An Effective Method for Deproteinization of Bioactive Polysaccharides Extracted from Lingzhi (Ganoderma atrum)

Yi Chen,Mingyong Xie,Wenjuan Li,Hui Zhang,Shaoping Nie,Yuanxing Wang,Chang Li 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1

Deproteinization procedure is a fundamental step for analyzing polysaccharide from natural plants. In this study, in the course of refining bioactive polysaccharides from lingzhi (Ganoderma atrum), an effective deproteinization method using lead acetate solution was established by comparing with other available methods. The percentages of deproteinization, polysaccharide loss, and its antioxidant activities loss were used as the index to evaluate and optimize the precipitation experimental conditions. The results showed that the modified method, precipitation with the addition of 0.4-0.52%(w/v) lead acetate, was superior to the others, as evidenced by the highest deproteinization efficiency (88%), as well as the lowest polysaccharide loss (17%). And notably its antioxidant activity also remained good (loss 15%). It provides a simple prefractionation step for the analysis of polysaccharide from natural plants. Polysaccharide isolated by this method is in the native state. Our method may offer a rapid method for removing protein from plant polysaccharides in large scale.

Antioxidant and antibacterial capabilities of phenolic compounds and organic acids from Camellia oleifera cake

Duoduo Zhang,Shaoping Nie,Mingyong Xie,Jielun Hu 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.1

There is growing interest in the antioxidants and antibacterial activity from natural substances. The purpose of the research was to gain and distinguish phenolic substances and organic acids in the Camellia oleifera cake, and to study their antioxidant and antibacterial activities. The extraction and purification of them were achieved by solvent extraction and column separation, respectively. The conclusions displayed that purity of the phenolic substances was 94.1 ± 0.5% w/w and that of organic acid was 96.0 ± 0.3% w/w; Fifteen phenolic substances were certificated using HPLC–ESI–MS technology; oxalic, citric, acetic, malic, and succinic acids are discovered to be main organic acids. In addition, the phenolic substances and organic acids both have good antioxidant activity and obvious inhibition against six species of bacteria. These conclusions can be useful in the reuse of the waste of Camellia oleifera oil industry in the future.

PPNC: Privacy Preserving Scheme for Random Linear Network Coding in Smart Grid

( Shiming He ),( Weini Zeng ),( Kun Xie ),( Hongming Yang ),( Mingyong Lai ),( Xin Su ) 한국인터넷정보학회 2017 KSII Transactions on Internet and Information Syst Vol.11 No.3

In smart grid, privacy implications to individuals and their families are an important issue because of the fine-grained usage data collection. Wireless communications are utilized by many utility companies to obtain information. Network coding is exploited in smart grids, to enhance network performance in terms of throughput, delay, robustness, and energy consumption. However, random linear network coding introduces a new challenge for privacy preserving due to the encoding of data and updating of coefficients in forwarder nodes. We propose a distributed privacy preserving scheme for random linear network coding in smart grid that considers the converged flows character of the smart grid and exploits a homomorphic encryption function to decrease the complexities in the forwarder node. It offers a data confidentiality privacy preserving feature, which can efficiently thwart traffic analysis. The data of the packet is encrypted and the tag of the packet is encrypted by a homomorphic encryption function. The forwarder node random linearly codes the encrypted data and directly processes the cryptotext tags based on the homomorphism feature. Extensive security analysis and performance evaluations demonstrate the validity and efficiency of the proposed scheme.

Polysaccharide from the Seeds of Plantago asiatica L. Protect Against Lipopolysaccharide-Induced Liver Injury

Fenfen Li,Danfei Huang,Shaoping Nie,Mingyong Xie 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.10

Previous studies have proven that polysaccharide obtained from the seeds of Plantago asiatica L. (PLCP) could induce maturation of murine dendritic cells, promote defecation, and possess antioxidant activity in vitro. However, the effect of PLCP on lipopolysaccharide (LPS)-induced liver injury in mice has been rarely reported. In this study, we investigated the anti-inflammatory effect of PLCP on LPS-induced liver injury. Mice were pretreated orally with different dose of PLCP for 3 weeks. On day 22, they were injected intraperitoneally with LPS and sacrificed 12 h later. The results showed that PLCP inhibited the excessive production of tumor necrosis factor-α, interleukin (IL)-6, IL-10, IL-2, and IL-1β in mouse serum and liver. PLCP also improved glutathione peroxidase and total antioxidant capacity activities in mouse liver. In addition, PLCP inhibited nitric oxide, inducible nitric oxide synthase, and cyclooxygenase-2 expression, and increased metallothionein production in mouse liver. Consequently, PLCP may possess protective effects on inflammatory associated liver injury.

Are Chinese Edible Oils Safe? A Survey of Trans Fatty Acid Contents in Chinese Edible Oils

Xiaojun Huang,Shaoping Nie,Meiyan Yang,Jianhua Xie,Chang Li,Mingyong Xie 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.2

Nine different edible oil types from 7 cities in China were analyzed. Trans fatty acid (TFA) contents in edible oils were determined, TFA distribution characteristics were analyzed, and a risk assessment was performed. Total TFA contents varied from 0.075±0.011 to 2.243±0.770 g/100 g of oil, with a mean content of 0.66 g/100 g of oil. TFA contents differed among different oil types and among different cities. Generally speaking, oil samples from northern areas of China contained more TFA, and lard usually had higher levels of TFA than other oils. An exposure assessment showed that the estimated daily intake of TFA from Chinese edible oils was low, and a risk assessment indicated that there was a low risk for TFA consumption in Chinese edible oils.

Comparison of structural features and antioxidant activity of polysaccharides from natural and cultured Cordyceps sinensis

Junqiao Wang,Shaoping Nie,Lijiao Kan,Haihong Chen,Steve W. Cui,Aled O. Phillips,Glyn O. Phillips,Mingyong Xie 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.1

Four polysaccharides (named as P1, P2, and P3 from three natural Cordyceps sinensis and P4 from cultured C. sinensis) were obtained by hot-water extraction and ethanol precipitation and their structural characteristics as well as antioxidant potentials were compared. Results revealed that the backbone of P1, P2, and P3 comprised α-1,4-glucose, with a branching point mainly at position 6 and terminating at glucose. On the other hand, the structure of P4 was highly complex, mainly comprising glucose, galactose, and mannose, with 1,4-glucose and 1,4-galactose as the main chain. For in vitro antioxidant assays, all the four polysaccharides showed similar scavenging capacity against DPPH and hydroxyl radicals, whereas P1 had a relatively low ferric reducing ability, possibly related to a combination of factors such as the phenolic compounds and amino acids that conjugated in polysaccharides.

Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets

Kong, Xiangfeng,Yin, Yulong,Wu, Guoyao,Liu, Hejun,Yin, Fugui,Li, Tiejun,Huang, Ruilin,Ruan, Zheng,Xiong, Hua,Deng, Zeyuan,Xie, Mingyong,Liao, Yiping,Kim, Sungwoo Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.9

This study was conducted to test the hypothesis that dietary supplementation with an herbal extract of Acanthopanax senticosus (AS) enhances the immune response in weaned piglets. Sixty piglets weaned at 21 days of age were randomly assigned to 3 treatment groups representing the addition of 0 or 1 g/kg of the AS extract or 0.2 g/kg of colistin (an antibiotic) to maize- and soybean meal-based diets (n = 20 per group). On days 7, 14 and 28 after initiation of the addition, total and differential counts of leucocytes, proliferating activity of peripheral lymphocytes, serum levels of immunoglobulins (Ig) and cytokines and the spleen index were determined. The AS extract decreased (p<0.05) the number of neutrophils on days 7 and 28 in comparison with the control group and reduced (p<0.05) serum interleukin-$1{\beta}$ level on day 28 compared with the other 2 groups. Dietary supplementation with the AS extract increased (p<0.05) the lymphocyte/leukocyte ratio on day 28 compared with the control group and increased the proliferating activity of lymphocytes on days 14 and 28 compared with the other 2 groups. The AS extract increased (p<0.05) the serum content of IgG on day 7 and of IgG and IgM on day 28 compared with the other 2 groups, as well as increasing the serum content of tumor necrosis factor on day 7 and spleen index on days 7 and 28 compared with the control group. Collectively, these findings suggest that the AS extract as a dietary additive enhances the cellular and humoral immune responses of weaned piglets by modulating the production of immunocytes, cytokines and antibodies.