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Inhibitory Activity of Isorhamnetin from Persicaria thunbergii on Farnesyl Protein Transferase
Oh Hyun Mi,Kwon Byoung-Mog,Baek Nam-In,Kim Sung-Hoon,Chung In-Sik,Park Mi-Hyun,Park Hee Wook,Lee Jae Hyeok,Park Hye Won,Kim Eun Jeong,Kim Dae Keun The Pharmaceutical Society of Korea 2005 Archives of Pharmacal Research Vol.28 No.2
The methanolic extract of the aerial parts of Persicaria thunbergii was found to show inhibitory activity on Farnesyl Protein Transferase (FPTase). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of isorhamnetin, as an inhibitor on FPTase. This compound inhibited FPTase activity in a dose-dependent manner, and the $IC_{50}$ value of isorhamnetin was $37.5\;{\mu}M$.
( Keun Young Shin ),( Jun Heo ),( Ji Yeon Kim ),( Sang Jik Lee ),( Se Young Jang ),( Soo Young Park ),( Min Kyu Jung ),( Chang Min Cho ),( Won Young Tak ),( Young Oh Kweon ) 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.2
Radiofrequency ablation (RFA) is performed as an alternative to surgical resection for primary or secondary liver malignancies. Although RFA can be performed safely in most patients, early and late complications related to mechanical or thermal damage occur in 8-9.5% cases. Hemocholecyst, which refers to hemorrhage of the gallbladder, has been reported with primary gallbladder disease or as a secondary event associated with hemobilia. Hemobilia, defined as hemorrhage in the biliary tract and most commonly associated with accidental or iatrogenic trauma, is a rare complication of RFA. Here we report a case of hemocholecyst associated with hemobilia after RFA for hepatocellular carcinoma that was successfully managed by laparoscopic cholecystectomy.
Oh Se-Kyung,Kim Won-Cheol,Ahn Soo-Whan,Kang Ho-Keun,Kim Myoung-Ho The Korean Society of Marine Engineering 2005 한국마린엔지니어링학회지 Vol.29 No.3
An experimental study on the heat transfer and friction characteristics of a fully developed turbulent air flow in a square channel with $45^{\circ}$ inclined ribs on one, two, and four walls is reported. Tests were performed for Reynolds number ranging from 7,600 to 24,900. The pitch-to-rib height ratio, p/e, was kept at 8 and rib height-to-channel hydraulic diameter ratio, $e/D_h$, was kept at 0.0667. The heat transfer coefficient and friction factor values were enhanced with the increase in the number of ribbed walls. Results of this investigation could be used in various applications of internal channel turbulent flows involving different number of roughened walls.
Oh, Hyung-Keun,Kim, Won-Kee,Kang, Ah-Rang,Kim, In-Seon,Lee, Hyang-Burm,Kim, Ik-Soo Korean Society of Sericultural Science 2010 International Journal of Industrial Entomology Vol.21 No.1
The perilla leaf pyralid moth, Pyrausta panopealis Walker (Lepidoptera: Pyralidae), is a serious pest damaging to leaf perilla. In order to establish the life parameters of P. panopealis for eventual purpose of control, the developmental span of each stage was investigated under five temperature regimes ($20^{\circ}C{\sim}30^{\circ}C$). The larval period of P. panopealis was longest as 26.8 days at $20^{\circ}C$ and shortened as temperature goes up to $30^{\circ}C$ as 11.3 days. Survivorship of the larval P. panopealis was the highest at $27.5^{\circ}C$ as 82.5%, whereas that of other temperatures ranged from 40% ($20^{\circ}C$) to 60.0% ($30^{\circ}C$), indicating that the P. panopealis appears to favor somewhat higher temperature. In addition to larval period, the duration of egg, prepupal, and pupal period also were shortened sharply as temperature goes up, whereas the duration of adult stage shortened a maximum of only two days as temperature goes up. After the perilla leaf pyralid moths were successfully stabilized in indoor environment the larvicidal efficacy of ten on-the-market environmentfriendly agricultural materials (EFAMs) that were previously selected from the result of other moth species was tested. Seven of the ten tested showed more than 90% of mortality within 12 hrs and reached nearly up to 100% within 24 hrs, but the remaining three showed less than ~70%.
Achieving tolerant CO<sub>2</sub> electro-reduction catalyst in real water matrix
Won, Da Hye,Shin, Hyeyoung,Chung, Min Wook,Jung, Hyejin,Chae, Keun Hwa,Oh, Hyung-Suk,Hwang, Yun Jeong,Min, Byoung Koun Elsevier 2019 Applied Catalysis B Vol.258 No.-
<P><B>Abstract</B></P> <P>In order to achieve practical application of electrochemical CO<SUB>2</SUB> conversion technologies, the development of durable catalyst in real water matrix is essential because the use of catalysts only showing high performance within a well-refined environment cannot guarantee their feasibility in realistic conditions. Here, we report a design strategy for a catalyst, which shows excellent tolerance to deactivation factors, using a carbon-based material under more practical condition implemented by real tap water. Screening analyses on various components in tap water elucidated that the impurity group, which can be deposited on the catalyst surface and impede the active sites, such as copper, zinc, and especially iron are the main factors responsible for deactivation. Based on these findings, the structural modified nitrogen-doped carbon nanotube (denoted as ball mill N-CNT) was adopted as a catalyst design to secure durability. Consequently, the ball mill N-CNT revealed tolerance to the disclosed deactivation factors and showed stable performance during unprecedented long-time of 120 h in tap water media.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Electro-reduction of CO<SUB>2</SUB> was performed in real water matrix (tap water) conditions. </LI> <LI> Iron was found to be the most detrimental element in silver catalyzed CO<SUB>2</SUB> electro-reduction. </LI> <LI> Nitrogen doped carbon based material was designed to be a stable model catalyst in real water. </LI> <LI> Stable performance was tested for an unprecedented long-time of 120 h in tap water media. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Surface Plasmon Resonance Immunosensor for Detection of Legionella pneumophila
Oh, Byung-Keun,Lee, Woochang,Bae, Young-Min,Lee, Won-Hong,Park, Jeong-Woo The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
An immunosensor based on surface plasmon resonance (SPR) onto a protein G layer by Self-assembly technique was developed for detection of Legionella pneumophila. The protein G layer by self-assembly technique was fabricated on a gold (Au) surface by adsorbing the 11-mercaptoundecanoic acid (MUA) and an activation process for the chemical binding of the free amino (-NH$_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDAC) in series. The formation of the protein G layer by self-assembly technique on the Au Substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The Surface topographies of the fabricated thin films on an Au substrate were also analyzed by using an atomic force microscope (AFM). Consequently, an immunosensor for the detection of L. pneumophila using SPR was developed with a detection limit of up to 10$^2$CFU per mL.
Oh, Byung-Keun,Lee, Woochang,Lee, Won-Hong,Park, Jeong-Woo The Korean Society for Biotechnology and Bioengine 2003 Biotechnology and Bioprocess Engineering Vol.8 No.4
A self-assembled monolayer of protein G was fabricated to develop an immunosensor based on surface plasmon resonance (SPR), thereby improving the performance of the antibodybased biosensor through immobilizing the antibody molecules (lgG). As such, 11-mercaptoundecanoic acid (11-MUA) was adsorbed on a gold (Au) support, while the non-reactive hydrophilic surface was changed through substituting the carboxylic acid group (-COOH) in the 11-MUA molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholide (EDAC). The formation of the self-assembled protein G layer on the Au substrate and binding of the antibody and antigen were investigated using SPR spectroscopy, while the surface topographies of the fabricated thin films were analyzed using atomic force microscopy (AFM). A fabricated monoclonal antibody (Mab) layer was applied for detecting E. coli O157:H7. As a result, a linear relationship was achieved between the pathogen concentration and the SPR angle shift, plus the detection limit was enhanced up to 10$^2$ CFU/mL.