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RISS 인기검색어
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- 저자 : Saeed Heidari Keshel (검색결과 4 건)
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Azadeh Izadyari Aghmiuni,Saeed Heidari Keshel,Mostafa Rezaei-Tavirani,Farshid Sefat,Arash Khojasteh,Masoud Soleimani,Farzad Pakdel 한국섬유공학회 2022 Fibers and polymers Vol.23 No.12
Tissue engineering provides new approaches to improve skin lesions. However, cell differentiation onto theengineered substrate with the skin-like pattern is the main challenge. Here we have tried to fabricate such the substrate viastudying the change in polymers ratios and molecular weight, and grafting scaffold with silk fibroin (SF) biomaterial. To thisend, chitosan and PEG were mixed at the volume ratios of 25:75, 50:50, and 65:35, and samples were lyophilized by thefreeze-drying method. Based on the result, the ratio of 65:35 indicated better physicomechanical properties than two otherscaffolds. Afterward, Chi/PEG scaffolds were prepared via mixing chitosan/PEG with (65:35) and PEG molecular weights of2000, 4000, 6000, 10000 Da. It was found that the increase of PEG molecular weight (>4000) was led to the reduction intensile strength and elongation of the scaffold network. Hence, PEG4000 was selected as the optimum molecular weight todesign SF-grafted Chi/PEG scaffold. Therefore, Chi/PEG4000-SF scaffold was designed to evaluate the volume ratio of SF(1 %, 3 %, 5 %) and compare data with the decellularized dermis. The results showed Chi/PEG4000-SF(3%) scaffold not onlywas led to the same elongation as Chi/PEG-SF(5%) scaffold but also created the dermis-like modulus. Moreover, Chi/PEGSF(3%) provided higher expression level of keratinocytes (bio-mimetic pattern) than decellularized dermis due to betterphysicomechanical properties. Hence, it seems that engineered scaffolds can be a more suitable option than native tissue (dueto removal of limitations such as donor sites and immunogenicity, and their mechanical properties). This study can providenovel insight into the better design of skin-engineered scaffolds.
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Hasan Tahermansouri,Atieh Mirosanloo,Saeed Heidari Keshel,Mossa Gardaneh 한국탄소학회 2016 Carbon Letters Vol.17 No.-
The attachment of 2-aminobenzamide to carboxylated multi-wall carbon nanotubes (MWCNTs)- COOH was achieved through the formation of amide bonds. Then, the functionalized MWCNTs, MWCNT-amide, were treated by phosphoryl chloride to produce MWCNT-quin. The products were characterized by Fourier transform infrared spectroscopy, Raman spectroscopy, scanning electron microscopy, thermogravimetric analysis, derivative thermogravimetric, steady-state fluorescence spectroscopy, and solubility testing. MWCNT-quin showed photo-electronic properties, which is due to the attachment of the 4-hydroxyquinazoline groups to them as proved by steady-state fluorescence spectroscopy. This suggests intramolecular interactions between the tubes and the attached 4-hydroxyquinazoline. The toxicity of the samples was evaluated in human embryonic kidney HEK293 and human breast cancer SKBR3 cell lines, and the viable cell numbers were measured by 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) after the cells were cultured for 24 h. Cellular investigations showed that the modified MWCNTs, particularly MWCNT- quin, have considerably significant toxic impact on SKBR3 as compared to HEK293 at the concentration of 5 μg/mL.
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Cross-Linked Collagen Scaffold from Fish Skin as an Ideal Biopolymer for Tissue Engineering
Esmaeil Biazar,Mahshad Kamalvand,Saeed Heidari Keshel,Bahareh Pourjabbar,Mustafa Rezaei-Tavirani 한국재료학회 2022 한국재료학회지 Vol.32 No.4
Collagen is one of the most widely used biological materials in medical design. Collagen extracted from marine organisms can be a good biomaterial for tissue engineering applications due to its suitable properties. In this study, collagen is extracted from fish skin of Ctenopharyngodon Idella; then, the freeze drying method is used to design a porous scaffold. The scaffolds are modified with the chemical crosslinker N-(3-Dimethylaminopropyl)-N'-ethyl carbodiimide hydrochloride (EDC) to improve some of the overall properties. The extracted collagen samples are evaluated by various analyzes including cytotoxicity test, SDS-PAGE, FTIR, DSC, SEM, biodegradability and cell culture. The results of the SDS-PAGE study demonstrate well the protein patterns of the extracted collagen. The results show that cross-linking of collagen scaffold increases denaturation temperature and degradation time. The results of cytotoxicity show that the modified scaffolds have no toxicity. The cell adhesion study also shows that epithelial cells adhere well to the scaffold. Therefore, this method of chemical modification of collagen scaffold can improve the physical and biological properties. Overall, the modified collagen scaffold can be a promising candidate for tissue engineering applications.
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Azadeh Izadyari Aghmiuni,Mazyar Sharifzadeh Baei,Saeed Heidari Keshel,Azim Akbarzadeh Khiyavi 한국섬유공학회 2020 Fibers and polymers Vol.21 No.1
Dermal lesions and chronic wounds associated with burns or some diseases like diabetes are the more importantpublic health concerns which can affect the quality of life. Currently, tissue engineering is considered as the most effectivetherapeutic method although the design of polymeric substrates for epidermal-dermal differentiation and wound healing(scar-free) is the main challenge. For this purpose, we designed a hybrid three-dimensional scaffold (CPCP) based oncollagen/chitosan modified by PEG/PCL composite that can imitate differentiation pattern of both epidermis/dermis cells, viamimicking the structure and function of human skin. The physicochemical, mechanical and biological properties of designedscaffolds were evaluated to study their function for skin tissue engineering applications. Comparison of FTIR analysisshowed a chemical similarity between CPCP and decellularized dermal matrix (DDM). Our results showed that combinationof two natural/two synthetic polymers led to the formation of stronger 3D-network together with higher modulus (~18), waterabsorption (4-fold), porosity (~92) and consequently lower pores size (~54 μm), compared to natural, synthetic and natural/synthetic copolymer-based scaffolds. The observation of human skin fibroblast cells proliferation and morphology showedthat CPCP was more beneficial to cell adhesion, proliferation, and extension than that of other designed scaffolds due to itshydrophilicity and higher wettability (WCA=60 o). According to the results of RT-PCR, the more expression of epidermaldermalkeratinocytes induced by human-adipose-derived stem cells was observed on the CPCP along with a pattern similar toskin. The results demonstrate CPCP can act as a super-absorbent substrate/dressing for continuous absorption of woundexudates. Furthermore, it can potentially be effective for re-epithelialization of skin together with its derivative (hair follicles,sebaceous/sweat glands). This study indicates new insights into the design of skin- engineered scaffolds.
ScienceON
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