광주교육정보센터 - (주)류ㆍ한길 종합건축사사무소

류연창(Lyoo, Yun-chang) 현대건축사 2002 월간 CONCEPT Vol.- No.36

Genetic characterization of porcine circovirus-2 field isolates from PMWS pigs

Young S. Lyoo,YoungS.Lyoo 대한수의학회 2002 Journal of Veterinary Science Vol.3 No.1

PMWS is a new emerging disease in swine herdsworldwide. Field isolates of PCV-2, a putative majorcausative agent of PMWS, were isolated and geneticalycharacterized. Viral genome of two field isolates(PC201DJ and PC201SS) from pigs showing typicalPMWS was equenced. The nucleotide sequence homologywith other PCV-2 isolates was ranging from 95% to99% in complete viral genomic sequence. The highlywas identical to that of other PCV-2 isolates. Todetermine the genetic heterogeneity of PCV-2 isolates,t h e p h y l o g e n e t i c t r e e b a s e d o n t h e c o m p l e t e g e n o m eof PCV-2 isolates were constructed. Two PCV-2 fieldisolates were closely related to Canadian isolates ofPCV-2. PCV-2 isolated from field may have an originof North America nd is posibly originated fromimportation of breding stocks. The result indicatesthat although the genome of PCV-2 is relatively stablein general, minor genetic variations exist amongPCV-2 isolates from the diferent geographic locations.important implication for genetic characteristics ofPCV-2 infection. Thre major imunorelevant epitopesof capsid protein showed variations in amino acidsequences. Also, the variance of amino acid sequencein antigenic epitope xisted betwen two KoreanPCV-2 isolates.

Different cerebral cortical areas influence the effect of subthalamic nucleus stimulation on parkinsonian motor deficits and freezing of gait

Lyoo, Chul Hyoung,Aalto, Sargo,Rinne, Juha O,Lee, Ki Ook,Oh, Seung Hun,Chang, Jin Woo,Lee, Myung Sik Wiley Subscription Services, Inc., A Wiley Company 2007 Movement disorders Vol.22 No.15

<P>Inconsistent response in freezing of gait (FOG) with levodopa treatment or STN DBS makes the pathogenesis difficult to understand. We studied brain areas associated with the expression of STN DBS effect on parkinsonian motor deficits and FOG. Ten Parkinson's disease patients with typical FOG were included. One month before STN DBS, we performed [<SUP>18</SUP>F]-deoxyglucose PET scans and measured the UPDRS motor and modified FOG (mFOG) scores during levodopa off and on periods. At two months after STN DBS, same rating scores were measured. The percentage improvement of mFOG and UPDRS motor scores by STN DBS during levodopa off period was calculated. We searched for brain areas in which glucose metabolism correlated with the improvement of mFOG and UPDRS motor scores by DBS. During levodopa off period, STN DBS improved the UPDRS motor scores by 32.3% and the mFOG scores by 56.6%. There was no correlation between the improvements of both scores. The improvement of UPDRS motor score by DBS correlated with the metabolic activities of rostral supplementary motor area (Brodmann's area 8; BA8), anterior cingulate cortex (BA32), and prefrontal cortex (BA9). On the other hand, there was a positive correlation between the improvement of mFOG score by DBS and the metabolic activity of the parietal, occipital, and temporal sensory association cortices. In conclusion, dysfunction of different cerebral cortical areas limits the beneficial effects of DBS on parkinsonian motor deficits and FOG. © 2007 Movement Disorder Society</P>

Identification of porcine circoviruses with genetic variation from lymph nodes collected in pigs with PMWS

Lyoo, Young S.,Kim, Jin-hyun,Park, Choi-kyu The Korean Society of Veterinary Science 1999 大韓獸醫學會誌 Vol.39 No.2

New emerging post weaning multisystemic wasting syndrome (PMWS) threatening swine industry worldwide and investigation of the etiological agent is underway. The porcine circovirus (PCV) consistently identified from PMWS pigs and research result indicate that there is strong relationship between PCV and PMWS. Fauns with PMWS submitted pigs suffered from various PMWS typical signs and necropsy finding showed lymph node anomalies. The PCV DNA was amplified from inguinal lymph node collected from pigs with PMWS. PCV specific primers were successfully amplified PCV DNAs and were able to differentiate PCV type I and II. We have identified noble PCV virus with genetic variation. The virus showed insertion of the nucleic acid at the 5' of the genome but did not have PCR product with primer set corresponding to PCV type II virus.

Detection and genetic analysis of zoonotic hepatitis E virus, rotavirus, and sapovirus in pigs

Lyoo, Eu Lim,Park, Byung-Joo,Ahn, Hee-Seop,Han, Sang-Hoon,Go, Hyeon-Jeong,Kim, Dong-Hwi,Lee, Joong-Bok,Park, Seung-Yong,Song, Chang-Seon,Lee, Sang-Won,Choi, In-Soo The Korean Society of Veterinary Science 2020 大韓獸醫學會誌 Vol.60 No.2

The zoonotic transmission of viral diseases to humans is a serious public health concern. Pigs are frequently a major reservoir for several zoonotic viral diseases. Therefore, periodic surveillance is needed to determine the infection rates of zoonotic diseases in domestic pigs. Hepatitis E virus (HEV), rotavirus, sapovirus (SaV), and norovirus (NoV) are potential zoonotic viruses. In this study, 296 fecal samples were collected from weaned piglets and growing pigs in 13 swine farms, and the viral RNA was extracted. Partial viral genomes were amplified by reverse transcription-polymerase chain reaction (PCR) or nested-PCR using virus-specific primer sets under different PCR conditions. HEV-3, rotavirus A, and SaV genogoup 3 were detected from 11.5, 2.7, and 3.0% of the samples, respectively. On the other hand, NoV was not detected in any of the samples. Genetic analysis indicated that the nucleotide sequences of swine HEV-3 and rotavirus A detected in this study were closely related to those of human isolates. However, swine SaV was distant from the human strains. These results suggest that HEV-3 and rotavirus A can be transmitted from pigs to humans. Therefore, strict preventive measures should be implemented by workers in the swine industry to prevent infections with HEV-3 and rotavirus A excreted from pigs.

Virulence of a novel reassortant canine H3N2 influenza virus in ferret, dog and mouse models

Lyoo, K. S.,Na, W.,Yeom, M.,Jeong, D. G.,Kim, C. U.,Kim, J. K.,Song, D. Springer Science + Business Media 2016 Archives of virology Vol.161 No.7

<P>An outbreak of a canine influenza virus (CIV) H3N2 reassortant derived from pandemic (pdm) H1N1 and CIV H3N2 in companion animals has underscored the urgent need to monitor CIV infections for potential zoonotic transmission of influenza viruses to humans. In this study, we assessed the virulence of a novel CIV H3N2 reassortant, VC378, which was obtained from a dog that was coinfected with pdm H1N1 and CIV H3N2, in ferrets, dogs, and mice. Significantly enhanced virulence of VC378 was demonstrated in mice, although the transmissibility and pathogenicity of VC378 were similar to those of classical H3N2 in ferrets and dogs. This is notable because mice inoculated with an equivalent dose of classical CIV H3N2 showed no clinical signs and no lethality. We found that the PA and NS gene segments of VC378 were introduced from pdmH1N1, and these genes included the amino acid substitutions PA-P224S and NS-I123V, which were previously found to be associated with increased virulence in mice. Thus, we speculate that the natural reassortment between pdm H1N1 and CIV H3N2 can confer virulence and that continuous surveillance is needed to monitor the evolution of CIV in companion animals.</P>

Family Environment in Depressed Patients with Borderline Personality Disorder : Differences in Perception between patients and Parents

Lyoo,In Kyoon 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.5

연구목적 : 본 연구는 성인 境界線 人格障碍환자와, 환자와 心理的, 經濟的으로 밀접한 관계를 가지고 있는 부모와의 家族環境에 대한 認識의 차이를 알기 위함이다. 연구방법 : Structural Clinical Interview for DSM-Ⅲ-R과 Revised Diagnostic Interview for Personality Disorders를 사용하여 연구대상 집단으로 경계전 인격장애를 가진 주요 우울증 환자(N=24)와 이 집단의 정신과적 비교집단으로 경계선 인격장애를 가지지 않는 주요우울증 환자(N=21)를 선택하였다. 각 집단의 환자와 그 부모에게 Family Environment Scale을 시행한 후, 같은 집단 내에서 환자와 부모사이의 차이 그리고 부모간의 차이를 분석하였고, 두 집단의 구성원 사이의 가족환경에 대한 평가의 차이를 분석하였다. 연구결과 : 경계선 인격장애 환자집단은 결합력(Cohesion), 갈등(Conflict), 독립성(Independence)의 소척도에서 그들의 부모보다 부정적으로 가족환경을 인식했다. 환자의 父 와 母는 가족환경 인식에서 통계적으로 유의한 차이를 보이지 않았다. 경계선 인격장애를 가지지 않는 대조군에서는 환자와 그 부모사이 혹은 부모 서로 간에 Family Environment Scale의 어느 소척도에서도 통계적으로 유의한 차이가 없었다. 결 론 : 본 연구는 경계선 인격장애 환자와 그 부모사이의 가족환경에 대한 인식의 차이점을 구체적으로 파악하였다. 臨床的 측면에서는 이 차이점에 대한 치료적 접근의 필요성을 제기하였으며, 硏究적 측면에서는 지금까지 경계선 인격장애 환자의 시각과 회상에 주로 의존하여 환자의 가족환경, 가족관계 및 부모의 성격특성을 기술하고 정의하여 온 것에 대해서 재고가 필요함을 제시하였다.

Lithium-Induced Gray Matter Volume Increase As a Neural Correlate of Treatment Response in Bipolar Disorder: A Longitudinal Brain Imaging Study

Lyoo, In Kyoon,Dager, Stephen R,Kim, Jieun E,Yoon, Sujung J,Friedman, Seth D,Dunner, David L,Renshaw, Perry F American College of Neuropsychopharmacology 2010 Neuropsychopharmacology Vol.35 No.8

Preclinical studies suggest that lithium may exert neurotrophic effects that counteract pathological processes in the brain of patients with bipolar disorder (BD). To describe and compare the course and magnitude of gray matter volume changes in patients with BD who are treated with lithium or valproic acid (VPA) compared to healthy comparison subjects, and to assess clinical relationships to gray matter volume changes induced by lithium in patients with BD, we conducted longitudinal brain imaging and clinical evaluations of treatment response in 22 mood-stabilizing and antipsychotic medications-naive patients with BD who were randomly assigned to either lithium or VPA treatment after baseline assessment. Fourteen healthy comparison subjects did not take any psychotropic medications during follow-up. Longitudinal data analyses of 93 serial magnetic resonance images revealed lithium-induced increases in gray matter volume, which peaked at week 10–12 and were maintained through 16 weeks of treatment. This increase was associated with positive clinical response. In contrast, VPA-treated patients with BD or healthy comparison subjects did not show gray matter volume changes over time. Results suggest that lithium induces sustained increases in cerebral gray matter volume in patients with BD and that these changes are related to the therapeutic efficacy of lithium.

Personality Characteristics and Prevalence of Personality Disorders in Subjects with Bulimia Nervosa: Korean College Women in the United States of America

Lyoo, in Kyoon Seoul National University 1995 The seoul journal of medicine Vol.36 No.3

Preparation of High Molecular Weight Atactic Poly(vinyl alcohol) by Photo-induced Bulk Polymerization of Vinyl Acetate

Lyoo, Won-Seok,Ha, Wan-Shik The Korean Fiber Society 2001 Fibers and polymers Vol.2 No.2

Vinyl acetate was polymerized in ultraviolet-ray initiated bulk system at low temperatures using 2,2-azobis(2,4-dimethylvaleronitrile) (ADMVN) or 2,2-azobis(isobutyronitrile) (AIBN) as the photoinitiator, respectively. High molecular weight (HMW) poly(vinyl alcohol) (PVA) having number-average degree of polymerization ($P_n$) of 3,900-7,800 and syndiotactic diad (S-diad) content of 52.5-54.0% could be prepared by complete saponification of synthesized linear poly(vinyl acetate) (PVAc) having $P_n$ 5,900-9,400 obtained at conversion of below 30%. $P_n$ of PVA using ADMVN was larger than that of PVA using AIBN. On the other hand, conversion of the former was smaller than that of the latter, and it was found that the initiation rate of the ADMVN was lower than that of AIBN. This could be explained by a fact that the rate of photolysis of AIBN is faster than that of ADMVN due to the higher quantum yield or dissociation rate constant of AIBN than that of ADMVN. The $P_n$, syndiotacticity, and whiteness of PVA from PVAc polymerized at lower temperatures were superior to those of PVA from PVAc polymerized at higher temperatures.