Synthesis, characterization and evaluation of antioxidant and anticancer activities of novel benzisoxazole-substituted-allyl derivatives

Mohanam Anand,Muthukaruppan Alagar,Vaithialingam Selvaraj 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.4

A novel series of various 2-allylbenzo[d]isoxazol-3(2H)-ones were synthesized using benzo[d]isoxazol-3(2H)-one treated with different allyl bromides/chlorides in the presence of water-mediated cesium carbonate as a newcatalyst 3(a-h). The structures of the newly synthesized Benzisoxazole-substituted-allyl derivatives were characterizedby spectroscopic methods and mass spectrometry. These synthesized compounds were evaluated for their in vitro antioxidantand anticancer activity. Compounds 3b, d, f, h were identified as the best hit against HT-29 Human colon cancercells. Similarly, compounds like 3b, d, f, h showed significant antioxidant activity compared to the standard drug butylatedhydroxy toluene (BHT).

Synthesis of <i>Oldenlandia umbellata</i> stabilized silver nanoparticles and their antioxidant effect, antibacterial activity, and bio-compatibility using human lung fibroblast cell line WI-38

Subramanian, Palanisamy,Ravichandran, Anjali,Manoharan, Vinosha,Muthukaruppan, Reka,Somasundaram, Selvakumar,Pandi, Boomi,Krishnan, Anand,Marimuthu, Prabhu Narayanasamy,Somasundaram, Selliah Swamy Nat Elsevier 2019 Process biochemistry Vol.86 No.-

<P><B>Abstract</B></P> <P>In the past few years, green synthesized silver nanoparticles (AgNPs) are one of predominantly used materials in therapeutics and clinical practices. In this study, AgNPs were synthesized from aqueous extract of <I>Oldenlandia umbellata</I> and investigated their pharmaceutical properties. UV–vis spectroscopy of <I>O. umbellata</I> mediated silver nanoparticles (AgNPs@OUa) showed a peak at 423 nm. Fourier transform infrared spectroscopy and phytochemical screening explained the contribution of biomolecules in AgNPs@OUa synthesis. Field emission scanning electron microscope and high-resolution transmission electron microscope examination revealed that small monodispersed spherical nanoparticles had an average size of 22.7 nm. Antioxidant assays were used to examine antioxidant properties of AgNPs@OUa in comparison with <SMALL>L-</SMALL>ascorbic acid (Vitamin C) as a standard antioxidant. AgNPs@OUa showed dose-dependent antioxidant activities. Antibacterial activities of AgNPs@OUa exhibited the highest zone of inhibition against <I>Streptococcus mutans</I> and <I>Escherichia coli</I> were 16.03 ± 0.35 mm and 19.76 ± 0.25 mm, respectively, at concentration of 1.0 mg L<SUP>−1</SUP>. Biocompatibility of AgNPs@OUa against human lung fibroblast (WI-38) cell line was also tested. Even though, the cells treated with the uppermost concentration (100 μg/mL) of AgNPs@OUa, it enhances the cell viability (∼90%), suggesting that AgNPs@OUa had no toxicity with excellent biocompatibility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> First report on the bio synthesis of AgNPs using <I>O. umbellata</I> aqueous extract. </LI> <LI> The AgNPs@OUa were characterized by spectral and microscopy analysis. </LI> <LI> AgNPs@OUa were act as a significant antioxidant and antibacterial activities. </LI> <LI> AgNPs@OUa showed excellent biocompatibility against WI-38 cell lines. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Electrocatalytic Reduction of Hydrogen Peroxide on Silver Nanoparticles Stabilized by Amine Grafted Mesoporous SBA-15

Mari Vinoba,정순관,Margandan Bhagiyalakshmi,Muthukaruppan Alagar 대한화학회 2010 Bulletin of the Korean Chemical Society Vol.31 No.12

Mesoporous SBA-15 was synthesized using tetraethylorthosilicate (TEOS) as the silica source and Pluronic (P123) as the structure-directing agent. The defective Si-OH groups present in SBA-15 were successively grafted with 3-chloropropyltrimethoxysilane (CPTMS) followed by tris-(2-aminoethyl) amine (TAEA) and/or tetraethylenepentamine (TEPA) for effective immobilization of silver nanoparticles. Grafting of TAEA and/or TEPA amine and immobilization of silver nanoparticles inside the channels of SBA-15 was verified by XRD, TEM, IR and BET techniques. The silver nanoparticles immobilized on TAEA and /or TEPA grafted SBA-15 was subjected for electrocatalytic reduction of hydrogen peroxide (H2O2). The TEPA stabilized silver nanoparticles show higher efficiency for reduction of H2O2 than that of TAEA,due to higher number of secondary amine groups present in TEPA. The amperometric analysis indicated that both the Ag/SBA-15/TAEA and Ag/SBA-15/TEPA modified electrodes required lower over-potential and hence possess high sensitivity towards the detection of H2O2. The reduction peak currents were linearly related to hydrogen peroxide concentration in the range between 3 × 10‒4 M and 2.5 × 10‒3 M with correlation coefficient of 0.997 and detection limit was 3 × 10‒4 M.

Immobilization of carbonic anhydrase-gold nanoparticle on mesoporous silica: Its nanobiocatalytic activity for hydrolysis of p-nitrophenyl acetate

( Mari Vinoba ),김대훈,임경수,정순관,( Muthukaruppan Alagar ) 한국공업화학회 2010 한국공업화학회 연구논문 초록집 Vol.2010 No.1

Enhancement of biogas production from microalgal biomass through cellulolytic bacterial pretreatment

Kavitha, S.,Subbulakshmi, P.,Rajesh Banu, J.,Gobi, Muthukaruppan,Tae Yeom, Ick Elsevier 2017 Bioresource technology Vol.233 No.-

<P><B>Abstract</B></P> <P>Generation of bioenergy from microalgal biomass has been a focus of interest in recent years. The recalcitrant nature of microalgal biomass owing to its high cellulose content limits methane generation. Thus, the present study investigates the effect of bacterial-based biological pretreatment on liquefaction of the microalga <I>Chlorella vulgaris</I> prior to anaerobic biodegradation to gain insights into energy efficient biomethanation. Liquefaction of microalgae resulted in a higher biomass stress index of about 18% in the experimental (pretreated with cellulose-secreting bacteria) vs. 11.8% in the control (non-pretreated) group. Mathematical modelling of the biomethanation studies implied that bacterial pretreatment had a greater influence on sustainable methane recovery, with a methane yield of about 0.08 (g Chemical Oxygen Demand/g Chemical Oxygen Demand), than did control pretreatment, with a yield of 0.04 (g Chemical Oxygen Demand/g Chemical Oxygen Demand). Energetic analysis of the proposed method of pretreatment showed a positive energy ratio of 1.04.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Microalgal biomass pretreatment by bacteria enhances liquefaction of about 18%. </LI> <LI> Bacterial pretreatment increases the macromolecular release considerably. </LI> <LI> Experimental microalgae improves the methane to 0.08gCOD/gCOD comparing to control. </LI> <LI> Methane production rate increased with hydrolysis constant of about 0.24day<SUP>−1</SUP>. </LI> <LI> A positive energy ratio of about 1.04 was achieved in experimental microalgae. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Immobilization of Human Carbonic Anhydrase on Gold Nanoparticles Assembled onto Amine/Thiol-Functionalized Mesoporous SBA-15 for Biomimetic Sequestration of CO₂

Vinoba, Mari,Lim, Kyoung Soo,Lee, Si Hyun,Jeong, Soon Kwan,Alagar, Muthukaruppan American Chemical Society 2011 Langmuir Vol.27 No.10

<P>A biocatalyst was synthesized by immobilizing human carbonic anhydrase onto gold nanoparticles assembled over amine/thiol-functionalized mesoporous SBA-15. The physicochemical properties of the functionalized mesoporous SBA-15 were obtained by XRD, BET, FE SEM, HR TEM, EDS, and zeta potential analysis. The biocatalytic performance was studied for <I>para</I>-nitrophenyl acetate (p-NPA) hydrolysis. The kinetic parameters <I>K</I><SUB>m</SUB> were found to be 22.35 and 27.75 mM, and <I>K</I><SUB>cat</SUB>/<I>K</I><SUB>m</SUB> values were 1514.09 and 1612.25 M<SUP>−1</SUP> s<SUP>−1</SUP> for HCA immobilized on gold nanoparticles assembled on amine/thiol-functionalized mesoporous SBA-15 (HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15), respectively. These HCA/Au/APTES/SBA-15 and HCA/Au/MPTES/SBA-15 were investigated for biocatalytic hydration of CO<SUB>2</SUB> and its precipitation as CaCO<SUB>3</SUB>. The amount of CaCO<SUB>3</SUB> precipitated over HCA/Au/MPTES/SBA-15 was nearly the same as that precipitated over free HCA. Storage stability and reusability studies suggested that HCA/Au/MPTES/SBA-15 retained its activity even after 20 days storage at 25 °C and 20 recycling runs. The present results demonstrate that HCA/Au/MPTES/SBA-15 and HCA/Au/APTES/SBA-15 are highly efficient potential nanobiocatalysts for industrial-scale CO<SUB>2</SUB> sequestration.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2011/langd5.2011.27.issue-10/la105029h/production/images/medium/la-2010-05029h_0011.gif'></P>

Electrocatalytic Reduction of Hydrogen Peroxide on Silver Nanoparticles Stabilized by Amine Grafted Mesoporous SBA-15

Vinoba, Mari,Jeong, Soon-Kwan,Bhagiyalakshmi, Margandan,Alagar, Muthukaruppan Korean Chemical Society 2010 Bulletin of the Korean Chemical Society Vol.31 No.12

Mesoporous SBA-15 was synthesized using tetraethylorthosilicate (TEOS) as the silica source and Pluronic (P123) as the structure-directing agent. The defective Si-OH groups present in SBA-15 were successively grafted with 3-chloropropyltrimethoxysilane (CPTMS) followed by tris-(2-aminoethyl) amine (TAEA) and/or tetraethylenepentamine (TEPA) for effective immobilization of silver nanoparticles. Grafting of TAEA and/or TEPA amine and immobilization of silver nanoparticles inside the channels of SBA-15 was verified by XRD, TEM, IR and BET techniques. The silver nanoparticles immobilized on TAEA and /or TEPA grafted SBA-15 was subjected for electrocatalytic reduction of hydrogen peroxide ($H_2O_2$). The TEPA stabilized silver nanoparticles show higher efficiency for reduction of $H_2O_2$ than that of TAEA, due to higher number of secondary amine groups present in TEPA. The amperometric analysis indicated that both the Ag/SBA-15/TAEA and Ag/SBA-15/TEPA modified electrodes required lower over-potential and hence possess high sensitivity towards the detection of $H_2O_2$. The reduction peak currents were linearly related to hydrogen peroxide concentration in the range between $3{\times}10^{-4}\;M$ and $2.5{\times}10^{-3}\;M$ with correlation coefficient of 0.997 and detection limit was $3{\times}10^{-4}\;M$.