NST 통합감사와 출연연의 향방!

주호종 한국기술혁신학회 2019 한국기술혁신학회 학술대회 발표논문집 Vol.2019 No.11

Direct Evidence of Endophyte (Neotyphodium coenophialum) Genotype Effect on Growth and Vertical Transmission of Endophyte in Tall Fescue (Schedonorus phoenix Scop.) Under Water Stress

주호종 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.3

Tall fescue (Schedonorus phoenix Scop.) is resistant to abiotic and biotic stresses through a symbiotic relationship with Neotyphodium coenophialum. However, this endophyte has been considered detrimental since it produces toxic alkaloids to animals. It is vital to understand mutuality between these two to maximize positive impact of the endophyte on agri-ecosystem. Little research has been conducted on endophyte transmission mechanism in planta. To provide basic information related to endophyte transmission, an experiment was conducted to examine the effect of endophyte genotype and water stress on endophyte transmission by imposing soil moisture deficits at different stages of panicle development. There was water stress effect on endophyte frequency but not on concentration, whereas endophyte genotype significantly influenced endophyte concentration in pseudostem of tall fescue at boot stage. Reproductive tillers showed greater endophyte frequency and concentration. Endophyte frequency in florets or seeds depended on position within panicle. There was no drought effect on endophyte concentration, but showed the effect of endophyte genotype on endophyte concentration in florets and seeds. Overall endophyte concentration in seeds was higher. From this study, we may conclude that although water stress reduced endophyte frequency in vegetative tiller, water stress does not have effect on endophyte transmission, suggesting that drought is not an important factor controlling the endophyte transmission from plant to seed. Endophyte genotype and seed position in a panicle affected endophyte transmission,indicating that these two factors are involved in endophyte transmission and may determine seed transmission of endophyte in tall fescue.

Simple and Rapid Detection of Potato leafroll virus by Reverse Transcription Loop-mediated Isothermal Amplification

주호종 한국식물병리학회 2011 Plant Pathology Journal Vol.27 No.4

A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RTLAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter,the RT-LAMP (with loop primers) detects PLRV in less than 30 min, compared to 120 min of RT-PCR. By adding fluorescent reagent during the reaction, final products of the RT-LAMP were fluorescently visualized under UV light or could be differentiated by naked-eye inspection under normal light. The RT-LAMP was extremely sensitive, about 2000-fold more sensitive than RT-PCR. This study presents great potential of the RTLAMP for diagnosis and PLRV epidemiology because RT-LAMP method is speedy, sensitive, inexpensive, and convenient.

수박의 세균성과실썩음병(Bacterial Fruit Blotch)

주호종 한국종자연구회 2017 종자과학과 산업 Vol.13 No.1

벼 종실의 착립위치에 따른 미질 변이

안종국,정일민,주호종 建國大學校 附設 農業資源開發硏究所 1997 農資源開發論集 Vol.19 No.-

ABSTRACT Experiment was conducted to investigate the variation of grain quality in a panicle at experimental farm and laboratory of Kon Kuk University from April 1996 to March 1997.Spikelet number on the primary branch was ranged 5 to 6. However, spikelet number on the secondary branch was ranged 3 to 4.Total spikelet number on primary branches in a panicle was about 50 % in Hwaryeongbyeo and Chucheongbyeo. But it was only 35 % in Dasanbyeo which had a lot of secondary branches.Grain weight in the secondary branches were lighter than those in the primary branches. Protein content of milled rice in the primary branches was lower than that of milled rice in secondary branches, while amylose content of milled rice in primary branches was higher than that of milled rice in secondary branches. Protein content of milled rice on the upper part of branches was lower than that on the lower part of branches, while amylose content of milled rice on the upper part of branch was higher than that on the lower part branches in Chucheongbyeo. Fat acidity of rice flour on the primary branches showed lower value than on the secondary branches. Iodine blue value was the same as fat acidity value. Hardness of brown rice on the primary branches was stronger than on the secondary branches.

스마트 팜 실현을 위한 온실 온도관리 모델 평가와 저온기 근권가온 시나리오

주호종 ( Ho-jong Ju ) 전북대학교 농업과학기술연구소 2017 농업생명과학연구 Vol.48 No.2

현재 한국의 농업은 많은 문제에 직면 해있다. 가장 심각한 문제는 농가 인구의 지속적인 감소와 농부 노후화의 가속화 때문에 노동력 부족이다. 따라서 한국의 농산업은 다른 산업이나 다른 나라의 농산업에 비하여 매우 낮은 경쟁력을 가지고 있고 거의 고사 위기에 있다. 정부는 농업부문의 경쟁력 강화를 위해 많은 해결책과 정책들을 제시하였다. 정보 통신 기술 (ICT)과 융합 된 원예 시설 온실이 현대화 된 스마트 농장을 통해 원예 작물의 생산성을 높이고자 한다. 농업에 이 새로운 기술을 적용하는 것은 농업이 가진 여러 당면 문제를 해결할 수 있는 방안이 될 수 있다. 하지만 스마트 팜을 확산시키기 위해서는 더 많은 연구와 정부 지원이 필요한 상태이다. 여기서 저자는 스마트 팜과 연관된 사항들을 검토하고, 스마트 팜 확산이 일환으로 저온기 스마트 팜에 적용하여 근권부난방의 효율성을 증개시키고 작물의 생산성 증대를 위한 온도 관리 시나리오를 제안하고자 한다. Korea's agricultural development is facing with many problems. The most serious one is labor shortage because of the continuous decrease in farm population and the acceleration of agricultural workers’ aging. Therefore, Korea's agricultural industry has very low competitiveness and almost endangered compared to other industries in Korea and even compared to agricultural industry in other countries. Korea government proposed many solutions and policies to strengthen the competitiveness in agricultural sector. The government has planned to increase the productivity of horticulture crop through Smart farm which is very modernized greenhouse of horticultural facilities fused with Information and Communications Technologies(ICT). The adoption of this new technology in agriculture may be the solution for the problems that agriculture had. However, it needs more studies and much more government support to spread Smart farm. Here, author review the issues related to Smart farms and I propose the temperature management scenario to increase the efficiency of root heating and crop productivity by applying to Smart farm during low temperature season.

파프리카에 발생하는 주요 병원균에 대한 길항미생물, Serratia marcescens-YJK1, 분리와 특성

양수정,김형무,주호종 한국유기농업학회 2014 韓國有機農業學會誌 Vol.22 No.4

파프리카에 발생하는 병들을 방제하기 위하여 합성농약이 광범위하게 사용되어왔지만 최근에 수많은 농약사용의 부작용에 대한 관심이 증가 하고 있다. 파프리카 주요병인 잿빛곰팡이병, 줄기 및 과실썩음병, 역병, 균핵병, 시들음병을 방제하기 위한 미생물을 분리하고 특성을 파악하기 위하여 본 연구를 실시하였다. 지방산분석과 16S rDNA 염기배열은 이 연구에서 분리한 YKJ1가 Serratia marcescens 그룹에 속하는 것을 밝혔다. 특히, YKJ1의 16S rDNA 염기배열은 S. marcescens의 염기서열과 99% 상동성을 보였다. 광학현미경을 통해 YKJ1처리에 의해 병원균의 포자 발아 및 균사 생장이 저해됨을 확인 하였다. YKJ1처리는 팽윤균사와 같은 현저한 형태적 변화와 세포벽의 분해를 유발하였다. 역병균의 경우 유주자낭의 형성이 억제되었다. 본 연구에서 동정한 S. marcescens는 S. marcescens-YKJ1으로 부르고자 한다. 포장실험등과 같은 시험이 차후 더 요구되어지나 파프리카의 주요 병관리를 위한 생물적 방제제의 하나로 가치가 있을 것으로 생각된다. Synthetic agro-chemicals have been widely used to control diseases on paprika but these days negative attention has been increasing to use of them because of several adverse effects. This research was conducted to isolate and to characterize the antagonistic microorganism to control major paprika diseases, gray mold rot, fruit and stem rot, phytophthora blight, sclerotium rot, and wilt disease. Analysis of the fatty acid and analysis of the 16S rDNA gene sequence revealed that YKJ1 isolated in this research belongs to a group of Serratia marcescens. Specially, 16S rDNA gene sequence of YKJ1 showed 99% of sequence similarity with S. marcescens. Observation through the optical microscope revealed that YKJ1 suppressed the spore germination and the hyphal growth of pathogens. YKJ1 treatment on pathogens induced marked morphological changes like hyphal swelling and degradation of cell wall. In the case of phytophthora blight, the zoosporangium formation was restrained. S. marcescens found in this study call as S. marcescens-YKJ1 and it may be valuable as one of biological control agents against major diseases of paprika in the future even though it is require to be tested with more study on field test

2006~2010년도 국내 노지재배 고추의 바이러스병 발생현황

이장하,홍진성,주호종,박덕환 한국유기농업학회 2015 韓國有機農業學會誌 Vol.23 No.1

2006년부터 2010년까지 국내 고추 노지재배 포장을 대상으로 경상남도와 제주도를 제외한 강원도 11개 지역 등 전국에 걸쳐 총 83개 지역을 중심으로 바이러스 이병체를 채집하여 연차별 고추 발생 바이러스의 발병률, 바이러스 종류 및 감염 형태를 조사하였다. 2006년부터 2010년까지의 고추에서의 평균 단독 감염률은 46.6%, 복합 감염률은 48.0%로 조사되어 고추 생산에 있어서 가장 큰 위해요소는 바이러스 발병으로 나타났다. 바이러스별로는 CMV가 평균 34.8% 발병률로 가장 우점 바이러스로 조사되었다. 반면 BBWV2는 낮은 발병율을 보여주었으며, TSWV는 충남과 전남지역에 국한되는 것으로 조사되었다. 따라서 고추 노지재배 시 발생하는 바이러스의 위해도를 발병률에 따라 구분하면 CMV, PMMoV, 그리고 PepMov 순으로 조사되었다. 또한 복합 감염의 발생도 모든 조사지역 시료에서 검출되었으며, 이에 대한 감염 별 바이러스 종류와 발생률을 분석하였다. 복합 감염에서 가장 빈번하게 검출된 바이러스는 CMV로, CMV+BBWV2, CMV+PepMoV, CMV+PMMoV의 복합 감염형태가 검출률이 높게 나타났다. 따라서 고추의 유기농 재배·생산에 가장 큰 위협요인으로 작용하는 바이러스의 적절한 대책마련을 위한 지속적인 바이러스 모니터링과 3종 이상의 복합 감염 위해성을 판단하기 위한 추가 연구가 필요하다고 사료된다. In this study, viral disease samples were obtained between 2006 and 2010 from pepper fields in 11 major pepper-growing districts in Gangwon-do, and in 83 areas from other provinces, with the exception of Gyeongsangnam-do and Jeju island in Korea. In order to assess the type of infection, field surveys were conducted with regard to viral disease severity and virus type, based on typical symptoms on leaves. The means of single and mixed-virus infections were 46.6% and 48.0%, respectively, during those periods, suggesting that viruses are the agents that most severely decrease pepper production in field cultivation in Korea. In terms of single infection, Cucumber mosaic virus (CMV) was the most prevalent virus based on its disease severity ratings (34.8%). Next, Pepper mild mottle virus (PMMoV) and Pepper mottle virus (PepMoV) were shown to cause severe viral diseases in pepper, with disease severities of around 5-10%. On the other hand, Tomato spotted wilt virus (TSWV) occurs in a limited area in Chungcheongnam-do and Jeollanam-do. Thus, the viral disease caused by CMV, PMMoV, and PepMoV in pepper can be severe, and these virus types should remain considered critical reasons for decreased pepper production in field cultivation in Korea. In addition to single infection, mixed infections are frequently observed in collected pepper samples from all areas. The ratios of mixed infection were therefore studied to evaluate the disease severity of mixed infections and to define individual virus types. These data showed that different types of viruses were present, and CMV was the most abundant virus for mixed infection, as in the case of single infection. Among mixed infections, the highest disease severity was seen with CMV+Broad beam wilt virus 2 (BBWV2), followed by other types of mixed infection such as CMV+PepMoV and CMV+PMMoV. However, further work is needed to reduce the severe damage caused by viruses and to assess mixed infection types involving three or more viruses.

곤충병원성 곰팡이의 열안정성 향상을 위한 배지의 최적화

유정선,이세진,김시현,Yu-Shin Nai,주호종,김재수 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

곤충 병원성 곰팡이 Metharizium anisopilae JEF 003, 004와 Beauveria bassiana JEF 006, 007의 대량생산배지 조건에 따른 열안정성을 평가하였다. 첫 번째로 millet 배지에서 배양된 포자의 열안정성 평가를 위하여 50℃ 조건에 0, 30, 60, 90, 120 min으로 포자 현탁액 상태와 grain상태로 노출한 결과 현탁액 상태에서 포자 의 열안정성이 더 많이 감소하는 것을 확인하였다. 다음으로 각기 다른 고체배지 (조: 1~5.×109 conidia/g, 수수: 1~2×109 conidia/g, 기장: 2~3×109 conidia/g) 조건에 서 생산된 포자의 열안정성 확인을 위하여 배양이 완료된 포자를 현탁액과 grain상 태에서 50℃ 조건에 0, 1, 2, 4, 8 hours동안 노출하여 열안정성을 평가하였다. 실험 결과 현탁액 상태보다 grain상태에서 포자의 열안정성이 더 높은 것을 확인하였으 며, 조 배지 조건에서 포자의 열안정성이 가장 높게 향상된 것을 확인하였다. 마지 막으로 포자의 열안정성의 추가 향상을 위하여 배양이 완료된 고체배지 포자에 cotton seed oil, coconut oil, soybean oil, castor oil, olive oil, mineral oil을 넣고, 5 0℃의 온도에 0, 1, 2, 4, 8 hours으로 열안정성을 평가하였다. 결과적으로 cotton seed oil, soybean oil, castor oil, olive oil을 처리한 포자에서 높은 열안정성을 확인 하였다. 따라서 곤충병원성 곰팡이의 열안정성 실험 결과로 확인 된 조를 이용하여 높은 열안전성을 확보할 수 있을 것으로 판단이 되며, 추가적인 열안정성 확보를 위 하여 식물성 오일을 제제에 이용할 수 있을 것으로 전망된다.

국내 남부지역의 맥류 바이러스병 발생 현황

배주영,김상민,강미형,김강민,이중환,주호종,김선림,이봉춘 한국유기농업학회 2015 韓國有機農業學會誌 Vol.23 No.4

Virus disease surveys on barley cultivation areas in Jeonnam ․ Jeonbuk ․ Gyungnam ․ Gyungbuk-do were conducted during 2014-2015. In this study, we detected Barley yellow mosaic virus (BaYMV), Barley mild mosaic virus (BaMMV) and Soil-borne wheat mosaic virus (SBWMV) by RT-PCR from barley leaves. These viruses are of great economic importance for wheat and barley, causing significant quantitative and qualitative losses in yield. The result of investigation showed that the field incidence of BaYMV in Buan, Gimje was more than 90% in 2014. The infection field rate of barley virus including Boseong, Gangjin, Haenam, Jangheung in Jeonnam was ≈ 30%. In 2014, double infections by BaYMV and BaMMV was detected in Boseong, Gangjin, Haenam and Jangheung. Only as a low rate BaYMV occurred in various fields of Jeonbuk in 2015. At the same time high infection field ratio of 70% was observed in Gunsan. Also in Yeonggwang was double infection of BaYMV and BaMMV. BaYMV occurred single infection has been confirmed in all of the study area of Gyungbuk and Gyungnam except for Goseong during the investigation period.