Synthesis, Crystal Structure and Quantum Chemistry of a Novel Schiff Base N-(2,4-Dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine

Ning-Ning Ji,Zhi-Qiang Shi,Ren-Gao Zhao,Ze-Bao Zheng,Zhi-Feng Li 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.4

A novel Schiff base N-(2,4-dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine has been synthesized and structurally characterized by X-ray single crystal diffraction, elemental analysis, IR spectra and UV-vis spectrum. The crystal belongs to monoclinic with space group P21/n. The molecules are connected via intermolecular O-H···O hydrogen bonds into 1D infinite chains. The crystal structure is consolidated by the intramolecular N-H···O hydrogen bonds. weak intermolecular C-H···O hydrogen bonds link the molecules into intriguing 3D framework. Furthermore, Density functional theory (DFT) calculations of the structure, stabilities, orbital energies, composition characteristics of some frontier molecular orbitals and Mulliken charge distributions of the title compound were performed by means of Gaussian 03W package and taking B3LYP/6-31G(d) basis set. The time-dependent DFT calculations have been employed to calculate the electronic spectrum of the title compound, and the UV-vis spectra has been discussed on this basis. The results show that DFT method at B3LYP/6-31G(d) level can well reproduce the structure of the title compound.

Synthesis, Crystal Structure and Quantum Chemistry of a Novel Schiff Base N-(2,4-Dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine

Ji, Ning-Ning,Shi, Zhi-Qiang,Zhao, Ren-Gao,Zheng, Ze-Bao,Li, Zhi-Feng Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.4

A novel Schiff base N-(2,4-dinitro-phenyl)-N'-(1-phenyl-ethylidene)-hydrazine has been synthesized and structurally characterized by X-ray single crystal diffraction, elemental analysis, IR spectra and UV-vis spectrum. The crystal belongs to monoclinic with space group P21/n. The molecules are connected via intermolecular O-$H{\cdots}O$ hydrogen bonds into 1D infinite chains. The crystal structure is consolidated by the intramolecular N-$H{\cdots}O$ hydrogen bonds. weak intermolecular C-$H{\cdots}O$ hydrogen bonds link the molecules into intriguing 3D framework. Furthermore, Density functional theory (DFT) calculations of the structure, stabilities, orbital energies, composition characteristics of some frontier molecular orbitals and Mulliken charge distributions of the title compound were performed by means of Gaussian 03W package and taking B3LYP/6-31G(d) basis set. The time-dependent DFT calculations have been employed to calculate the electronic spectrum of the title compound, and the UV-vis spectra has been discussed on this basis. The results show that DFT method at B3LYP/6-31G(d) level can well reproduce the structure of the title compound.

Nepetin, a natural compound from Inulae flos, suppresses degranulation and eicosanoid generation through PLCγ1 and Akt signaling pathways in mast cells

Ning Ji,Sun‑Gun Kim,Hyo‑Hyun Park,Eujin Lee,이윤주,Meihua Jin,Eunkyung Lee 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.2

Nepetin derived from the flowers of Inulajaponica, Inulae flos, has been reported to exert several biologicalactivities, including anti-inflammatory responses. Inthis study, we evaluated the anti-allergic property of nepetinwith its molecular mechanisms in bone marrow-derived mastcells (BMMC) and mice. In this in vitro study, we investigatedthe inhibitory effects of nepetin on degranulation andgeneration of leukotriene C4(LTC4) and prostaglandin D2(PGD2) in IgE/antigen (Ag)-stimulated BMMC. The effectof nepetin on passive cutaneous anaphylaxis (PCA) reactionwas also studied in mice. Nepetin reduced degranulation andLTC4generation in BMMC. The IgE/Ag-mediated signalingpathway demonstrated that nepetin suppressed intracellularCa2+level and activation of PLCγ1 and cPLA2. However,MAPKs were not affected by nepetin in BMMC. In addition,nepetin treatment reduced PGD2production and suppressedcyclooxygenase-2 protein expression via the inhibition of theAkt and nuclear factor-κB signaling pathways. With respectto the local allergic response in vivo, oral administration ofnepetin suppressed mast cell-dependent PCA reaction in adose-dependent manner. The results of this study suggest that nepetin might have an anti-allergic potential related tomast cell-mediated inflammatory diseases.

Carbon-Ion-Implanted Nd, MgO-Codoped Near-Stoichiometric Lithium-Niobate Optical Waveguides

Ning-Ning Dong,Yang Tan,Yan-Xia Kong,Feng Chen,Hong-Ji Ma 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.6

Nd, MgO codoped near stoichiometric lithium niobate (Nd:MgO:SLN) is a promising laser gain crystal for infrared laser generations. We report on, to our knowledge for the first time, the planar waveguide formation in Nd:MgO:SLN crystal by MeV carbon ion implantation at dose of 1014 ions/cm2. The guiding properties are investigated by using the well-known m-line technique and end-face arrangement. The refractive index profiles of the waveguides show a typical “well + barrier” distribution, which could confine the light propagation in a non-leaky way. In addition, a numerical simulation of the light propagation shows that the waveguide modal distributions are in good agreement with the experimental data, which means potential applications for the formation of designable devices in Nd:MgO:SLN by using carbon ion implantation. Nd, MgO codoped near stoichiometric lithium niobate (Nd:MgO:SLN) is a promising laser gain crystal for infrared laser generations. We report on, to our knowledge for the first time, the planar waveguide formation in Nd:MgO:SLN crystal by MeV carbon ion implantation at dose of 1014 ions/cm2. The guiding properties are investigated by using the well-known m-line technique and end-face arrangement. The refractive index profiles of the waveguides show a typical “well + barrier” distribution, which could confine the light propagation in a non-leaky way. In addition, a numerical simulation of the light propagation shows that the waveguide modal distributions are in good agreement with the experimental data, which means potential applications for the formation of designable devices in Nd:MgO:SLN by using carbon ion implantation.

Ultra-precision machining of a large amplitude umbrella surface based on slow tool servo

Peixing Ning,Ji Zhao,Shijun Ji,Jingjin Li,Handa Dai 한국정밀공학회 2020 International Journal of Precision Engineering and Vol.21 No.11

Due to its excellent optical performance and system integration property, micro-nano structure function surface has been widely used in optical apparatus and energy collection equipment. But using traditional processing method can’t obtain a mirror-like surface with high form accuracy and low roughness for sake of its complex micro-nano structure. In this paper, a large amplitude umbrella surface is special designed and single point diamond turning (SPDT) technology based on slow tool servo (STS) is used for umbrella surface machining. The tool path generation, tool radius compensation and tool geometry optimization are detailed for fabricating the desired surface. A new method combined with constant angle and analytical mathematical optimization is proposed for tool path optimization. A large amplitude umbrella surface with the maximum amplitude 0.2 mm and period 8 per cycle is designed, and the machining experiment is carried out on Nanoform 250 ultra-precision machine tool. From the form accuracy 1 μm in surface residual error and 279.12 nm in RMS and the surface roughness 230.10 nm in Ra for the machined surface, it can be seen that a large amplitude umbrella surface is easily fabricated by SPDT under STS controlling.

SVM-Based Incremental Learning Algorithm for Large-Scale Data Stream in Cloud Computing

( Ning Wang ),( Yang Yang ),( Liyuan Feng ),( Zhenqiang Mi ),( Kun Meng ),( Qing Ji ) 한국인터넷정보학회 2014 KSII Transactions on Internet and Information Syst Vol.8 No.10

We have witnessed the rapid development of information technology in recent years. One of the key phenomena is the fast, near-exponential increase of data. Consequently, most of the traditional data classification methods fail to meet the dynamic and real-time demands of today`s data processing and analyzing needs--especially for continuous data streams. This paper proposes an improved incremental learning algorithm for a large-scale data stream, which is based on SVM (Support Vector Machine) and is named DS-IILS. The DS-IILS takes the load condition of the entire system and the node performance into consideration to improve efficiency. The threshold of the distance to the optimal separating hyperplane is given in the DS-IILS algorithm. The samples of the history sample set and the incremental sample set that are within the scope of the threshold are all reserved. These reserved samples are treated as the training sample set. To design a more accurate classifier, the effects of the data volumes of the history sample set and the incremental sample set are handled by weighted processing. Finally, the algorithm is implemented in a cloud computing system and is applied to study user behaviors. The results of the experiment are provided and compared with other incremental learning algorithms. The results show that the DS-IILS can improve training efficiency and guarantee relatively high classification accuracy at the same time, which is consistent with the theoretical analysis.

Alkaloids from Beach Spider Lily (Hymenocallis littoralis) Induce Apoptosis of HepG-2 Cells by the Fas-signaling Pathway

Ji, Yu-Bin,Chen, Ning,Zhu, Hong-Wei,Ling, Na,Li, Wen-Lan,Song, Dong-Xue,Gao, Shi-Yong,Zhang, Wang-Cheng,Ma, Nan-Nan Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.21

Alkaloids are the most extensively featured compounds of natural anti-tumor herbs, which have attracted much attention in pharmaceutical research. In our previous studies, a mixture of major three alkaloid components (5, 6-dihydrobicolorine, 7-deoxy-trans-dihydronarciclasine, littoraline) from Hymenocallis littoralis were extracted, analyzed and designated as AHL. In this paper, AHL extracts were added to human liver hepatocellular cells HepG-2, human gastric cancer cell SGC-7901, human breast adenocarcinoma cell MCF-7 and human umbilical vein endothelial cell EVC-304, to screen one or more AHL-sensitive tumor cell. Among these cells, HepG-2 was the most sensitive to AHL treatment, a very low dose ($0.8{\mu}g/ml$) significantly inhibiting proliferation. The non-tumor cell EVC-304, however, was not apparently affected. Effect of AHL on HepG-2 cells was then explored. We found that the AHL could cause HepG-2 cycle arrest at G2/M checkpoint, induce apoptosis, and interrupt polymerization of microtubules. In addition, expression of two cell cycle-regulated proteins, CyclinB1 and CDK1, was up-regulated upon AHL treatment. Up-regulation of the Fas, Fas ligand, Caspase-8 and Caspase-3 was observed as well, which might imply roles for the Fas/FsaL signaling pathway in the AHL-induced apoptosis of HepG-2 cells.

Schedule-Dependent Effects of Kappa-Selenocarrageenan in Combination with Epirubicin on Hepatocellular Carcinoma

Ji, Yu-Bin,Ling, Na,Zhou, Xiao-Jun,Mao, Yun-Xiang,Li, Wen-Lan,Chen, Ning Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Hepatocellular carcinoma (HCC) has a relatively higher incidence in many countries of Asia. Globally, HCC has a high fatality rate and short survival. Epirubicin, a doxorubicin analogue, may be administered alone or in combination with other agents to treat primary liver cancer and metastatic diseases. However, the toxic effects of epirubicin to normal tissues and cells have been one of the major obstacles to successful cancer chemotherapy. Here, we investigated the effects of epirubicin in combination with kappa-selenocarrageenan on mice with H22 implanted tumors and HepG-2 cell proliferation, immune organ index, morphology, cell cycle and related protein expressions in vivo and in vitro with sequential drug exposure. The inhibitory rate of tumor growth in vivo was calculated. Drug sensitivity was measured by MTT assay, and the King's principle was used to evaluate the interaction of drug combination. Morphological changes were observed by fluorescent microscopy. Cell cycle changes were analyzed by flow cytometry. Expression of cyclin A, Cdc25A and Cdk2 were detected by Western blotting. In vivo results demonstrated that the inhibitory rate of EPI combined with KSC was higher than that of KSC or EPI alone, and the Q value indicated an additive effect. In addition, KSC could significantly raise the thymus and spleen indices of mice with H22 implanted tumors. In the drug sensitivity assay in vitro, exposure to KSC and EPI simultaneously was more effective than exposure sequentially in HepG-2 cells, while exposure to KSC prior to EPI was more effective than exposure to EPI prior to KSC. Q values showed an additive effect in the simultaneous group and antagonistic effects in the sequential groups. Morphological analysis showed similar results to the drug sensitivity assay. Cell cycle analysis revealed that exposure to KSC or EPI alone arrested the cells in S phase in HepG-2 cells, exposure to KSC and EPI simultaneously caused accumulation in the S phase, an effect caused by either KSC or EPI. Expression of cyclin A, Cdc25A and Cdk2 protein was down-regulated following exposure to KSC and EPI alone or in combination, exposure to KSC and EPI simultaneously resulting in the lowest values. Taken together, our findings suggest that KSC in combination with EPI might have potential as a new therapeutic regimen against HCC.

A simple method to isolate structurally and chemically intact brain vascular basement membrane for neural regeneration following traumatic brain injury

Ji Wanqing,Wu Zhiru,Wen Jiaming,Tang Hengxin,Chen Zhuopeng,Xue Bo,Tian Zhenming,Ba Yueyang,Zhang Ning,Wen Xuejun,Hou Bo 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

The brain vascular basement membrane (brain-VBM) is an important component of the brain extracellular matrix, and the three-dimensional structure of the cerebrovascular network nested with many cell-adhesive proteins may provide guidance for brain tissue regeneration. However, the potential of ability of brain-VBM to promote neural tissue regeneration has not been examined due to the technical difficulty of isolating intact brain-VBM.The present study developed a simple, effective method to isolate structurally and compositionally intact brain-VBM. Structural and component properties of the brain-VBM were characterized to confirm the technique. Seed cells were cocultured with brain-VBM in vitro to analyze biocompatibility and neurite extension. An experimental rat model of focal traumatic brain injury (TBI) induced by controlled cortical impact were conducted to further test the tissue regeneration ability of brain-VBM.Brain-VBM isolated using genipin showed significantly improved mechanical properties, was easy to handle, supported high cell viability, exhibited strong cell adhesive properties, and promoted neurite extension and outgrowth. Further testing of the isolated brain-VBM transplanted at lesion sites in an experimental rat model of focal TBI demonstrated considerable promise for reconstructing a complete blood vessel network that filled in the lesion cavity and promoting repopulation of neural progenitor cells and neurons.The technique allows isolation of intact brain-VBM as a 3D microvascular scaffold to support brain tissue regeneration following TBI and shows considerable promise for the production of naturally-derived biomaterials for neural tissue engineering.

Drying Characteristics and Models of Red Ginseng Slice Using Far-Infrared Rays

Ning Xiao Feng,Xu Ji Tong,장문기 한국농업기계학회 2021 바이오시스템공학 Vol.46 No.4

Purpose The aim of this study was to improve the drying quality and find the suitable drying model for the far-infrared drying of red ginseng slice. Methods The far-infrared drying tests on ginseng slice were conducted at two slice thickness of 2 and 4 mm and three drying temperatures of 50, 60, and 70 °C. The results were compared with the hot air drying method. The drying characteristics were analyzed based on factors such as drying rate, color changes, and elastic modulus. The Page, modified Wang and Singh, and logarithmic models were used to select the best model for far-infrared drying of red ginseng slices. Results The results revealed that increasing drying temperature and decreasing ginseng thickness caused a decrease in drying time. The color differences (ΔE) at 70 °C values were higher than 50 and 60 °C values. The highest elastic modulus (32.42 MPa) was observed at drying temperatures of 60 °C ginseng slice of 4mm. The drying quality of far-infrared dryingwas better than that of hot air drying under the same drying condition. The Page model showed the highest R2 values and lowest RMSE values than the other models. Conclusion The quality of red ginseng slice can be improved by far-infrared drying. The Page model was the best model to fit adequately most of the drying conditions for the far-infrared drying of red ginseng slice.