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Nydia Ávila-Vanzzini,Juan Francisco Fritche-Salazar,Nelva Marina Vázquez-Castro,Pedro Rivera-Lara,Oscar Pérez-Méndez,Humberto Martínez-Herrera,Mario Gómez-Sánchez,Alberto Aranda-Frausto,Héctor Herrera 한국심초음파학회 2016 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.24 No.4
Background: Severe aortic stenosis (AS), leads to pathological left ventricular remodeling that may worsen with concomitantoverweight and obesity (OW/O). Methods: We aimed to prospectively analyze the impact of OW/O on ventricular remodeling in severe AS, by evaluating thepercentage of intraendomyocardial fibrosis (PIEF) and the percentage of infiltrating intraendocardial lipid vacuoles (PIELV) andits relationship to global longitudinal strain (GLS) in patients with OW/O. Results: 44 patients with severe AS were included, 13 non-obese (29%) and 31 OW/O (71%), all of them with left ventricularejection fraction ≥ 55%. GLS was evaluated with 2D speckle tracking. During valve replacement, an endocardial biopsy wasobtained, where PIEF and PIELV were analyzed. Patients with higher PIEF and PIELV had greater body mass index (p < 0.0001)and worse GLS (p < 0.0053). A GLS cut-off point < -14% had a sensitivity of 75%, and a specificity of 92.8% to detect importantPIEF (AUC: 0.928, 95% confidence interval: 0.798–1.00). On multivariate analysis, OW/O and PIELV were independentlyassociated to the PIEF, and OW/O and PIEF were independently associated to GLS. A high correlation between the amount ofPIELV and PIEF were found. Conclusion: Patients with severe AS and OW/O have greater PIEF and PIELV, suggesting more pathological remodeling. GLS is useful to detect subclinical myocardial injury and is potentially useful for endomyocardial fibrosis detection. The presenceof higher PIELF may be a trigger factor for the development of intraendomyocardial fibrosis.
Vicente Pla-Martí,Jose Martín-Arévalo,Rosa Martí-Fernández,David Moro-Valdezate,Stephanie García-Botello,Alejandro Espí-Macías,Miguel Mínguez-Pérez,Maria Dolores Ruiz-Carmona,Jose Vicente Roig-Vila 대한대장항문학회 2022 Annals of Coloproctolgy Vol.38 No.1
Purpose: This study was performed to evaluate the long-term evolution of continence and patient’s quality of life after surgical treatment for obstetric fecal incontinence. Methods: A prospective longitudinal study was conducted including consecutive patients who underwent sphincteroplasty for severe obstetric fecal incontinence. The first phase analyzed changes in continence and impact on quality of life. The second phase studied the long-term evolution reevaluating the same group of patients 6 years later. Degree of fecal incontinence was calculated using the Cleveland Clinic Score (CCS). Quality of life assessment was carried out with the Fecal Incontinence Quality of Life scale. Results: Thirty-five patients with median age of 55 years (range, 28 to 73 years) completed the study. Phase 1 results: after a postoperative follow-up of 30 months (4 to 132 months), CCS had improved significantly from a preoperative of 15.7±3.1 to 6.1±5.0 (P<0.001). Phase 2 results: median follow-up in phase 2 was 110 months (76 to 204 months). The CCS lowered to 8.4±4.9 (P=0.04). There were no significant differences between phases 1 and 2 in terms of quality of life; lifestyle (3.47± 0.75 vs. 3.16±1.04), coping/behavior (3.13±0.83 vs. 2.80±1.09), depression/self-perception (3.65±0.80 vs. 3.32± 0.98), and embarrassment (3.32±0.90 vs. 3.12±1.00). Conclusion: Sphincteroplasty offers good short-medium term outcomes in continence and quality of life for obstetric fecal incontinence treatment. Functional clinical results deteriorate over time but did not impact on patients’ quality of life.
Saratale, Ganesh D,Kshirsagar, Siddheshwar D,Sampange, Vilas T,Saratale, Rijuta G,Oh, Sang-Eun,Govindwar, Sanjay P,Oh, Min-Kyu Humana Press 2014 Applied biochemistry and biotechnology Vol.174 No.8
<P>Phanerochaete chrysosporium was evaluated for cellulase and hemicellulase production using various agricultural wastes under solid state fermentation. Optimization of various environmental factors, type of substrate, and medium composition was systematically investigated to maximize the production of enzyme complex. Using grass powder as a carbon substrate, maximum activities of endoglucanase (188.66 U/gds), exoglucanase (24.22 U/gds), cellobiase (244.60 U/gds), filter paperase (FPU) (30.22 U/gds), glucoamylase (505.0 U/gds), and xylanase (427.0 U/gds) were produced under optimized conditions. The produced crude enzyme complex was employed for hydrolysis of untreated and mild acid pretreated rice husk. The maximum amount of reducing sugar released from enzyme treated rice husk was 485 mg/g of the substrate. Finally, the hydrolysates of rice husk were used for hydrogen production by Clostridium beijerinckii. The maximum cumulative H2 production and H2 yield were 237.97 mL and 2.93 mmoL H2/g of reducing sugar, (or 2.63 mmoL H2/g of cellulose), respectively. Biohydrogen production performance obtained from this work is better than most of the reported results from relevant studies. The present study revealed the cost-effective process combining cellulolytic enzymes production under solid state fermentation (SSF) and the conversion of agro-industrial residues into renewable energy resources.</P>
N. Lopes,P. Piloto,P.M.M. Vila Real,L. Simões da Silva,J.-M. Franssen 국제구조공학회 2003 Steel and Composite Structures, An International J Vol.3 No.6
Two new formulae for the design of beam-columns at room temperature have been proposed into Eurocode 3, prEN 1993-1-1 (2002), and are the result of great efforts made by two working groups that followed different approaches, a French-Belgian team and an Austrian-German one. Under fire conditions the prEN 1993-1-2 (structural fire design) presents formulae, for the design of beam-columns based on the prENV 1993-1-1 (1992). In order to study the possibility of having, in part 1-1 and part 1-2 of the Eurocode 3, the same approach, a numerical research was made using the finite element program SAFIR, developed at the University of Liege for the study of structures subjected to fire.
Moisé,s Roberto Vallejo Pé,rez,Hugo Ricardo Navarro Contreras,Jesú,s A. Sosa Herrera,José,Pablo Lara Á,vila,Hugo Magdaleno Ramí,rez Tobí,as,Fernando Dí,az-Barriga Mart& 한국식물병리학회 2018 Plant Pathology Journal Vol.34 No.5
Clavibacter michiganensis subsp. michiganesis (Cmm) is a quarantine-worthy pest in México. The implementation and validation of new technologies is necessary to reduce the time for bacterial detection in laboratory conditions and Raman spectroscopy is an ambitious technology that has all of the features needed to characterize and identify bacteria. Under controlled conditions a contagion process was induced with Cmm, the disease epidemiology was monitored. Micro-Raman spectroscopy (532 nm λ laser) technique was evaluated its performance at assisting on Cmm detection through its characteristic Raman spectrum fingerprint. Our experiment was conducted with tomato plants in a completely randomized block experimental design (13 plants × 4 rows). The Cmm infection was confirmed by 16S rDNA and plants showed symptoms from 48 to 72 h after inoculation, the evolution of the incidence and severity on plant population varied over time and it kept an aggregated spatial pattern. The contagion process reached 79% just 24 days after the epidemic was induced. Micro-Raman spectroscopy proved its speed, efficiency and usefulness as a non-destructive method for the preliminary detection of Cmm. Carotenoid specific bands with wavelengths at 1146 and 1510 cm-1 were the distinguishable markers. Chemometric analyses showed the best performance by the implementation of PCA-LDA supervised classification algorithms applied over Raman spectrum data with 100% of performance in metrics of classifiers (sensitivity, specificity, accuracy, negative and positive predictive value) that allowed us to differentiate Cmm from other endophytic bacteria (Bacillus and Pantoea). The unsupervised KMeans algorithm showed good performance (100, 96, 98, 91 y 100%, respectively).
Clarissa A. Borges,Marita V. Cardozo,Livia G. Beraldo,Elisabete S. Oliveira,Renato P. Maluta,Kaline B. Barboza,Karin Werther,Fernando A. Ávila 한국미생물학회 2017 The journal of microbiology Vol.55 No.5
In order to describe the role of wild birds and pigeons in thetransmission of shiga toxigenic Escherichia coli (STEC) andenteropathogenic Escherichia coli (EPEC) to humans and otheranimals, samples were collected from cloacae and oropharynxof free-living wild birds and free-living pigeons. Two STEC(0.8%) and five EPEC strains (2.0%) were isolated from wildbirds and four EPEC strains (2.0%) were recovered from pigeons. Serogroups, sequence types (STs) and virulence genes,such as saa, iha, lpfAO113, ehxA, espA, nleB and nleE, detectedin this study had already been implicated in human and animaldiseases. Multidrug resistance (MDR) was found in 25.0%of the pigeon strains and in 57.0% of the wild bird strains;the wild birds also yielded one isolate carrying extended-spectrumβ-lactamases (ESBLs) gene blaCTX-M-8. The high variabilityshown by PFGE demonstrates that there are no prevalentE. coli clones from these avian hosts. Wild birds and pigeonscould act as carriers of multidrug-resistant STEC andEPEC and therefore may constitute a considerable hazard tohuman and animal health by transmission of these strainsto the environment.
Selection of Field Maize Hybrids for Adaptation to Southern Asia
Si-Hwan Ryu,Young-Ho Seo,Jae-Keun Choi,Jong-Yeol Park,Woo-Sik Yong,Sang-Min Lee,Ki-Jin Park,Vilas Chidley,Seung-Kyun Yang,Kyung-Hee Kim 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
With the economy development, people have an increasing demand for meat and eggs. Hence the market demand for cereal crop keeps increasing. maize is the major crop to meet the conditions of market demand. As a part of the Golden Seed Project, we are planning to develop maize varieties for adaptation in Southern Asia, especially in India. This project was started at 2013 and has been collaborated with Nongwoo Bio Co. Our project is based on overseas maize breeding in Bangalore, India. At first year, we tested 40 maize hybrids to investigate adaptability, yield capacity, and growing characteristics. Planting materials for this test were developed and selected in Cambodia by researchers of Maize Research Institute. Growing performance is usually quite different by the growing area and seasons, but some of our materials have shown good adaptability and yield at dry season in Southern India. 13IN07 was selected by yield and grain color, 13IN39 was selected by yield and plant type. These two selected hybrids were applied to regional test controled by Indian Council of Agricultural Research in India. We will see how our hybrids adapt in various regions in India, assess how other tested hybrids perform in those regions, and compare each other. We may also expect one of the selected hybrids can be a candidate variety for some regions in India and we will continuously select good hybrids and develop new varieties in India.