窒素施肥量 및 菌株가 稻葉部位에 따른 稻熱病의 稻原力과 病斑型에 미치는 影響

白壽鳳 건국대학교 1980 學術誌 Vol.24 No.2

The effect of nitrogen and Pyricularia oryzae strains on disease index and lesion type of leaf blade at different positions and leaf parts in paddy field was examined and the results were as follows 1)The disease index of the upper 1st leaf to 3rd leaf at 2 times of nitrogen was not different from that of the standard nitrogen. The disease index of the upper 4th leaf at 2 times of nitrogen was higher than that of the standard nitrogen and lesion type was brown spot type, chronicity type and acute type. 2)The disease index of the upper part at the standard nitrogen was higher than that of the lower part. The disease index of the middle part at the 2 times nitrogen was highest and that of the lower part was lowest. In the standard nitrogen the upper part showed chronicity type and acute type and the lower part showed few spots of both type. In 2 times nitrogen chronicity type was appeared at the upper and the lower part but few at the middle part, and acute type was appeared at the middle part, but few at the lower part. 3)The disease index of Pyricularia oryzae strains was in order of the first, second, third and fourth leaf from highest to lowest. This tendency was shown only in acute type not in brown spot type and chronicity type. 4)The disease index and lesion type of blade leaf of Pyricularia oryzae strains did not have consistent along the leaf position.

우리나라 벼·밀 및 콩 種子에 發生하는 傳染性 眞菌調査

白壽鳳 건국대학교 1982 學術誌 Vol.26 No.2

Detection and percentage infection on seed-borne fungi of rice, wheat and soybean in Korea was investigated. The results were as follow: ◁표삽입(원본p339참조)▷

벼種子 傳染性 病原菌의 發生에 미치는 培養 方法 및 光源의 影響

白壽鳳 建國大學校附設 農業資源開發硏究所 1980 論文集 Vol.5 No.-

벼品種 豊玉에 寄生하는 중요한 種子傳染性 病原菌의 發生에 미치는 培養方法 및 光源의 영향을 調査한 結果를 보면 다음과 같다. 1) Blotter method에서는 8種의 種子傳染性 病原菌이 檢出되었으나 Agar plate method에서는 4種의 種子傳染性 病原菌만 檢出되었다. 2) 계속 暗黑處理에서는 5種의 種子傳染性 病原菌이 檢出되었으나 近紫外線과 暗黑 12時間 交互照射 및 晝光色光線과 暗黑 12時間 交互照射處理에서는 각각 8種의 種子傳染性 病原菌이 檢出되었다. 3) 도열병균(Pyricularia oryzae)은 Agar plate method 와 暗黑處理에서는 檢出되지 않았으며 近紫外線과 暗黑 12時間 交互照射處理가 晝光色光線과 暗黑 12時間 交互照射處理에 비하여 效果가 있었다. 4) 이삭마름병균(Trichoconis padwickii)도 暗黑에서는 檢出되지 않았으며 近紫外線과 暗黑 12時間 交互照射處理가 晝光色 光線과 暗黑 12時間 交互照射處理에 비하여 현저히 效果가 있었다. The effect of incubation method and light on the development of an important seed-borne fungi which are parasiting on Pung-ok, a variety of rice, was investigated. Two incubation methods, Blotter method and Agar plate method were used. Three light conditions, cycles of 12 hour light/12 hour darkness with near ultraviolet light, the same cycles with artificial daylight, and continuous darkness were given. The results were as follows: 1. Eight different species of seed-borne fungi were detected when incubated by Blotter method, while only four species were detected by Agar plate method. 2. Either when incubated under the cycles of 12 hour light/12 hour darkness with near ultraviolet or artificial daylight eight species of the fungi were detected, but in darkness only five species were found. 3. Pyricularia oryzae was not detected by Agar plate method and in continuous darkness. It was found when incubated under light and darkness. The near ultraviolet was more effective than the artificial day light. 4. Trichoconis padwickii was detected by Agar plate method. Otherwise, the fungi showed the same results as Pyricularia oryzae.

엽권미생물을 이용한 고추탄저병(Colletotrichum dematium)의 생물학적 방제

백수봉,김동우,오연선 건국대학교 1996 學術誌 Vol.40 No.2

고추탄저병 (Colletotrichum dematium)에 대한 생물학적 방제 능력을 가지는 엽권미생물을 선발하기 위하여 38과 87종의 약용식물로부터 243균주의 세균과 51균주의 사상균 그리고 30균주의 효모를 분리하여 이들에 대하여 항진균활성검정을 in vitro 및 in vivo에서 실시한 결과는 다음과 같다. 1) 분리된 엽권미생물중에서 PDA배지 대치배양에서 15mm이상의 생육저지대를 형성하는 4개의 엽권미생물이 선발되었다. 2) 선발된 4개의 세균균주 KB6, KB12, KB13 및 KBl4의 PDB 배양여액 중에서 KB12 균주가 균사생장 억제율이 가장 높았다. 3) 푸른고추에서는 KB12 간주의 액체배양 여액이 가장 방제효과가 컸고, 붉은고추에서는 모든 균주에서 방제효과가 떨어졌다. 4) 푸른고추에서는 KB12 균주의 cell처리가 가장 방제 효과가 컸고, 붉은고추에서는 모든 균주에서 방제 효과가 크게 나타났다. 5) 선발된 4개의 세균균주들은 모두 Bacillus subtilis로 동정되었다. For the selection of biocontol agents against red-pepper anthracnose (Colletotrichum dematium) 243 isolates of bacteria, 51 of fungi and 30 of yeasts were obtained from phyllospere of medicinal plants. Of isolated microorganisms, four bacterial isolates, KB6, KB12, KB13 and KB14 were highly antagonistic to C. dematium than the others in dual culture test on potato dextrose agar(PDA). Among four bacterial isolates, culture filtrate of the isolate KB12 showed the highest inhibition to C. dematium on PDA. Culture filtrate of the isolate KB12 showed high control effect on the green fruits, but the control activity of the all isolates was not observed on the red fruits. In the living bacterial cell test, the isolate KB12 showed the highest control effect C. dematium on the green fruits. And all the isolates showed control effect to the pathogen on the red fruits. In the biochemical test, all the active isolates were identified as Bacillus subtilis.

콩炭疽病菌 Colletorichum truncatum이 生成하는 代謝毒素에 대한 콩品種의 特異性

白壽鳳 建國大學校 附設 農業資源開發硏究所 1993 農資源開發論集 Vol.18 No.-

본 연구는 콩품종에 대하여 콩炭疽病菌 Colletotrichum truncatum의 病原性과 培養濾液의 植物毒性을 조사한 결과를 요약하면 다음과 같다. 1) 콩품종에 대한 炭疽病菌 C. truncatum의 病原性은 보광콩이 高度의 感受性이고 단엽콩과 황금콩은 弱한 感受性이었다. 2) 炭疽病菌 C. truncatum 培養濾液 原液을 잎에 처리하면 培養日數나 培養方法에 따른 독성 차이는 뚜렷하지 못하였고 콩품종에 대한 독성은 단엽콩이 다소 弱하게 나타냈다. 그리고 培養濾液 原液을 苗에 처리하면 단엽콩에서 10일 培養液에서만 약간의 시들음을 보였고 다른 모든 처리에서는 완전히 시들음을 보였다. 20일 培養液에 대한 濃度別 처리에서 10倍液은 품종별 독성차이는 볼 수 없었으나 1,000倍液에서는 단엽콩이 抵抗性이고 보관콩은 感受性이었다. 그리고 靜置培養이나 振?培養에서의 독성 차이는 뚜렷하지 못하였다. 種子發芽에 미치는 독성은 20일培養濾液 原液이 가장 독성이 강하였고 靜置培養이나 振 培養에서 독성 차이는 뚜렷하지 못하였다. 품종간에서는 보광콩과 장경콩이 發芽抑制率이 가장 높았고 단엽콩이 가장 낮았다. 3) 培養濾液에 대한 작물간 독성은 10倍液에서 감자가 시들음이 가장 빨리 일어났고 심했으며 고추도시들음이 심했다. 그러나 땅콩과 윈드콩은 4일이 경과해도 시들음이 증상이 나타나지 않았다. The experiment was carried out to evaluate the pathogenicity and toxicity of Collectotrichum truncatum. Pathogen of C. truncatum cultured with different method, shaking and non-shaking, and different period. Results obtained from the experiment are summarized as follows: 1) There was varietal differences in pathogenicity of C. truncatum. Bokwnangkong was highly susceptible but not in Danyeobkong and Hwangkumkong. 2) When the filtrate of C. truncatum treated on the leaf of soybean, disease syndrome developed by toxic substances was not differed with cultual method and period. Danyeobkong showed a less toxicity symptom compared with other soybean varieties. 3) When the filtrate treated at seedling stage, all varieties showed completely wilting regardless of cultural method and period except for the Danyeobkong was susceptible at 1000-diluted filtrate which cultured for 20 days. 4) Germination of soybean seeds was strongly inhibited by filtrate which cultured 20 days. Bokwangkong and Jangkyeongkong showed very poor germination rate but not in Danyeobkong. 5) Toxicity symptom in terms of wilting was also found in potato and red peper but not in peanut and windbean even 4 days after treatment.

農作物 病害防除를 爲한 拮抗植物의 探索

白壽鳳,都銀洙 建國大學校 附設 農業資源開發硏究所 1991 農資源開發論集 Vol.16 No.-

30科 51種의 藥用植物을 공시하여 P. capsici, R. solani, F. oxysporum, B. cinerea, B. dothidea 및 C. dematium에 대해 抗菌力을 검정한 결과는 다음과 같다. 1) P. capsici는 마늘(Allium sativum), 지모(Anemarrhena asphodeloides), 대황(Rheum undulatum), 황백(Phellodendron amurense), 고본(Angelica tenuissina) 및 시호(Bupleurum falcatum) 등의 抽出物에 의해 菌絲生長이 저지되었다. 2)지모(Anemarrhena asphodeloides) 및 황백(Phellodendron amurense)의 抽出物이 F. oxysporum의 菌絲生長을 저지하였다. 3) C. dematium은 지모(Anemarrhena asphodeloides)와 고본(Anegelica tenuissina) 등의 抽出物에 의해 菌絲生長이 저지되었다. 4) 황백(Phellodendron amurense), 지모(Anemarrhena asphodeloides) 및 마늘(Allium sativum)은 각각 B. cinerea, B. dothidea 및 R. solani의 菌絲生長을 저지하는 효과가 있다. The extracts of medecinal plants of 51 species in 30 families were tested for antifungal activity against P. capsici, R. solani, F. oxysporum, B. cinerea, B. dothidea and C. dematium. The results were as follows : 1. The extracts of Allium sativum, Anemarrhena asphodeloides. Rheum undelatum, Phellodendron amurense, Angelica tenuissina and Bupleurum falcatum were strongly inhibitory against mycelial growth of P. capsici. 2. The extracts of Anemarrhena asphodeloides and Phellodendron amurense were strongly inhibitory against mycelial growth of F. oxysporum. 3. Mycelial growth of C. dematium was inhibited by the extracts of Anemarrhena asphodeloides and Angelica tenuissina. 4. The extracts of Phellodendron amurense, Anemarrhena asphodeloides and Allium sativum were inhibitory against mycelial growth of B. cinerea, B. dothidea, and R. solani, respectively.

수확후 채소류에 발생하는 진균독소의 탐색과 방제(Ⅱ) 이병된 양념 채소류(양파, 마늘, 고추)에서 주요 진균독소 검출

정일민,주호종,심성철,백수봉,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 Alternaria, Penicillium 및 Fusarium에 이병된 고추, 양파, 마늘을 HPLC로 주요독소들을 검정한 결과 고추에서 Alternaria 독소의 AOH(alternariol)는 소량∼440㎍/g 및 ALT(altenuene)는 소량∼103㎍/g, TeA(tenuagonic acid)는 249∼342㎍/g 및 AME(alternariol monomethyl ether)는 206∼294㎍/g이 검출되었고 양파, 마늘에서 Penicillium 독소의 citrinin이 2.8∼18.4㎍/g, penicillin-G는 0∼439.0㎍/g, penicillic acid는 0∼10.2㎍/g 및 patulin은 0∼7.0㎍/g 검출되었다. 그리고 양파, 마늘에서 Fusarium 독소로는 fusaric acid가 0∼553.6㎍/g 검출되었을 뿐 deoxynivalenol과 nivalenol은 검출되지 않았다. The major mycotoxins were detected from peppers, onions and garlics infected postharvest pathogens, Alternaria, Penicillium and Fusarium. Analyses of the major mycotoxins were conducted using HPLC. Detected Alternaria mycotoxins per gram of infected postharvest peppers were alternariol (AOH) with amount ranged from small quantity to 440 ㎍/g, altenuene (ALT) with amount ranged from small quantity to 103 ㎍/g, tenuagonic acid (TeA) with amount ranged from 249 to 342 ㎍/g and alternariol monomethyl ether (AME) with amount ranged from 206 to 294 ㎍/g. Penicillium toxins per gram of infected postharvest onions and garlics were citrinin with amount ranged from 2.8 to 18.4 ㎍/g, penicillun-G with amount ranged from no detection to 439.0 ㎍/g, penicillic acid with amount ranged from no detection to small quantity and patulin with amount ranged from no detection to small quantity. Fusarium toxins per gram of infected postharvest onions and garlics were fusaric acid with amount ranged from no detection to 553.6 ㎍/g. However, deoxyrivalenol and nivalenol were not detected from onins and garlics infected by Fusarium.

수확 후 과실류에 발생하는 진균독소의 탐색 및 방제 ; 2. 사과 , 배 , 귤 , 포도의 저장 중에 발생하는 Penicillium 독소 검출과 방제

백수봉(Su Bong Paik),정일민(Il Min Chung),유승헌(Seung Hun Yu),김은영(Eun Yong Kim) 한국균학회 2000 韓國菌學會誌 Vol.28 No.1

N/A

토양중의 Phytophthora spp. 방제를 위한 길항식물의 탐색

백수봉 ( Su Bong Paik ) 한국균학회 1989 韓國菌學會誌 Vol.17 No.1

수확후 곡물류에 발생하는 진균독소의 탐색과 방제 : Ⅲ. 수확후 곡물류(밀, 콩, 옥수수)에서 발생하는 진균독소균의 방제 Ⅲ. Control of Mycotoxin Producing Pathogens in Postharvest Cereals(Wheat, Bean, Corn)

백수봉,김은영,정일민,유승헌 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

수확후 곡물류에 발생하는 진균독소균에 의한 오염방제를 위하여 chitosan, grape fruit seed extract(GFSE), 및 sodium hypochloride gas 처리 효과를 검정하였다. 수확후 자연상태의 밀, 콩, 옥수수에 대한 모든 진균 오염방제효과는 sodium hypochloride gas 처리가 가장 컸고 GFSE 처리도 다소 효과가 있었으나 chitosan 처리는 효과가 없었다. 진균독소균 Penicillium spp. Aspergillus spp. 및 Fusarium spp.에 대한 방제 있어서도 sodium hypochloride gas의 처리 효과가 컸고, GFSE 처리도 다소 효과가 있었다. Sodium hypochloride gas의 노출시간은 24시간 이상 처리하면 효과가 있었다. This study was conducted to test the effect of chitosan, grape fruit seed extracts(GFSE) and sodium hypochloride gas on the control of mycotoxin producing pathogens occurred in postharvest grains. Among the treatments, sodium hypochloride gas showed the highest control effect on wheat, soybean and corn seed maintained in natural conditions after postharvest and GFSE had a little control effect, but chitosan treatment had no effect. Sodium hypochloride gas exhibited the strongest control effect on the major mycotoxin producing pathogens such as Penicillium spp. Aspergillus spp. and Fusarium spp., whereas GFSE had a little control effect. Sodium hypochloride gas appeared to be effective when the grains were treated with this gas more than 24 hours.