Characterization of Zinc-Solubilizing Bacillus Isolates and their Potential to Influence Zinc Assimilation in Soybean Seeds

( Sharma Sushil K. ),( Mahaveer P. Sharma ),( Aketi Ramesh ),( Om P. Joshi ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.3

One hundred thirty-four putative Bacillus isolates were recovered from soybean rhizosphere soils of Nimar region to select effective zinc solubilizers for increased assimilation of zinc (Zn) in soybean seeds. These isolates were screened in vitro for zinc-solubilization ability on Tris-minimal agar medium supplemented separately with 0.1% zinc in the form of zinc oxide, zinc phosphate, and zinc carbonate. Of all, 9 isolates and a reference Bacillus cereus ATCC 13061 were characterized and identified as Bacillus species based on Gram-positive reaction, endospore-forming cells, and the presence of iso-C15:0 and anteiso-C15:0 as predominant fatty acids. On plate assay, two isolates KHBD-6 and KHBAR-1 showed a greater diameter of solubilization halo and colony diameter on all the three zinc compounds. The isolates KHBD-6, KHBAR-1, BDSD-2-2C, and KHTH-4-1 and the reference strain ATCC 13061 had higher soluble zinc concentration in liquid medium supplemented with zinc phosphate and zinc carbonate compounds as compared with the other isolates and uninoculated control. Evaluation under microcosm conditions showed that inoculation of isolates KHBD-6 (57.34 μg/g), KHBAR-1 (55.67 μg/g), and strain ATCC 13061 (53.10 μg/g) significantly increased the Zn concentration in soybean seeds as compared with the other isolates and uninoculated control (47.14 μg/g). This study suggests the occurrence of zinc-solubilizing Bacillus in soils of Nimar region and isolates KHBD-6 and KHBAR-1 were found to be promising zinc solubilizers for increased assimilation of Zn in soybean seeds.

Plant Diversity, Tree Regeneration, Biomass Production and Carbon Storage in Different Oak Forests on Ridge Tops of Garhwal Himalaya

Sharma, Chandra Mohan,Tiwari, Om Prakash,Rana, Yashwant Singh,Krishan, Ram,Mishra, Ashish Kumar Institute of Forest Science 2016 Journal of Forest Science Vol.32 No.4

The present study was conducted on ridge tops of moist temperate Oak forests in Garhwal Himalaya to assess the plant diversity, regeneration, biomass production and carbon assimilation in different Oak forests. For this purpose, three Oak forest types viz., (a) Quercus leucotrichophora or Banj Oak (FT1; between 1,428-2,578 m asl), (b) Quercus floribunda or Moru Oak (FT2; between 2,430-2,697 m asl) and (c) Quercus semecarpifolia or Kharsu Oak (FT3; between 2,418-3,540 m asl) were selected on different ridge tops in Bhagirathi catchment area of Garhwal Himalaya. A total of 91 plant species including 23 trees (8 gymnosperms and 15 angiosperms), 21 shrubs and 47 herbs species belonging to 46 families were recorded from all the ridge top Oak forests. The highest mean tree density ($607{\pm}33.60trees\;ha^{-1}$) was observed in Q. floribunda forest with lower mean total basal cover (TBC) value ($48.02{\pm}3.67m^2ha^{-1}$), whereas highest TBC value ($80.16{\pm}3.30m^2ha^{-1}$) was recorded for Q. semecarpifolia forest, with lowest mean stem density ($594{\pm}23.43stems\;ha^{-1}$). The total biomass density (TBD) across three Oak forests ranged between $497.32{\pm}83.70$ (FT1) and $663.16{\pm}93.85t\;ha^{-1}$ (FT3), while the total carbon density (TCD) values ranged between $228.75{\pm}22.27$ (FT1) and $304.31{\pm}18.12t\;ha^{-1}$ (FT3). Most of the tree species were found with good regeneration (GR) status (average 45%) in all the forest types whereas, few species were found not regenerating (NR) (average 17%) however, few new recruitments were also recorded. ANOVA (Post-Hoc Tukey's test at 5% significance level) indicated significant forest-wise differences in TBC, TBD and TCD (in tree layer); family and evenness (in shrub layer only) values, while insignificant differences were noticed in density values of tree, seedling and herb layer.

Disturbance, Diversity, Regeneration and Composition in Temperate Forests of Western Himalaya, India

Om Prakash Tiwari,Chandra Mohan Sharma,Yashwant Singh Rana,Ram Krishan 강원대학교 산림과학연구소 2019 Journal of Forest Science Vol.35 No.1

We have investigated the impact of anthropogenic and natural disturbances on regeneration, composition and diversity in some temperate forests of Bhagirathi Catchment Area of Garhwal Himalaya. The forests were categorized on the basis of canopy cover and magnitude of disturbance into highly, moderately and least disturbed classes. The dominant tree species at lower elevation were Pinus roxburghii and Quercus leucotrichophora, while Abies pindrow, Q. semecarpifolia and Rhododenron arboreum were the dominant species at the upper elevational forests. Cythula tomentosa and Indegophera heterentha were the dominant shrub species present in all the forests. Similarly, Circium wallichii and Oxalis corniculata were the dominant herb species found in all forests (except Q. leucotrichophora forest), whereas Thalictrum foliolosum and Viola pilosa were noticed in each forest (except P. roxburghii forest). The tree density values oscillated between 400±10 trees ha-1 to 750±89.1 trees ha-1 which generally decreased from lower to higher disturbance regimes however, the total basal cover value was highest (88.1±23.6 m2 ha-1) in highly disturbed forest and lowest (25.8±2.2 m2 ha-1) in moderately disturbed forest. The shrub and herb densities were maximum in least disturbed forest, while the young regenerating individuals i.e., sapling and seedling were observed increasing from high to low disturbed forests which reflected that the forest fragmentation adversely affected the regeneration. However, A. pindrow and P. roxburghii were found invariably encroaching the habitats of R. arboreum and Q. leucotrichophora at various altitudes, respectively. The Canonical Correspondence Analysis clearly indicated that the elevation and lopping intensity have more impact on trees, while shrub and herbs were more influenced by elevation, canopy cover, light attenuation and soil erosion. Pinus roxburghii was the only species which was affected by heavy litter removal and forest fire.

Regeneration dynamics, population structure, and forest composition in some ridge forests of the Western Himalaya, India

Om Prakash Tiwari,Yashwant Singh Rana,Ram Krishan,Chandra Mohan Sharma,Bhupendra Singh Bhandari 한국산림과학회 2018 Forest Science And Technology Vol.14 No.2

The present study aimed to analyze the regeneration dynamics, population structure, and forest composition in some ridge forests of the Western Himalaya to elucidate the impact of climate change. The highest species richness (trees, 17; saplings, 16; seedlings, 16) was recorded in mixed Rhododendron arboreum forest. The maximum tree density (737 § 25.93 trees ha¡1; mixed Quercus floribunda); sapling density (5342 § 587.54 saplings ha¡1; mixed Quercus semecarpifolia), and seedling density (71,429 § 26,632.29 seedlings ha¡1; mixed Cedrus deodara) were recorded in forests of higher altitudes. The mean basal cover values in these forests oscillated between 39.21 § 1.53 m2 ha¡1 (mixed Quercus leucotrichophora) to 87.65 § 15.45 m2 ha¡1 (mixed Abies pindrow). The highest Shannon index value (0.51 § 0.05) and species evenness (0.36 § 0.02) for trees were recorded in mixed Rhododendron arboreum forest. Some species like Betula utilis, Myrica esculenta, Ficus rumphii, Ilex dipyrena, Quercus floribunda, Litsea elongata, Symplocos paniculata, and Abies spectabilis were noticed as new recruiters (recent invaders) in new habitats, which may change the future forest composition. This study revealed that ridge forests of the Bhagirathi catchment area were in transition phase at middle altitudes. Mixed Quercus floribunda forest was observed to be the youngest regenerating forest; however, mixed Abies pindrow forest was the most mature old-growth forest.

EXISTENCE OF SOLUTION FOR SYSTEM OF INTEGRAL EQUATIONS AND FUNCTIONAL EQUATIONS THROUGH NON-NEGATIVE FUNCTION WITH PROPERTY OF ONENESS

Om Prakash Chauhan,Deepak Singh,Vishal Joshi,Garima Singh,Rajendra Kumar Sharma 경남대학교 수학교육과 2018 Nonlinear Functional Analysis and Applications Vol.23 No.4

In this article, we define the notion of a non-negative function satisfying property of oneness. Utilizing this concept, certain unified fixed point results are established. Furthermore generalized φ-contractions mapping and some fixed point results in the framework of symmetric space are also presented. Some well-known results from the existence literature are obtained through our introduced setting. Results are substantiated by some innovative examples with the aid of visualization of surfaces. Moreover applications of obtained theory for the existence for the system of integral equations as well as for the system of functional equations occurred in dynamic programming are also presented.

Some New Measures of Fuzzy Directed Divergence and Their Generalization

Om Parkash,P. K. Sharma 한국수학교육학회 2005 純粹 및 應用數學 Vol.12 No.4

There exist many measures of fuzzy directed divergence corresponding to the existing probabilistic measures. Some new measures of fuzzy divergence have been proposed which correspond to some well-known existing probabilistic measures. The essential properties of the proposed measures have been developed which contains many existing measures of fuzzy directed divergence.

Scan Range Analysis According to Variation of Einzel Lens Electrode Gap in Microcolumn

Suwal, Om Krishna,Sharma, Anjli,Kim, Dae-Wook,Oh, Tae-Sik,Kim, Ho-Seob,Kim, Young Jung,Kim, Young-Chul Chemic Publishing Company 2014 Asian Journal of Chemistry Vol.26 No.5

<P>Microcolumn is a miniaturized electron optical system having a miniaturized electron emitter, electrostatic source lenses, deflectors and an Einzel lens. The optimization of each components are still technically challenging for aberration-free e-beam operation, high quality imaging and large field of view. This paper discusses the influence of gap between Einzel lens electrodes on scan range and focusing nature in various modes of operation. In source lens focusing mode, the scan range is found to be increased with reducing the gap, but the result was opposite in two lens focusing mode where both source and Einzel lens were used for focusing the beam. We also proved these results through simulation analysis on electron beam trajectory and electric field.</P>

Liquid Phase Exfoliation and Characterization of Few Layer MoS2 and WS2 Nanosheets as Channel Material in Field Effect Transistor

Rohit Sharma,Ashish Kumar,Anit Dawar,Sunil Ojha,Ambuj Mishra,Anshu Goyal,Radhapiyari Laishram,V. G. Sathe,RITU SRIVASTAVA,Om Prakash Sinha 한국전기전자재료학회 2023 Transactions on Electrical and Electronic Material Vol.24 No.2

Field effect transistors (FETs) are considered as the backbone of electronic industry. In this study, we adopted a simple drop cast method for the fabrication of MoS2 and WS2 channel based FET on commercially available pre-patterned OFET devices. The synthesis of few-layers thick MoS2 and WS2 nanosheets (NSs) has been done by solvent-assisted exfoliation method. FESEM and TEM study reveals that NSs have lateral dimensions in micron and have polycrystalline nature. From XPS, it is observed that MoS2 NSs has 2H phase whereas WS2 have hybrid 1T and 2H phase. The frequency difference in Raman vibrational mode for MoS2 and WS2 NSs is 24.08 cm-1 and 63.84 cm-1 respectively, confirms that number of layers is reduced after sonication. UV-visible spectroscopy reveals that the bandgap is 1.7 eV and 1.8 eV for MoS2 and WS2 NSs respectively. Later, these nanosheets have been drop-casted as the channel material on pre-patterned FETs devices and their output and transfer characteristics have been studied. It found that the current On/Off ratio is 10 4 and 10 3 for MoS 2 and WS2-FET device respectively. This facile fabrication of FET devices may provide a new stage for researchers who do not have access of lithography facilities for FET fabrication.

On the Micchelli combinations of modified beta operators

Diwaker Sharma,Om Prakash,Prerna Maheshwari 장전수학회 2012 Proceedings of the Jangjeon mathematical society Vol.15 No.4

In the present paper, we study modified Beta operator with the weight function of Baskakov basis function. We extend the result of [3] and study simultaneous approximation properties of these operators, we estimate the local direct result in terms of modulus of continuity. Here we improve the order of approximation for these operators by applying iterative condition. We also establish an asymptotic formula and error estimate in terms of higher order modulus of continuity in simultaneous approximation for the iterative combinations (see also [9]) of these operators.

Disturbance, Diversity, Regeneration and Composition in Temperate Forests of Western Himalaya, India

Tiwari, Om Prakash,Sharma, Chandra Mohan,Rana, Yashwant Singh,Krishan, Ram Institute of Forest Science 2019 Journal of Forest Science Vol.35 No.1

We have investigated the impact of anthropogenic and natural disturbances on regeneration, composition and diversity in some temperate forests of Bhagirathi Catchment Area of Garhwal Himalaya. The forests were categorized on the basis of canopy cover and magnitude of disturbance into highly, moderately and least disturbed classes. The dominant tree species at lower elevation were Pinus roxburghii and Quercus leucotrichophora, while Abies pindrow, Q. semecarpifolia and Rhododenron arboreum were the dominant species at the upper elevational forests. Cythula tomentosa and Indegophera heterentha were the dominant shrub species present in all the forests. Similarly, Circium wallichii and Oxalis corniculata were the dominant herb species found in all forests (except Q. leucotrichophora forest), whereas Thalictrum foliolosum and Viola pilosa were noticed in each forest (except P. roxburghii forest). The tree density values oscillated between $400{\pm}10\;trees\;ha^{-1}$ to $750{\pm}89.1\;trees\;ha^{-1}$ which generally decreased from lower to higher disturbance regimes however, the total basal cover value was highest ($88.1{\pm}23.6m^2\;ha^{-1}$) in highly disturbed forest and lowest ($25.8{\pm}2.2m^2\;ha^{-1}$) in moderately disturbed forest. The shrub and herb densities were maximum in least disturbed forest, while the young regenerating individuals i.e., sapling and seedling were observed increasing from high to low disturbed forests which reflected that the forest fragmentation adversely affected the regeneration. However, A. pindrow and P. roxburghii were found invariably encroaching the habitats of R. arboreum and Q. leucotrichophora at various altitudes, respectively. The Canonical Correspondence Analysis clearly indicated that the elevation and lopping intensity have more impact on trees, while shrub and herbs were more influenced by elevation, canopy cover, light attenuation and soil erosion. Pinus roxburghii was the only species which was affected by heavy litter removal and forest fire.