Radiotherapy-associated Furin Expression and Tumor Invasiveness in Recurrent Laryngeal Cancer

LEE, MYUNGJIN,RYU, CHANG HWAN,CHANG, HYO WON,KIM, GUI CHUL,KIM, SEONG WHO,KIM, SANG YOON Potamitis Press 2016 Anticancer research Vol.36 No.10

<P>Background/Aim: Recurrent laryngeal cancer often shows an aggressive phenotype after radiotherapy and does not respond to conventional therapeutic strategies. In this study, we investigated the contribution of furin to cellular invasiveness in radio-resistant laryngeal cancer. Materials and Methods: Using previously established AMC-HN-3 and AMC-HN-8 cell lines from laryngeal carcinoma patients, recurrent laryngeal cancer models were generated by cumulative irradiation (AMC-HN-3-70Gy and AMC-HN-8-70Gy). Immunocytochemistry and western blotting were used to determine the epithelial-mesenchymal transition (EMT). Invasion capacity was assessed using an in vitro invasion assay. Zymography was used to assess metalloproteinase-2 (MMP-2) activity. Tumor xenografts were developed to compare growth rate and furin expression in vivo. Furin expression in 35 patients (45 samples) with salvage total laryngectomy after radiation-based treatment was assessed by laryngeal cancer tissue microarray. Results: Both AMC-HN-3-70Gy and AMC-HN-8-70Gy cell lines underwent EMT following radiation. However, AMC-HN-3-70Gy cells showed increased cellular invasiveness, whereas AMC-HN-8-70Gy cells showed no difference. AMC-HN-3-70Gy cells also exhibited elevated furin expression with up-regulated expression of the active form of membrane type 1-matrix metalloproteinase (MT1MMP)/MMP-2, whereas AMC-HN-8-70Gy cells did not show significant changes. After administration of a furin inhibitor (chloromethyl ketone (CMK)), AMC-HN-3-70Gy cells showed a significant decrease in MT1-MMP/MMP-2 expression and cellular invasiveness. Nine of 22 samples (40.9%) from salvage total laryngectomy and one of 13 pre-radiation samples (7.7%) had high furin expression. Post-radiation, furin expression increased in seven of 10 patients whose pre- and post-radiation samples were available; all-cancer mortality (three patients) was observed in this group. Conclusion: Together with EMT, furin activity may serve as an indicator of an aggressive cancer phenotype, suggesting that furin is a potentially useful target for recurrent laryngeal cancer.</P>

Estimation of optimal runoff hydrograph using radar rainfall ensemble and blending technique of rainfall-runoff models

Lee, Myungjin,Kang, Narae,Kim, Jongsung,Kim, Hung Soo 한국수자원학회 2018 한국수자원학회논문집 Vol.51 No.3

최근 기후변화로 인한 국지성 호우 및 태풍 피해가 자주 발생하고 있다. 이와 같은 피해를 저감하기 위해서는 정확한 강우의 예측과 홍수량 산정이 필요하다. 그러나 지점 및 레이더 강우 시 ․ 공간적 오차를 포함하고 있고, 유출 모형에 의한 유출수문곡선 역시 보정을 실시하더라도 관측유량과 오차를 가지고 있어 불확실성이 존재한다. 따라서 본 연구에서는 확률론적 강우 앙상블을 생성하여 강우의 불확실성을 확인하였다. 또한 유출 결과를 통해 수문 모형의 불확실성을 확인하였고, 블랜딩 기법을 이용하여 하나의 통합된 유출 수문곡선을 제시하였다. 생성된 강우앙상블은 강우강도 및 지형적인 영향으로 레이더가 과소 관측이 될 때, 강우 앙상블의 불확실성이 큰 것을 확인하였고, 블랜딩 기법을 적용하여 산정된 최적 유출 수문곡선은 유출모형의 불확실성을 크게 줄이는 것으로 나타났다. 본 연구 결과를 활용한다면, 정확한 홍수량 산정 및 예측을 통해 집중호우로 인한 피해를 줄일 수 있을 것으로 판단된다. Recently, the flood damage by the localized heavy rainfall and typhoon have been frequently occurred due to the climate change. Accurate rainfall forecasting and flood runoff estimates are needed to reduce such damages. However, the uncertainties are involved in guage rainfall, radar rainfall, and the estimated runoff hydrograph from rainfall-runoff models. Therefore, the purpose of this study is to identify the uncertainty of rainfall by generating a probabilistic radar rainfall ensemble and confirm the uncertainties of hydrological models through the analysis of the simulated runoffs from the models. The blending technique is used to estimate a single integrated or an optimal runoff hydrograph by the simulated runoffs from multi rainfall-runoff models. The radar ensemble is underestimated due to the influence of rainfall intensity and topography and the uncertainty of the rainfall ensemble is large. From the study, it will be helpful to estimate and predict the accurate runoff to prepare for the disaster caused by heavy rainfall.

A Low-Foaming and Biodegradable Surfactant as a Soil-Flushing Agent for Diesel-Contaminated Soil

Lee, Young-Chul,Choi, Moon-Hee,Han, Jong-In,Lim, Yoo Lan,Lee, Myungjin Taylor Francis 2013 Separation science and technology Vol.48 No.12

Photo-oxidation activities on Pd-doped TiO₂ nanoparticles: critical PdO formation effect

Lee, Hangil,Shin, Minjeong,Lee, Myungjin,Hwang, Yun Jeong Elsevier 2015 Applied Catalysis B Vol.165 No.-

<P><B>Abstract</B></P> <P>The catalytic activities of three distinct Pd-doped TiO<SUB>2</SUB> (PdTiO<SUB>2</SUB>) nanoparticle samples, post-annealed (at 700, 800, and 900°C) after fabrication on silicon substrates, were analyzed with respect to the photo-oxidation of aniline and 2-thiophenecarboxaldehyde. Through a combination of Raman spectroscopy and high-resolution photoemission spectroscopy (HRPES), we demonstrated that the photocatalytic oxidation occurred only when the PdTiO<SUB>2</SUB> nanoparticles were annealed at temperatures above 800°C, that is, when PdO and Ti<SUP>3+</SUP> had partially formed on the PdTiO<SUB>2</SUB> nanoparticles, which indicates that the presence of PdO and Ti<SUP>3+</SUP> is critical for the photocatalytic oxidation. Additionally, we determined the decrease in the band gaps to visible region with PdTiO<SUB>2</SUB> nanoparticles annealed above 800°C from their valence-band spectra, which resulted in photocatalytic activities even under visible light, <I>λ</I> =540nm.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced photocatalytic oxidation activity of PdTiO<SUB>2</SUB> nanoparticles with existence of PdO. </LI> <LI> PdO formation in PdTiO<SUB>2</SUB> nanoparticles by thermal annealing above 800°C. </LI> <LI> Capability for photo-oxidation of aniline and 2-thiophenecarboxaldehyde under visible light. </LI> <LI> Decrease of PdTiO<SUB>2</SUB> band gap to visible region (1.92eV) by thermal annealing at 900°C. </LI> </UL> </P>

Non-Contact Measurement of Current Distribution in Parallel Conductors by Using Hall Sensors

Myungjin Park,Sangbeom Byun,Wooseok Kim,Jikwang Lee,Kyeongdal Choi,Haigun Lee IEEE 2008 IEEE transactions on applied superconductivity Vol.18 No.2

<P>A parallel HTS conductor to flow large current is essential for applications of HTS power device. Non-uniform current distribution in the parallel conductor is caused mainly due to the difference of inductance. There are a contact method which measures current directly and a non-contact method which measure indirectly. But current distribution of each conductor in parallel conductors can not be measured with conventional method. In this paper, we researched a measurement method to identify current distribution of the parallel conductor indirectly with Hall sensors and compared with the analysis results by 2-D FEM.</P>

Antiangiogenic Activity of Orally Absorbable Heparin Derivative in Different Types of Cancer Cells

Lee, Dong Yun,Lee, Sung Won,Kim, Sang Kyoon,Lee, Myungjin,Chang, Hyo Won,Moon, Hyun Tae,Byun, Youngro,Kim, Sang Yoon Springer-Verlag 2009 Pharmaceutical research Vol.26 No.12

Effect of Aluminum on Thermally Induced ε-Martensite for Fe-Mn-C TWIP Steels

Myungjin Lee,박철호,Eun-Joon Chun,Juseung Lee,강남현 대한용접접합학회 2019 대한용접·접합학회지 Vol.37 No.1

Cold-rolling of hot-rolled plate of Fe-18.7Mn-0.61C-1.4Al (Al-TWIP) steel was identified with an austenite single phase through the entire thickness, and that of Fe-17.8Mn-0.57C (TWIP) steel was composed of ε-martensite and austenite phases from the surface to a thickness of ~45 ㎛. Electron back-scattered diffraction (EBSD), glow discharge spectrometry (GDS), and transmission electron microscopy (TEM) were conducted to investigate the ε-martensite transformation of TWIP steel. In TWIP steel, thermally induced ε-martensite transformation was produced since the surface austenite stability decreased due to surface decarburization. The thickness of ε-martensite (~45 ㎛) was coincident with the depth at which 0.45 wt% C of TWIP steel was achieved. However, the TWIP steel with added Al increased the austenite stability of the surface layer and suppressed ε-martensite transformation.

Polyproline-type helical-structured low-molecular weight heparin (LMWH)-taurocholate conjugate as a new angiogenesis inhibitor

Lee, Esak,Kim, Yoo-Shin,Bae, Sang Mun,Kim, Sang Kyoon,Jin, Shunji,Chung, Seung Woo,Lee, Myungjin,Moon, Hyun Tae,Jeon, Ok-Cheol,Park, Rang Woon,Kim, In San,Byun, Youngro,Kim, Sang Yoon Wiley Subscription Services, Inc., A Wiley Company 2009 International journal of cancer: Journal internati Vol.124 No.12

<P>Although heparin can regulate angiogenesis, tumor growth and metastasis, its clinical application, as well as that of low-molecular heparin (LMWH), for treating cancer are limited because of heparin's anticoagulant activity and risk of hemorrhages. LMWH-taurocholate conjugates (LHT7), which have low anticoagulant activity, were synthesized. The structural property of LHT was evaluated by circular dichroism and the binding affinity of LHT7 to vascular endothelial growth factor 165 (VEGF<SUB>165</SUB>) was measured by isothermal titration calorimetry. The inhibitory effect of LHT7 on VEGF-mediated KDR (VEGF-receptor 2) phosphorylation in Human umbilical vein endothelial cells was evaluated. The VEGF<SUB>165</SUB> dependent Matrigel plug assay was performed to verify the antiangiogenic potential of LHT7 on a VEGF<SUB>165</SUB> inhibitor. Finally, tumor growth inhibition effects of LHT7 on SCC7 and the survival rate of animal models were investigated. Moreover, MDA-MB231 xenograft mouse model was additionally used to confirm the therapeutic effect of LHT7 on human breast cancer cell line. As a result, LHT7 which has 12.7% of anticoagulant activity of the original LMWH showed a peculiar polyproline-type helical structure. LHT7 binds to VEGF strongly and inhibits VEGF dependent KDR phosphorylation. The results of Matrigel plug assay proved LHT7 as a strong antiangiogenic agent inhibiting VEGF<SUB>165</SUB>. Remarkably, LHT7 showed a significant tumor growth inhibition potential on SCC7 with an increased survival rate. LHT7 also delayed tumor growth in MDA-MB231 human breast cancer cell lines. © 2008 UICC</P>

Inter-row Adsorption Configuration and Stability of Threonine Adsorbed on the Ge(100) Surfaces

Lee, Myungjin,Park, Youngchan,Jeong, Hyuk,Lee, Hangil Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

The adsorption structures of threonine on the Ge(100) surface were investigated using core-level photoemission spectroscopy (CLPES) in conjunction with density functional theory (DFT) calculations. CLPES measurements were performed to identify the experimentally preferred adsorption structure. The preferred structure indicated the relative reactivities of the carboxyl and hydroxymethyl groups as electron donors to the Ge(100) surface during adsorption. The core-level C 1s, N 1s, and O 1s CLPES spectra indicated that the carboxyl oxygen competed more strongly with the hydroxymethyl oxygen during the adsorption reaction. Three among six possible adsorption structures were identified as energetically favorable using DFT calculation methods that considered the inter- and intra-bonding configurations upon adsorption onto the Ge(100) surface. These structures were O-H dissociated N dative inter bonding, O-H dissociated N dative intra bonding, O-H dissociation bonding. One of the adsorption structures: O-H dissociated N dative inter bonding was predicted to be stable in light of the transition state energies. We thus confirmed that the most favorable adsorption structure is the O-H dissociated N dative-inter bonding structure using CLPES and DFT calculation.

Comparison of Magnetic Field and AC Losses in Solenoid Coil and Pancake Coil with HTS tape

Myungjin Park,Kwangyoun Lee,Jungwook Sim,Gueesoo Cha,Jikwang Lee 한국초전도저온학회 (구 한국초전도저온공학회) 2003 한국초전도저온공학회논문지 Vol.5 No.1