Self-Transmissible IncP R995 Plasmids with Alternative Markers and Utility for Flp/FRT Cloning Strategies

( Clayton P. Santiago ),( Laura N. Quick ),( James W. Wilson ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.11

The IncP plasmid R995 has been a useful selftransmissible, broad-host-range vector for a number of applications including the recombinase/conjugation-based cloning of large genomic DNA segments. However, R995 derivatives (or related plasmids) expressing a wide range of different resistance markers and Flp recombinase target sites do not exist in the literature. In addition, documented strategies for applying such plasmids in cloning applications that take advantage of conjugation for the convenient isolation and recovery of constructs are extremely limited. Here, we report a new series of R995 plasmids with alternative markers to increase options for applications in backgrounds already expressing resistance to a particular antibiotic(s). These R995 plasmids have been engineered to contain FRT sites that can be used for recombinase-based cloning. We demonstrate the utility of this approach by cloning 20 kb regions from the Salmonella Typhimurium and Escherichia coli genomes and by cloning DNA from an exogenous plasmid source. To our knowledge, this represents the first systematic engineering of an intact, self-transmissible IncP plasmid with a series of alternative antibiotic markers and FRT sites.

Penile inversion through a penoscrotal incision for the treatment of penile urethral strictures

Jonathan N Warner,James M Tracey,Ali A Zhumkhawala,Kevin G. Chan,Clayton S. Lau 대한비뇨의학회 2016 Investigative and Clinical Urology Vol.57 No.2

Purpose: This article describes a novel technique for the repair of penile urethral strictures and establishes the safety, feasibility, and efficacy of this innovative surgical approach. Materials and Methods: Patients with urethral strictures underwent a one-sided anterior dorsal oral mucosal graft urethroplasty through a penoscrotal inversion technique. The clinical outcome was considered a failure when any instrumentation was needed postoperatively, including dilatation. Results: Five patients underwent the novel procedure. The patients' mean age was 58 years. The cause of stricture was instrumentation in 2 cases (40%), lichen sclerosis in 1 case (20%), and failed hypospadias repair in 2 cases (40%). The mean stricture length was 3 cm. The overall mean (range) follow-up was 6 months (range, 3–9 months). Of the 5 patients, 4 (80%) had a successful outcome and 1 (20%) had a failed outcome. The failure was successfully treated by use of a meatotomy. Conclusions: The penile inversion technique through a penoscrotal incision is a viable option for the management of penile urethral strictures with several advantages to other techniques: namely, no penile skin incision, a single-stage operation, and supine positioning.

Growth, structure and physical properties of single crystals of pure and Pb-doped Bi-based high Tc superconductors

E. Giannini,R. Gladyshevskii,N. Clayton,N. Musolino,V. Garnier,A. Piriou,R. Flu¨kiger 한국물리학회 2008 Current Applied Physics Vol.8 No.2

Single crystals of (Bi1xPbx)2Sr2Ca2Cu3O10+d (x = 0 and 0.16) (sizes up to 3 · 2 · 0.1 mm3) have been grown by means of a newly developed ‘‘vapour-assisted travelling solvent floating zone’’ technique (VA-TSFZ). Post-annealing under high pressure of O2 (up to 10 MPa at T = 500 C) was applied to enhance Tc (up to 111 K) and improve the homogeneity of the crystals (DTc 6 1 K). The structure of both Pb-free and Pb-doped Bi-2223 was refined for the first time from single crystal X-ray diffraction (XRD) data. The unit cell of the average structure is pseudo-tetragonal with a = 5.4210(7), b = 5.4133(6) and c = 37.010(7)A ˚ , and a = 5.395(1), b = 5.413(1) and c = 37.042(11)A ˚ , for the Pb-free and the Pb-doped phase, respectively. An incommensurate modulation in the direction of one of the short cell vectors has been defined (q 0.21 a*), however, the structure can be conveniently described in a supercell with a fivefold volume (a = 27.105(4) A ˚ ). With respect to the ‘‘non-modulated’’ structure, one additional oxygen atom for ten initial O was found to be inserted into the BiO layers. The superconducting anisotropy of Bi-2223 was found to be 50, from measurements of the lower critical field. The anisotropy of Bi-2223 is significantly reduced compared to that of Bi-2212, and this accounts for the enhanced irreversibility fields in Bi-2223. Furthermore, Bi-2223 has a higher critical current density, and a reduced magnetic relaxation rate compared to Bi-2212, which are both signatures of more effective pinning in Bi-2223 due to its reduced anisotropy. Single crystals of (Bi1xPbx)2Sr2Ca2Cu3O10+d (x = 0 and 0.16) (sizes up to 3 · 2 · 0.1 mm3) have been grown by means of a newly developed ‘‘vapour-assisted travelling solvent floating zone’’ technique (VA-TSFZ). Post-annealing under high pressure of O2 (up to 10 MPa at T = 500 C) was applied to enhance Tc (up to 111 K) and improve the homogeneity of the crystals (DTc 6 1 K). The structure of both Pb-free and Pb-doped Bi-2223 was refined for the first time from single crystal X-ray diffraction (XRD) data. The unit cell of the average structure is pseudo-tetragonal with a = 5.4210(7), b = 5.4133(6) and c = 37.010(7)A ˚ , and a = 5.395(1), b = 5.413(1) and c = 37.042(11)A ˚ , for the Pb-free and the Pb-doped phase, respectively. An incommensurate modulation in the direction of one of the short cell vectors has been defined (q 0.21 a*), however, the structure can be conveniently described in a supercell with a fivefold volume (a = 27.105(4) A ˚ ). With respect to the ‘‘non-modulated’’ structure, one additional oxygen atom for ten initial O was found to be inserted into the BiO layers. The superconducting anisotropy of Bi-2223 was found to be 50, from measurements of the lower critical field. The anisotropy of Bi-2223 is significantly reduced compared to that of Bi-2212, and this accounts for the enhanced irreversibility fields in Bi-2223. Furthermore, Bi-2223 has a higher critical current density, and a reduced magnetic relaxation rate compared to Bi-2212, which are both signatures of more effective pinning in Bi-2223 due to its reduced anisotropy.

TFOS DEWS II Introduction

Nelson, J. Daniel,Craig, Jennifer P.,Akpek, Esen K.,Azar, Dimitri T.,Belmonte, Carlos,Bron, Anthony J.,Clayton, Janine A.,Dogru, Murat,Dua, Harminder S.,Foulks, Gary N.,Gomes, José,A.P.,Hammitt, Elsevier 2017 The ocular surface Vol.15 No.3

THE BRIGHTEST YOUNG STAR CLUSTERS IN NGC 5253

Calzetti, D.,Johnson, K. E.,Adamo, A.,Gallagher III, J. S.,Andrews, J. E.,Smith, L. J.,Clayton, G. C.,Lee, J. C.,Sabbi, E.,Ubeda, L.,Kim, H.,Ryon, J. E.,Thilker, D.,Bright, S. N.,Zackrisson, E.,Kennic IOP Publishing 2015 The Astrophysical journal Vol.811 No.2

<P>The nearby dwarf starburst galaxy NGC 5253 hosts a number of young, massive star clusters, the two youngest of which are centrally concentrated and surrounded by thermal radio emission (the 'radio nebula'). To investigate the role of these clusters in the starburst energetics, we combine new and archival Hubble Space Telescope images of NGC 5253 with wavelength coverage from 1500 angstrom 1.9 mu m in 13 filters. These include H alpha, P beta, and P alpha, and the imaging from the Hubble Treasury Program LEGUS (Legacy Extragalactic UV Survey). The extraordinarily well-sampled spectral energy distributions enable modeling with unprecedented accuracy the ages, masses, and extinctions of the nine optically brightest clusters (M-V < -8.8) and the two young radio nebula clusters. The clusters have ages similar to 1-15 Myr and masses similar to 1 x 10(4)-2.5 x 10(5) M-circle dot. The clusters' spatial location and ages indicate that star formation has become more concentrated toward the radio nebula over the last similar to 15 Myr. The most massive cluster is in the radio nebula; with a mass similar to 2.5 x 10(5) M-circle dot and an age similar to 1 Myr, it is 2-4 times less massive and younger than previously estimated. It is within a dust cloud with AV similar to 50 mag, and shows a clear near-IR excess, likely from hot dust. The second radio nebula cluster is also similar to 1 Myr old, confirming the extreme youth of the starburst region. These two clusters account for about half of the ionizing photon rate in the radio nebula, and will eventually supply about 2/3 of the mechanical energy in present-day shocks. Additional sources are required to supply the remaining ionizing radiation, and may include very massive stars.</P>

LEGACY EXTRAGALACTIC UV SURVEY (LEGUS) WITH THE<i>HUBBLE SPACE TELESCOPE</i>. I. SURVEY DESCRIPTION

Calzetti, D.,Lee, J. C.,Sabbi, E.,Adamo, A.,Smith, L. J.,Andrews, J. E.,Ubeda, L.,Bright, S. N.,Thilker, D.,Aloisi, A.,Brown, T. M.,Chandar, R.,Christian, C.,Cignoni, M.,Clayton, G. C.,Silva, R. da,Mi IOP Publishing 2015 The Astronomical journal Vol.149 No.2