http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Isolation of an exopolysaccharide-producing heavy metal-resistant Halomonas sp. MG
Govarthanan, Muthusamy,Shim, Jaehong,Praburaman, Loganathan,Kim, Seol Ah,Oh, Byung-Taek Springer-Verlag 2016 Archives of microbiology Vol.198 No.2
<P>An exopolysaccharide (EPS)-producing heavy metal-resistant Gram-negative bacterium was isolated from ore-contaminated soil. The selected strain was identified by 16S rDNA sequencing and designated as Halomonas sp. MG. Phylogenetic analysis of the gene sequence showed its close similarity with Halomonas sp. Field emission scanning electron microscopy analysis revealed that the EPS had a porous structure with small pores. X-ray diffractograms showed the non-crystalline nature of the EPS. Further, FTIR spectroscopic analysis revealed the presence of carboxyl, hydroxyl and amide groups corresponding to a typical EPS.</P>
Optimization of As Bioleaching by Herbaspirillum sp. GW103 Coupled with Coconut Oil Cake
Muthusamy Govarthanan,Loganathan Praburaman,김진원,오새강,Seralathan Kamala-Kannan,오병택 한국지하수토양환경학회 2015 지하수토양환경 Vol.20 No.2
The objective of this study was to optimize the experimental conditions for bioleaching of arsenic (As) using Herbaspirillum sp. GW103 and to understand the interaction between bacteria and As during bioleaching. Five variables, temperature, time, CaCO3, coconut oil cake, and shaking rate, were optimized using response surface methodology (RSM) based Box-Behnken design (BBD). Maximum (73.2%) bioleaching of As was observed at 30ºC, 60 h incubation, 1.75% CaCO3, 3% coconut oil cake, and 140 rpm. Sequential extraction of bioleached soil revealed that the isolate Herbaspirillum sp. GW103 significantly reduced 28.6% of water soluble fraction and increased 38.8% of the carbonate fraction. The results of the study indicate that the diazotropArsenic, Bioleaching, Box-Behnken design, Herbaspirillum sp., Heavy metals fractionhic bacteria Herbaspirillum sp. could be used for bioleaching As from mine soil. The objective of this study was to optimize the experimental conditions for bioleaching of arsenic (As) using Herbaspirillum sp. GW103 and to understand the interaction between bacteria and As during bioleaching. Five variables, temperature, time, CaCO3, coconut oil cake, and shaking rate, were optimized using response surface methodology (RSM) based Box-Behnken design (BBD). Maximum (73.2%) bioleaching of As was observed at 30ºC, 60 h incubation, 1.75% CaCO3, 3% coconut oil cake, and 140 rpm. Sequential extraction of bioleached soil revealed that the isolate Herbaspirillum sp. GW103 significantly reduced 28.6% of water soluble fraction and increased 38.8% of the carbonate fraction. The results of the study indicate that the diazotrophic bacteria Herbaspirillum sp. could be used for bioleaching As from mine soil.
Optimization of As Bioleaching by Herbaspirillum sp. GW103 Coupled with Coconut Oil Cake
Govarthanan, Muthusamy,Praburaman, Loganathan,Kim, Jin-Won,Oh, Sae-Gang,Kamala-Kannan, Seralathan,Oh, Byung-Taek Korean Society of Soil and Groundwater Environment 2015 지하수토양환경 Vol.20 No.2
The objective of this study was to optimize the experimental conditions for bioleaching of arsenic (As) using Herbaspirillum sp. GW103 and to understand the interaction between bacteria and As during bioleaching. Five variables, temperature, time, CaCO<sub>3</sub>, coconut oil cake, and shaking rate, were optimized using response surface methodology (RSM) based Box-Behnken design (BBD). Maximum (73.2%) bioleaching of As was observed at 30℃, 60 h incubation, 1.75% CaCO<sub>3</sub>, 3% coconut oil cake, and 140 rpm. Sequential extraction of bioleached soil revealed that the isolate Herbaspirillum sp. GW103 significantly reduced 28.6% of water soluble fraction and increased 38.8% of the carbonate fraction. The results of the study indicate that the diazotrophic bacteria Herbaspirillum sp. could be used for bioleaching As from mine soil.
Govarthanan, Muthusamy,Selvankumar, Thangasamy,Manoharan, Koildhasan,Rathika, Rajiniganth,Shanthi, Kuppusamy,Lee, Kui-Jae,Cho, Min,Kamala-Kannan, Seralathan,Oh, Byung-Taek Dove Medical Press 2014 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.9 No.-
<P>Synthesis of silver nanoparticles (AgNPs) with biological properties is of vast significance in the development of scientifically valuable products. In the present study, we describe simple, unprecedented, nontoxic, eco-friendly, green synthesis of AgNPs using an Indian traditional farming formulating agent, panchakavya. Silver nitrate (1 mM) solution was mixed with panchakavya filtrate for the synthesis of AgNPs. The nanometallic dispersion was characterized by surface plasmon absorbance measuring 430 nm. Transmission electron microscopy showed the morphology and size of the AgNPs. Scanning electron microscopy–energy-dispersive spectroscopy and X-ray diffraction analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy analysis revealed that proteins in the panchakavya were involved in the reduction and capping of AgNPs. In addition, we studied the antibacterial activity of synthesized AgNPs. The synthesized AgNPs (1–4 mM) extensively reduced the growth rate of antibiotic resistant bacteria such as <I>Aeromonas</I> sp., <I>Acinetobacter</I> sp., and <I>Citrobacter</I> sp., according to the increasing concentration of AgNPs.</P>
Govarthanan, Muthusamy,Kamala-Kannan, Seralathan,Kim, Seol Ah,Seo, Young-Seok,Park, Jung-Hee,Oh, Byung-Taek Springer-Verlag 2016 Archives of microbiology Vol.198 No.8
<P>Phytoremediation is an in situ, low-cost strategy for cleanup of the sites contaminated with heavy metals. Experiments were conducted to assess the impact of synthetic chelators and plant growth-promoting rhizosphere bacteria (Herbaspirillum sp. GW103) on heavy metal lead (Pb) uptake in Z. mays cultivated in Pb-contaminated soil. The present study investigated the Pb phytoaccumulation rate and plant antioxidant enzyme activities in Z. mays exposed to 100 mg/kg of PbNO3. The combination of gluconic acid (GA) with Herbaspirillum sp. GW103 treatment showed higher Pb solubility (18.9 mg/kg) compared with other chelators. The chemical chelators showed the significant difference in phytoaccumulation as well as antioxidant enzyme activities. The antioxidant enzymes such as catalase, peroxidase and superoxide dismutase activities changed under Pb stress. The study indicated that increased activity of antioxidant enzymes may play as signal inducers to fight against Pb.</P>
Govarthanan, Muthusamy,Cho, Min,Park, Jung-Hee,Jang, Jum-Suk,Yi, Young-Joo,Kamala-Kannan, Seralathan,Oh, Byung-Taek Hindawi Limited 2016 Journal of nanomaterials Vol.2016 No.-
<P>Agroindustrial byproduct mediated green synthesis of silver nanoparticles was carried out using cottonseed oilcake (CSOC) extract. The aqueous silver nitrate formed stable silver nanoparticles with CSOC extract as a reducing agent for Ag<SUP>+</SUP>to Ag<SUP>0</SUP>. The synthesized nanoparticles were characterized using energy dispersive X-ray spectroscopy (EDS), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, and X-ray diffraction (XRD) techniques. The synthesized silver nanoparticles (AgNPs) (4 mM) significantly inhibited the growth of phytopathogens,<I>Pseudomonas syringae</I>pv.<I>actinidiae</I>and<I>Ralstonia solanacearum</I>. Further, cytotoxicity of AgNPs was evaluated using rat splenocyte cells. The splenocyte viability was decreased according to the increasing concentration of AgNPs and 90% of cell death was observed at 100 <I>μ</I>g/mL.</P>
Govarthanan, Muthusamy,Park, Jung-Hee,Praburaman, Loganathan,Yi, Young-Joo,Cho, Min,Myung, Hyun,Gnanendra, Shanmugam,Kamala-Kannan, Seralathan,Oh, Byung-Taek Springer-Verlag 2015 Current microbiology Vol.71 No.3
<P>This study investigated the expression rate and molecular modeling of Wzb gene, a low molecular weight protein tyrosine phosphatase, under As stress in Herbaspirillum sp. GW103. Expression of Wzb gene was quantified at transcriptional level through real-time quantitative PCR. The results showed up- and down-regulations of Wzb gene in the presence of As (50 and 100?mg/L). The maximum Wzb transcript expression was 1.2-fold after 72?h exposure to 50?mg/L of As. However, the minimum expression was 0.1-fold after 48?h exposure to 100?mg/L of As. The Wzb protein sequence was retrieved from NCBI sequence database and was used for in silico analysis. 3D structure of Wzb gene was predicted by comparative modeling using modeler 9v9. Further, the model was validated for its quality by Ramachandran plot, ERRAT, Verify 3D, and SAVES server which revealed structure and quality of the Wzb gene model.</P>
Muthusamy Govarthanan,카말라칸,Thangasamy Selvankumar,Kandasamy Selvam,Chinnappan Sudhakar,Vincent Aroulmoji 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.27 No.-
A native feather-degrading keratinolytic bacterium, Bacillus sp. MG-MASC-BT was isolated from featherdumping soil in Mallasamudram, Tamil Nadu, India and screened for keratinase production using alkalitreated horn waste (HW) and feather waste (FW). The study factors influencing keratinase productionwas optimized by Box–Behnken design (BBD). The maximum enzyme production (1075 U/ml) wasobserved at pH 7.0, temperature 55 8C and growth period of 60 h. The media supplemented with 6% ofHW and FW enhanced keratinase production. Statistics based contour plots were generated to evaluatethe changes in the response surface and to understand the relationship between the enzyme yield andthe culture conditions.
Aravinthan, Adithan,Govarthanan, Muthusamy,Selvam, Kandasamy,Praburaman, Loganathan,Selvankumar, Thangasamy,Balamurugan, Rangachari,Kamala-Kannan, Seralathan,Kim, Jong-Hoon Dove Medical Press 2015 INTERNATIONAL JOURNAL OF NANOMEDICINE Vol.10 No.-
<P>A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of <I>Helianthus tuberosus</I> (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as <I>Ralstonia solanacearum</I> and <I>Xanthomonas axonopodis</I>. The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.</P>