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Transgenic Efficiency of FoxN1-targeted Pig Parthenogenetic Embryos
Jae-Hoon Yeo,In-Sul Hwang,Jae Kyung Park,Dae-Jin Kwon,Seoki Im,Eung-Woo Park,Jeong-Woong Lee,Choon-Keun Park,Seongsoo Hwang 한국수정란이식학회 2014 한국동물생명공학회지 Vol.29 No.4
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p<0.05) decreased (4 ng/μl, 51.24%; 8 ng/μl, 40.88%; and 16 ng/μl; 45.22%) compared to no injection group (70.44%). The blastocyst formation rates were also decreased in vector injected 3 groups (4 ng/μl, 7.96%; 8 ng/μl, 6.4%; and 16 ng/μl; 9.04%) compared to no injection group (29.07%). In addition, the blastocyst formation rates between sham injected group (13.51%) and no injection group (29.07%) also showed significant difference (p<0.05). The mutation rates were comparable between groups (4 ng/μl, 18.4%; 8 ng/μl, 12.5%; and 16 ng/μl; 20.0%). The sequencing analysis showed that blastocysts derived from each group were successfully mutated in FoxN1 loci regardless of the vector concentrations. However, the deletion patterns were higher than the patterns of point mutation and insertion regardless of the vector concentrations. In conclusion, we described that cytoplasmic microinjection of FoxN1-targeted CRISPR/Cas9 vector could efficiently generate transgenic pig parthenogenetic embryos in one-step.
( Gwang Woong Go ),( Sangnam Oh ),( Miri Park ),( Gyoungok Gang ),( Danielle Mclean ),( Han Sul Yang ),( Min Ho Song ),( Young Hoon Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.11
In mice, supplementation of t10,c12 conjugated linoleic acid (CLA) increases liver mass and hepatic steatosis via increasing uptake of fatty acids released from adipose tissues. However, the effects of t10,c12 CLA on hepatic lipid synthesis and the associated mechanisms are largely unknown. Thus, we tested the hypothesis that gut microbiota-producing t10,c12 CLA would induce de novo lipogenesis and triglyceride (TG) synthesis in HepG2 cells, promoting lipid accumulation. It was found that treatment with t10,c12 CLA (100 μM) for 72 h increased neutral lipid accumulation via enhanced incorporation of acetate, palmitate, oleate, and 2- deoxyglucose into TG. Furthermore, treatment with t10,c12 CLA led to increased mRNA expression and protein levels of lipogenic genes including SREBP1, ACC1, FASN, ELOVL6, GPAT1, and DGAT1, presenting potential mechanisms by which CLA may increase lipid deposition. Most strikingly, t10,c12 CLA treatment for 3 h increased phosphorylation of mTOR, S6K, and S6. Taken together, gut microbiota-producing t10,c12 CLA activates hepatic de novo lipogenesis and TG synthesis through activation of the mTOR/SREBP1 pathway, with consequent lipid accumulation in HepG2 cells.
조설,박래웅 대불대학교 2003 論文集 Vol.9 No.1
This paper presents a nonlinear adaptive control law methodology for a high angle of attack aircraft, in particular, a modified F-18 aircraft. The nonlinear adaptive controller presented in this paper is based on bilinear prediction model, and is constructed to minimize the difference of Lyapunov function with respect to the control inputs at each step. The physical constraints of inputs such as the limit of range and rate of change are considered importantly. In the paper, the reference trajectory is proposed as an intermediate step that allows the system to follows the command trajectory while meeting a variety of design requirements. This paper shows that nonlinear adaptive control can be utilized effectively to control high performance aircraft such as the F-18 aircraft for rapid maneuvers with large changes in angle of attack.
Effect of a short-term in vitro exposure time on the production of in vitro produced piglets
In-Sul Hwang,Dae-Jin Kwon,Tae-Uk Kwak,Joo-Young Lee,Nam-Woong Hyung,Hyeon Yang,Keon Bong Oh,Sun-A Ock,Eung-Woo Park,Gi-Sun Im,Seongsoo Hwang 한국수정란이식학회 2016 한국동물생명공학회지 Vol.31 No.2
Although piglets have been delivered by embryo transfer (ET) with in vitro produced (IVP) embryos and blastocysts, a success rate has still remained lower level. Unlike mouse, human, and bovine, it is difficult to a production of piglets by in vitro fertilization (IVF) because of an inappropriate in vitro culture (IVC) system in pig. Therefore, the present study was conducted to investigate whether minimized exposure time in IVC can improve the pregnancy and delivery rates of piglets. Immediately after IVM, the oocytes were denuded and co-incubated with freshly ejaculated boar semen for 3.5 to 4 hours at 38.5 ˚C under 5% CO2 in air. To avoid long-term exposure to in vitro state, we emitted IVC step after IVF. After that the presumptive zygotes were transferred into both oviducts of the surrogate on the same day or 1 day after the onset of estrus. Pregnancy was diagnosed on day 28 after ET and then was checked regularly every month by ultrasound examination. The 3 out of 4 surrogates were determined as pregnant (75%) and a total of 5 piglets (2 females and 3 males) were delivered at 118.3 ± 2.5 days of pregnancy period. In conclusion, a short-term exposure time may be an important factor in the production of IVP-derived piglets. It can be apply to the in vitro production system of transgenic pig by IVF, cloning, and pronuclear microinjection methods.
Pyo-Woong Son,Tae Hyun Fang,Sul Gee Park,Younghoon Han,Kiyeol Seo 사단법인 항법시스템학회 2022 Journal of Positioning, Navigation, and Timing Vol.11 No.1
The eLoran system is a high-power terrestrial navigation system that is recognized as the most appropriate alternative to complement the GNSS’s vulnerability to radio frequency interference. Accordingly, Korea has conducted eLoran technology development projects since 2016. The eLoran system developed in Korea provides 20 m positioning accuracy to maritime user in Incheon and Pyeongtaek harbor. To accurately calculate the position with the eLoran signal, it is necessary to apply a compensation method that mitigates the propagation delay. In this paper, we develop the compensation method to mitigate the eLoran signal propagation delay and evaluate the positioning performance in Incheon harbor. The propagation delay due to the terrain characteristics is pre-surveyed and stored in the user receiver. Real-time fluctuations in propagation delay compared to the pre-stored data are mitigated by the temporal correction generated at a nearby differential Loran station. Finally, two performance evaluation tests were performed to verify the positioning accuracy of the Korean eLoran system. The first test took place in December 2020 and the second in April 2021. As a result, the Korean eLoran service has been confirmed to provide 20 m location accuracy without GPS.
FUZZY CONTROL LAW FOR HIGH ANGLE ALTHA AIRCRAFT
조설,박래웅 대불대학교 1999 論文集 Vol.5 No.1
이 논문에서는 고성능, 고기동을 요구하는 항공기에 적용하기 위한 퍼지 제어기설계 방법을 제시한다. 사용된 항공기는 F/A-18 항공기의 수정된 모델이다. 입력으로 사용하는 트러스트 벡터의 크기와 각 변위의 제한, 그리고 승강키이 비선형성을 포함하는 퍼지 제어기를 비례, 적분, 미분기에 근거하여 설계한다. 다충 퍼지 규칙에 근거한 단순한 이득 스케줄 방법을 이용하여 승강키와 트러스트 벡터의 작동 영역을 확장하였다. 퍼지 규칙의 계산 능력을 향상시키기 위해 가속화된 커널 방법을 사용하였다. 시뮬레이션 결과에 의하면 퍼지 제어기도 고기동, 고성능을 요구하는 항공기에 적용할 수 있는 제어기로 개선되고 있다.
Effect of a short-term in vitro exposure time on the production of in vitro produced piglets
Hwang, In-Sul,Kwon, Dae-Jin,Kwak, Tae-Uk,Lee, Joo-Young,Hyung, Nam-Woong,Yang, Hyeon,Oh, Keon Bong,Ock, Sun-A,Park, Eung-Woo,Im, Gi-Sun,Hwang, Seongsoo The Korean Society of Embryo Transfer 2016 한국동물생명공학회지 Vol.31 No.2
Although piglets have been delivered by embryo transfer (ET) with in vitro produced (IVP) embryos and blastocysts, a success rate has still remained lower level. Unlike mouse, human, and bovine, it is difficult to a production of piglets by in vitro fertilization (IVF) because of an inappropriate in vitro culture (IVC) system in pig. Therefore, the present study was conducted to investigate whether minimized exposure time in IVC can improve the pregnancy and delivery rates of piglets. Immediately after IVM, the oocytes were denuded and co-incubated with freshly ejaculated boar semen for 3.5 to 4 hours at $38.5^{\circ}C$ under 5% $CO_2$ in air. To avoid long-term exposure to in vitro state, we emitted IVC step after IVF. After that the presumptive zygotes were transferred into both oviducts of the surrogate on the same day or 1 day after the onset of estrus. Pregnancy was diagnosed on day 28 after ET and then was checked regularly every month by ultrasound examination. The 3 out of 4 surrogates were determined as pregnant (75%) and a total of 5 piglets (2 females and 3 males) were delivered at $118.3{\pm}2.5$ days of pregnancy period. In conclusion, a short-term exposure time may be an important factor in the production of IVP-derived piglets. It can be apply to the in vitro production system of transgenic pig by IVF, cloning, and pronuclear microinjection methods.