The Association between Whole Blood Viscosity and Coronary Collateral Circulation in Patients with Chronic Total Occlusion

Mehmet Serkan Cetin,Elif Hande Ozcan Cetin,Kevser Gülcihan Balcı,Selahattin Aydin,Emek Ediboglu,Muhammed Fatih Bayraktar,Mustafa Mücahit Balcı,Orhan Maden,Ahmet Temizhan 대한심장학회 2016 Korean Circulation Journal Vol.46 No.6

Background and Objectives: Coronary collateral circulation (CCC) has been attributed as inborn bypass mechanisms supporting ischemic myocardium. Various factors have been postulated in CCC. Whole blood viscosity (WBV) has been an underappreciated entity despite close relationships between multiple cardiovascular diseases. WBV can be calculated with a validated equation from hematocrit and total plasma protein levels for a low and high shear rate. On the grounds, we aimed to evaluate the association between WBV and CCC in patients with chronic total occlusion. Subjects and Methods: A total of 371 patients diagnosed as having at least one major, chronic total occluded coronary artery were included. 197 patients with good CCC (Rentrop 2 and 3) composed the patient group. The poor collateral group consisted of 174 patients (Rentrop grade 0 and 1). Results: Patients with poor CCC had higher WBV values for a low-shear rate (LSR) (69.5±8.7 vs. 60.1±9.8, p<0.001) and high-shear rate (HSR) (17.0±2.0 vs. 16.4±1.8, p<0.001) than the good collateral group. Correlation analysis demonstrated a significant negative correlation between the grade of CCC and WBV for LSR (β=0.597, p<0.001) and HSR (β=0.494, p<0.001). WBV for LSR (β=0.476, p<0.001) and HSR (β=0.407, p<0.001) had a significant correlation with the synergy between percutaneous coronary intervention with taxus and cardiac surgery (SYNTAX) score. A multivariate analysis showed that the WBV for both shear rates were independent risk factors of poor CCC (WBV at LSR, OR: 1.362 CI 95%: 1.095-1.741 p<0.001 and WBV at HSR, 1.251 CI 95%: 1.180-1.347 p<0.001). Conclusion: WBV has been demonstrated as the overlooked predictor of poor coronary collateralization. WBV seemed to be associated with microvascular perfusion and angiogenesis process impairing CCC development

Constituents of the Essential Oil of Sea Fennel (Crithmum maritimum L.) Growing Wild in Turkey

M. Musa Ozcan,Luis G. Pedro,A. Cristina Figueiredo,Jose G. Barroso 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.1

The essential oils isolated by hydrodistillation from freshly obtained aerial parts of sea fennel (Crithmum mar-itimumL.) were found to be a yellow liquid, obtained in a yield of 0.2% (vol/wt) based on wet weight. The essential oils ob-tained from young leaves and branches of C. maritimum, collected at two different locations in Turkey (Sipahili and Yesilo-vacik), were analyzed by gas chromatography-mass spectrometry. Twenty-three components were identified accounting formore than 99% of the total oils. The oils contained mainly monoterpenes, amounting to 89.0% and 99.6%, respectively, ofthe oils, with .-terpinene (36% and 32%), .-phellandrene (21% and 22%), and sabinene (13% and 9%) as the main compo-nents.

Composition of the Essential Oil of Helichrysum chasmolycicum Growing Wild in Turkey

M.M. Ozcan,J.C. Chalchat 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2

The chemical compositions of the essential oil obtained from the aerial parts of Helichrysum chasmolycicumwere analyzed by gas chromatography and gas chromatography-mass spectrometry. From the 57 identified constituents, rep-resenting 66.55% of the oil, the main constituents of the oil were .-caryophyllene (27.6%), .-selinene (8.9%), .-selinene(8.4%), caryophyllene oxide (7.3%), and carvacrol (2.4%). The essential oil was almost totally characterized by sesquiterpenehydrocarbons such as .-caryophyllene and .- and .-selinene.

Isomer-specific chromatographic profiling yields highly sensitive and specific potential N-glycan biomarkers for epithelial ovarian cancer

Hua, S.,Williams, C.C.,Dimapasoc, L.M.,Ro, G.S.,Ozcan, S.,Miyamoto, S.,Lebrilla, C.B.,An, H.J.,Leiserowitz, G.S. Elsevier 2013 Journal of chromatography A Vol.1279 No.-

Aberrant glycosylation has been observed for decades in essentially all types of cancer, and is now well established as an indicator of carcinogenesis. Mining the glycome for biomarkers, however, requires analytical methods that can rapidly separate, identify, and quantify isomeric glycans. We have developed a rapid-throughput method for chromatographic glycan profiling using microfluidic chip-based nanoflow liquid chromatography (nano-LC)/mass spectrometry. To demonstrate the utility of this method, we analyzed and compared serum samples from epithelial ovarian cancer cases (n=46) and healthy control individuals (n=48). Over 250 N-linked glycan compound peaks with over 100 distinct N-linked glycan compositions were identified. Statistical testing identified 26 potential glycan biomarkers based on both compositional and structure-specific analyses. Using these results, an optimized model was created incorporating the combined abundances of seven potential glycan biomarkers. The receiver operating characteristic (ROC) curve of this optimized model had an area under the curve (AUC) of 0.96, indicating robust discrimination between cancer cases and healthy controls. Rapid-throughput chromatographic glycan profiling was found to be an effective platform for structure-specific biomarker discovery.

May Long Term Oxcarbazepine Treatment Be Lead to Secondary Hyperparathyroidism?

O. Babacan,A. Karaoglu,S. Vurucu,E. Yesilkaya,O. Yesilyurt,T. Cayci,M. Gulgun,B. Unay,R. Akın,O. Ozcan 대한신경과학회 2012 Journal of Clinical Neurology Vol.8 No.1

Background and Purpose The adverse effects of newer antiepileptic drugs are not well-known. This study assessed the impact of oxcarbazepine (OXC) treatment on bone turnover. Methods Forty-four children with idiopathic focal (and/or secondarily generalized) epilepsy who had been treated with OXC for more than 1 year were compared with 33 healthy, age- and sexmatched children. Serum calcium, phosphorus, alkaline phosphatase, parathyroid hormone, osteocalcin, calcitonin, and 25-hydroxyvitamin D, and bone mineral density were measured to evaluate and compare bone mineralization between the two groups. Results The serum levels of calcium, osteocalcin, 25-hydroxyvitamin D, and bone mineral density did not differ significantly between the study and control groups. However, serum levels of parathyroid hormone, alkaline phosphatase, phosphorus, and calcitonin differed significantly between the two groups. Conclusions These findings suggest that OXC treatment leads to secondary hyperparathyroidism with high-turnover bone disease and/or impaired intestinal calcium absorption.

The Effect of Agoraphobia on Oxidative Stress in Panic Disorder

Isil Gogcegoz Gul,Rifat Karlidag,Birgul Elbozan Cumurcu,Yusuf Turkoz,Sukru Kartalci,A. Cemal Ozcan,M. Erman Erdemli 대한신경정신의학회 2013 PSYCHIATRY INVESTIGATION Vol.10 No.4

We aimed to investigate whether agoraphobia (A) in panic disorder (PD) has any effects on oxidative and anti-oxidative parameters. We measured total antioxidant capacity (TAC), paraoxonase (PON), arylesterase (ARE) antioxidant and malondialdehyde (MDA) oxidant levels using blood samples from a total of 31 PD patients with A, 22 PD patients without A and 53 control group subjects. There was a significant difference between the TAC, PON, ARE and MDA levels of the three groups consisting of PD with A, PD without A and the control group. The two-way comparison to clarify the group creating the difference showed that the TAC, PON, and ARE antioxidants were significantly lower in the PD with A group compared to the control group while the MDA oxidant was significantly higher. There was no significant difference between the PD without A and control groups for TAC, PON, ARE and MDA levels. We clearly demonstrated that the oxidative stress and damage to the anti-oxidative mechanism are significantly higher in the PD group with A. These findings suggest that oxidative/anti-oxidative mechanisms may play a more important role on the pathogenesis of PB with A.

Towards better insect management strategy: restriction of insecticidal gene expression to biting sites in transgenic cotton

Emine Anayol,Allah Bakhsh,Omer Cem Karakoc,Selma Onarıcı,Deniz Kom,Muhammad Aasim,Sancar Fatih Ozcan,Surendra Barpete,Saber D. Khabbazi,Burak Onol,Cengiz Sancak,Khalid M. Khawar,Levent Unlu,Sebahattin 한국식물생명공학회 2016 Plant biotechnology reports Vol.10 No.2

Most of the commercialized Bt crops express cry genes under 35S promoter that induces strong gene expression in all plant parts. However, targeted foreign gene expression in plants is esteemed more important as public may be likely to accept ‘less intrusive’ expression of transgene. We developed plant expression constructs harboring cry1Ac gene under control of wound-inducible promoter (AoPR1) to confine Bt gene expression in insect wounding parts of the plants in comparison with cry1Ac gene under the control of 35S promoter. The constructs were used to transform four Turkish cotton cultivars (GSN12, STN-468, Ozbek-100 and Ayhan-107) through Agrobacterium tumefaciens strains GV2260 containing binary vectors p35SAcBAR.101 and AoPR1AcBAR.101 harboring cry1Ac gene under control of 35S and AoPR1, respectively. Phosphinothricin (PPT) was used at concentration of 5 mg L-1 for selection of primary transformants. The primary transformants were analyzed for transgene presence and expression standard molecular techniques. The transformants exhibited appreciable mortality rates against larvae of Spodoptera exigua and S. littoralis. It was found that mechanical wounding of T1 transgenic plants was effective in inducing expression of cry1Ac protein as accumulated levels of cry1Ac protein increased during post-wounding period. We conclude that use of woundinducible promoter to drive insecticidal gene(s) can be regarded as a valuable insect-resistant management strategy since the promoter activity is limited to insect biting sites of plant. There is no Bt toxin accumulation in unwounded plant organs, seed and crop residues, cotton products and by-products, thus minimizing food and environmental concerns.