Genome‑wide characterization of simple sequence repeats in Palmae genomes

Manee M. Manee,Badr M. Al‑Shomrani,Mohamed B. Al‑Fageeh 한국유전학회 2020 Genes & Genomics Vol.42 No.5

Background Microsatellites or simple sequence repeats (SSRs) have become the most significant DNA marker technology used in genetic research. The availability of complete draft genomes for a number of Palmae species has made it possible to perform genome-wide analysis of SSRs in these species. Palm trees are tropical and subtropical plants with agricultural and economic importance due to the nutritional value of their fruit cultivars. Objective This is the first comprehensive study examining and comparing microsatellites in completely-sequenced draft genomes of Palmae species. Methods We identified and compared perfect SSRs with 1–6 bp nucleotide motifs to characterize microsatellites in Palmae species using PERF v0.2.5. We analyzed their relative abundance, relative density, and GC content in five palm species: Phoenix dactylifera, Cocos nucifera, Calamus simplicifolius, Elaeis oleifera, and Elaeis guineensis. Results A total of 118241, 328189, 450753, 176608, and 70694 SSRs were identified, respectively. The six repeat types were not evenly distributed across the five genomes. Mono- and dinucleotide SSRs were the most abundant, and GC content was highest in tri- and hexanucleotide SSRs. Conclusion We envisage that this analysis would further substantiate more in-depth computational, biochemical, and molecular studies on the roles SSRs may play in the genome organization of the palm species. The current study contributes a detailed characterization of simple sequence repeats in palm genomes.

A Northeast Asian Multilateral Security Cooperation Regime : Retrospects and Prospects

Mane, Heo 부산대학교 과학교육연구소 2003 교사교육연구 Vol.42 No.-

Superior selectivity and enhanced response characteristics of palladium sensitized vanadium pentoxide nanorods for detection of nitrogen dioxide gas

Mane, A.A.,Suryawanshi, M.P.,Kim, J.H.,Moholkar, A.V. Academic Press 2017 Journal of Colloid and Interface Science Vol. No.

<P><B>Abstract</B></P> <P>Vanadium pentoxide (V<SUB>2</SUB>O<SUB>5</SUB>) nanorods have been deposited onto the glass substrates by spraying 75ml of 30mM vanadium trichloride (VCl<SUB>3</SUB>) solution at optimized substrate temperature of 400°C. The XRD study confirms the formation of orthorhombic crystal structure of V<SUB>2</SUB>O<SUB>5</SUB> nanorods. The FE-SEM micrograph shows the nanorods-like morphology of V<SUB>2</SUB>O<SUB>5</SUB>. The presence of palladium (Pd) in the Pd-sensitized V<SUB>2</SUB>O<SUB>5</SUB> nanorods is confirmed using EDAX study. The gas sensing measurements show that the Pd-sensitized V<SUB>2</SUB>O<SUB>5</SUB> sensing material is an outstanding candidate for nitrogen dioxide (NO<SUB>2</SUB>) gas detection. Obtained results demonstrate that the Pd-sensitized V<SUB>2</SUB>O<SUB>5</SUB> nanorods show the superior selectivity for NO<SUB>2</SUB> gas in comparison with other gases such as NH<SUB>3</SUB>, H<SUB>2</SUB>S, CO, CO<SUB>2</SUB> and SO<SUB>2</SUB> at an operating temperature of 200°C. It shows the 75% response for 100ppm NO<SUB>2</SUB> gas concentration with response and recovery times of 22s and 126s, respectively. Finally, the gas sensing mechanism based on chemisorption process is proposed to illustrate how Pd nanoparticles affect the gas sensing characteristics (response and response-recovery times).</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Fast response of sprayed vanadium pentoxide (V₂O₅) nanorods towards nitrogen dioxide (NO₂) gas detection

Mane, A.A.,Suryawanshi, M.P.,Kim, J.H.,Moholkar, A.V. Elsevier BV * North-Holland 2017 Applied Surface Science Vol.403 No.-

<P><B>Abstract</B></P> <P>The V<SUB>2</SUB>O<SUB>5</SUB> nanorods have been successfully spray deposited at optimized substrate temperature of 400°C onto the glass substrates using vanadium trichloride (VCl<SUB>3</SUB>) solution of different concentrations. The effect of solution concentration on the physicochemical and NO<SUB>2</SUB> gas sensing properties of sprayed V<SUB>2</SUB>O<SUB>5</SUB> nanorods is studied at different operating temperatures and gas concentrations. The XRD study reveals the formation of V<SUB>2</SUB>O<SUB>5</SUB> having an orthorhombic symmetry. The FE-SEM micrographs show the nanorods-like morphology of V<SUB>2</SUB>O<SUB>5</SUB>. The AFM micrographs exhibit a well covered granular surface topography. For direct allowed transition, the band gap energy values are found to be decreased from 2.45eV to 2.42eV. The nanorods deposited with 30mM solution concentration shows the maximum response of 24.2% for 100ppm NO<SUB>2</SUB> gas concentration at an operating temperature of 200°C with response and recovery times of 13s and 140s, respectively. Finally, the chemisorption mechanism of NO<SUB>2</SUB> gas on the V<SUB>2</SUB>O<SUB>5</SUB> nanorods is discussed.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Effect of solution concentration on physicochemical properties of sprayed V<SUB>2</SUB>O<SUB>5</SUB> nanorods is studied. </LI> <LI> Good re­sponse and short re­sponse- re­covery times of V<SUB>2</SUB>O<SUB>5</SUB> nanorods towards NO<SUB>2</SUB> gas show it is potential material for fab­ri­ca­tion of NO<SUB>2</SUB> sensor. </LI> <LI> The chemisorption mechanism of NO<SUB>2</SUB> gas on the V<SUB>2</SUB>O<SUB>5</SUB> nanorods is discussed. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Dye-sensitized solar cell and electrochemical supercapacitor applications of electrochemically deposited hydrophilic and nanocrystalline tin oxide film electrodes

Mane, R.S.,Chang, J.,Ham, D.,Pawar, B.N.,Ganesh, T.,Cho, B.W.,Lee, J.K.,Han, S.H. Elsevier 2009 Current Applied Physics Vol.9 No.1

The present article demonstrates the use of low-cost electrochemically synthesized hydrophilic and nanocrystalline tin oxide film electrodes at room temperature in dye-sensitized solar cells and electrochemical supercapacitors. A mixed phase of tin instead of single phase composed of uniformly distributed irregular spherical grains in a nanometer regime scale was obtained. Tin oxide film electrode showed efficient photoperformance when subjected to dye-sensitized solar cells. The interfacial and specific capacitances of 118.4μF/cm<SUP>2</SUP> and 43.07F/g, respectively, in 0.1M NaOH electrolyte were confirmed from cyclic-voltammetry measurement.

Electrochromism in indium-tin-oxide films for laser-writing application

Mane, R.S.,Cai, G.,Hoeil, C.,Lokhande, C.D.,Han, S.-H. North-Holland 2009 Physica E, Low-dimensional systems & nanostructure Vol.41 No.7

We report for the first time laser-writing application on an electrochemically NH<SUB>4</SUB><SUP>+</SUP> ion intercalated indium-tin oxide (ITO). NH<SUB>4</SUB><SUP>+</SUP> ion intercalation results into a color change from transparent to brown with a significant enhancement of the surface roughness. Both bare and intercalated ITO's are explored for their structural, surface morphological, and wettability studies. Upon NH<SUB>4</SUB><SUP>+</SUP> intercalation, both an optical absorbance and the surface wettability of ITO changes substantially. A micrometer-scale laser-writing application on intercalated ITO film is achieved after exposing an Ar-ion laser that is non-erasable even after a period of six months. This idea can be used for maintaining long-term safe secrete data.

Visions for the EU’s Federal Foreign Policy: For A Better Future

( Mane Heo ) 한국EU학회 2022 Asia-Pacific Journal of EU Studies Vol.20 No.1

The paper aims to explore a possibility of applying a federal foreign policy in the long tradition of the European integration. It tries to seek a more coordinated common foreign, security and defense policies in its efforts to ensure both more effectiveness and sustainment of CFSP and ESDP in a federal architecture, while it purports to develop European characters in the three fields, with the effectuation of a new Council for Federal Foreign Policy. The EU in the Post-Cold War period faces more uncertainties and challenges both internally and externally. It suggests the compelling necessities to apply federal foreign policy as part of institutional innovations that could move to an ever closer union and that could enhance European characters in the three fields.

Peak mooring forces in the horizontal interlaced multi-layered moored floating pipe breakwater

Mane, Vishwanath,Rajappa, Sacchi,Rao, Subba,Vittal, Hegde A. The Society of Naval Architects of Korea 2011 International Journal of Naval Architecture and Oc Vol.3 No.2

Present study aims to investigate the influence of relative breakwater width W/L (W=width of breakwater, L=wavelength), wave steepness $H_i/gT^2$ (Hi=incident wave height, T=wave period) and relative wave height d/W (d=water depth) on forces in the moorings of horizontal interlaced multi-layered moored floating pipe breakwater (HIMMFPB) model. Studies were conducted on scaled down physical models having three layers of Poly Vinyl Chloride (PVC) pipes, wave steepness $H_i/gT^2$ varying from 0.063 to 0.849, relative width W/L varying from 0.4 to 2.65 and relative spacing S/D=2 (S=horizontal centre-to-centre spacing of pipes, D=diameter of pipes). Peak mooring forces were also measured and data collected is analyzed by plotting non-dimensional graphs depicting variation of $f_s/{\gamma}W^2$ ($f_s$=Sea side Mooring force, ${\gamma}$=specific weight of water) & $f_l/{\gamma}W^2$ ($f_l$=Lee side Mooring force) with $H_i/gT^2$ for d/W varying from 0.082 to 0.276 and also variation of $f_s/{\gamma}W^2$ and $f_l/{\gamma}W^2$ with W/L for $H_i$/d varying from 0.06 to 0.400.

Potential of different salt forming agents on the formation of chitosan nanoparticles as carriers for protein drug delivery systems

Manee Luangtana‑anan,Jurairat Nunthanid,Sontaya Limmatvapirat 한국약제학회 2019 Journal of Pharmaceutical Investigation Vol.49 No.1

The effects of salt forming agents for chitosan on the potential for nanoparticle formation was investigated. The salt forms were prepared from the amino acid group, including glutamic and aspartic acids, and the alpha hydroxyl acid group, including lactic and glycolic acids. All types of chitosan salt could be used to prepare bovine serum albumin (BSA) loaded nanoparticles. The chitosan salts prepared from the amino acid group showed a higher salt formation ability as demonstrated using FTIR, hence a higher %encapsulation efficiency (%EE) and a reduction in zeta potential were obtained. The difference was due to the different organic acids used giving different polymer conformations and pH values in solution. Chitosan glutamate gave the highest salt formation ability and hence the highest %EE was obtained. The release of protein from all types of chitosan was similar and chitosan glutamate exhibited the highest release. Chitosan salt is therefore a material of choice for protein-loaded nanoparticles and the characteristics of nanoparticles can be readily modulated by different types of salt form.

L-carnitine Supplemented Extender Improves Cryopreserved-thawed Cat Epididymal Sperm Motility

Manee-In, S.,Parmornsupornvichit, S.,Kraiprayoon, S.,Tharasanit, T.,Chanapiwat, P.,Kaeoket, K. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.6

Cryopreservation of epididymal sperm is an effective technique to preserve genetic materials of domestic cats and wild felids when they unexpectedly die. However, this technique inevitably causes detrimental changes of cryopreserved-thawed spermatozoa, for example, by physical damage and excessive oxidative stress. L-carnitine is an antioxidant that has been used to improve sperm motility in humans and domestic animals. This study aimed to investigate the effects of L-carnitine on cat epididymal sperm quality following cryopreservation and thawing. After routine castration, cauda epididymides were collected from 60 cat testes. The epididymal spermatozoa from 3 cauda epididymides were pooled as 1 replicate. Spermatozoa samples (16 replicates) were examined for spermatozoa quality and then randomly divided into 4 groups: 0 mM L-carnitine (control), 12.5 mM, 25 mM and 50 mM L-carnitine. The sperm aliquots were then equilibrated and conventionally frozen. After thawing, sperm motility, plasma membrane integrity, DNA integrity and acrosome integrity were evaluated. The 25 mM L-carnitine significantly improved sperm motility compared with a control group (p<0.05), although this was not significantly different among other concentrations. In conclusion, supplementation of 25 mM L-carnitine in freezing extender improves cauda epididymal spermatozoa motility. The effects of L-carnitine on the levels of oxidative stress during freezing and thawing remains to be examined.