저칼슘식이 생쥐 하악골에서 파골세포의 Tartrate저항성 산성인산분해효소 활성에 대한 세포화학적 연구

박은주,임도선,김현만,고재승 대한구강해부학회 1994 대한구강해부학회지 Vol.18 No.1

The present study was carried out to exanine the morphological changes and TRACP (Tartrate resistant acid phosphatase) activity in the resorption of mandibular bones of mouses resulted, established by low calcium diet. Mice were divided into two groups; one was the calcein group for fluorescent microscopy, the other was the non-calcein group for light and electron microscopy. After calcein injection, mice(ICR) were fed either a low calcium diet (0.01% Ca, 0.75% P) or control diet (0.1% Ca. 0.75% P). Non-calcein group were also fed a low calcium diet or control diet. Mice were sacrificed on 3, 7 and 14 days. For fluorescent microscopy study, fixed undecalcified alveolar bones were embedded in Epon 812, and ground sectioned to study the new bone formation after calcein injection. For the TRACP histochemical study, fixed decalcified alveolar bones were incubated in p-NPP (p-nitrophenyl phosphate) media. For the transmission electron microscopic study, incubated alveolar bone were post fixed in OsO_(4), embedded with Epon 812 for the study of TRACP localzation and activity. The observed results were as follows. 1. Low calcium diet induced bone resorption in buccal and lingual sides of alveolar bone, while the lingual side of alveolar bone was not resorbed in the control group. Bone resorption increased over the period of low calcium diet. 2. The alveolar bone proper of the lingual alveolar bone continued to deposit new bone of the periodontal side even after the low calcium diet. 3. After low calcium diet TRACP activity increased in the cytoplasm of osteoclast, extracellular ruffled border. TRACP activity was also found at the eroded bone matrix after bone resorption, and in the cytoplasm of detached osteoclast. In the immature osteoclast enzyme activity was confined in rER. No enzyme activity was found in the osteoblast.

벼 담수골표면산파 재배의 파종방법 및 효과

황동용,박성태,김재현,양세준,김성만 한국국제농업개발학회 2002 韓國國際農業開發學會誌 Vol.14 No.4

담수골 표면산파시 안정입모 확보를 위한 파종 골거리와 깊이 및 파종전 후 물 관리 등에 따른 효과 및 주요 결과를 요약하면 다음과 같다. <적정 골거리 및 골깊이 구명> 1. ㎡당 입모수는 128-168개로 골 깊이 및 골거리에 관계없이 충분한 입모수가 확보되었고, 골내 입모율은 골거리 10㎝, 골깊이 8㎝에서 가장 높았고, 골 형성 정도는 골 거리 15㎝, 골 깊이 8㎝에서 비교적 양호하였다. 2. ㎡당수수는 각각 384∼428개로 처리간에는 뚜렷한 경향이 없었고, 수당립수는 76∼88개로 골거리가 깊을수록 적었고, 등숙율은 77.3∼84.6%로 골 거리간에는 차이가 없었으나 골 깊이간에는 깊을수록 높았다. 3. 담수골표면산파시 쌀 수량은 451∼534㎏/10a로 담수표면산파 448/㎏ 대비 1∼19% 증수되었고, 골 거리 15㎝, 골 깊이 8㎝에서 쌀 수량이 가장 높았다. 4. 담수골표면산파는 담수표면산파에 비해 줄기 매몰심이 깊고 포장 도복피해 정도는 골 깊이가 깊을수록 적었고 담수표면산파는 완전도복 되었다. <골형성후 파종상태 구명> 1. 골 형성정도는 양호하였으며, 종자몰림 및 부묘 정도는 거의 없었고, 골 내입모율은 68∼74%로 산파를 하였으나 어느 정도 조파형태로 입모 되었으며, ㎡당 입모수는 123개였음. 2. 줄기매몰심은 1.6∼2.2㎝로 깊게 매몰되었으며, 포장도복은 거의 없었으나 담수표면산파에서는 심한 도복현상을 보였다. 3. ㎡당수수 및 수당립수는 담수표면산파와 비슷하였으나 등숙율은 82%로 담수표면산파 77%보다 높았으며 쌀 수량은 515㎏/10a으로 담수표면산파 460/㎏ 대비 12% 증수되었다. 4. 농가 현지적응 3개지역 평균 ㎡당 입모수는 144개, 골내 입모율은 75.2%, 줄기 매몰심은 2.0㎝였고, ㎡당 수수는 373개로 기계이앙보다 11개가 많았고, 완전 미율 및 10a 당 백미 수량은 각각 95.8%, 578㎏으로 기계이앙과 비슷하였으며, 식미치는 77%로 기계이앙과 같았다. 5. 따라서 담수골표면산파시의 적정골거리 15㎝, 골깊이 8㎝로 제작된 작조기를 무논정지기에 부착하여 골형성후 파종작업을 했을 때 기존 담수표면산파보다 입모확보가 용이하고 새피해 및 도복피해 경감과 함께 안정 수량확보가 유리한 기술로 생각됨. This experiments was carried out to investigated cultivation effects of water wet furrow broadcasting seeding, a simple and cheap seeding furrow maker, which facilitates rice seeding and addresses lodging problem in water seeding rice was developed. The furrow maker with 432㎝ in length and 20㎝ in width was attached right after the rotary for furrow making. Before seed sowing , puddled soil is hardened for 4∼6 days and seeding furrow is prepared with the furrow maker and than the power duster is used to broadcast the pregerminated seeds. The water wet furrow broadcasting seeding was found to be stable in seedling stand by uniform seed sowing as well as in the reduction of seedling floating and high percentage of inner furrow seedling stand with 68∼74% by water wave movements : resulting to enhanced seedling furrow formation, wind pass, and light penetration. This method also ameliorates lodging problem by burying rice culm 1.6∼2.2㎝ deep as compared with conventional water seeding. Because of such factors, the milled rice yield was increased 1∼19% compared with conventional water seeding with 515㎏/10a.

IEEE 802.11 기반의 멀티 채널 협업 MAC 프로토콜 설계 및 성능 평가

박만규(Man-Kyu Park),박세철(Se-Chul Park),김병철(Byung-Chul Kim),이재용(Jea-Yong Lee),김대영(Dae-Young Kim) 한국통신학회 2008 한국통신학회 학술대회논문집 Vol.2008 No.11

GloMoSim을 이용한 B-MAC 시뮬레이터 구현 및 성능 평가

박만규(Man-Kyu Park),소상호(Sang-Ho So),최윤철(Yun-Chul Choi),김병철(Byung-Chul Kim),이재용(Jea-Yong Lee),김세한(Se-Han Kim),박태준(Tae-Jun park),김내수(Nae-Soo Kim) 한국통신학회 2007 한국통신학회 학술대회논문집 Vol.2007 No.11

저칼슘식이로 사육한 생쥐의 골수세포 배양에 의한 파골세포양세포의 형성

박주철,강선주,임도선,김현만,고재승 대한구강해부학회 1995 대한구강해부학회지 Vol.19 No.1

Although it is now well established that osteoclasts are derived from mononuclear hematopoietic stem cells, most likely within the granulocyte-macrophage lineage, the exact differentiation process of osteoclast precusors has only been partially discovered. This study was carried out to investigate the following topics by examining the osteoclast-like cell formation from the calcium-deficient mouse bone marrow cells; 1) the effect of calcium deficiency on the formation of the mononuclear phagocytes and the mononuclear precusors of the osteoclast in the mouse bone marrow; 2) the effect of TNF (tumor necrosis factor)-α on the differentiation of osteoclast-like cells from the calcium-deficient mouse bone marrow culture; 3) the formation of the osteoclast-like cell from the calcium-deficient mouse bone marrow mononuclear cells and the relationship between the osteoblast cell line(MC3T3E1) and osteoclast-like cell formation; 4) formation of the osteoclast-like cells from the mononuclear cells which were col1ected from PTH-pretreated mouse bone marrow cells to investigate the relationship between PTH and calcium deficiency in osteoclast-like cell formation. Bone marrow cells were isolated from marrow of mouse feeding normal diet or calcium deficient diet for 7 and 14 days. Bone marrow mononuclear cells were fractionated by cetrifugation on Hypaque-Ficoli density gradients. PTH-pretreated mouse bone marrow mononuclear cells were obtained by collecting the nonadherent cells after the culture of normal mouse marrow cells with PTH for 6 days. The prepared cells were cultured on dentin disc at the concentration of 1.5-2 X 10^(6) cells per 0.5ml of a-MEM containing 10% FCS for 3, 5 and 7 days. Thereafter, TRAP staining, NSE staining, effect of calcitonin on the osteoclast-like cell and scanning electron microscope analysis of resorption lacunae on the dentin disc were performed. The results were as follows; 1. Number of the osteoclast precusors and mononuclear phagocytes in bone marrow was increased by calcium-deficient diet. 2. More osteoclast-like cells were formed from the calcium-deficient mouse bone marrow cells than from normal cells. 3. Formation of osteoclast-like cells was significantly stimulated by TNF-α in normal mouse bone marrow cells and calcium-deficient mouse bone marrow cells at concentration of l0ng/ml and 100ng/ml. 4. Osteoblast cell line(MC3T3E1) did not play an important role in osteoclast-like cell formation. 5. Formation of the osteoclast-like cells from the mononuclear cells which were collected from PTH-pretreated mouse bone marrow cells was similar to that of calcium-deficient mouse bone marrow mononuclear cells, which suggests that the effect of calcium deficiency in osteoclast formation may be mediated by PTH.

The Effect of Dietary Fermented Soybean on the Growth Performance and Meat Quality of Pigs

Park, Jung-Min,Shin, Jin-Ho,Bak, Da-Jeong,Lee, Dan-Won,Jeon, Woo-Min,Song, Jea-Chul,SunWoo, Sun-Young,Lyoo, Young-Soo,Kim, Jin-Man Korean Society for Food Science of Animal Resource 2009 한국축산식품학회지 Vol.29 No.3

This study investigated the effects of fermented soybean (FS) on growth and meat quality in pigs. A total thirty-six pigs were divided into 2 groups (2 treatment$\times$18 pigs each) and fed the experimental diets for 4 wk as follows: control (FS free); and T1 (FS 1%). The pigs in T1 had a higher feed efficiency compared with pigs fed control diets. pH was also significantly higher in the T1 group (p<0.05). Water-holding capacity measured in T1 was slightly higher than that of the control. Cooking loss in T1 was significantly lower than controls (p<0.05). CIE $L^*$ and CIE $b^*$ value were significantly higher in the control (p<0.05), but CIE $a^*$ value of T1 was higher than control (p<0.05). The drip loss of T1 were significantly lower than controls (p<0.05). Sensory characteristics of the treatment group showed higher than controls (p<0.05). These results showed that FS could be served as a favorable feed additive and feedstuff for enhancing pork quality.

Infrared Thermography Characterization of Defects in Seamless Pipes Using an Infrared Reflector

Park, Hee-Sang,Choi, Man-Yong,Park, Jeong-Hak,Lee, Jea-Jung,Kim, Won-Tae,Lee, Bo-Young The Korean Society for Nondestructive Testing 2012 한국비파괴검사학회지 Vol.32 No.3

Infrared thermography uses infrared energy radiated from any objects above absolute zero temperature, and the range of its application has been constantly broadened. As one of the active test techniques detecting radiant energy generated when energy is applied to an object, ultrasound infrared thermography is a method of detecting defects through hot spots occurring at a defect area when 15~100 kHz of ultrasound is excited to an object. This technique is effective in detecting a wide range affected by ultrasound and vibration in real time. Especially, it is really effective when a defect area is minute. Therefore, this study conducted thermography through lock-in signal processing when an actual defect exists inside the austenite STS304 seamless pipe, which simulates thermal fatigue cracks in a nuclear power plant pipe. With ultrasound excited, this study could detect defects on the rear of a pipe by using an aluminium reflector. Besides, by regulating the angle of the aluminium reflector, this study could detect both front and rear defects as a single infrared thermography image.

Changes in Oxygen Saturation and Walk in Relation to Smoking and Types of Shoes

( Jea-cheol Park ),( Jong-man Han ),( Woon-soo Cho ),( Yong-nam Kim ) 대한물리치료학회 2015 대한물리치료학회지 Vol.27 No.1

Purpose: The purpose of this study is to examine the effects of types of shoes and distance travelled on oxygen saturation and walk in relation to smoking. Methods: To achieve this goal, the researcher divided 20 normal male adults into two groups: a smoking group (10) and a non-smoking group (10) and used a treadmill to have them walk in intervals of 3 days, a total of 4 times. Results: By analyzing their walk, the following results were obtained: oxygen saturation showed statistically significant results in both the smoking group and the non-smoking group. The smoking group showed significant results for left pace and significant results were achieved for setting foot and shaking in both the smoking group and the non-smoking group. In comparison of oxygen saturation between groups, there were significant results for 3km sneakers walk and 3 km slippers walk. There were significant results for width in 3 km sneakers walk, left and right pace in 3 km slippers walk, and setting foot in 1 km sneakers walk. Conclusion: In conclusion, in the smoking group changes in walk occur if the shoes are more unstable and the distance travelled becomes longer.

Changes in Oxygen Saturation and Walk in Relation to Smoking and Types of Shoes

Park, Jea-Cheol,Han, Jong-Man,Cho, Woon-Soo,Kim, Yong-Nam 대한물리치료학회 2015 대한물리치료학회지 Vol.27 No.1

Purpose: The purpose of this study is to examine the effects of types of shoes and distance travelled on oxygen saturation and walk in relation to smoking. Methods: To achieve this goal, the researcher divided 20 normal male adults into two groups: a smoking group (10) and a non-smoking group (10) and used a treadmill to have them walk in intervals of 3 days, a total of 4 times. Results: By analyzing their walk, the following results were obtained: oxygen saturation showed statistically significant results in both the smoking group and the non-smoking group. The smoking group showed significant results for left pace and significant results were achieved for setting foot and shaking in both the smoking group and the non-smoking group. In comparison of oxygen saturation between groups, there were significant results for 3km sneakers walk and 3 km slippers walk. There were significant results for width in 3 km sneakers walk, left and right pace in 3 km slippers walk, and setting foot in 1 km sneakers walk. Conclusion: In conclusion, in the smoking group changes in walk occur if the shoes are more unstable and the distance travelled becomes longer.

Effect of TGF-β1 on Osteoclast Differentiation

Park, Su-Jin,Ko, Jea-Seung,Kim, Hyun-Man Korean Academy of Oral Biology and the UCLA Dental 2005 International Journal of Oral Biology Vol.30 No.4

Although it has been known that TGF-β1 acts as a crucial cofactor in osteoclast differentiation, its mode of action is still unclear. In the present study, we studied the effect of TGF-β1 on the differentiation of osteoclast depending on the developmental stages. Murine bone marrow cells were induced to differentiate into mature osteoclasts in the presence of receptor activator of NF-κB ligand (RANKL) and macrophage colony stimulating factor (M-CSF). In the early stage of the differentiation TRAP(-) mononuclear precursor cells were obtained from nonadherent M-CSF dependent bone marrow cells, which further differentiated into mature osteoclasts. TGF-β1 stimulated osteoclast differentiation, which was stronger when cells were stimulated by TGF-β1 in the early stage than the later differentiation. TGF-β1 increased the expression of RANK and synergistically stimulated RANKL-induced activation of NF-κB MAP kinase in TRAP(-) mononuclear precursor cells. These results suggest that activation of osteoclast differentiation by TGF-β1 may be ascribed to the both increased expression and activation of RANK in the osteoclast differentiation, especially in the early stage of differentiation.