Parkhill

Nice Architects(나이스 아키텍츠),2KA Landscape(2케이에이 랜드스케이프) 현대건축사 2013 월간 CONCEPT Vol.- No.168

The Identification and Analysis of Selected Health Behaviors as Reported by Korean Adolescents in Seoul

Choi,Eun Jin,Nicely,Robert F.,Nicholson,Mary E.,Olsen,Larry K.,Birch,David A. 韓國保健敎育學會 1993 보건교육건강증진학회지 Vol.10 No.2

본 연구의 목적은 한국청소년들의 건강행동실태를 파악하기 위한 것이다.본 연구의 의의는 미국에서 행해지고 있는 청소년의 건강유지 및 증진과 아울러 성인병이나 조기사망에 대한 역학적인 접근방식을 한국청소년을 대상으로 하여 응용하는 데 있다.이 연구에 사용된 연구도구는 미국의 Centers for Disease Cotrol에서 청소년의 건강행동을 역학적으로 연구하기 위해 개발한 Youth Risk Behavior Survey를 한국 사회환경의 요구도에 맞게 수정한 것이다.본 연구는 한국인 청소년들의 건강행동을 조사하기 위한 타당성있는 연구도구를 개발하기 위해 4개의 단계로 구성되어 있다.두 번의 focus group discussion과 pilot test,그리고 실제 조사로 이루어져 있다. 서울지역의 중.고등학생을 대표할 표본추출은 cluster sampling을 사용하였다.Cluster는 본 연구에서 구분한 서울의 지리적인 구역들,즉 북부,남부,그리고 산업지역을 포함했고,남자학교,여자학교,남녀공학학교를 포함했다.총 38개 중.고교학생 4,747명이 조사되었다.약 52.4%의 응답작 심각하게 자살을 생각해 본 적이 있다고 답했다.그리고 23.6%가 설문조사전 30일 중에 담배를 피운 적이 있다고 응답했으며 49.7%는 설문조사전 30일 중에 술을 마신적이 있다고 답했다.약 29%가 설문조사전 30일 중에 싸움에 가담한 적이 있다고 응답했다.본 연구에서 조사된 건강관련행동(건강위험행동 포함)들은 성,연령,성적,사회경제적 위치,학교 종류,그리고 서울의 지리적인 구역에 따라 다양하게 나타났다.남학생이 여학생보다 더 건강위험행동을 나타내었다.건강위험행동은 나이가 증가함에 따라 증가했다.사회경제적 위치가 낮다고 응답한 학생들과 학급에서 성적이 하위라고 응답한 학생들의 경우 건강위험행동은 더 많이 나타났다.

Human Non-neutralizing HIV-1 Envelope Monoclonal Antibodies Limit the Number of Founder Viruses during SHIV Mucosal Infection in Rhesus Macaques

Santra, Sampa,Tomaras, Georgia D.,Warrier, Ranjit,Nicely, Nathan I.,Liao, Hua-Xin,Pollara, Justin,Liu, Pinghuang,Alam, S. Munir,Zhang, Ruijun,Cocklin, Sarah L.,Shen, Xiaoying,Duffy, Ryan,Xia, Shi-Mao Public Library of Science 2015 PLoS pathogens Vol.11 No.8

<▼1><P>HIV-1 mucosal transmission begins with virus or virus-infected cells moving through mucus across mucosal epithelium to infect CD4<SUP>+</SUP> T cells. Although broadly neutralizing antibodies (bnAbs) are the type of HIV-1 antibodies that are most likely protective, they are not induced with current vaccine candidates. In contrast, antibodies that do not neutralize primary HIV-1 strains in the TZM-bl infection assay are readily induced by current vaccine candidates and have also been implicated as secondary correlates of decreased HIV-1 risk in the RV144 vaccine efficacy trial. Here, we have studied the capacity of anti-Env monoclonal antibodies (mAbs) against either the immunodominant region of gp41 (7B2 IgG1), the first constant region of gp120 (A32 IgG1), or the third variable loop (V3) of gp120 (CH22 IgG1) to modulate <I>in vivo</I> rectal mucosal transmission of a high-dose simian-human immunodeficiency virus (SHIV-BaL) in rhesus macaques. 7B2 IgG1 or A32 IgG1, each containing mutations to enhance Fc function, was administered passively to rhesus macaques but afforded no protection against productive clinical infection while the positive control antibody CH22 IgG1 prevented infection in 4 of 6 animals. Enumeration of transmitted/founder (T/F) viruses revealed that passive infusion of each of the three antibodies significantly reduced the number of T/F genomes. Thus, some antibodies that bind HIV-1 Env but fail to neutralize virus in traditional neutralization assays may limit the number of T/F viruses involved in transmission without leading to enhancement of viral infection. For one of these mAbs, gp41 mAb 7B2, we provide the first co-crystal structure in complex with a common cyclical loop motif demonstrated to be critical for infection by other retroviruses.</P></▼1><▼2><P><B>Author Summary</B></P><P>Antibodies specifically recognize antigenic sites on pathogens and can mediate multiple antiviral functions through engagement of effector cells via their Fc region. Current HIV-1 vaccine candidates induce polyclonal antibody responses with multiple antiviral functions, but do not induce broadly neutralizing antibodies. An improved understanding of whether certain types of non-neutralizing HIV-1 specific antibodies can individually protect against HIV-1 infection may facilitate vaccine development. Here, we test whether non-neutralizing antibodies with multiple antiviral functions mediated through FcR engagement and recognition of virus particles or virus-infected cells can limit infection, despite lacking classical virus neutralization activity. In a passive antibody infusion-rhesus macaque challenge model, we tested the ability of non-neutralizing monoclonal antibodies to limit virus acquisition. We demonstrate that two different types of non-neutralizing antibodies, one that recognizes both virus particles and infected cells (7B2) and another that recognizes only infected cells (A32) were capable of decreasing the number of transmitted founder viruses. Further, we provide the structure of 7B2 in complex with the gp41 cyclical loop motif, a motif critical for entry. These findings provide insights into the role that antibodies with antiviral properties, including virion capture and FcR mediated effector function, may play in protecting against HIV-1 acquisition.</P></▼2>

Quest for Missing Proteins: Update 2015 on Chromosome-Centric Human Proteome Project

Horvatovich, Pé,ter,Lundberg, Emma K.,Chen, Yu-Ju,Sung, Ting-Yi,He, Fuchu,Nice, Edouard C.,Goode, Robert J.,Yu, Simon,Ranganathan, Shoba,Baker, Mark S.,Domont, Gilberto B.,Velasquez, Erika,Li, D American Chemical Society 2015 Journal of Proteome Research Vol.14 No.9

<P>This paper summarizes the recent activities of the Chromosome-Centric Human Proteome Project (C-HPP) consortium, which develops new technologies to identify yet-to-be annotated proteins (termed “missing proteins”) in biological samples that lack sufficient experimental evidence at the protein level for confident protein identification. The C-HPP also aims to identify new protein forms that may be caused by genetic variability, post-translational modifications, and alternative splicing. Proteogenomic data integration forms the basis of the C-HPP’s activities; therefore, we have summarized some of the key approaches and their roles in the project. We present new analytical technologies that improve the chemical space and lower detection limits coupled to bioinformatics tools and some publicly available resources that can be used to improve data analysis or support the development of analytical assays. Most of this paper’s content has been compiled from posters, slides, and discussions presented in the series of C-HPP workshops held during 2014. All data (posters, presentations) used are available at the C-HPP Wiki (<uri xlink:href='http://c-hpp.webhosting.rug.nl/' xlink:type='simple'>http://c-hpp.webhosting.rug.nl/</uri>) and in the Supporting Information.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jprobs/2015/jprobs.2015.14.issue-9/pr5013009/production/images/medium/pr-2014-013009_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/pr5013009'>ACS Electronic Supporting Info</A></P>

Laser-activatable Oxygen Self-supplying Nanoplatform for Efficiently Overcoming Colorectal Cancer Resistance by Enhanced Ferroptosis and Alleviated Hypoxic Microenvironment

Hao Jiang,Hailong Tian,Zhihan Wang,Bowen Li,Rui Chen,Kangjia Luo,Shuaijun Lu,Edouard C. Nice,Wei Zhang,Canhua Huang,Yuping Zhou,Shaojiang Zheng,Feng Gao 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

Background Colorectal cancer (CRC) is the second most deadly cancer worldwide, with chemo-resistance remaining a major obstacle in CRC treatment. Notably, the imbalance of redox homeostasis-mediated ferroptosis and the modulation of hypoxic tumor microenvironment are regarded as new entry points for overcoming the chemo-resistance of CRC. Methods Inspired by this, we rationally designed a light-activatable oxygen self-supplying chemo-photothermal nanoplatform by co-assembling cisplatin (CDDP) and linoleic acid (LA)-tailored IR820 via enhanced ferroptosis against colorectal cancer chemo-resistance. In this nanoplatform, CDDP can produce hydrogen peroxide in CRC cells through a series of enzymatic reactions and subsequently release oxygen under laser-triggered photothermal to alleviate hypoxia. Additionally, the introduced LA can add exogenous unsaturated fatty acids into CRC cells, triggering ferroptosis via oxidative stress-related peroxidized lipid accumulation. Meanwhile, photothermal can efficiently boost the rate of enzymatic response and local blood flow, hence increasing the oxygen supply and oxidizing LA for enhanced ferroptosis. Results This nanoplatform exhibited excellent anti-tumor efficacy in chemo-resistant cell lines and showed potent inhibitory capability in nude mice xenograft models. Conclusions Taken together, this nanoplatform provides a promising paradigm via enhanced ferroptosis and alleviated hypoxia tumor microenvironment against CRC chemo-resistance.

The Identification and Analysis of Selected Health Behaviors as Reported by Korean Adolescents in Seoul

Eun Jin Choi(최은진),Larry K. Olsen,Mary E. Nicholson,David A. Birch,Robert F. Nicely 한국보건교육건강증진학회 1993 보건교육건강증진학회지 Vol.10 No.2