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Lihua Wei,Miguel A. PEREZ-RODRIGUEZ,Mario A. RODRIGUEZ-PEREZ 한국곤충학회 2016 Entomological Research Vol.46 No.3
Baculoviruses can be genetically engineered to express foreign genes; thus, their lethal potency and host range can be improved to produce more virulent bioinsecticides. Polydnavirus (PDV) genes have insecticidal bioactivities and could enhance the pathogenicity of the baculoviruses to control insect pests. The CrV1 gene from Cotesia rubecula polydnavirus is responsible for depolymerization of actin cytoskeleton in hemocytes, disabling its spread on foreign object surfaces. In this study, we tested the efficacy of the recombinant baculovirus (AcMNPV‐CrV1) under p10 promoter against second instar P. rapae larvae. The expression of the CrV1 gene in P. rapae larvae was verified with reverse transcription–polymerase chain reaction (RT‐PCR). AcMNPV‐CrV1 showed a significantly lower median lethal concentration (LC50) and shorter median lethal time (LT50) as compared with the AcMNPV wild‐type virus. These results suggested that the expression of CrV1 protein could successfully improve the insecticidal toxicity of baculovirus.
Héctor F. Quintero,Luz A. Mejia,Miguel Diaz-Rodriguez 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.7
This paper develops a model for studying the effect of joint clearances on the end-effector's loci for planar parallel manipulators with 2 degree-of-freedom (DOF). The condition for developing end-effector loci equations with respect to the joint clearance relies on the fact that at least one point of contact is always presented in each joints. The motivation of this work lies in the fact that the proposed procedure allows the study of parallel manipulators that can be considered as design concepts for developing an x-y cutting table for a textile industry. Numerical simulations enable to compare three 2-DOF planar parallel manipulators architectures: i) 2-RRR, ii) 2-RPR, and iii) 2-RPR. A Monte Carlo simulation allows us to find the area of possible end-effector locations for a specific value of clearance. Also, the equations allow us to find the largest distance from the desired location to the one obtained considering clearance. Findings shed some lights on establishing which parallel manipulator is less affected to joint clearance.
Espinoza-Moreno C. A.,Rodriguez-Rodriguez M.,Pellegrini-Cervantes M. J.,Barrios-Durstewitz C. P.,Núñez-Jaquez R. E.,Peinado-Guevara H. J.,Chinchillas-Chinchillas M.,Fajardo-San-Miguel G. 한국탄소학회 2021 Carbon Letters Vol.31 No.4
The use of recycled materials, such as the fne recycled aggregate made from concrete waste and carbon fber (CF) product of industrial waste, for the manufacture of conductive recycled mortars (CRM), transforms the mortar base cement normally made with cement:sand in a sustainable multifunctional material, conferring satisfactory mechanical and electrical properties for non-structural uses. This action provides ecological benefts, reducing the use of natural fne aggregates from rivers and the amount of concrete waste deposited in landflls resulting from construction waste. In this investigation the efect of the addition of CF on electrical properties in hardened, wet and dry state, electric percolation in dry state and fuidity of the wet mixture of a cement based CRM was evaluated: fne recycled aggregate: graphite powder, CRM specimens with dimensions of 4×4×16 cm. were manufactured for 3, 7 and 28 days of age and sand/cement ratios=1.00, graphite/cement=1.00, water/cement=0.60 and CF=0.1, 0.3, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% compared to the weight of cement. The results demonstrated the efect of the addition of CF in CRM, reducing fuidity of the mixtures due to the opposition generated by its physical interaction of CF with recycled sand or recycled fne aggregate and graphite powder (GP), in its case, placing the electric percolation percolation at 0.30% and 0.45% of CF for CRM with and without GP, respectively. Increases in electrical conductivity (EC) without the presence of GP are defned by the contact between the CF and the conductive paths formed. In contrast, with the presence of GP, the EC is defned by the contact between the CF and the GP simultaneously, forming conductive routes with greater performance in its EC.
Prolonged intensive care: muscular functional, and nutritional insights from the COVID-19 pandemic
Miguel Ángel Martínez-Camacho,Robert Alexander Jones-Baro,Alberto Gómez-González,Dalia Sahian Lugo-García,Pía Carolina Gallardo Astorga,Andrea Melo-Villalobos,Bárbara Kassandra Gonzalez-Rodriguez,Ánge 대한중환자의학회 2024 Acute and Critical Care Vol.39 No.1
During the coronavirus disease 2019 (COVID-19) pandemic, clinical staff learned how to manage patients enduring extended stays in an intensive care unit (ICU). COVID-19 patients requiring critical care in an ICU face a high risk of experiencing prolonged intensive care (PIC). The use of invasive mechanical ventilation in individuals with severe acute respiratory distress syndrome can cause numerous complications that influence both short-term and long-term morbidity and mortality. Those risks underscore the importance of proactively addressing functional complications. Mitigating secondary complications unrelated to the primary pathology of admission is imperative in minimizing the risk of PIC. Therefore, incorporating strategies to do that into daily ICU practice for both COVID-19 patients and those critically ill from other conditions is significantly important.
Ignacio Iglesias-Garriz,David Alonso,Carmen Garrote,Victoria Casares,Javier Vara,Jose Maria De la Torre,Miguel A. Rodriguez,Felipe Fernandez-Vazquez 한국심초음파학회 2020 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.28 No.1
BACKGROUND: Proper scaling of cardiac dimensions is of paramount importance in making correct decisions in clinical cardiology. The usual normalization of cardiac dimensions to overall body size assumes an isometric relationship. We sought to investigate these relationships to obtain the best allometric coefficient (AC) for scaling. METHODS: Ninety-seven healthy volunteers were included. The dimensions to be scaled were the left atrial volume, the end-diastolic and end-systolic left ventricular volumes, and the diameter of the tricuspid annulus. A Bayesian statistical analysis was applied with isometric coefficients as priors. RESULTS: The linear correlations between cardiac dimensions and body size were modest, ranging from 0.12 (-0.10–0.32) for the left atrial volume and height to 0.70 (0.58–0.80) for the end-diastolic volume and height. The ACs varied across the different cardiac dimensions and body size measurements. For the best linear relationships, the isometric coefficients were outside the 95% highest density interval of the posterior distribution for the left atrial volume-weight (AC: 0.7; 0.4–0.9) and end-diastolic volume-height (AC: 2.3; 1.7–2.9), whereas they were different from 1 for the left atrial volume-weight, end-diastolic volume, and diameter of the tricuspid annulus-body surface area (AC: 0.6; 0.3–0.8). Not scaling the cardiac dimensions to their corresponding ACs can lead to important errors in size estimations of cardiac structure. CONCLUSIONS: The ACs found in this study are somewhat different from the corresponding isometric coefficients and often different from 1. This finding should be considered when normalizing cardiac structures to body size when making clinical decisions.