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Lisha K. P.,Debasis Sarkar 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.5
This study presents a detailed in silico analysisof bioethanol production from glucose/xylose mixtures ofvarious compositions by fed-batch co-culture and monoculturefermentation of specialized microbes. The monocultureconsists of recombinant Saccharomyces cerevisisethat can metabolize both hexose and pentose sugars whilethe co-culture system consists of substrate-selective microbes. Dynamic flux balance models based on available genomescalereconstructions of the microorganisms have beenused to analyze bioethanol production in fed-batch culturewith constant feed rates and the maximization of ethanolproductivity is addressed by computing optimal aerobicanaerobicswitching times. The simulation results clearlypoint to the superior performance of fed-batch fermentationof microbial co-culture against fed-batch fermentation ofmono-culture for bioethanol production from glucose/xylosemixtures. A set of potential genetic engineering strategiesfor enhancement of S. cerevisiae and Escherichia colistrains performance have been identified. Such in silicopredictions using genome-scale models provide valuableguidance for conducting in vivo metabolic engineeringexperiments.
Lisha P. Luke,M. B. Mohamed Sathik,Molly Thomas,Linu Kuruvilla,K.V. Sumesh 한국식물생명공학회 2017 JOURNAL OF PLANT BIOTECHNOLOGY Vol.44 No.2
Drought stress is one of the important factors that restrict the expansion of Hevea brasiliensis cultivation to nontraditional regions experiencing extreme weather conditions. Plants respond to drought stress by triggering expression of several drought responsive genes including transcription factors which in turn trigger expression of various downstream signalling pathways and adaptive networks. Expression of such drought responsive genes may revert back to their original level upon re-watering. However, no reports are available on such phenomenon in Hevea and hence, this study was initiated. For this purpose, NAC transcription factor (NAC tf) was chosen as candidate gene. Its expression levels were monitored under intermittent drought as well as irrigated conditions in two clones (RRII 105 and RRIM 600) of H. brasiliensis with contrasting tolerance level. Copy number of NAC tf was found similar in both the clones. Expression of NAC tf was found highly up-regulated in RRIM 600 (a relatively drought tolerant clone) than in RRII 105 (a relatively drought susceptible clone) throughout the drought incidences which upon re-watering, reached back to its original levels in both the clones. The study indicated the existence of an association between expression of NAC tf and drought tolerance trait exhibited by the tolerant clone RRIM 600. The study also proves the influence of drought and re-watering on the leaf photosynthesis and expression of NAC tf in H. brasiliensis.
Luke, Lisha P.,Sathik, M.B. Mohamed,Thomas, Molly,Kuruvilla, Linu,Sumesh, K.V. The Korean Society of Plant Biotechnology 2017 식물생명공학회지 Vol.26 No.4
Drought stress is one of the important factors that restrict the expansion of Hevea brasiliensis cultivation to non-traditional regions experiencing extreme weather conditions. Plants respond to drought stress by triggering expression of several drought responsive genes including transcription factors which in turn trigger expression of various downstream signalling pathways and adaptive networks. Expression of such drought responsive genes may revert back to their original level upon re-watering. However, no reports are available on such phenomenon in Hevea and hence, this study was initiated. For this purpose, NAC transcription factor (NAC tf) was chosen as candidate gene. Its expression levels were monitored under intermittent drought as well as irrigated conditions in two clones (RRII 105 and RRIM 600) of H. brasiliensis with contrasting tolerance level. Copy number of NAC tf was found similar in both the clones. Expression of NAC tf was found highly up-regulated in RRIM 600 (a relatively drought tolerant clone) than in RRII 105 (a relatively drought susceptible clone) throughout the drought incidences which upon re-watering, reached back to its original levels in both the clones. The study indicated the existence of an association between expression of NAC tf and drought tolerance trait exhibited by the tolerant clone RRIM 600. The study also proves the influence of drought and re-watering on the leaf photosynthesis and expression of NAC tf in H. brasiliensis.