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Amperometric Determination of Nitrite at Poly(Methylene Blue)-Modified Glassy Carbon Electrode
Xu, Guang-Ri,Xu, Guifang,Xu, Ming-Lu,Zhang, Zhengqing,Tian, Yuan,Choi, Han-Nim,Lee, Won-Yong Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.2
Electrochemical characteristics of nitrite ion were investigated at a poly(methylene blue)-modified glassy carbon electrode by cyclic voltammetry and differential pulse voltammetry. The poly(methylene blue)-modified glassy carbon electrode exhibited enhanced anodic signals for nitrite. The effects of key parameters on the detection of nitrite were evaluated at the modified electrode, such as pH, accumulation time, and scan rate. Under optimum condition, the chemically modified electrode can detect nitrite in the concentration range $2.0{\times}10^{-6}$ to $5.0{\times}10^{-4}$ M with the detection limit of $2.0{\times}10^{-6}$ M and a correlation coefficient of 0.999. The detection of nitrite using the chemically modified electrode was not affected by common ions such as $Na^+$, $K^+$, $Ca^{2+}$, $Cl^-$, $HPO_4^{2-}$ and $H_2PO_4^- $. The modified electrode showed good stability and reproducibility. The practical application of the present method was successfully applied to the determination of nitrite ion in cabbage samples.
Xu, Ming-Lu,Li, Gao,Moon, Dong-Cheol,Lee, Chong-Soon,Woo, Mi-Hee,Lee, Eung-Seok,Jahng, Yurng-Dong,Chang, Hyeun-Wook,Lee, Seung-Ho,Son, Jong-Keun The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.7
Four alkaloids (1-4), three quinolone alkaloids (5-7), and three flavanoid glucosides (8-10) were isolated from the fruits of Evodia officinalis Dode, and their structures were determined from chemical and spectral data. Compounds, 3, 8, 9 and 10 were isolated from this plant for the first time. Of these compounds, 1-3 and 5-7 exhibited moderate cytotoxicities against cultured human colon carcinoma (HT-29), human breast carcinoma (MCF-7), and human hepatoblastoma (HepG-2). Compound 8 showed strong inhibitory effects on DNA topoisomerases I and II (70 and 96% inhibition at a concentration of $20\;{\mu}M$, respectively).
A New Stilbene Glucoside from the Roots of Polygonum multiflorum Thunb.
Ming-Lu Xu,Ming Shan Zheng,Yeon-Kyong Lee,문동철,Chong-Soon Lee,우미희,정병선,Eung Seok Lee,장영동,장현욱,이승호,손종근 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.11
One new stilbene glucoside (6), along with five known compounds (1-5), were isolated from the roots of Polygonum multiflorum Thumb., and their chemical structures established based on physicochemical and spectroscopic data. Of the compounds, compound 3 showed DNA topoisomerase I and II inhibitory activities.
Ming Lu Xu,Lan Wang,Jian He Hu,Myeong-Hyeon Wang 한국식품영양과학회 2009 Preventive Nutrition and Food Science Vol.14 No.4
Three compounds, vanillic acid, p-hydroxylcinnamic acid, p-hydroxybenzaldehyde have been isolated from the ethylacetate extract of Sparganium stoloniferum Buch.-Ham roots using silica gel open column chromatography, preparative thin-layer chromatography (pTLC) and reverse phase high performance liquid chromatography. The structures of the compounds were established on the basis of IR, extensive 1D NMR, and MS analyses. The ethylacetate (EtOAc) extract, vanillic acid, and p-hydroxybenzaldehyde showed α-glucosidase inhibition activity of 72.71%, 20.13%, and 30.42%, at the concentration of 10 ㎍/mL, respectively. The EtOAc extract exhibited strong antioxidant activity with an IC50 value of 24.37 ㎍/mL against DPPH radical scavenging activity, the vanillic acid, p-hydroxylcinnamic acid, and p-hydroxybenzaldehyde with an IC50 value of 2.10 μM, 1.59 μM, and 2.72 μM against DPPH, respectively.
Xu, Ming Lu,Wang, Lan,Hu, Jian He,Wang, Myeong-Hyeon The Korean Society of Food Science and Nutrition 2009 Preventive Nutrition and Food Science Vol.14 No.4
Three compounds, vanillic acid, p-hydroxylcinnamic acid, p-hydroxybenzaldehyde have been isolated from the ethylacetate extract of Sparganium stoloniferum Buch.-Ham roots using silica gel open column chromatography, preparative thin-layer chromatography (pTLC) and reverse phase high performance liquid chromatography. The structures of the compounds were established on the basis of IR, extensive 1D NMR, and MS analyses. The ethylacetate (EtOAc) extract, vanillic acid, and p-hydroxybenzaldehyde showed $\alpha$-glucosidase inhibition activity of 72.71%, 20.13%, and 30.42%, at the concentration of 10 ${\mu}g/mL$, respectively. The EtOAc extract exhibited strong antioxidant activity with an $IC_50$ value of 24.37 ${\mu}g/mL$ against DPPH radical scavenging activity, the vanillic acid, p-hydroxylcinnamic acid, and p-hydroxybenzaldehyde with an $IC_50$ value of 2.10 ${\mu}M$, 1.59 ${\mu}M$, and 2.72 ${\mu}M$ against DPPH, respectively.
A New Stilbene Glucoside from the Roots of Polygonum multiflorum Thunb.
Xu, Ming-Lu,Zheng, Ming Shan,Lee, Yeon-Kyong,Moon, Dong-Cheol,Lee, Chong-Soon,Woo, Mi-Hee,Jeong, Byeong-Seon,Lee, Eung-Seok,Jahng, Yurng-Dong,Chang, Hyeun-Wook,Lee, Seung-Ho,Son, Jong-Keun The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.11
One new stilbene glucoside (6), along with five known compounds (1-5), were isolated from the roots of Polygonum multiflorum Thumb., and their chemical structures established based on physicochemical and spectroscopic data. Of the compounds, compound 3 showed DNA topoisomerase I and II inhibitory activities.
Association Between the Ku70-1310C/G Promoter Polymorphism and Cancer Risk: a Meta-analysis
Xu, Lu,Ju, Xiao-Bing,Li, Pu,Wang, Jue,Shi, Zhu-Mei,Zheng, Ming-Jie,Xue, Dan-Dan,Xu, Yan-Jie,Yin, Yong-Mei,Wang, Shui,You, Yong-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2
Ku70 plays an important role in DNA double-strand break repair. Studies revealing conflicting results on the role of the Ku70-1310C/G promoter polymorphism on cancer risk led us to perform a meta-analysis to investigate this relationship. Ten case-control studies with 2566 cases and 3058 controls were identified. Odds ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of associations. The overall results suggested no association between the Ku70-1310C/G promoter polymorphism and total cancer risk. However, on stratified analysis, significantly increased risks were observed among the Asian population (GG vs. CC: OR=1.50, 95%CI=1.10-2.06; GG vs. CC/CG: OR=1.47, 95%CI=1.07-2.01) and population-based case-control studies (GG vs. CC: OR=1.57, 95%CI=1.12-2.22; CG vs. CC: OR=1.35, 95%CI=1.11-1.64; CG/GG vs. CC: OR=1.37, 95%CI=1.14-1.65). Additionally, variant genotypes were associated with a significantly increased breast cancer risk (GG vs. CC: OR=1.80, 95%CI=1.26-2.56; GG vs. CC/CG: OR=1.40, 95%CI=1.01-1.95).
Xu, Lu-Wei,Qian, Ming,Jia, Rui-Peng,Xu, Zheng,Wu, Jian-Ping,Li, Wen-Cheng,Huang, Wen-Bin,Chen, Xing-Guo Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
The aim of this study was to investigate the expression and significance of microsomal prostaglandin synthase-1 (mPGES-1) and Beclin-1 in the development of prostate cancer (PCa). Immunohistochemistry was performed on paraffin-embedded sections with rabbit polyclonal against mPGES-1 and Beclin-1 in 40 PCa, 40 benign prostatic hyperplasia (BPH) and 10 normal prostate specimens for this purpose. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied for mRNA expression of mPGES-1 and Beclin-1, while MTT assays were used to ascertain the best working concentration of the mPGES-1 inhibitor (CAY10526). The effect of CAY10526 treatment on expression of Beclin-1 in DU-145 cells was studied using Western blot analysis. Localization of Beclin-1 and mPGES-1 was in endochylema. Significant differences in expression was noted among PCa, BPH and normal issues (P<0.05). Beclin-1 expression inversely correlated with mPGES-1 expression in PCa tissue (P<0.05). CAY10526 could significantly block mPGES-1 expression and the proliferation of DU-145 cells (P<0.05), while increasing Beclin-1 levels (P<0.05). Overexpression of mPGES-1 could decrease the autophagic PCa cell death. Inhibiting the expression of mPGES-1 may lead to DU-145 cell death and up-regulation of Beclin-1. The results suggest that inhibition of mPGES-1 may have therapeutic potential for PCa in the future.
Cytotoxic Constituents Isolated from the Fruit Bodies of Hypsizigus marmoreus
Ming-Lu Xu,Jae-Young Choi,정병선,Gao Li,Kap-Rang Lee,이종순,우미희,이응석,장영동,장현욱,이승호,손종근 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.1
The bioactivity-guided fractionation of chloroform extracts of the fruit bodies of Hypsizigus marmoreus led to our isolation of (22E,24R)-ergosta-7,22-diene-3β,5α,6β-triol (1), ergosterol-3-O- β-D-glucopyranoside (2), 5α,8α-epidioxyergosta-6,22-dien-3β-ol (3), hypsiziprenol A9 (4), hypsiziprenol AA8 (5), hypsiziprenol AA9 (6) and hypsiziprenol BA10 (7). Among these seven isolates, compound 2 was identified for the first time from this plant. All compounds (1-7) exhibited moderate cytotoxicity towards cultured human colon carcinoma (HT-29), human breast carcinoma (MCF-7) and human hepatoblastoma (HepG-2) cell lines.
Ming-Lu Xu,Lan Wang,Gui-Fang Xu,Jian-He Hu,Myeong-Hyeon Wang 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.6
The antioxidant and antiproliferation effects of fractions from methanol extract of Sparganiun stoloniferum were investigated in this study. The ethyl acetate fraction of the methanol extract showed significant antioxidant activity against α,?α-diphenyl-picrylhydrazyl (DPPH) and hydroxyl ( · OH) radical scavenging, and it showed greater reducing power than the n-hexane, dichloromethane, n-butanol and water fractions. Qualitative HPLC analysis was performed on the extractions. The high content of phenolics in ethyl acetate fractions were believed to be responsible for its antioxidant activity, which was the strongest in all five fractions. Moreover, the ethyl acetate fraction showed a dose-dependent inhibition of cell proliferation with an EC?? of 139.21 ± 0.54 μg · mL?¹ against the human colon cell, HT-29. The anti-proliferative effects of the ethyl acetate fraction were associated with apoptosis. Semi-quantitative RT-PCR analysis indicates that, after treatment with the ethyl acetate fraction, the expression of caspase-3, p53, and Bax in HT-29 cells was significantly up-regulated and c-myc expression was induced. These findings demonstrate that the ethyl acetate fraction of S. stoloniferum has potent antioxidant and antiproliferation effects and induces apoptosis. Thus, the extract of S. stoloniferum may be considered as a potential functional food resource and complementary medicinal supplement.