Knockout of Myostatin by Zinc-finger Nuclease in Sheep Fibroblasts and Embryos

Zhang, Xuemei,Wang, Liqin,Wu, Yangsheng,Li, Wenrong,An, Jing,Zhang, Fuchun,Liu, Mingjun Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.10

Myostatin (MSTN) can negatively regulate the growth and development of skeletal muscle, and natural mutations can cause "double-muscling" trait in animals. In order to block the inhibiting effect of MSTN on muscle growth, we transferred zinc-finger nucleases (ZFN) which targeted sheep MSTN gene into cultured fibroblasts. Gene targeted colonies were isolated from transfected fibroblasts by serial dilution culture and screened by sequencing. Two colonies were identified with mono-allele mutation and one colony with bi-allelic deletion. Further, we introduced the MSTN-ZFN mRNA into sheep embryos by microinjection. Thirteen of thirty-seven parthenogenetic embryos were targeted by ZFN, with the efficiency of 35%. Our work established the technical foundation for generation of MSTN gene editing sheep by somatic cloning and microinjection ZFN into embryos.

Prediction of flow boiling heat transfer coefficient in horizontal channels varying from conventional to small-diameter scales by genetic neural network

Jing Zhang,Yichao Ma,Mingjun Wang,Dalin Zhang,Suizheng Qiu,Wenxi Tian,Guanghui Su 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.8

Three-layer back propagation network (BPN) and genetic neural network (GNN) were developed in this study to predict the flow boiling heat transfer coefficient (HTC) in conventional and small-diameter channels. The GNN has higher precision than BPN (with root mean square errors of 17.16% and 20.50%, respectively) and other correlations. The inputs include vapor quality x, mass flux G, heat flux q, diameter D and physical parameter φ, and the predicted flow boiling HTC is set as the outputs. Influences of input parameters on the flow boiling HTC are discussed based on the trained GNN: nucleate boiling promoted by a larger saturated pressure, a larger heat flux and a smaller diameter is dominant in small channels; convective boiling improved by a larger mass flux and a larger vapor quality is more significant in conventional channels. The HTC increases with pressure both in conventional and small channels. The HTC in conventional channels rises when mass flux increases but remains almost unaffected in small channels. A larger heat flux leads to the HTC growth in small channels and an increase of HTC was observed in conventional channels at a higher vapor quality. HTC increases inversely with diameter before dry out.

Vapor-phase selective hydrogenation of maleic anhydride to succinic anhydride over Ni/TiO2 catalysts

Weitao Huo,Chunlei Zhang,Hongjing Yuan,Mingjun Jia,Chunli Ning,Yong Tang,Yi Zhang,Jiahuan Luo,Zhenlu Wang,Wenxiang Zhang 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6

A series of supported Ni/TiO2 catalysts were prepared by incipient wetness impregnation method underdifferent calcination temperatures, and the as-prepared catalysts were characterized by X-ray diffraction(XRD), hydrogen temperature-programmed reduction (H2-TPR) and X-ray photoelectron spectroscopy(XPS). The catalytic properties of these Ni/TiO2 catalysts were investigated in the vapor phasehydrogenation of maleic anhydride (MA) to succinic anhydride (SA). The results showed that thecatalytic activity and the selectivity of the Ni/TiO2 catalysts were strongly affected by the calcinationtemperature. The catalyst calcined at 1023 K showed a relatively higher SA selectivity of 96% at high MAconversion (96%) under the tested conditions (493 K and 0.2 MPa). The improvement of SA selectivitycould be mainly assigned to the presence of suitable metal–support interaction, which can play a role incatalytic property of active nickel species as electron promoter. Besides, the change of surface propertiesof TiO2 support with the increasing calcination temperatures, e.g., the decrease of Lewis acid sites, mightalso have some positive role in reducing the side-products like g-butyrolacetone (GBL).

The exchange bias effect and Griffiths phase in La1.5Sr0.5Co1-Ni MnO6: The impact of the divalent Ni

Zhang Hongguang,Wang Sen,Chen Wei,Wang Mingjun,Li Yongtao,Xie Liang 한국물리학회 2023 Current Applied Physics Vol.53 No.-

The effects of Co-site doping of Ni2+ ions on the crystal structure, electronic structure, and magnetic properties of La1.5Sr0.5CoMnO6 are investigated. The doping causes an increase in the anti-site disorder and a change in the content of Co2+/3+ and Mn3+/4+, as well as a change in the spin state of Co ions. Ni doping introduces Ni2+-O2-- Mn4+ ferromagnetic interactions to increase the ferromagnetic transition temperature. Samples display different dominant clusters, and field cooling promotes the presence of ferromagnetic clusters, allowing an increase in the Griffiths phase. Interestingly, Ni doping enables tuning the conventional and spontaneous exchange bias (EB) effects, increasing the EB field for intermediate concentrations. Moreover, the maxima of both EB fields do not occur in the same sample, which leads to a new understanding of the relationship between these two effects. The possible mechanisms related to the uncompensated spin and competition between ferromagnetic and antiferromagnetic clusters are discussed, respectively.

An Improved Website Structure Optimizing Algorithm

Mingjun Li,Mingxin Zhang,Jinlong Zheng,Ying Lu 보안공학연구지원센터 2013 International Journal of Smart Home Vol.7 No.3

Effects of vertebral number variations on carcass traits and genotyping of Vertnin candidate gene in Kazakh sheep

Zhang, Zhifeng,Sun, Yawei,Du, Wei,He, Sangang,Liu, Mingjun,Tian, Changyan Asian Australasian Association of Animal Productio 2017 Animal Bioscience Vol.30 No.9

Objective: The vertebral number is associated with body length and carcass traits, which represents an economically important trait in farm animals. The variation of vertebral number has been observed in a few mammalian species. However, the variation of vertebral number and quantitative trait loci in sheep breeds have not been well addressed. Methods: In our investigation, the information including gender, age, carcass weight, carcass length and the number of thoracic and lumbar vertebrae from 624 China Kazakh sheep was collected. The effect of vertebral number variation on carcass weight and carcass length was estimated by general linear model. Further, the polymorphic sites of Vertnin (VRTN) gene were identified by sequencing, and the association of the genotype and vertebral number variation was analyzed by the one-way analysis of variance model. Results: The variation of thoracolumbar vertebrae number in Kazakh sheep (18 to 20) was smaller than that in Texel sheep (17 to 21). The individuals with 19 thoracolumbar vertebrae (T13L6) were dominant in Kazakh sheep (79.2%). The association study showed that the numbers of thoracolumbar vertebrae were positively correlated with the carcass length and carcass weight, statistically significant with carcass length. To investigate the association of thoracolumbar vertebrae number with VRTN gene, we genotyped the VRTN gene. A total of 9 polymorphic sites were detected and only a single nucleotide polymorphism (SNP) (rs426367238) was suggested to associate with thoracic vertebral number statistically. Conclusion: The variation of thoracolumbar vertebrae number positively associated with the carcass length and carcass weight, especially with the carcass length. VRTN gene polymorphism of the SNP (rs426367238) with significant effect on thoracic vertebral number could be as a candidate marker to further evaluate its role in influence of thoracolumbar vertebral number.

A QoS Constraints Location-based Services Selection Model and Algorithm under Mobile Internet Environment

Mingjun Xin,Tianle Jiang,Rui Zhang 보안공학연구지원센터(IJGDC) 2014 International Journal of Grid and Distributed Comp Vol.7 No.2

Distribution Characteristics of Grassland Coverage Change in Tibetan Plateau Since 1982

Mingjun DING,Yi-Li Zhang,Linshan LIU 대한지리학회 2011 대한지리학회 컨퍼런스 자료집 Vol.- No.6

Effects of maize straw treated with various levels of CaO and moisture on composition, structure, and digestion by in vitro gas production

Shi Mingjun,Ma Zhanxia,Tian Yujia,Zhang Xuewei,Shan Huiyong 아세아·태평양축산학회 2021 Animal Bioscience Vol.34 No.12

Objective: The objective of this study was to explore the effects of maize straw treated with calcium oxide (CaO) and various moisture, on the composition and molecular structure of the fiber, and gas production by fermentation in an in vitro rumen environment. Methods: The experiment used 4×3 Factorial treatment. Maize straws were treated with 4 concentrations of CaO (0%, 3%, 5%, and 7% of dry straw weight) and 3 moisture contents (40%, 50%, and 60%). Scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray fluorescence spectroscopy were employed to measure the surface texture, secondary molecular structure of carbohydrate, and calcium (Ca) content of the maize straw, respectively. The correlation of secondary molecular structures and fiber components of maize straw were analyzed by CORR procedure of SAS 9.2. In vitro rumen fermentation was performed for 6, 12, 24, 48, and 72 h to measure gas production. Results: Overall, the moisture factor had no obvious effect on the experimental results. Neutral detergent fiber (NDF), acid detergent fiber, acid detergent lignin, hemicellulose and cellulose contents decreased (p<0.05) with increasing concentrations of CaO treatment. Surface and secondary molecular structure of maize straw were affected by various CaO and moisture treatments. NDF had positive correlation (p<0.01) with Cell-H (H, height), Cell-A (A, area), CHO-2-H. Hemicellulose had positive correlation (p<0.01) with Lignin-H, Lignin-A, Cell-H, Cell-A. Ca content of maize straw increased as the concentration of CaO was increased (p<0.01). Gas production was highest in the group treated with 7% CaO. Conclusion: CaO can adhere to the surface of the maize straw, and then improve the digestibility of the maize straw in ruminants by modifying the structure of lignocellulose and facilitating the maize straw for microbial degradation. Objective: The objective of this study was to explore the effects of maize straw treated with calcium oxide (CaO) and various moisture, on the composition and molecular structure of the fiber, and gas production by fermentation in an in vitro rumen environment.Methods: The experiment used 4×3 Factorial treatment. Maize straws were treated with 4 concentrations of CaO (0%, 3%, 5%, and 7% of dry straw weight) and 3 moisture contents (40%, 50%, and 60%). Scanning electron microscopy, Fourier transform infrared spectroscopy and X-ray fluorescence spectroscopy were employed to measure the surface texture, secondary molecular structure of carbohydrate, and calcium (Ca) content of the maize straw, respectively. The correlation of secondary molecular structures and fiber components of maize straw were analyzed by CORR procedure of SAS 9.2. In vitro rumen fermentation was performed for 6, 12, 24, 48, and 72 h to measure gas production.Results: Overall, the moisture factor had no obvious effect on the experimental results. Neutral detergent fiber (NDF), acid detergent fiber, acid detergent lignin, hemicellulose and cellulose contents decreased (p<0.05) with increasing concentrations of CaO treatment. Surface and secondary molecular structure of maize straw were affected by various CaO and moisture treatments. NDF had positive correlation (p<0.01) with Cell-H (H, height), Cell-A (A, area), CHO-2-H. Hemicellulose had positive correlation (p<0.01) with Lignin-H, Lignin-A, Cell-H, Cell-A. Ca content of maize straw increased as the concentration of CaO was increased (p<0.01). Gas production was highest in the group treated with 7% CaO.Conclusion: CaO can adhere to the surface of the maize straw, and then improve the digestibility of the maize straw in ruminants by modifying the structure of lignocellulose and facilitating the maize straw for microbial degradation.

Analysis and research on dynamic characteristics of 3D braided composite two-stage reducer case

Weiliang Zhang,Xupeng Wang,Xiaomin Ji,Xinyao Tang,Fengfeng Liu,Shuwei Liu,Mingjun Liu,Xiao-Long Gao 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.6

Based on the analysis of the movement rule of 3D five-way braided yarn, the 3D five-way braided case model is divided into three parts: base, case bottom plate and case wall. According to the characteristics of each part, the braiding and forming principle of a 3D five-way composite case was analyzed. Under the assumption of ignoring the influence of friction, bearing and other factors on the system, the differential equations of motion of the two-stage gear transmission system with case were established. The two-stage gear transmission system was numerically analyzed by Euler method, and the influence of dimensionless meshing frequency, clearance, case mass and damping on the dynamic characteristics of the transmission system was studied. After that, high and low frequency disturbance was applied to the input case. The research shows that the damping ratio of the composite case increases and the low frequency attenuation is accelerated due to the decrease of the case mass, so the composite case has better shock resistance to low frequency interference.