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      • IL-4 increases type 2, but not type 1, cytokine production in CD8 <sup>+ </sup> T cells from mild atopic asthmatics

        Stanciu, Luminita A,Roberts, Kevan,Papadopoulos, Nikolaos G,Cho, Sang-Heon,Holgate, Stephen T,Coyle, Anthony J,Johnston, Sebastian L BioMed Central 2005 Respiratory research Vol.6 No.-

        <P><B>Background</B></P><P>Virus infections are the major cause of asthma exacerbations. CD8<SUP>+ </SUP>T cells have an important role in antiviral immune responses and animal studies suggest a role for CD8<SUP>+ </SUP>T cells in the pathogenesis of virus-induced asthma exacerbations. We have previously shown that the presence of IL-4 during stimulation increases the frequency of IL-5-positive cells and CD30 surface staining in CD8<SUP>+ </SUP>T cells from healthy, normal subjects. In this study, we investigated whether excess IL-4 during repeated TCR/CD3 stimulation of CD8<SUP>+ </SUP>T cells from atopic asthmatic subjects alters the balance of type 1/type 2 cytokine production in favour of the latter.</P><P><B>Methods</B></P><P>Peripheral blood CD8<SUP>+ </SUP>T cells from mild atopic asthmatic subjects were stimulated <I>in vitro </I>with anti-CD3 and IL-2 ± excess IL-4 and the expression of activation and adhesion molecules and type 1 and type 2 cytokine production were assessed.</P><P><B>Results</B></P><P>Surface expression of very late antigen-4 [VLA-4] and LFA-1 was decreased and the production of the type 2 cytokines IL-5 and IL-13 was augmented by the presence of IL-4 during stimulation of CD8<SUP>+ </SUP>T cells from mild atopic asthmatics.</P><P><B>Conclusion</B></P><P>These data suggest that during a respiratory virus infection activated CD8<SUP>+ </SUP>T cells from asthmatic subjects may produce excess type 2 cytokines and may contribute to asthma exacerbation by augmenting allergic inflammation.</P>

      • SCISCIESCOPUS

        Increased Interleukin-4, Interleukin-5, and Interferon-γ in Airway CD4<sup>+</sup>and CD8<sup>+</sup>T Cells in Atopic Asthma

        Cho, Sang-Heon,Stanciu, Luminita A.,Holgate, Stephen T.,Johnston, Sebastian L. American Thoracic Society 2005 American journal of respiratory and critical care Vol.171 No.3

        <P>Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4(+) T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8(+) T cell type 1 and type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-gamma, interleukin (IL)-4 and IL-5 producing CD4(+) and CD8(+) blood and sputum T cells from normal subjects and subjects with asthma and compared between cell subsets, subject groups, and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5, and IFN-gamma by unstimulated sputum CD4(+) and CD8(+) T cells was increased in subjects with asthma and related to disease severity, more for CD8(+) than for CD4(+) T cells. Frequencies of sputum CD8(+) T cells producing type 1 and type 2 cytokines were similar to those of CD4(+) T cells. In vitro stimulation polarized peripheral blood cytokine production toward IFN-gamma production, significantly more in subjects with asthma than in normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4(+) and CD8(+) T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.</P>

      • From Multimedia Data Mining to Multimedia Big Data Mining

        Constantin, Gradinaru Bogdanel,Mirela, Danubianu,Luminita, Barila Adina International Journal of Computer ScienceNetwork S 2022 International journal of computer science and netw Vol.22 No.11

        With the collection of huge volumes of text, image, audio, video or combinations of these, in a word multimedia data, the need to explore them in order to discover possible new, unexpected and possibly valuable information for decision making was born. Starting from the already existing data mining, but not as its extension, multimedia mining appeared as a distinct field with increased complexity and many characteristic aspects. Later, the concept of big data was extended to multimedia, resulting in multimedia big data, which in turn attracted the multimedia big data mining process. This paper aims to survey multimedia data mining, starting from the general concept and following the transition from multimedia data mining to multimedia big data mining, through an up-to-date synthesis of works in the field, which is a novelty, from our best of knowledge.

      • SCOPUSKCI등재

        Single Crystal Growth and Properties of 2D Antiferromagnet Ni<SUB>1-x</SUB>Zn<SUB>x</SUB>PS₃

        Nashra Pistawala,Ankit Kumar,Dibyata Rout,Luminita Harnagea,Surjeet Singh 한국물리학회 2023 새물리 Vol.73 No.12

        NiPS₃ is a rare Ni-based negative charge transfer insulator with an antiferromagnetically ordered ground state with Ni spins ordered in a zigzag configuration. Recent reports indicate the presence of strong spin-phonon and spin-charge coupling in NiPS₃. In this study, we report on the growth of high-quality single crystals of Ni<SUB>1-x</SUB>Zn<SUB>x</SUB>PS₃ (x = 0, 0.05, 0.08, 0.13, and 0.14) using the physical vapor transport method. The Raman spectra of all the compositions were measured at 300 K. We show that the phonon mode E<SUP>(2)</SUP><SUB>g</SUB> at 176 cm<SUP>-1</SUP> attributed to the vibration of Ni ions can only be well-fitted using the Breit-Wigner Fano line shape. The parameter |1/q|, which quantifies the strength of the coupling of the phonon E<SUP>(2)</SUP><SUB>g</SUB> to a broad continuum, is found to have a considerably high value of 0.13. The |1/q| parameter increases with increasing Zn doping. Our findings indicate the presence of charge-phonon coupling in NiPS3 at room temperature.

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