배양된 망막색소 상피세포의 탐식기능

고명규,최준규,김동섭 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.2

This study was performed to observe the phagocytosis of latex bead in cultured human retinal pigment epithelium (RPE). RPE cells were isolated from retina of enucleated human eye and 5 to 7㎛ diameter latex beads were added to culture media of human RPE cells at 50th day of culture. Phagocytic processes were observed serially by phase-contrast microscopy and ight microscopy up to 58 days. After a latent period of about 16hours, latex particles were rapidly phagocytized by cultured human RPE cells. These result may indicate that PRE cells can be cultured up to 58 days without subculture and phamocytic property of RPE remains an that time.

노인에 있어서 시력 상실의 흔한 원인

申正澈,高明圭 대한의사협회 2000 대한의사협회지 Vol.43 No.4

자가방수액이 가토 결막조직에 미치는 영향

신경학,고명규,최준규 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.2

It has been well known that the failure of the trabeculectomy is associated with the migration and proliferation of the fibroblast at the fistula site, which may be influenced by the aqueous humor. It is not clear whether the aqueous humor nas growth factor or inhibitory factor. This experiment was designed to elucidate the effect of the aqueous humor on the conjunctiva in rabbit. The aqueous humor was injected into the subconjunctival area in the rabbit, twice weekly upto for 1 minth. In addition, the autologous conjunctival tissue was implanted into the anterior chamber of the fellow eye, which was observed for three months. The conjuntiva, which was treated by the aqueous humor in situ and in anterior chamber, was examined with electron microscopy. The conjunctiva, treated with autologous aqueous humor, showed the infiltration of the inflammatory cells and fivrin in the substantia propria. These findings may be affected by the disruption of the blood-aqueous barrier or chemoattractant activity of the aqueous humor. The conjunctiva, implanted into the anterior chamber, disappeared with fragmentation or was shrinked in appearance. The conjunctival epithelial cells showed the degeneration with widened intercellular space, slightly protruded villous projection into intercellular space and separation of desmosome between adjacent cells. The substantia propria was well preserved with both the endothelial and pericyte cell uninterruptedly lined with basement membrane. The widened intercellular space and slightly projected villous projection may be influenced by the growth activity in the aqueous humor. However, the inhibitory activity in the aqueous humor may contributetothe degeneration in teh superficial epithelium and no further growth of the conjuntival tissue. These fundings may be summed up by the growth activity of the plasmoid aqueous by disruption of blood-aqueous barrier following surgical manipulation and by inhibitory activity in stabilized aqueous humor. The influence of aqueous homor on the cytologic or histologic pattern will depend on the biochemical components of the aqueous humor.

선천 섬유화 증후군 1예

김동섭,신정철,고명규,최준규,장세진 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

The congential fibrosis syndorme is rare congenital disorder which is characterized by blepharoptosis, varying degrees of limitation of eye movement, especially in vertical gaze, and abnormal head position. These clinical findings result from the fibrous replacement of normal contractile extraocular muscle tissue. The authors report one sporadic case of congential fibrosis syndrome. The 14 year-old-girl showed bilateral ptosis, fixed ocular misalignment in downward gaze, limited ductions and versions, and characteristic chin up position. The surgical management attempted to achieve functional readjustment of the ocular alignment and lid position and to correct cosmetically unacceptable abnormal head position. Result of histopathologic examination obtained at surgery shows replacement of the extraocular muscles, conjuctiva and Tenon's capsule by collagen and dense fibrous tissue. The surgical outcome is considered satisfactory when compared with preoperative status although the limitation of vertical eye movement remains unchanged after surgery.

Sodium Hyaluronate가 가토 결막상피에 미치는 형태학적 연구

유석찬,고명규,최준규 한양대학교 의과대학 1991 한양의대 학술지 Vol.11 No.1

This study was carried out whether sodium hyaluronate may affect the mucous layer of the tear film and surface layer of the conjunctival epithelium by histochemical electron microscopy. Sodium hyaluronate was injected into the subconjunctival tissue of five rabbits twice a week for 10 weeks. The excised specimen was treated with ruthenium red to enhance the presence of glycosaminoglycans of the surface layer of the conjunctiva. From the histochemical electron microscopic point of view, sodium hyaluronate did not improve the content of glycosaminoglycans density in the tear film over the surface of the conjunctival dpithelium. In addition, there was no evidence of epithelial lesions such as desquamation or stratification. However, the mucus layer was intermingled with secretory granules of goblet cell with the mucus layer spread over glycocalyx. The results of this study indicate that sodium hyaluronate injected into the subconjunctigval layer has little effect on the glycosaminoglycans density over the conjunctival epithelium in rabbit.

5-fluorouracil이 결막fibronectin에 미치는 영향 : Immune Electron Microscopic Findings 배양하 면역전자현미경소견

최준규,송기영,고명규 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

The success of the filtering in glaucoma is dependent n the patency of the outflow pathway of the aqueous humor from the anterior chamber to the filtering bleb. This is influenced by many factors such as surgical technique, physiologic and pharmacologic factors. Most of the failure of the filtering bleb formation has resulted from the scar formation of teh bleb. This is mainly derived from the fibroblast proliferation which blocks the patent bleb site. The purpose of this study is to investigate the effect of the 5-FU on the extracellular matrix. This involves mainly fibronectin excreted from in vitro, by means of the immunocytochemical stain, at the ultrastructural level. The rabbit conjunctival fibroblast and blood vessel inteh conjunctival stroma were excised and placed in a culture dish containing D-MEM and fetal calf seru. After 2nd week cultivation in 5% CO₂, and 95% air humidified at 37℃, the 5-FU(0.5mg/L) was added. At the 4th week,the culture was terminated for immunocytochemical electron microscopy. The tissue was stained with antifibronectin antibody, followed by incubation with biotin-conjugated goat anti-rabbit IgG. After labelled with HRP-labelled avidin, the tissue was stained with Diaminobenzidine. The fibroblast in the control group showed an indented nucleus with prominent nucleoli, and numerous cytoplasmic inclusions within the cytoplasm. In addition, the cytoplasmic surfacr showed numerous villous processes. However, the plasma membrane was ruptured, and the microorganelles underwent degenerative changes in the fibroblast treated with the 5-FU. The immunoelectorn microscopy revealed an absent or weak reaction product on the vascular wall and plasma membrane of the fibroblast in the 5-FU treated specimen. In short, the basement membrane of the 5-FU added cultured cel revealed a reaction product that was faint when compared with the strong reaction product in the control group. These results suggest, that 5-FU, in a cultured fibroblast and vessel of the conjunctiva, strongly suppresses the excretion of the extracellular matrix, particularly fibronectin. These results suggest, that the application of 5-FU in a filtering operation may be useful for the success of glaucoma surgery, by preventing the secretion of the extracellular matrix, particularly fibronectin.

Distribution of Proteoglycans in the Human Trabecular Meshwork: Histochemical Electron Microscopic Findings with Cuprolinic Blue

Myung Kyoo Ko,Dong Seob Kim,Jung Chul Shin 대한안과학회 1999 Korean Journal of Ophthalmology Vol.13 No.1

The distribution of sulfated proteoglycans (PGs) in the normal human trabecular meshwork was studied by histochemical electron microscopy using the cationic dye, cuprolinic blue (CB).. The trabecular meshwork was obtained from human enucleated eyes and incubated for three days. After incubation, they were stained with 0.2% CB at a critical electrolyte concentration and prepared for histochemical electron microscopy. Ultrastructurally, PG-CB complexes were found as small punctate or filamentous structures, and were associated with collagen fibrils in the cores of the trabecular beams and the basal laminae of trabecular endothelial cells. In addition, large filamentous PG-CB complexes were mainly associated with areas of amorphous extracellular matrix between the collagen fiber bundles and in the fine fibrillar material near the basal laminae of endothelial cells of Schlemm’s canal. This investigation resulted in an illustration of the ultrastructural distribution of PGs in the human trabecular meshwork. Further studies will be needed to specify the nature of PGs and their role in the aqueous outflow system.

Extracellular Matrix of the Human Retinal Pigment Epitheelial Cells in Vitro

Ko, Myung Kyoo,Choe, Joon Kiu KJO 1988 Korean Journal of Ophthalmology Vol.2 No.2

배양된 각막 실질세포의 미세구조

고명규(Myung Kyoo Ko),김동철(Dong Cheol Kim) 대한안과학회 1987 대한안과학회지 Vol.28 No.2

사람태아에서 망막의 발육과정

고명규(Myung Kyoo Ko),지제근(Je G. Chi) 대한안과학회 1989 대한안과학회지 Vol.30 No.2