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RISS 인기검색어
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- 저자 : Maria Piccione (검색결과 3 건)
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Echocardiography and electrocardiography as means to evaluate potential performance in horses
Giuseppe Piccione,Carlos Lightowler,Elisabetta Giudice,Gerardo Romei del Olmo,Maria Laura Cattaneo 대한수의학회 2004 Journal of Veterinary Science Vol.5 No.3
Prediction of potential performance is one of the goals of exercise physiology investigations. When Selecting a horse for competition, one of the main objectives is to choose the one that predictably will reveal a competitive aptitude above the average. The horses used in this study underwent a two-dimensional echocardiography study and a conventional 3 leads electrocardiogram. The results show that heart score is not an appropriate index to evaluate the heart size in the horse. On the other hand, there are currently more suitable and accurate procedures such as echocardiography that allow performing a clear anatomical evaluation and accurate measurement in order to calculate LVMM and to predict performance.
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Growth and Differentiation of Circulating Stem Cells After Extensive Ex Vivo Expansion
Barbon Silvia,Rajendran Senthilkumar,Bertalot Thomas,Piccione Monica,Gasparella Marco,Parnigotto Pier Paolo,Di Liddo Rosa,Conconi Maria Teresa 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.3
Background: Stem cell therapy is gaining momentum as an effective treatment strategy for degenerative diseases. Adult stem cells isolated from various sources (i.e., cord blood, bone marrow, adipose tissue) are being considered as a realistic option due to their well-documented therapeutic potentials. Our previous studies standardized a method to isolate circulating multipotent cells (CMCs) that are able to sustain long term in vitro culture and differentiate towards mesodermal lineages. Methods: In this work, long-term cultures of CMCs were stimulated to study in vitro neuronal and myogenic differentiation. After induction, cells were analysed at different time points. Morphological studies were performed by scanning electron microscopy and specific neuronal and myogenic marker expression were evaluated using RT-PCR, flow cytometry and western blot. For myogenic plasticity study, CMCs were transplanted into in vivo model of chemically-induced muscle damage. Results: After neurogenic induction, CMCs showed characteristic dendrite-like morphology and expressed specific neuronal markers both at mRNA and protein level. The calcium flux activity of CMCs under stimulation with potassium chloride and the secretion of noradrenalin confirmed their ability to acquire a functional phenotype. In parallel, the myogenic potential of CMCs was confirmed by their ability to form syncytium-like structures in vitro and express myogenic markers both at early and late phases of differentiation. Interestingly, in a rat model of bupivacaine-induced muscle damage, CMCs integrated within the host tissue taking part in tissue repair. Conclusion: Overall, collected data demonstrated long-term cultured CMCs retain proliferative and differentiative potentials suggesting to be a good candidate for cell therapy. Background: Stem cell therapy is gaining momentum as an effective treatment strategy for degenerative diseases. Adult stem cells isolated from various sources (i.e., cord blood, bone marrow, adipose tissue) are being considered as a realistic option due to their well-documented therapeutic potentials. Our previous studies standardized a method to isolate circulating multipotent cells (CMCs) that are able to sustain long term in vitro culture and differentiate towards mesodermal lineages. Methods: In this work, long-term cultures of CMCs were stimulated to study in vitro neuronal and myogenic differentiation. After induction, cells were analysed at different time points. Morphological studies were performed by scanning electron microscopy and specific neuronal and myogenic marker expression were evaluated using RT-PCR, flow cytometry and western blot. For myogenic plasticity study, CMCs were transplanted into in vivo model of chemically-induced muscle damage. Results: After neurogenic induction, CMCs showed characteristic dendrite-like morphology and expressed specific neuronal markers both at mRNA and protein level. The calcium flux activity of CMCs under stimulation with potassium chloride and the secretion of noradrenalin confirmed their ability to acquire a functional phenotype. In parallel, the myogenic potential of CMCs was confirmed by their ability to form syncytium-like structures in vitro and express myogenic markers both at early and late phases of differentiation. Interestingly, in a rat model of bupivacaine-induced muscle damage, CMCs integrated within the host tissue taking part in tissue repair. Conclusion: Overall, collected data demonstrated long-term cultured CMCs retain proliferative and differentiative potentials suggesting to be a good candidate for cell therapy.
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Identification of novel mutations in L1CAM gene by a DHPLC-based assay
Mirella Vinci,Michele Falco,Lucia Castiglia,Lucia Grillo,Angela Spalletta,Maurizio Sturnio,Ornella Galesi,Michele Salemi,Angelo Gloria,Silvestra Amata,Maria Piccione,Vincenzo Antona,Girolamo Aurelio V 한국유전학회 2016 Genes & Genomics Vol.38 No.12
X-linked hydrocephalus, MASA syndrome, X-linked complicated Spastic Paraplegia Type I, and X-linked partial agenesis of the corpus callosum are rare diseases mainly affecting male population and broadly referred as L1 syndrome, caused by mutations in the L1CAM gene. In the present study 36 boys and a male fetus whose clinical features were consistent with L1 syndrome were analyzed by dHPLC assay and direct sequencing of L1CAM gene. Sequence analysis of the 14 different aberrant dHPLC elution profiles demonstrated that six of them were associated with already reported polymorphisms, four with previously described causative variants while the remaining four represented novel L1CAM mutations. The dHPLC method proposed identified eight (21 %) causative L1CAM mutations in our patients while direct sequencing failed to detect any variation in patients negative to dHPLC analysis. We conclude that the dHPLC assay represents a fast and efficient method for the screening of L1CAM mutations and that L1 syndrome should be considered in the differential diagnosis of intellectual disability in children, especially when other signs such as hydrocephalus or adducted thumbs are present.
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