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Effect of Urolithin A on Bone Repair in Mice with Bone Defects
Liu Jianmin,Ma Longyang,Dong Wengang,Du Gongliang,Dang Xingbo 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.1
BACKGROUND: Bone defect difficult to manage clinically and it is a big challenge to repair it. Secondary metabolites source from herb has shown potential for the treatment of bone defect. METHODS: Mesenchymal stem cells (MSCs) were isolated from mice and incubated with urolithin A (UA) (10, 25, and 50 lg/mL). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was performed to estimate apoptosis and mineralisation was evaluated by alkaline phosphatase assay and alizarin red S staining. A middle femoral defect was induced in mice and bone tissue was prepared for endochondral ossification by treating with UA. The effect of UA was estimated by determining markers of osteoblast proliferation in serum and micro-computed tomography to analyse bone defects. RESULTS: UA enhanced mineralisation of MSCs and osteogenic gene markers in MSCs in vitro. Also, the bone defect score and bone mineral density were improved by UA. Moreover, UA ameliorated the altered Wnt3a protein and histopathological changes in bone defect mice. CONCLUSION: Presented report conclude that UA enhances osteoblast proliferation in bone-defect mice by activating the Wnt pathway.
Genetic dissection of leaf-related traits using 156 chromosomal segment substitution lines
Xi Liu,Linglong Liu,Yinhui Xiao,Shijia Liu,Yunlu Tian,Liangming Chen,Zhiquan Wang,Ling Jiang,Zhigang Zhao,Jianmin Wan 한국식물학회 2015 Journal of Plant Biology Vol.58 No.6
A two-line super-hybrid rice (Oryza sativa L.) variety [Liangyoupei9 (LYP9)] demonstrated superiority over its both parents, viz. elite inbred lines 93-11 and Pei-ai64S (PA64S), as well as other conventional hybrids, and had long been exploited in China. However, the genetic basis of its leaf-related traits, supposed to be an important component for yield potential, remains elusive. Here, initially a set of chromosome segment substitution lines (CSSLs) was constructed, in which the genome of Pei-ai64S has been introgressed into the background of 93-11. This set was developed by marker aided selection, based on 123 polymorphic SSR markers. The introgressed chromosomal segments presented in the 156 CSSLs covered 96.46% of Pei-ai64S genome. Afterwards, the CSSLs were deployed to assess the genetic basis of leaf size (length and width) and chlorophyll content of top three leaves across five different environments. The CSSLs showed transgressive segregation for all of the traits, and significant correlations were detected among most of the traits. A total of 27 quantitative trait loci (QTL) were identified on ten chromosomes, and three QTL cluster affecting related traits were found on chromosome 3, 6, and 8, respectively. Remarkably, two key QTLs, qALW3-1 and qALW3-2, both controlling the antepenultimate leaf width, were identified in all five environments, and their effect were further validated by CSSLs harboring the two QTL alleles. Our results indicate that developing CSSLs is a powerful tool for genetic dissection of quantitative traits. Meanwhile, the QTLs controlling leaf-related traits uncovered here provide useful information for marker-assisted selection in improving the performance of leaf morphology and photosynthetic ability.
Wuhua Long,Bangning Dong,Yihua Wang,Pengyi Pan,Yunlong Wang,Linglong Liu,Xiaoli Chen,Xi Liu,Shijia Liu,Yunlu Tian,Liangming Chen,Jianmin Wan 한국식물학회 2017 Journal of Plant Biology Vol.60 No.5
Cereal opaque-kernel mutants are ideal geneticmaterials for studying the mechanism of starch biosynthesisand amyloplast development. Here we isolated and identifiedtwo allelic floury endosperm 8 (flo8) mutants of rice, namedflo8-1 and flo8-2. In the flo8 mutant, the starch content wasdecreased and the normal physicochemical features ofstarch were altered. Map-based cloning and subsequentDNA sequencing analysis revealed a single nucleotidesubstitution and an 8-bp insertion occurred in UDP-glucosepyrophosphorylase 1 (Ugp1) gene in flo8-1 and flo8-2,respectively. Complementation of the flo8-1 mutant restorednormal seed appearance by expressing full length codingsequence of Ugp1. RT-qPCR analysis revealed that Ugp1was ubiquitously expressed. Mutation caused the decreasedUGPase activity and affected the expression of most of genesassociated with starch biosynthesis. Meanwhile, western blotand enzyme activity analyses showed the comparability ofprotein levels and enzyme activity of most tested starchbiosynthesis related genes. Our results demonstrate thatUgp1 plays an important role for starch biosynthesis in riceendosperm.
Jianmin Gu,Baipeng Yin,Shaoyan Fu,Cuihong Jin,Xin Liu,Zhenpan Bian,Jianjun Li,Lu Wang,Xiaoyu Li 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.2
Due to the intense influence of the shape and size of the photon building blocks on the limitation and guidance of opticalwaves, an important strategy is the fabrication of different structures. Herein, organic semiconductor tris-(8-hydroxyquinoline)aluminium (Alq3) nanostructures with controllable morphology, ranging from one-dimensional nanowires to twodimensionalplates, have been prepared through altering intermolecular interactions with employing the anti-solvent diffusioncooperate with solvent-volatilization induced self-assembly method. The morphologies of the formed nanostructures, whichare closely related to the stacking modes of the molecules, can be exactly controlled by altering the polarity of anti-solventsthat can influence various intermolecular interactions. The synthesis strategy reported here can potentially be extended toother functional organic nanomaterials.
Jianmin Wang,Fengqiu Liu,Sitian Qin 제어·로봇·시스템학회 2021 International Journal of Control, Automation, and Vol.19 No.6
A periodically intermittent control is considered for the memristor-based recurrent neural networks with disturbance and mixed time delays. The purpose of this study is to design the controller with less constraints, which is convenient for successful applications of memristor-based recurrent neural networks in complex environment. First, the estimate of the disturbance and mixed time delay are given by the assumptions. Then, by the method of Lyapunov-Krasovski functional, two new criteria ensuring globally exponential stabilization of the neural network are obtained under the controller, respectively. The proposed theoretical results indicate that the control width and control period are not constrained except that the control width has an upper bound. Furthermore, the design algorithm of controller is described. Finally, two examples are performed through four neural networks to illustrate the effectiveness of the proposed results.
Jinrong Liu,Fang Xie,Jianmin Wang,Weiwei Chen 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.7
We propose a new type of micro-channel turbulence promoter with micro-pores (MCTP-MPs) which is configured on the flat-sheet membrane surface to decrease membrane fouling and reduce energy consumption. The computational fluid dynamics (CFD) simulation and particle image velocimetry (PIV) experiment are employed to predict turbulent flow in a flat-sheet membrane channel equipped with MCTP-MPs. Velocity profiles and wall shear stresses on membrane surface obtained from PIV were compared with the simulated data to validate the reliability of the CFD simulation. The CFD simulation results and PIV experiment results showed that the corrugated MCTP of 300 μm micro-pores crosswise placed on the membrane surface could increase velocity and wall shear stress on the flat-sheet membrane surface, which improved the filtration flux, reduced concentration polarization and mitigated membrane fouling in the meantime.
Forced convection heat transfer of steam in a square ribbed channel
Jiazeng Liu,Jianmin Gao,Tieyu Gao 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.4
An experimental study of heat transfer characteristics of steam in a square channel (simulating a gas turbine blade cooling passage)with two opposite surfaces roughened by 60 deg parallel ribs was performed. The ranges of key governing parameters were: Reynolds numbers (Re) based on the channel hydraulic diameter (30000-140000), entry gauge pressure (0.2Mpa-0.5Mpa), heat flux of heat transfer surface area (5kWm-2-20kWm-2), and steam superheat (13℃-51℃). The test channel length was 1000mm, while the rib spacing (p/e)was 10, and the ratio of rib height (e) to hydraulic diameter (D) was 0.048. The test channel was heated by passing current through stainless steel walls instrumented with thermocouples. The local heat transfer coefficients on the ribbed wall from the channel entrance to the fully developed regions were measured. The semi-empirical correlation was fitted out by using the average Nusselt numbers in the fully developed region to cover the range of Reynolds number. The correlation can be used in the design of new generation of gas turbine blade cooled by steam.
Fengqiu Liu,Jianmin Wang,Hongxu Zhang,Pengfei Li 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.1
A new discrete-time neural network is proposed for solving convex quadratic programming problems with hybrid constraints. Based on the projection operator and convex optimization technologies, a single layer discrete-time neural network with monotonic descent dynamic step sizes is constructed. It is proved that the equilibrium points of the discrete-time neural network are globally exponentially convergent to the optimal solutions of the programming problem. Moreover, an algorithm is given based on the proposed neural network and the scheme of backtracking step-size adaptation. Finally, the proposed algorithm is applied to three types of quadratic programming problems and the gas oven identification via a support vector regression algorithm. The numerical experiments are performed to show the correctness and effectiveness of the results in this paper.