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      • KCI등재

        A novel edible coating of beeswax impregnated with karonda polyphenol rich extract maintains the chemical and bioactive potential of fresh ber fruit during storage at low temperature

        Kaur Karandeep,Gupta Navjot,Mahajan Monika,Jawandha Sukhjit Kaur,Kaur Nirmaljit 한국원예학회 2023 Horticulture, Environment, and Biotechnology Vol.64 No.6

        Ber fruit is highly nutritious and rich in bioactive compounds known to have several health benefi ts. However, the signifi cant loss of such compounds during long-term storage is a concern. Retaining its quality during post-harvest by natural preservatives could be a novel approach towards sustainability. In the present study, a novel composite edible coating of beeswax and polyphenols was developed. Beeswax, a lipid-based material with anti-transpiration properties, was derived from honeycomb and polyphenols with huge antioxidant and antimicrobial properties were extracted from underutilized fruit, karonda. The purpose of the present work is to evaluate the eff ect of this composite coating on the storage life of ber fruits. For this, beeswax (2%) alone or impregnated with the polyphenols at diff erent concentrations 0.5% (300 mg/L), 1% (600 mg/L) and 1.5% (750 mg/L) was applied on the fruit's surface. The eff ect of this composite edible coating on the physicochemical properties, bioactive compounds and antioxidant enzymes activities of ber fruits was studied for 28 days during cold storage. The results revealed that the combination of beeswax coating with karonda polyphenols markedly reduced spoilage and maintained the fi rmness of ber fruit during storage. The coating has resulted in higher retention of ascorbic acid, fl avonoids, phenolics and antioxidant activity during storage. The treated fruits showed delayed ripening by slowing down the synthesis of carotenoids as compared to the control fruit. Further, the coating resulted in the lower activities of cell wall degrading enzymes like pectin methyl esterase and cellulase enzymes in the stored ber fruits. Moreover, the concentrations of karonda polyphenols (KPP) have worked in a dose-dependent manner in the coating system, as beeswax with 1.5% KPP was found to be more eff ective in maintaining the quality and nutritional potential of ber fruit during storage.

      • KCI등재

        Marine Microbe as Nano-factories for Copper Biomineralization

        Harneet Kaur,Kunzes Dolma,Navjot Kaur,Ankit Malhotra,Navinder Kumar,Pooja Dixit,Deepak Sharma,S. Mayilraj,Anirban Roy Choudhury 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1

        The present research work highlighted thepossibility of exploiting marine microbes for the biosynthesisof copper nanoparticles and further investigated thermodynamicchanges during the biomineralization of copper ina microbial system by isothermal titration calorimetry. Abacterial strain (M-7) isolated from Kanyakumari coast,India was found capable of synthesizing these nanoparticles. Based on phylogenetic relationship, the strain M-7 wasidentified as Kocuria flava. The nanoparticles producedwere characterized using UV-vis spectrophotometry, Transmissionelectron microscopy (TEM) and Dynamic lightscattering (DLS). Data revealed the formation of sphericaland quasi-spherical shaped nanoparticles with an averageparticle size ranging between 5 and 30 nm. Additionally, astudy on the effect of different media components indicatedthat media containing higher amount of casein enzymichydrolysate and yeast extract supported the formationof stable colloidal suspension of nanoparticles. Finally,isothermal titration calorimetry (ITC) was carried out tounderstand the change in heat energy during the formationof nanoparticles in different media. Combinatorial observationsof all these studies may open up new strategies todevelop tailor made copper nanoparticles via green route.

      • KCI등재

        Characterization of Pyrenophora tritici-repentis (Tan Spot of Wheat) Races in Baltic States and Romania

        Sidrat Abdullah,Sunish Kumar Sehgal,Shaukat Ali,Zilvinas Liatukas,Mariana Ittu,Navjot Kaur 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.2

        Tan spot, caused by the fungus Pyrenophora triticirepentis,is economically important foliar disease inLatvia, Lithuania, and Romania; however, race structurefrom Baltic States and Romania is not known. Inthis study, we performed genotypic and phenotypicrace characterization of a large collection of P. triticirepentisisolates from these countries to determinerace structure and utilize this information for betterdisease management and breeding wheat for tanspot resistance. We characterized 231 single sporeisolates from Latvia (n = 15), Lithuania (n = 107), andRomania (n = 109) for Ptr ToxA and Ptr ToxB genesusing two genes specific primers. A subset (139) of231 isolates were further characterized for their racestructure by inoculating them individually on tan spotwheat differentials set. Majority (83%) of the 231 isolatesamplified Ptr ToxA gene suggesting prevalenceof race 1 and 2. Further, phenotypic characterizationof 139 isolates also showed wide prevalence of races 1(68%), 2 (8%), 3 (11%), and 4 (5%) were also identifiedfrom Baltic States as well as Romania. Eighteenof the isolates (13%) did not seem to be of any of theeight known races as they lacked Ptr ToxA gene butthey behaved like either race 1 or race 2, suggestingpossibility of novel toxins in these isolates as theirvirulence tools.

      • SCIEKCI등재

        Characterization of Pyrenophora tritici-repentis (Tan Spot of Wheat) Races in Baltic States and Romania

        Abdullah, Sidrat,Sehgal, Sunish Kumar,Ali, Shaukat,Liatukas, Zilvinas,Ittu, Mariana,Kaur, Navjot The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.2

        Tan spot, caused by the fungus Pyrenophora tritici-repentis, is economically important foliar disease in Latvia, Lithuania, and Romania; however, race structure from Baltic States and Romania is not known. In this study, we performed genotypic and phenotypic race characterization of a large collection of P. tritici-repentis isolates from these countries to determine race structure and utilize this information for better disease management and breeding wheat for tan spot resistance. We characterized 231 single spore isolates from Latvia (n = 15), Lithuania (n = 107), and Romania (n = 109) for Ptr ToxA and Ptr ToxB genes using two genes specific primers. A subset (139) of 231 isolates were further characterized for their race structure by inoculating them individually on tan spot wheat differentials set. Majority (83%) of the 231 isolates amplified Ptr ToxA gene suggesting prevalence of race 1 and 2. Further, phenotypic characterization of 139 isolates also showed wide prevalence of races 1 (68%), 2 (8%), 3 (11%), and 4 (5%) were also identified from Baltic States as well as Romania. Eighteen of the isolates (13%) did not seem to be of any of the eight known races as they lacked Ptr ToxA gene but they behaved like either race 1 or race 2, suggesting possibility of novel toxins in these isolates as their virulence tools.

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