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Cheng Baixiang,Feng Fan,Shi Fan,Huang Jinmei,Zhang Songbai,Quan Yue,Tu Teng,Liu Yanli,Wang Junjun,Zhao Ying,Zhang Min 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4
BACKGROUND: Although newly formed constructs of feasible pressure-preadjusted bone marrow mesenchymal stem cells (BMSCs) and platelet-rich fibrin (PRF) showed biomechanical flexibility and superior capacity for cartilage regeneration, it is still not very clear how BMSCs and seed cells feel mechanical stimuli and convert them into biological signals, and the difference in signal transduction underlying mechanical and chemical cues is also unclear. METHODS: To determine whether mechanical stimulation (hydrostatic pressure) and chemical cues (platelet-rich fibrin, PRF) activate canonical or noncanonical Wnt signaling in BMSCs, BMSCs cocultured with PRF were subjected to hydrostatic pressure loading, and the activation of the Wnt signaling molecules and expression of cartilage-associated proteins and genes were determined by western blotting and polymerase chain reaction (PCR). Inhibitors of canonical or noncanonical Wnt signaling, XVX-939 or L690,330, were adopted to investigate the role of Wnt signaling molecules in mechanically promoted chondrogenic differentiation of BMSCs. RESULTS: Hydrostatic pressure of 120 kPa activated both Wnt/b-catenin signaling and Wnt/Ca2? signaling, with the the maximum promotion effect at 60 min. PRF exerted no synergistic effect on Wnt/b-catenin signaling activation. However, the growth factors released by PRF might reverse the promotion effects of pressure on Wnt/Ca2? signaling. Real-time PCR and Western blotting results showed that pressure could activate the expression of Col-II, Sox9, and aggrecan in BMSCs cocultured with PRF. Blocking experiment found a positive role of Wnt/b-catenin signaling, and a negative role of Wnt/ Ca2? signaling in chondrogenic differentiation of the BMSCs. Mutual inhibition exists between canonical and noncanonical Wnt signaling in BMSCs under pressure. CONCLUSION: Wnt signaling participates in the pressure-promoted chondrogenesis of the BMSCs co-cultured with PRF, with canonical and noncanonical pathways playing distinct roles during the process.
Ding, Ji Cheng,Zhang, Teng Fei,Mane, Rajaram S.,Kim, Kwang-Ho,Kang, Myung Chang,Zou, Chang Wei,Wang, Qi Min Elsevier 2018 Vacuum Vol.149 No.-
<P><B>Abstract</B></P> <P>In this paper, bipolar pulse reactive magnetron sputtering with ion source assisted deposition method has been utilized for the deposition of nanocrystalline alumina (Al<SUB>2</SUB>O<SUB>3</SUB>) films at the temperature of 300 °C onto silicon (111) wafers, and cemented carbide substrates. The influence of ion source power, i.e. 0, 1.0, 1.5 and 2.0 kW on the structure, morphology, compressive stress and mechanical properties of the Al<SUB>2</SUB>O<SUB>3</SUB> films were investigated. In absence of ion source power assistance, an amorphous Al<SUB>2</SUB>O<SUB>3</SUB> film was dominant at deposition temperature of 300 °C. With ion source assisted deposition, the crystalline γ-Al<SUB>2</SUB>O<SUB>3</SUB> films were obtained at the same conditions, suggesting an importance of ion source power in crystallinity development of metal oxide films obtained from magnetron sputtering deposition method. Images of surface morphology clearly demonstrated the difference in granular sizes of film surfaces prepared with and without ion source powers. With increasing ion source power from 1.0 to 2.0 kW, the micro-hardness and compressive stress of the films were increased from 7 GPa to 13 GPa and 0.3 GPa to 1.1 GPa, respectively. Results revealed that the reactive magnetron sputtering with the ion source assisted deposition was a simple and effective way to prepare nanocrystalline γ-Al<SUB>2</SUB>O<SUB>3</SUB> films at low temperature.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Al<SUB>2</SUB>O<SUB>3</SUB> films were synthesized by Ar ion source-assisted bipolar pulsed sputtering technique. </LI> <LI> Nanocrystalline Al<SUB>2</SUB>O<SUB>3</SUB> films were obtained at relatively low deposition temperature (300 °C) and low bias voltage conditions. </LI> <LI> The orientation and microstructure of the films can be adjusted by altering the ion source power. </LI> <LI> The synthesis approach of crystalline Al<SUB>2</SUB>O<SUB>3</SUB> films in this study is promising for large-scale industrial applications. </LI> </UL> </P>
Enhanced Corrosion Resistance of PVD-CrN Coatings by ALD Sealing Layers
Wan, Zhixin,Zhang, Teng Fei,Ding, Ji Cheng,Kim, Chang-Min,Park, So-Won,Yang, Yang,Kim, Kwang-Ho,Kwon, Se-Hun SPRINGER SCIENCE + BUSINESS MEDIA 2017 NANOSCALE RESEARCH LETTERS Vol.12 No.1
<P>Multilayered hard coatings with a CrN matrix and an Al<SUB>2</SUB>O<SUB>3</SUB>, TiO<SUB>2</SUB>, or nanolaminate-Al<SUB>2</SUB>O<SUB>3</SUB>/TiO<SUB>2</SUB> sealing layer were designed by a hybrid deposition process combined with physical vapor deposition (PVD) and atomic layer deposition (ALD). The strategy was to utilize ALD thin films as pinhole-free barriers to seal the intrinsic defects to protect the CrN matrix. The influences of the different sealing layers added in the coatings on the microstructure, surface roughness, and corrosion behaviors were investigated. The results indicated that the sealing layer added by ALD significantly decreased the average grain size and improved the corrosion resistance of the CrN coatings. The insertion of the nanolaminate-Al<SUB>2</SUB>O<SUB>3</SUB>/TiO<SUB>2</SUB> sealing layers resulted in a further increase in corrosion resistance, which was attributed to the synergistic effect of Al<SUB>2</SUB>O<SUB>3</SUB> and TiO<SUB>2</SUB>, both acting as excellent passivation barriers to the diffusion of corrosive substances.</P>
Chih-Hao Hsu,Min-Cheng Teng,Siao-Syun Ke 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.10
Infrastructure refers to an aggregation of numerous facilities that constitute the backbone of urban operations. The lifeline systems are usually interdependent; thus, any damage to one system can cause a chain reaction, triggering failures in related and connected systems, leading to a cascade of disasters. Taiwan is located in the Circum-Pacific seismic zone, where earthquakes occur frequently. Moderate-to-large earthquakes can adversely affect the lifeline systems, severely impairing most urban functions. Therefore, understanding the effects of earthquakes of this intensity on lifeline systems as well as how these effects contribute to disaster scenarios in urban areas is a critical requirement for the Taiwanese government. From the disaster management perspective, this study constructed a seismic impact chain module for lifeline systems on the basis of an analysis of the direct and indirect impacts of seismic activity. Furthermore, the factor of infrastructure impact was examined using the matrix method. Simulations of 6.6 magnitude earthquakes along the Shanchiao fault were used to assess the impact chain of the lifeline system and the effectiveness of a hospital disaster rescue service. In the simulation, the lifeline systems in the epicentral area were directly damaged, whereas critical infrastructure was indirectly affected. Therefore, the study results will assist the authorities in making intelligent decisions regarding lifeline infrastructure and resource management.