Effect on Viability of Microencapsulated Lactobacillus rhamnosus with the Whey Proteinpullulan Gels in Simulated Gastrointestinal Conditions and Properties of Gels

Minghao Zhang,Dan Cai,Qiumei Song,Yu Wang,Haiyue Sun,Chunhong Piao,Hansong Yu,Junmei Liu,Jingsheng Liu,Yuhua Wang 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.3

Lactobacillus rhamnosus GG (LGG) has low resistance to low pH and bile salt in the gastrointestinal juice. In this study, the gel made from whey protein concentrate (WPC) and pullulan (PUL) was used as the wall material to prepare the microencapsulation for LGG protection. The gelation process was optimized and the properties of gel were also determined. The results showed the optimal gel was made from 10% WPC and 8.0% PUL at pH 7.5, which could get the best protective effect; the viable counts of LGG were 6.61 Log CFU/g after exposure to simulated gastric juice (SGJ) and 9.40 Log CFU/g to simulated intestinal juice (SIJ) for 4 h. Sodium dodecyl sulphite polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the WPC-PUL gel had low solubility in SGJ, but dissolved well in SIJ, which suggested that the gel can protect LGG under SGJ condition and release probiotics in the SIJ. Moreover, when the gel has highest hardness and water-holding capacity, the viable counts of LGG were not the best, suggesting the relationship between the protection and the properties of the gel was non-linear.

Whey Protein Concentrate, Pullulan, and Trehalose as Thermal Protective Agents for Increasing Viability of Lactobacillus plantarum Starter by Spray Drying

Haiyue Sun,Xiaoman Hua,Minghao Zhang,Yu Wang,Yiying Chen,Jing Zhang,Chao Wang,Yuhua Wang 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.1

It is necessary to add protective agents for protecting the probiotic viability in the preparation process of probiotics starter. In this study, we used whey protein concentrate (WPC), pullulan, trehalose, and sodium glutamate as the protective agent and optimized the proportion of protective agent and spray-drying parameters to achieve the best protective effect on Lactobacillus plantarum. Moreover, the viable counts of L. plantarum in starter stored at different temperatures (–20℃, 4℃, and 25℃) for 360 days were determined. According to response surface method (RSM), the optimal proportion of protective agent was 24.6 g/L WPC, 18.8 g/L pullulan, 16.7 g/L trehalose and 39.3 g/L sodium glutamate. The optimum spray-drying parameters were the ratio of bacteria to protective agents 3:1 (v: v), the feed flow rate 240 mL/h, and the inlet air temperature 115℃ through orthogonal test. Based on the above results, the viable counts of L. plantarum was 12.22±0.27 Log CFU/g and the survival rate arrived at 85.12%. The viable counts of L. plantarum stored at –20℃ was more than 1010 CFU/g after 200 days.

Research on Intelligent Urban Traffic Construction for Driverless

Yanlu Yang(Yanlu Yang),Yiyuan Wang(Yiyuan Wang),Minghao Gao(Minghao Gao) 동북아경상학회 2022 동북아경상연구 Vol.3 No.2

Purpose - The purpose of this paper is to explore the existing problems of the barrage video website in view of the current situation that the barrage video website greatly impacts people’s video viewing mod. Design/Methodology/Approach - Based on the subversive revolutionary impact of driverless technology on the future of urban intelligent transportation, this paper mainly adopts the method of theoretical analysis. Combined with the current urban intellectual transportation construction status, it conducts theoretical research on future urban thoughtful transportation construction planning. Findings - This study puts forward constructive suggestions on the smart construction of future cities from six aspects: top-level design, technical support, intelligent infrastructure construction, intelligent transportation decisionmaking system, industry and talent construction, and social cooperation. Research Implications - In the management of this paper, This paper is of great significance to the planning and construction of smart transportation in future cities and the coordinated development of driverless and smart transportation.

Research on the Impact of Information Sharing on the Stone Trade Supply Chain between China and South Korea

Yanlu Yang(Yanlu Yang),Yiyuan Wang(Yiyuan Wang),Minghao Gao(Minghao Gao) 동아시아무역학회 2022 Journal of East Asian Trade(JEAT) Vol.4 No.2

Purpose - Based on the important influence of information, this paper studies the impact of information sharing on the Stone trade supply chain achievements between China and Korea and the influencing factors of information sharing in the stone trade supply chain. Design/Methodology/Approach - Based on the important role of information sharing, this study explored the connotation of information sharing, the categories of influencing, factors and the types of supply chain achievements through theoretical traceability, and empirically analyzed the correlation between various variables through questionnaires and spss.12. Findings - This study believes that the influencing factors of information sharing include information sharing intention, transaction dependence, enterprise reputation, product characteristics, and information reading ability. The stone trade supply chain performances between China and South Korea include price, supply time, quality, flexibility, and stone resource conservation. In addition to product characteristics, other influence factors positively impact information sharing, which positively impacts supply chain performances. Research Implications - This study is of great significance to improve the information sharing level of China’s South Korea stone trade supply chain. It provides constructive suggestions for improving the information-sharing level.

Multi-level Feeder Queue Optimization Charging Model of Electric Vehicle and its Implementation of M-R Algorithm

Wang Wei,Ai Minghao,Chen Naishi,Ge Xianjun,Pu Tianjiao 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.3

Recently, issues of energy shortage and environment pollution of mankind society become more and more serious. Production of electric vehicles provides a new idea for mankind to solve this kind of issues. However, large-scale electric vehicles put into operation and connected to the grid is a major challenge to the security and stability of power grid. This paper references the job scheduling algorithm in computer operator system and presents a multi-level feeder queue optimization charging model with comprehensive consideration of the grid-side power load and charging fairness. According to this model we charge for the electric vehicles in regional grid, on the basis of ensuring fairness, realizing optimized charging, to ensure grid security and stability and improve the resource utilization rate. The implementation of multi-level feeder queue optimization charging model of electric vehicles in regional grid requires the fusion of power grid, cars networking, charging station networking and other information. With the development of the industry, the integration of multiple information sources will produce massive heterogeneous data, showed a trend of big data, and its storage and calculating will become a bottleneck. Hadoop open source cloud computing platform can set computing cluster to implement such a big data parallel processing. In this paper, I implement the model in the cloud computing platform through designing the model’s HBase distributed data storage and M-R parallel computing mode.

A Chemical Kinetic Model Including 54 Reactions for Modeling Air Nonequilibrium Inductively Coupled Plasmas

Minghao Yu,Wei Wang,Jiafeng Yao,Borui Zheng 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.10

The objective of the present study is the development of a comprehensive air chemical kinetic model that includes 11 species and 54 chemical reactions for the numerical investigation of air nonequilibrium inductively coupled plasmas. The two-dimensional, compressible Navier-Stokes equations coupled with the electromagnetic-field equations were employed to describe the fun- damental characteristics of an inductive plasma. Dunn-Kangs 32 chemical-reaction model of air was reconstructed and used as a comparative model. The effects of the different chemical kinetic models on the ow field were analyzed and discussed at identical/different working pressures. The results theoretically indicate that no matter the working pressure is low or high, the use of the 54 chemical kinetic model presented in this study is a better choice for the numerical simulation of a nonequilibrium air ICP.

Peridynamic simulation of brittle-ice crushed by a vertical structure

Minghao Liu,Qing Wang,Wei Lu 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.2

Sea ice is the main factor affecting the safety of the Arctic engineering. However, traditional numerical methods derived from classical continuum mechanics have difficulties in resolving discontinuous problems like ice damage. In this paper, a non-local, meshfree numerical method called “peridynamics”, which is based on integral form, was applied to simulate the interaction between level ice and a cylindrical, vertical, rigid structure at different velocities. Ice in the simulation was freshwater ice and simplified as elastic-brittle material with a linear elastic constitutive model and critical equivalent strain criterion for material failure in state-based peridynamics. The ice forces obtained from peridynamic simulation are in the same order as experimental data. Numerical visualization shows advantages of applying peridynamics on ice damage. To study the repetitive nature of ice force, damage zone lengths of crushing failure were computed and conclude that damage zone lengths are 0.15e0.2 times as ice thickness.

Risk Perception of Chinese Urban Residents: An Institutional Theory Perspective

Wenbin Wang,Donggen Rui,Minghao Huang 서울대학교 사회발전연구소 2021 Journal of Asian Sociology Vol.50 No.4

Peridynamic simulation of brittle-ice crushed by a vertical structure

Liu, Minghao,Wang, Qing,Lu, Wei The Society of Naval Architects of Korea 2017 International Journal of Naval Architecture and Oc Vol.9 No.2

Sea ice is the main factor affecting the safety of the Arctic engineering. However, traditional numerical methods derived from classical continuum mechanics have difficulties in resolving discontinuous problems like ice damage. In this paper, a non-local, meshfree numerical method called "peridynamics", which is based on integral form, was applied to simulate the interaction between level ice and a cylindrical, vertical, rigid structure at different velocities. Ice in the simulation was freshwater ice and simplified as elastic-brittle material with a linear elastic constitutive model and critical equivalent strain criterion for material failure in state-based peridynamics. The ice forces obtained from peridynamic simulation are in the same order as experimental data. Numerical visualization shows advantages of applying peridynamics on ice damage. To study the repetitive nature of ice force, damage zone lengths of crushing failure were computed and conclude that damage zone lengths are 0.15-0.2 times as ice thickness.

Effects of the nozzle throat diameter on the flow properties of an inductively coupled plasma heater

Yu Minghao,Wang Zhe 한국물리학회 2021 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.79 No.11

In this study, the non-equilibrium subsonic and supersonic inductively coupled plasmas (ICPs) in an ICP heater were numerically simulated and reproduced from the torch to the conical nozzle to the vacuum chamber. The flow field characteristics and the thermal non-equilibrium characteristics are obtained. Through an analysis and discussion of the plasma parameters with different nozzle throat diameters, the effects of the nozzle throat diameter on plasma properties such as the pressure, plasma velocity and temperature are obtained from the simulated results. The maximum velocity is found to appear at the exit of the nozzle, and the velocity of flow generated by a nozzle with a smaller throat diameter is found to be bigger. The pressure, velocity and temperature in the vacuum chamber fluctuate twice due to the shock wave at the nozzle’s exit. The position of the maximum temperature appears to be located in the center of the coil heating while the position of the high temperature core produced by a small diameter nozzle is closer to the inlet than that of the large diameter nozzle is. Due to the interaction between the shock wave produced by the nozzle and the plasma flow, the thermal plasma forms several independent hot heating cores in the vacuum chamber. The maximum values of the electron number density and the electric field intensity are bigger when the throat diameter of the nozzle is smaller.