사과 추출물과 phloretin에 의한 항염증 활성

김근호(Geun-Ho Kim),이은주(Eun-Joo Lee),류승민(Seung-Min Ryu),손호용(Ho-Yong Sohn),김종식(Jong-Sik Kim) 한국생명과학회 2021 생명과학회지 Vol.31 No.2

본 연구에서는 풋사과의 열수추출물(GAHW)과 에탄올추출물(GAE), 그리고 애사과 열수추출물(UAHW)을 제조하고 LPS로 활성화된 RAW264.7세포주를 이용하여 이들의 항염증 활성을 연구하였다. 모든 추출물이 세포생존율에는 영향을 미치지 않고 nitric oxide (NO) 생산을 저해하였으며, iNOS의 발현도 감소시켰다. 반면, UAHW만이 COX-2의 발현을 저해하였다. 또한 모든 추출물이 모든 MAPKs의 인산화를 감소시켰다. 모든 추출물이 ROS의 생산을 농도의존적으로 감소시켰으며, GAHW와 GAE에 의해 HO-1의 발현이 증가됨을 확인하였다. 또한, 사과 flavonoid인 phloretin과 phloridzin의 항염증 활성을 연구하였다. Phloretin은 농도의존적으로 NO의 생산을 저해하였으나, phloridzin은 NO 생산에 아무 영향이 없었다. 또한, phloretin은 iNOS와 COX-2 단백질의 발현을 감소시킨 반면, phloridzin은 두 단백질 발현에 영향을 주지 못하였다. 따라서, 이러한 연구결과는 사과 추출물은 MAPK 경로와 HO-1 경로를 조절함으로써 항염증 활성을 가지며, phloretin이 사과의 항염증 활성을 담당하는 파이토케미칼의 하나가 될 수 있음을 제시한다. In the present study, we prepared hot water extracts of green apple (GAHW) and unripe apple (UAHW), and ethanol extract of green apple (GAE), and investigated their anti-inflammatory activities in LPS-activated RAW264.7 cells. All extracts dramatically suppressed nitric oxide (NO) production in a dose-dependent manner in LPS-stimulated RAW264.7 cells without affecting cell viability. In addition, all extracts decreased the expression of iNOS, whereas UAHW only reduced the expression of COX-2. All extracts suppressed the phosphorylation of MAPKs (p38, ERK, and JNK) indicating all extracts show their anti-inflammatory activities via regulating MAPK pathway. Furthermore, all extracts reduced the production of reactive oxygen species in a dose-dependent manner and they increased the expression of heme oxygenase-I (HO-I) whereas UAHW could not. We also investigated whether apple flavonoids phloretin and phloridzin can have their anti-inflammatory activities in same in vitro model. Phloretin dramatically decreased NO production in a dose dependent manner without affecting cell viability, whereas phloridzin have no effects. Phloretin also reduced the expression of iNOS as well as COX-2, whereas phloridzin could not. Overall, these results suggest that apple extracts have their anti-inflammatory activities via regulating MAPKs and HO-1 pathways, and apple flavonoid phloretin can be one of phytochemicals responsible for anti-inflammatory effect of apple.

Endoscopic submucosal tunnel dissection salvage technique for ulcerative early gastric cancer.

Choi, Hyuk Soon,Chun, Hoon Jai,Seo, Min Ho,Kim, Eun Sun,Keum, Bora,Seo, Yeon Seok,Jeen, Yoon Tae,Lee, Hong Sik,Um, Soon Ho,Kim, Chang Duck,Ryu, Ho Sang WJG Press 2014 WORLD JOURNAL OF GASTROENTEROLOGY Vol.20 No.27

<P>Endoscopic submucosal dissection is an effective treatment modality for early gastric cancer (EGC), though the submucosal fibrosis found in ulcerative EGC is an obstacle for successful treatment. This report presents two cases of ulcerative EGC in two males, 73- and 80-year-old, with severe fibrosis. As endoscopic ultrasonography suggested that the EGCs had invaded the submucosal layer, the endoscopic submucosal tunnel dissection salvage technique was utilized for complete resection of the lesions. Although surgical gastrectomy was originally scheduled, the two patients had severe coronary heart disease, and surgeries were refused because of the risks associated with their heart conditions. The endoscopic submucosal tunnel dissection salvage technique procedures described in these cases were performed under conscious sedation, and were completed within 30 min. The complete en bloc resection of EGC using endoscopic submucosal tunnel dissection salvage technique was possible with a free resection margin, and no other complications were noted during the procedure. This is the first known report concerning the use of the endoscopic submucosal tunnel dissection salvage technique salvage technique for treatment of ulcerative EGC. We demonstrate that endoscopic submucosal tunnel dissection salvage technique it is a feasible method showing several advantages over endoscopic submucosal dissection for cases of EGC with fibrosis. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.</P>

성인에서 기관내삽관을 위한 Pipecuronium 일회 정주 적정 용량

최영석,윤석민,임상호,이미경,박영철,류태간 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.33 No.3

Background : Studies in animals suggest that pipecuronium dose not induce hemodynamic chan-ges related to histamine release or to an effect on the autonomic nervous system. Therefore the effects of bolus administration of large doses of pipecuronium, up to 0.20 mg/kg, on the intubation condition, onset and duration of neuromuscular blockade, heart rate and blood pressure were studied during fentanyl- nitrous oxide anesthesia. Method : Forty adults were randomly assigned to receive a bolus injection of either 0.05, 0.10, 0.15, 0.20 mg/kg of pipecuronium. Neuromuscular blockade was measured using mechanomyographic activity of the adductor pollicis muscle after supramaximal stimulation of the ulnar nerve. Four subgroups of 10 patients received pipecuronium doses of 0.05, 0.10, 0.15 and 0.20 mg/kg, respectively, as an intubating dose. Results : The times of onset and clinical duration(mean sem) after each dose were as follows: 0.05 mg/kg, 2.98 0.42 and 41.5 2.42 min; 0.10 mg/kg, 1.54 0.06 and 82.9 7.48 min; 0.15 mg/kg, 1.41 0.14 and 124.8 13.1 min; 0.20 mg/kg, 1.12 0.05 and 187.1 12.8 min. The intubation condition, time of onset and duration after doses of 0.05 mg/kg were significantly different from values after the higer doses. The duration was increased with dose-increments. No dose-related changes in heart rate or blood pressure were observed. Conclusion : The authors conclude that dose of 0.10 mg/kg and over has good intubation condition clinically and large bolus dose of pipecuronium can be safely used with a significantly prolonged duration of action without hemodynamic change. (Korean J Anesthesiol 1997; 33: 453∼457)

韓國産 蓼(여뀌)科 植物에 관한 本草學的 硏究 (I)

柳泓善,宋昊埈,辛民敎,金裕鎭,金台晛 한국전통의학연구소 1998 한국전통의학지 Vol.8 No.2

For the purpose of developing Korean Herbalogy with the plants of Polygonaceae which grow wild and is planted in Korea, the these and writings on herbalogy, from literature of successive generations, have been thoroughly investigative and the results obtained were as follows: 1. There were totaled to 16 genera and 156 species in Korea and among them medicinal plants are 12 genera, 47 species, some 30% in total, but the number of species may be added because of the similiar plants. 2. According to the oriental names which can be used for medical purpose, the medicinal plants belonging to the Polygonaceae family were classified as Herba 21, Radix 12, Foilum 8, Lignum 5, Flos 4, Fructus 5, Ramulus 2 and Semen 1. Thus it was noticed that Herba was the main kind. 3. According to sum of 156 species in Polygonaceae family, they were classified into Persicaria genera 63, Polygonum genera 23, Rumex genera 22, Bistorta genera 14, Aconogonum genera 6, Fagopyrum genera 5, Bilderdykia genera 4, Amblygonum genera 3, Reynoutria genera 3, Rheum genera 3, Pleuropterus genera 2, Pleuropteropyrum genera 2, Tovara genera 2, Ampelygonum genera 1, Homalocladium genera 1 and Oxyria genera 1. Thus it was noticed that Persicaria genera was the main kind, some 40.3% in total. 4. According to nature and flavour of medicinal plants, they were classified into cold, cool; 23 each, warmth, heat; 11 each, balance 11 and unidentified 5. Thus it was noticed that cold, cool is the main in nature and flavour of medicinal plants. From this result, it was revealed that the plants for medical purpose in Polygonaceae was 30% of the whole, in which HERBA was mostly abundunt and toxic plants was slightly poisonous that it will be used for clinical treatments more easily. It is considered that many experiements and clinical approaches must be continued to use Polygonaceae plants widely.

유방암 환자에서 bcl-2 단백 발현의 예후 인자로서의 의의

류진우,박종권,정민,박동국,김정택,이두선,이찬영,전호진 단국대학교 1998 論文集 Vol.32 No.-

Breast cancer in women pose a problem, and it can be difficult at times to evaluate the possibility of the cancer recurrence in node negative early cancer. The reliable prognostic markers are needed in breast cancer management to help clinicians identify the patient with the high risk of recurrence and arrive at more rational decisions for proper treatment. In order to investigate the prognostic significance of bcl-2, an immunohis-tochemical staining was performed in 35 tissue sections from paraffin blocks of primary breast cancer. Bcl-2 positivity was 43%. No relation could be observed between bcl-2 expression and known prognostic factors(tumor size, lymph node metastasis state, histologic grade, and nuclear grade), There was strong relation between bcl-2 expression and estrogen receptor positivity (ER) (P〈0.05). No relation is found between bcl-2 expression and c - erbB - 2 expression. These results suggest that bcl-2 expression is associated with a favorable prognostic factor(ER) but it is of no value as independent prognostic factor to the clinician in identifying the patients with high risk of recurrence.

용존산소농도 예측을 위한 추계학적 다중 입력모형

류병로,한양수,두민호,조성근 大田産業大學校 1998 한밭대학교 논문집 Vol.15 No.1

In the most cases we analyze a single time series without explicitly using information contained in the related time series In many forecasting situations, other events will systematically influence the series to be forecasted(the dependent variables), and therefore, there is need to go beyond a univariate forecasting model. Thus, we must build a forecasting model that incorporates more than one time series and introduces explicitly the dynamic characteristics of the system. Such a model in called a multiple time series model or transfer function model. The purpose of this study is to develop the stochastic stream water quality model of Puyea station in Keum river system. The performance of the multiplicative ARIMA model and the multiple input-single output transfer function model were examined through comparisons between the historical and generated monthly dissolved oxygen series. The result reveal that the multiple input-single output model lead to teh improved accuracy.

광폭하구에서의 오염물질 확신이송 모형 (BAYQUAL) 개발

류병로,한양수,두민호,유병연 大田産業大學校 1998 한밭대학교 논문집 Vol.15 No.1

The objective of this research is to develop the water quality simulation model (BAYQUAL) that deal with the physical and chemical aspects of rate/behavior of pollutants in the bay BAYQUAL is a two dimensional, time-variable finite element water quality model. This modeling system was designed to provide the generality and flexibility necessary for analyzing a verity of water quality problems in a diverse set of water bodies. The algorithm is composed of a hydrodynamic module(BAYQUAL) which solves the equations of motion and continuity, a pollutant dispersion module which solves the dispersion-advection equation. The applicability and feasibility of the model are discussed by applications of the model to the Kwangyang bay of South Coastal Waters of Korea. Based on the field data, the BAYQUAL model was calibrated and verified. The results were in good agreement with measured value within relative error of 3% for COD. Numerical simulations of velocity components and tide amplitude were agreed closely with the actual data.

전류제어형 PWM컨버터를 이용한 동기발전기용 여자시스템에 관한 연구

장수진,류동균,서민성,김준호,원충연,이진국 성균관대학교 2003 학술회의지원논문목록집 Vol.2003 No.-

동기발전기 출력전압은 여자시스템의 계자전류 제어에 의해 일정하게 유지된다. 고주파 PWM 컨버터(전류제어모드 buck컨버더)형태의 여자시스템은 부하변동이 발생하였을 때 동기발전기의 계자전류를 제어하게 된다. 이 논문은 정상상태 및 과도상태에서의 안정화를 개선하기 위하여 여자시스템의 설계 및 실험에 대해 다루었다. 시뮬레이션 및 실험 결과는 제안된 여자시스템이 50kW 동기발전기의 DVR에 의해 응답시간이 개선되었음을 보여주었다. The output voltage of synchronous generator is regulated constantly by field current control in excitation system. High frequency PWM converter (current control mode buck conveter) type excitation system for synchronous generator is able to control exciter current when the load change happened. This paper deals with the design and evaluation of the excitation system for a synchronous generator to improve the steady state and transient stability. The simulation and experimental results show that the proposed excitation system is able to improve the response time by the DVR(digital voltage regulator) of 5O[kW] synchronous generator.

Comparison of Bradykinin- and Platelet-Derived Growth Factor-Induced Phosphoinositide Turnover in NIH 3T3 Cells

Lee, Kee-Ho,Ryu, Yong-Wun,Yoo, Young-Do,Bai, Dong-Hoon,Yu, Ju-Hyun,Kim, Chang-Min Korean Society for Biochemistry and Molecular Biol 1996 Journal of biochemistry and molecular biology Vol.29 No.6

Phosphoinositide turnover in response to platelet-derived growth factor, epidermal growth factor, and bradykinin was evaluated in NIH 3T3 cells. Platelet-derived growth factor and bradykinin induced a significant increase in incorporation of $^{32}P$ into phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), and phosphatidylinositol 4.5-bisphosphate ($PIP_2$) in serum-starved NIH 3T3 cells. However, epidermal growth factor increased incorporation of $^{32}P$ into these phosphoinositides by only a small amount. Stimulation with platelet-derived growth factor, not bradykinin, caused a rapid elevation of PI and PIP kinase activities that were maximally activated within 10 min. The maximal levels of their elevation in cells with plateletderived growth factor stimulation were 3.2-fold for PI kinase, and 2.1-fold for PIP kinase. Short term pretreatment of NIH 3T3 cells with phorbol 12-myristate 13-acetate, activator of protein kinase C. caused an approximately 60% decrease in platelet-derived growth factor-induced PI kinase activities, indicating the feedback regulation of phosphoinositide turnover by protein kinase C. These results suggest that although the enhancement of phosphoinositide turnover is a rapidly occurring response in platelet-derived growth factor- or bradykinin-stimulated NIH 3T3 cells, phosphoinositide kinases may be associated with initial signal transduction pathway relevant to platelet-derived growth factor but not to bradykinin.

Comparison of Bradykinin - and Platelet - Derived Growth Factor - Induced Phosphoinositide Turnover in NIH 3T3 Cells

Kim, Chang Min,BAI, DONG HOON,Yu, Ju Hyun,Lee, Kee Ho,Ryu, Yong Wun,Yoo, Young Do 생화학분자생물학회 1982 BMB Reports Vol.29 No.6

Phosphoinositide turnover in response to platelet-derived growth factor, epidermal growth factor, and bradykinin was evaluated in NIH 3T3 cells. Platelet-derived growth factor and bradykinin induced a significant increase in incorporation of ^(32)P into phosphatidylinositol (PI), phosphatidylinositol 4-monophosphate (PIP), and phosphatidylinositol 4,5-bisphosphate (PIP₂) in serum-starved NIH 3T3 cells. However, epidermal growth factor increased incorporation of BHP into these phosphoinositides by only a small amount. Stimulation with platelet-derived growth factor, not bradykinin, caused a rapid elevation of PI and PIP kinase activities that were maximally activated within 10 min. The maximal levels of their elevation in cells with plateletderived growth factor stimulation were 3.2-fold for PI kinase, and 2.1-fold for PIP kinase. Short term pretreatment of NIH 3T3 cells with phorbol 12-myristate 13-acetate, activator of protein kinase C, caused an approximately 60% decrease in platelet-derived growth factor-induced PI kinase activities, indicating the feedback regulation of phosphoinositide turnover by protein kinase C. These results suggest that although the enhancement of phosphoinositide turnover is a rapidly occurring response in platelet-derived growth factor- or bradykinin-stimulated NIH 3T3 cells, phosphoinositide kinases may be associated with initial signal transduction pathway relevant to platelet-derived growth factor but not to bradykinin.