monitoring psyllids using a rapid, using the DNA extraction from psyllid bodies and PCR amplification. Thus, this study aims to develop a DNA marker system with high species specificity and discrimination power that helps identifying psyllid. We analyzed the base sequence of mitocondria DNA. Based on this sequence, restriction sites were determined and a species particularity primer was made. Several polymorphic regions of mitochondrial DNA of both species were sequenced and used for developing specific restriction sites and polymerase chain reaction (PCR) primers. Also, species-specific PCR primers were devised to develop diagnostic PCR method for identifying the internal feeders. Base sequence results from PCR, which used psyllid species-specific primers, was analyzed by the Mealign program of the CLC Main Workbench analysis software, and used to develop a phylogenic tree. After analyzing this, Hemiptera proved to be allied species.
Park, Sul-Gi,Kim, Sang-Hun,Kim, Kwang-Youn,Yu, Sun-Nyoung,Choi, Hyeun-Deok,Kim, Young-Wook,Nam, Hyo-Won,Seo, Young-Kyo,Ahn, Soon-Cheol Springer-Verlag 2017 PHARMACOLOGICAL REPORTS Vol.69 No.1
<P>Conclusion: ROS-mediated activation of p38/ERK partially contributes to Toyocamycin-induced apoptosis, and p38/ERK MAPKs regulate the ROS production in PC-3 cells. (C) 2016 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Sp. z o.o. All rights reserved.</P>
박민기 ( Min Gi Park ),이지현 ( Ji Hyun Lee ),김성준 ( Sung Jun Kim ),박수호 ( Su Ho Park ),박석기 ( Suk Ki Park ),최준설 ( Joon Sul Choi ),황지연 ( Ji Yeon Hwang ) 영남대학교 의과대학 2017 Yeungnam University Journal of Medicine Vol.34 No.1
Gitelman syndrome is a condition caused by a mutation of the thiazide sensitive Na-Cl cotransporter gene on the distal convoluted tubule. It results in a variety of clinical features, including hypokalemia, hypomagne-semia, hypocalciuria, and metabolic alkalosis. It is often diagnosed in asymptomatic adults presented with unexplained hypokalemia; however, it is sometimes associated with muscular cramps, numbness, fatigue, weakness, or paralysis. We experienced a case of rheumatoid arthritis accompanied by Gitelman syndrome, presented with hand tremor. We diagnosed her using renal clearance study and genetic analysis. Here, we report our experiences regarding this case along with a literature review.
Hak-Jae Chung,NaRae Son,Joo-Hee Han,Chun-Gyu Park,Kyung-Woon Kim,Mi-Ryung Park,In-Sul Hwang,Jin-Ki Park,Gi-Sun Im 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.3
Understanding the behavior of transgenes introduced into oocyte or embryos is essential for evaluating the methodologies for transgenic animal production. To date, many studies have reported the production of transgenic pig embryos with, however, low efficiency in environment of blastocyst production. The aim of present study was to determine the expression and duration of transgene transferred by intracytoplasmic sperm injection-mediated gene transfer (ICSI-MGT). Embryos obtained from the ICSI-MGT procedure were analysed for the expression of GFP and then for the transmission of the transgene. Briefly, fresh spermatozoa were bound to exogenous DNA after treatment by Triton X-100 and Lipofectin. When ICSI-MGT was performed using sperm heads with tails removed, the yield of blastocyst (25.3%), treated with Lipofectin (18.8%) and Triton X-100 (19.2%) were observed. Treatments of Lipofectin or Triton X-100 did not further improve the rates of blastocysts. Moreover, the apoptosis rates of embryos were obtained from the control and LIpofectin groups (8.7%, 9.7%, respectively), but were significantly higher in the Triton X-100 group (13.0%). Our results demonstrated that ICSI-MGT caused minimal damage to oocytes that could develop to full term. Moreover, the embryos derived by ICSI-MGT have shown prolonged exogenous DNA expression during preimplantation stage in vivo. However, more efforts will be required to improve the procedures of both sperm treatments cause of high frequency of mosaicisms.
DEVELOPMENT OF SEMI-VLBI SYSTEM AND OBSERVATION OF SUN AT 21 cm
Park Yong-Sun,Kang Hyun-Woo,Kim Hyo-Uk,Kang Heui-Jeong,Jee Myeong-Keun,Lee Sul-Gi,Ahn Jung-Hyun,Kim Jin-Su,Shin Young-Soo,Kang Song-Hun The Korean Astronomical Society 2006 Journal of The Korean Astronomical Society Vol.39 No.2
We report the development of a semi-VLBI observation system operating at 21 cm and present the measurement of visibility function toward the sun using this system. The system consists of two 2.3 meter antennas with a maximum separation of 35 meter, a conventional high speed data acquisition system, and a set of programs for software correlation. Since two local oscillators of receiver modules are independent, data had to be fringe-fitted to yield the visibility amplitude. It is found that the visibility amplitude decreases and then bounces back as baseline increases. We confirm that solar disk with brighter limb best explains the measured visibility amplitude.
Effect of Caffeine and Theophylline on Sperm Motility during In Vitro Fertilization
In-Sul Hwang,Sung Woo Kim,Dae-Jin Kwon,Tae-Uk Kwak,Seong-Eun Moon,Yu-Ra Kim,Hyun Yang,Mi-Ryung Park,Gi-Sun Im,,Seongsoo Hwang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
The present study was conducted to investigate the effect of caffeine and theophilline on sperm motility during in vitro fertilization (IVF). Briefly, commercial boar semen was centrifuged and resuspended (5x107 sperms/ml) with fertilization medium (mTBM) supplemented with 2 mM caffeine (Caf), 5 mM theophylline (The), 2 mM caffeine + 5mM theophylline (Caf + The), and not supplemented control. The semen parameters of the four groups were analyzed by computer-assisted semen analysis (CASA, Medical Supply Co. Ltd) system at 6 h as time for IVF at 38.5 C under 5 % CO2 in air. The groups were examined 11 semen parameters such as total motility (TM), curvilinier velocity (VCL), straight-line velocity (VSL), average-path velocity (VAP), and hyperactivated (HYP), etc. A total motility of control group (41.3 %) at 6 h showed significantly (P<0.05) higher than those of other groups (Caf, 37.2; The, 35.2; Caf + The, 30.1 %). Results from many other sperm parameters indicated that the theophylline and caffeine negatively affected on sperm motility during IVF. These results suggested that the supplementation of caffeine and/or theophylline was not essential for IVF in pigs. To prove this suggestion, further studies are needed to analyze fertility and embryonic development after IVF with or without the supplementation of caffeine and/or theophylline.
Effect of a short-term in vitro exposure time on the production of in vitro produced piglets
In-Sul Hwang,Dae-Jin Kwon,Tae-Uk Kwak,Joo-Young Lee,Nam-Woong Hyung,Hyeon Yang,Keon Bong Oh,Sun-A Ock,Eung-Woo Park,Gi-Sun Im,Seongsoo Hwang 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.2
Although piglets have been delivered by embryo transfer (ET) with in vitro produced (IVP) embryos and blastocysts, a success rate has still remained lower level. Unlike mouse, human, and bovine, it is difficult to a production of piglets by in vitro fertilization (IVF) because of an inappropriate in vitro culture (IVC) system in pig. Therefore, the present study was conducted to investigate whether minimized exposure time in IVC can improve the pregnancy and delivery rates of piglets. Immediately after IVM, the oocytes were denuded and co-incubated with freshly ejaculated boar semen for 3.5 to 4 hours at 38.5 ˚C under 5% CO2 in air. To avoid long-term exposure to in vitro state, we emitted IVC step after IVF. After that the presumptive zygotes were transferred into both oviducts of the surrogate on the same day or 1 day after the onset of estrus. Pregnancy was diagnosed on day 28 after ET and then was checked regularly every month by ultrasound examination. The 3 out of 4 surrogates were determined as pregnant (75%) and a total of 5 piglets (2 females and 3 males) were delivered at 118.3 ± 2.5 days of pregnancy period. In conclusion, a short-term exposure time may be an important factor in the production of IVP-derived piglets. It can be apply to the in vitro production system of transgenic pig by IVF, cloning, and pronuclear microinjection methods.